Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent) View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2000-09-05

AUTHORS

Sunil Kochhar , Barbara Mouratou , Philipp Christen

ABSTRACT

Precolumn derivatization of amino acids followed by their resolution by reverse-phase high-performance liquid chromatography (RP-HPLC) is now the preferred method for quantitative amino acid analysis. The derivatization step introduces covalently bound chromophores necessary not only for interactions with the apolar stationary phase for high resolution, but also for photometric or fluorometric detection. More... »

PAGES

49-54

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-047-0:049

DOI

http://dx.doi.org/10.1385/1-59259-047-0:049

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003425385


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