In Vivo Gene Electroporation in the Mouse Testis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2000-02-18

AUTHORS

Mark J. Jaroszeski , Richard Heller , Richard Gilbert , Tatsuo Muramatsu

ABSTRACT

Until today, gene transfer to germ cells has been attempted by a variety of methods including microinjection, embryonic stem cell-mediated transfection, virus mediated transfection, lipofection (1), microparticle bombardment (2), and sperm mediated transformation (3). In vivo gene electroporation (EP) now is a viable option for gene transfer purposes as demonstrated by strong shortterm gene expression and long-term expression after gene transfer (4,5 and unpublished). In vivo gene EP is simple and convenient. Adequate development and differentiation of transfected cells can be maintained in the in vivo environment. Furthermore, it can be applied, in principle, to any types of cells and tissues so long as the target is accessible. The advantages of in vivo EP over other nonviral methods such as lipofection and microparticle bombardment are: possible tissue damage is less; there is no limitation of DNA to be transfected at a time; and DNA can be transferable to cells deep inside the target tissue. More... »

PAGES

349-57

References to SciGraph publications

  • 1995-06-20. Instrumentation in ELECTROPORATION PROTOCOLS FOR MICROORGANISMS
  • Book

    TITLE

    Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery

    ISBN

    1-59259-080-2

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1385/1-59259-080-2:349

    DOI

    http://dx.doi.org/10.1385/1-59259-080-2:349

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024599800

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21445753


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