Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopy View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2001-03-27

AUTHORS

Evgeny N. Vulfson , Peter J. Halling , Herbert L. Holland , Hideo Kise

ABSTRACT

The ability of enzymes to catalyze synthetic reactions in mainly organic solvents has been well documented. In general, enzymes are insoluble in organic solvents. Therefore, dispersions of hydrated enzymes or immobilized enzymes have been most extensively used for synthetic reactions in organic solvents. In these reaction systems, the nature of organic solvent and water-organic ratio strongly influence the activity and specificity of enzymes. It is reasonably assumed that changes in activity and specificity of enzymes are mainly ascribed to changes in the higher structures of enzymes. Therefore, analysis of enzyme structure is important for the selection of the solvent, but methods to analyze dispersed or immobilized enzymes in organic solvents are rather limited. More... »

PAGES

203-211

References to SciGraph publications

Book

TITLE

Enzymes in Nonaqueous Solvents

ISBN

1-59259-112-4

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-112-4:203

DOI

http://dx.doi.org/10.1385/1-59259-112-4:203

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014439072


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