Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2002-06-28

AUTHORS

Denise L. Doolan , J. David Haynes , J. Kathleen Moch

ABSTRACT

Continuously automated synchronization of Plasmodium falciparum malaria parasites can be achieved by culturing in a custom temperature-cycling incubator (TCI) whose process controller is programmed to periodically change between three different temperatures: 1. Shortly after invasion of merozoites into erythrocytes at about 37°C, the culture temperature is lowered to about 17°C, and the maturation of ring forms (Rgearly?Rglate) is suspended (and perhaps some remaining schizonts are killed). The time ramping down to and holding at 17°C, generally between 2.5 and 7.25 h, is determined by adding times for the next three steps below and subtracting from 48 hr. Thus, the total cycle length for any given parasite isolate is adjusted to 48 h, even though various free-cycle times at 37°C for different isolates range from about 38 to 45 h. 2. When the culture temperature is again raised to about 37°C, ring stages mature normally to trophozoites with pigment. Depending on the characteristics of the parasite isolate and its adaptation to culture, we allow between 12.75 and 18.5 h for this (including time for ramping to temperature). 3. When the culture temperature is raised to about 40°C, trophozoites (Tr) continue to mature up to a point (Trearly?Trlate), but then further maturation to schizonts (Trlate?Sz) is blocked, so that parasites at earlier stages catch up to those at later stages. We allow between 9.5 and 10.5 h for this. 4. When the culture temperature is lowered again to about 37°C, maturation to schizonts (Trlate?Sz) proceeds, and merozoites are released and invade erythrocytes. We allow between 18.5 and 22.5 h for this. More... »

PAGES

489-498

Book

TITLE

Malaria Methods and Protocols

ISBN

1-59259-271-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-271-6:489

DOI

http://dx.doi.org/10.1385/1-59259-271-6:489

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049729428


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "Doolan", 
        "givenName": "Denise L.", 
        "id": "sg:person.01114332302.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114332302.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Immunology, Walter Reed Army Institute of Research", 
            "Malaria Program, Naval Medical Research Center"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Haynes", 
        "givenName": "J. David", 
        "id": "sg:person.014510037362.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014510037362.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Malaria Program, Naval Medical Research Center"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moch", 
        "givenName": "J. Kathleen", 
        "id": "sg:person.0723471026.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723471026.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1084/jem.169.1.357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012111816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(89)90550-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032047125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(89)90550-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032047125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0031182098002893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043544767"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0031182098002893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043544767"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-06-28", 
    "datePublishedReg": "2002-06-28", 
    "description": "Continuously automated synchronization of Plasmodium falciparum malaria parasites can be achieved by culturing in a custom temperature-cycling incubator (TCI) whose process controller is programmed to periodically change between three different temperatures: 1. Shortly after invasion of merozoites into erythrocytes at about 37\u00b0C, the culture temperature is lowered to about 17\u00b0C, and the maturation of ring forms (Rgearly?Rglate) is suspended (and perhaps some remaining schizonts are killed). The time ramping down to and holding at 17\u00b0C, generally between 2.5 and 7.25 h, is determined by adding times for the next three steps below and subtracting from 48 hr. Thus, the total cycle length for any given parasite isolate is adjusted to 48 h, even though various free-cycle times at 37\u00b0C for different isolates range from about 38 to 45 h. \u00a02. When the culture temperature is again raised to about 37\u00b0C, ring stages mature normally to trophozoites with pigment. Depending on the characteristics of the parasite isolate and its adaptation to culture, we allow between 12.75 and 18.5 h for this (including time for ramping to temperature). \u00a03. When the culture temperature is raised to about 40\u00b0C, trophozoites (Tr) continue to mature up to a point (Trearly?Trlate), but then further maturation to schizonts (Trlate?Sz) is blocked, so that parasites at earlier stages catch up to those at later stages. We allow between 9.5 and 10.5 h for this. \u00a04. When the culture temperature is lowered again to about 37\u00b0C, maturation to schizonts (Trlate?Sz) proceeds, and merozoites are released and invade erythrocytes. We allow between 18.5 and 22.5 h for this. \u00a0", 
    "genre": "chapter", 
    "id": "sg:pub.10.1385/1-59259-271-6:489", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "1-59259-271-6"
      ], 
      "name": "Malaria Methods and Protocols", 
      "type": "Book"
    }, 
    "name": "Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator", 
    "pagination": "489-498", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1385/1-59259-271-6:489"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ece4e859288ea3ccd1acf708401ce750dab13ddd7b9da858f8541d0a940d6184"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049729428"
        ]
      }
    ], 
    "publisher": {
      "location": "New Jersey", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1385/1-59259-271-6:489", 
      "https://app.dimensions.ai/details/publication/pub.1049729428"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T15:24", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000274.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1385/1-59259-271-6:489"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1385/1-59259-271-6:489'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1385/1-59259-271-6:489'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1385/1-59259-271-6:489'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1385/1-59259-271-6:489'


 

This table displays all metadata directly associated to this object as RDF triples.

