Transposon Tagging with AC/Ds in Arabidopsis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1998

AUTHORS

Deborah Long , George Coupland

ABSTRACT

Transposon tagging provides an effective method of isolating plant genes by identifying a mutation tagged by a DNA insertion, and then using the DNA tag to isolate the mutant gene (reviewed m ref. I). The first examples of this approach in plants involved transposon tagging in maize and Antirrhinum using endogenous transposons. More recently, maize transposons were shown to be active in a wide range of plant species, and in some of these have facilitated the use of transposon tagging (2‐5). In Arabidopsis, Actzvator/Dissoclation (AC/ Ds) have been used to isolate recessive loss-of-function mutations (6—8), dommant gain of function mutations (9) and to isolate genes based on then patterns of expression (10,11). More... »

PAGES

315-328

Book

TITLE

Arabidopsis Protocols

ISBN

978-0-89603-391-7
978-1-59259-268-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/0-89603-391-0:315

DOI

http://dx.doi.org/10.1385/0-89603-391-0:315

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013885025


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