Flow Cytometric Detection and Quantitation of Cell–Cell Electrofusion Products View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1997-11-26

AUTHORS

Mark J. Jaroszeski , Richard Heller , Richard Gilbert

ABSTRACT

Cell-cell electrofusion (CCE) is a process that involves forcing cells into close juxtaposition and then inducing fusion by delivering electric pulses to the cells. CCE has proven to have many practical applications. It has been used for monoclonal antibody (Mab) production (4,5), hybridoma production (1–3), and membrane surface marker transfer (6). Many other applications are described in this volume. In addition, the study of membrane fusion mechanisms has been the focus of some researchers (7–12). Electrofusion techniques seldom result in 100% yields between fusion partners. Therefore, a major aspect of all CCE applications is the ability to detect and quantitate fusion products. More... »

PAGES

149-156

References to SciGraph publications

Book

TITLE

Flow Cytometry Protocols

ISBN

0-89603-354-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/0-89603-354-6:149

DOI

http://dx.doi.org/10.1385/0-89603-354-6:149

DIMENSIONS

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