82 TRIPLES      22 PREDICATES      28 URIs      18 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1385/1-59259-271-6:489 schema:about anzsrc-for:11
2 anzsrc-for:1108
3 schema:author Ne0f05535cbfe4e0b8a33237aebf34d61
4 schema:citation https://doi.org/10.1016/0006-2952(89)90550-9
5 https://doi.org/10.1017/s0031182098002893
6 https://doi.org/10.1084/jem.169.1.357
7 schema:datePublished 2002-06-28
8 schema:datePublishedReg 2002-06-28
9 schema:description Continuously automated synchronization of Plasmodium falciparum malaria parasites can be achieved by culturing in a custom temperature-cycling incubator (TCI) whose process controller is programmed to periodically change between three different temperatures: 1. Shortly after invasion of merozoites into erythrocytes at about 37°C, the culture temperature is lowered to about 17°C, and the maturation of ring forms (Rgearly?Rglate) is suspended (and perhaps some remaining schizonts are killed). The time ramping down to and holding at 17°C, generally between 2.5 and 7.25 h, is determined by adding times for the next three steps below and subtracting from 48 hr. Thus, the total cycle length for any given parasite isolate is adjusted to 48 h, even though various free-cycle times at 37°C for different isolates range from about 38 to 45 h.  2. When the culture temperature is again raised to about 37°C, ring stages mature normally to trophozoites with pigment. Depending on the characteristics of the parasite isolate and its adaptation to culture, we allow between 12.75 and 18.5 h for this (including time for ramping to temperature).  3. When the culture temperature is raised to about 40°C, trophozoites (Tr) continue to mature up to a point (Trearly?Trlate), but then further maturation to schizonts (Trlate?Sz) is blocked, so that parasites at earlier stages catch up to those at later stages. We allow between 9.5 and 10.5 h for this.  4. When the culture temperature is lowered again to about 37°C, maturation to schizonts (Trlate?Sz) proceeds, and merozoites are released and invade erythrocytes. We allow between 18.5 and 22.5 h for this.  
10 schema:genre chapter
11 schema:inLanguage en
12 schema:isAccessibleForFree false
13 schema:isPartOf N60ffa3b21dec48a088200537da3c2f5c
14 schema:name Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator
15 schema:pagination 489-498
16 schema:productId N6d9929b1dd55455eba6da39efb196c6b
17 Nedc26c719d53429086b9b62b301fca8f
18 Nf2f1d303d49d4ae5a82342ddcdce88c1
19 schema:publisher N550c0a07a0304b0f893aa04e64ad6732
20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049729428
21 https://doi.org/10.1385/1-59259-271-6:489
22 schema:sdDatePublished 2019-04-15T15:24
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher N0f82158d29cf4e8cbd40f5e04a94580d
25 schema:url http://link.springer.com/10.1385/1-59259-271-6:489
26 sgo:license sg:explorer/license/
27 sgo:sdDataset chapters
28 rdf:type schema:Chapter
29 N0987d4e8c459482fa5079e7abb114221 schema:name Malaria Program, Naval Medical Research Center
30 rdf:type schema:Organization
31 N0f82158d29cf4e8cbd40f5e04a94580d schema:name Springer Nature - SN SciGraph project
32 rdf:type schema:Organization
33 N2ab4eb01f9ce42a5a08da04bf4275676 schema:name Department of Immunology, Walter Reed Army Institute of Research
34 Malaria Program, Naval Medical Research Center
35 rdf:type schema:Organization
36 N2ac731be3a7c4bb4a7ea173f1e8011a7 rdf:first sg:person.0723471026.80
37 rdf:rest rdf:nil
38 N550c0a07a0304b0f893aa04e64ad6732 schema:location New Jersey
39 schema:name Humana Press
40 rdf:type schema:Organisation
41 N60ffa3b21dec48a088200537da3c2f5c schema:isbn 1-59259-271-6
42 schema:name Malaria Methods and Protocols
43 rdf:type schema:Book
44 N6d9929b1dd55455eba6da39efb196c6b schema:name dimensions_id
45 schema:value pub.1049729428
46 rdf:type schema:PropertyValue
47 Ne0f05535cbfe4e0b8a33237aebf34d61 rdf:first sg:person.01114332302.48
48 rdf:rest Neee6142986fa42ab807c08b7a9c92115
49 Nedc26c719d53429086b9b62b301fca8f schema:name readcube_id
50 schema:value ece4e859288ea3ccd1acf708401ce750dab13ddd7b9da858f8541d0a940d6184
51 rdf:type schema:PropertyValue
52 Neee6142986fa42ab807c08b7a9c92115 rdf:first sg:person.014510037362.91
53 rdf:rest N2ac731be3a7c4bb4a7ea173f1e8011a7
54 Nf2f1d303d49d4ae5a82342ddcdce88c1 schema:name doi
55 schema:value 10.1385/1-59259-271-6:489
56 rdf:type schema:PropertyValue
57 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
58 schema:name Medical and Health Sciences
59 rdf:type schema:DefinedTerm
60 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
61 schema:name Medical Microbiology
62 rdf:type schema:DefinedTerm
63 sg:person.01114332302.48 schema:familyName Doolan
64 schema:givenName Denise L.
65 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114332302.48
66 rdf:type schema:Person
67 sg:person.014510037362.91 schema:affiliation N2ab4eb01f9ce42a5a08da04bf4275676
68 schema:familyName Haynes
69 schema:givenName J. David
70 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014510037362.91
71 rdf:type schema:Person
72 sg:person.0723471026.80 schema:affiliation N0987d4e8c459482fa5079e7abb114221
73 schema:familyName Moch
74 schema:givenName J. Kathleen
75 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723471026.80
76 rdf:type schema:Person
77 https://doi.org/10.1016/0006-2952(89)90550-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032047125
78 rdf:type schema:CreativeWork
79 https://doi.org/10.1017/s0031182098002893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043544767
80 rdf:type schema:CreativeWork
81 https://doi.org/10.1084/jem.169.1.357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012111816
82 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...