Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2000-11-07

AUTHORS

Carrie G. Causing , Freda D. Miller

ABSTRACT

The development and analysis of transgenic mice that overexpress or misexpress neurotrophins or their receptors has led to a number of fundamental insights into the mechanisms whereby this family of growth factors exerts its biological effects in vivo (1–5). These transgenic mice were generated when an appropriate foreign DNA construct, or transgene, expressing a neurotrophin or neurotrophin receptor was stably incorporated in the genome of every cell; the transgene, which was incorporated at the same chromosomal location(s) in germ-line and somatic cells, was transmitted to offspring in a Mendelian manner, allowing for a detailed analysis of the physiological consequences of perturbations in neurotrophin biology. Although conceptually simple, the generation and analysis of transgenic mice is a time-consuming and expensive process that is fraught with potential conceptual and technical problems. In this chapter, we provide (1) an overview of the steps involved in the production and analysis of transgenic mice that overexpress neurotrophins or their receptors, with an emphasis on targetting to neurons, and (2) detailed protocols for the characterization of transgenic mice at the level of DNA, RNA, and protein expression. More... »

PAGES

167-191

Book

TITLE

Neurotrophin Protocols

ISBN

978-0-89603-699-4
978-1-59259-060-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-060-8:167

DOI

http://dx.doi.org/10.1385/1-59259-060-8:167

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047273710


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Canada", 
          "id": "http://www.grid.ac/institutes/grid.14709.3b", 
          "name": [
            "Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Causing", 
        "givenName": "Carrie G.", 
        "id": "sg:person.01160366165.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160366165.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Canada", 
          "id": "http://www.grid.ac/institutes/grid.14709.3b", 
          "name": [
            "Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "Freda D.", 
        "id": "sg:person.011535750057.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011535750057.00"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2000-11-07", 
    "datePublishedReg": "2000-11-07", 
    "description": "The development and analysis of transgenic mice that overexpress or misexpress neurotrophins or their receptors has led to a number of fundamental insights into the mechanisms whereby this family of growth factors exerts its biological effects in vivo (1\u20135). These transgenic mice were generated when an appropriate foreign DNA construct, or transgene, expressing a neurotrophin or neurotrophin receptor was stably incorporated in the genome of every cell; the transgene, which was incorporated at the same chromosomal location(s) in germ-line and somatic cells, was transmitted to offspring in a Mendelian manner, allowing for a detailed analysis of the physiological consequences of perturbations in neurotrophin biology. Although conceptually simple, the generation and analysis of transgenic mice is a time-consuming and expensive process that is fraught with potential conceptual and technical problems. In this chapter, we provide (1) an overview of the steps involved in the production and analysis of transgenic mice that overexpress neurotrophins or their receptors, with an emphasis on targetting to neurons, and (2) detailed protocols for the characterization of transgenic mice at the level of DNA, RNA, and protein expression.", 
    "genre": "chapter", 
    "id": "sg:pub.10.1385/1-59259-060-8:167", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-0-89603-699-4", 
        "978-1-59259-060-5"
      ], 
      "name": "Neurotrophin Protocols", 
      "type": "Book"
    }, 
    "keywords": [
      "transgenic mice", 
      "level of DNA", 
      "somatic cells", 
      "neurotrophin biology", 
      "Mendelian manner", 
      "detailed protocol", 
      "DNA constructs", 
      "physiological consequences", 
      "protein expression", 
      "growth factor", 
      "biological effects", 
      "receptors", 
      "neurotrophin receptor", 
      "genome", 
      "neurotrophins", 
      "cells", 
      "fundamental insights", 
      "mice", 
      "chromosomal", 
      "RNA", 
      "biology", 
      "transgene", 
      "DNA", 
      "expression", 
      "offspring", 
      "vivo", 
      "family", 
      "detailed analysis", 
      "insights", 
      "neurons", 
      "mechanism", 
      "analysis", 
      "expensive process", 
      "production", 
      "characterization", 
      "manner", 
      "constructs", 
      "perturbations", 
      "consequences", 
      "development", 
      "levels", 
      "technical problems", 
      "factors", 
      "step", 
      "protocol", 
      "generation", 
      "number", 
      "effect", 
      "process", 
      "chapter", 
      "overview", 
      "emphasis", 
      "construction", 
      "problem"
    ], 
    "name": "Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors", 
    "pagination": "167-191", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1047273710"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1385/1-59259-060-8:167"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1385/1-59259-060-8:167", 
      "https://app.dimensions.ai/details/publication/pub.1047273710"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-12-01T06:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/chapter/chapter_249.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1385/1-59259-060-8:167"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1385/1-59259-060-8:167'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1385/1-59259-060-8:167'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1385/1-59259-060-8:167'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1385/1-59259-060-8:167'


 

This table displays all metadata directly associated to this object as RDF triples.

