Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared ... View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2000-09-05

AUTHORS

Kang-Lyung Woo

ABSTRACT

Amino acid analysis with reverse-phase high-performance liquid chromatography (RP-HPLC) and ultraviolet (UV) detection following precolumn derivatization is popular owing to the greater versatility at the instrument, sensitivity and speed of analysis compared to specialized ion-exchange amino acid analyzers. Phenylthiocarbamyl (PTC) amino acid derivative is a precolumn derivatization method that has been widely used for analysis of amino acid by RP-HPLC (1–8). This method is an excellent method for the derivatization of secondary amino acids, proline, and hydroxyproline (2–4,7). However, its disadvantages are that it requires a high-vacuum system and it takes a long time to remove the byproducts produced in the process of derivatization and the excess reagent in order to avoid interfering peaks. More... »

PAGES

141-167

Book

TITLE

Amino Acid Analysis Protocols

ISBN

978-0-89603-656-7
978-1-59259-047-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-047-0:141

DOI

http://dx.doi.org/10.1385/1-59259-047-0:141

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017690034


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