In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2000-02-18

AUTHORS

Mark J. Jaroszeski , Richard Heller , Richard Gilbert , Sek Wen Hui , Lin Hong Li

ABSTRACT

Electroporation generally refers to the technique of permeabilizing cell membranes by applying a short and intense electric pulse across a cell, such that the barrier function of the membrane is instantaneously compromised. During such time, genetic materials may travel across the membrane. For a successful gene transfer process, the barrier function of the cell membrane is rapidly restored, and the cell survives. The electrotransfection process thus comprises two steps. The first step is electroporation, which is governed by the electrical properties of the cell and the suspension medium. The controlling parameters are mainly electrical. The second step is recovery, which must take into account the biological characteristics of the cells. We consider these two steps in this chapter. More... »

PAGES

157-71

Book

TITLE

Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery

ISBN

1-59259-080-2

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-080-2:157

DOI

http://dx.doi.org/10.1385/1-59259-080-2:157

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031616543

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21445734


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