114 TRIPLES      21 PREDICATES      76 URIs      69 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1385/1-59259-060-8:167 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N63d0c8f6d69f4136bf617a8257678f7a
4 schema:datePublished 2000-11-07
5 schema:datePublishedReg 2000-11-07
6 schema:description The development and analysis of transgenic mice that overexpress or misexpress neurotrophins or their receptors has led to a number of fundamental insights into the mechanisms whereby this family of growth factors exerts its biological effects in vivo (1–5). These transgenic mice were generated when an appropriate foreign DNA construct, or transgene, expressing a neurotrophin or neurotrophin receptor was stably incorporated in the genome of every cell; the transgene, which was incorporated at the same chromosomal location(s) in germ-line and somatic cells, was transmitted to offspring in a Mendelian manner, allowing for a detailed analysis of the physiological consequences of perturbations in neurotrophin biology. Although conceptually simple, the generation and analysis of transgenic mice is a time-consuming and expensive process that is fraught with potential conceptual and technical problems. In this chapter, we provide (1) an overview of the steps involved in the production and analysis of transgenic mice that overexpress neurotrophins or their receptors, with an emphasis on targetting to neurons, and (2) detailed protocols for the characterization of transgenic mice at the level of DNA, RNA, and protein expression.
7 schema:genre chapter
8 schema:isAccessibleForFree false
9 schema:isPartOf Na9604dc027214377987a0f625e86284f
10 schema:keywords DNA
11 DNA constructs
12 Mendelian manner
13 RNA
14 analysis
15 biological effects
16 biology
17 cells
18 chapter
19 characterization
20 chromosomal
21 consequences
22 construction
23 constructs
24 detailed analysis
25 detailed protocol
26 development
27 effect
28 emphasis
29 expensive process
30 expression
31 factors
32 family
33 fundamental insights
34 generation
35 genome
36 growth factor
37 insights
38 level of DNA
39 levels
40 manner
41 mechanism
42 mice
43 neurons
44 neurotrophin biology
45 neurotrophin receptor
46 neurotrophins
47 number
48 offspring
49 overview
50 perturbations
51 physiological consequences
52 problem
53 process
54 production
55 protein expression
56 protocol
57 receptors
58 somatic cells
59 step
60 technical problems
61 transgene
62 transgenic mice
63 vivo
64 schema:name Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors
65 schema:pagination 167-191
66 schema:productId N7b10de3d4fa04b2dac35918939c13543
67 Nc4c18395c53e4b139a20dfc25658a4f8
68 schema:publisher N1253808db6004d9db90b0a9fc156ade9
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047273710
70 https://doi.org/10.1385/1-59259-060-8:167
71 schema:sdDatePublished 2022-12-01T06:49
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N4ea5df5651124f6896ebcfd4589cc393
74 schema:url https://doi.org/10.1385/1-59259-060-8:167
75 sgo:license sg:explorer/license/
76 sgo:sdDataset chapters
77 rdf:type schema:Chapter
78 N1253808db6004d9db90b0a9fc156ade9 schema:name Springer Nature
79 rdf:type schema:Organisation
80 N4ea5df5651124f6896ebcfd4589cc393 schema:name Springer Nature - SN SciGraph project
81 rdf:type schema:Organization
82 N6281437a64fb45d78e3206880add652d rdf:first sg:person.011535750057.00
83 rdf:rest rdf:nil
84 N63d0c8f6d69f4136bf617a8257678f7a rdf:first sg:person.01160366165.47
85 rdf:rest N6281437a64fb45d78e3206880add652d
86 N7b10de3d4fa04b2dac35918939c13543 schema:name dimensions_id
87 schema:value pub.1047273710
88 rdf:type schema:PropertyValue
89 Na9604dc027214377987a0f625e86284f schema:isbn 978-0-89603-699-4
90 978-1-59259-060-5
91 schema:name Neurotrophin Protocols
92 rdf:type schema:Book
93 Nc4c18395c53e4b139a20dfc25658a4f8 schema:name doi
94 schema:value 10.1385/1-59259-060-8:167
95 rdf:type schema:PropertyValue
96 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
97 schema:name Biological Sciences
98 rdf:type schema:DefinedTerm
99 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
100 schema:name Biochemistry and Cell Biology
101 rdf:type schema:DefinedTerm
102 sg:person.011535750057.00 schema:affiliation grid-institutes:grid.14709.3b
103 schema:familyName Miller
104 schema:givenName Freda D.
105 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011535750057.00
106 rdf:type schema:Person
107 sg:person.01160366165.47 schema:affiliation grid-institutes:grid.14709.3b
108 schema:familyName Causing
109 schema:givenName Carrie G.
110 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160366165.47
111 rdf:type schema:Person
112 grid-institutes:grid.14709.3b schema:alternateName Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Canada
113 schema:name Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Canada
114 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...