PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996-01-22

AUTHORS

Daniel C. Tessier , David Y. Thomas

ABSTRACT

We present in this chapter a polymerase chain reaction (PCR)-based method to simultaneously introduce and remove large fragments of DNA in a single mutagenesis reaction without the need for restriction sites. We have favored the use of long single-stranded DNA primers synthesized by asymmetric PCR (1,2), whereas others have used double-stranded PCR-amplified fragment directly (3–5) as primers in in vitro mutagenesis reactions. More... »

PAGES

229-237

Book

TITLE

In Vitro Mutagenesis Protocols

ISBN

978-0-89603-332-0
978-1-59259-544-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/0-89603-332-5:229

DOI

http://dx.doi.org/10.1385/0-89603-332-5:229

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009409637


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada", 
          "id": "http://www.grid.ac/institutes/grid.443931.8", 
          "name": [
            "National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tessier", 
        "givenName": "Daniel C.", 
        "id": "sg:person.01106760406.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106760406.68"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada", 
          "id": "http://www.grid.ac/institutes/grid.443931.8", 
          "name": [
            "National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thomas", 
        "givenName": "David Y.", 
        "id": "sg:person.07505723122.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07505723122.58"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1996-01-22", 
    "datePublishedReg": "1996-01-22", 
    "description": "We present in this chapter a polymerase chain reaction (PCR)-based method to simultaneously introduce and remove large fragments of DNA in a single mutagenesis reaction without the need for restriction sites. We have favored the use of long single-stranded DNA primers synthesized by asymmetric PCR (1,2), whereas others have used double-stranded PCR-amplified fragment directly (3\u20135) as primers in in vitro mutagenesis reactions.", 
    "genre": "chapter", 
    "id": "sg:pub.10.1385/0-89603-332-5:229", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-0-89603-332-0", 
        "978-1-59259-544-0"
      ], 
      "name": "In Vitro Mutagenesis Protocols", 
      "type": "Book"
    }, 
    "keywords": [
      "polymerase chain reaction", 
      "chain reaction", 
      "PCR", 
      "insertions/deletions", 
      "deletion", 
      "primers", 
      "fragments", 
      "need", 
      "use", 
      "DNA primers", 
      "segments", 
      "reaction", 
      "large fragments", 
      "DNA", 
      "restriction sites", 
      "sites", 
      "mutagenesis reaction", 
      "method", 
      "mutagenesis", 
      "chapter", 
      "DNA segments", 
      "asymmetric PCR", 
      "large DNA segments"
    ], 
    "name": "PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments", 
    "pagination": "229-237", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009409637"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1385/0-89603-332-5:229"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1385/0-89603-332-5:229", 
      "https://app.dimensions.ai/details/publication/pub.1009409637"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-11-24T21:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/chapter/chapter_141.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1385/0-89603-332-5:229"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1385/0-89603-332-5:229'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1385/0-89603-332-5:229'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1385/0-89603-332-5:229'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1385/0-89603-332-5:229'


 

This table displays all metadata directly associated to this object as RDF triples.

83 TRIPLES      21 PREDICATES      45 URIs      38 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1385/0-89603-332-5:229 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nad874ccd5914401c8d1f2fb929787b32
4 schema:datePublished 1996-01-22
5 schema:datePublishedReg 1996-01-22
6 schema:description We present in this chapter a polymerase chain reaction (PCR)-based method to simultaneously introduce and remove large fragments of DNA in a single mutagenesis reaction without the need for restriction sites. We have favored the use of long single-stranded DNA primers synthesized by asymmetric PCR (1,2), whereas others have used double-stranded PCR-amplified fragment directly (3–5) as primers in in vitro mutagenesis reactions.
7 schema:genre chapter
8 schema:isAccessibleForFree false
9 schema:isPartOf N22e4b1baa896499db39a4533a2c63c8d
10 schema:keywords DNA
11 DNA primers
12 DNA segments
13 PCR
14 asymmetric PCR
15 chain reaction
16 chapter
17 deletion
18 fragments
19 insertions/deletions
20 large DNA segments
21 large fragments
22 method
23 mutagenesis
24 mutagenesis reaction
25 need
26 polymerase chain reaction
27 primers
28 reaction
29 restriction sites
30 segments
31 sites
32 use
33 schema:name PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments
34 schema:pagination 229-237
35 schema:productId Nc758611848a74297a7a607a3addfd09f
36 Nd34a534e73624ade88a561f4192055d5
37 schema:publisher N23d1fe73e8304eceba9d0340d88c0618
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009409637
39 https://doi.org/10.1385/0-89603-332-5:229
40 schema:sdDatePublished 2022-11-24T21:12
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher Ncde7da58ebac4235b5bd99d99c46c1e5
43 schema:url https://doi.org/10.1385/0-89603-332-5:229
44 sgo:license sg:explorer/license/
45 sgo:sdDataset chapters
46 rdf:type schema:Chapter
47 N22e4b1baa896499db39a4533a2c63c8d schema:isbn 978-0-89603-332-0
48 978-1-59259-544-0
49 schema:name In Vitro Mutagenesis Protocols
50 rdf:type schema:Book
51 N23d1fe73e8304eceba9d0340d88c0618 schema:name Springer Nature
52 rdf:type schema:Organisation
53 N6fd2a925dee340358428944c8d5270f8 rdf:first sg:person.07505723122.58
54 rdf:rest rdf:nil
55 Nad874ccd5914401c8d1f2fb929787b32 rdf:first sg:person.01106760406.68
56 rdf:rest N6fd2a925dee340358428944c8d5270f8
57 Nc758611848a74297a7a607a3addfd09f schema:name dimensions_id
58 schema:value pub.1009409637
59 rdf:type schema:PropertyValue
60 Ncde7da58ebac4235b5bd99d99c46c1e5 schema:name Springer Nature - SN SciGraph project
61 rdf:type schema:Organization
62 Nd34a534e73624ade88a561f4192055d5 schema:name doi
63 schema:value 10.1385/0-89603-332-5:229
64 rdf:type schema:PropertyValue
65 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
66 schema:name Biological Sciences
67 rdf:type schema:DefinedTerm
68 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
69 schema:name Genetics
70 rdf:type schema:DefinedTerm
71 sg:person.01106760406.68 schema:affiliation grid-institutes:grid.443931.8
72 schema:familyName Tessier
73 schema:givenName Daniel C.
74 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106760406.68
75 rdf:type schema:Person
76 sg:person.07505723122.58 schema:affiliation grid-institutes:grid.443931.8
77 schema:familyName Thomas
78 schema:givenName David Y.
79 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07505723122.58
80 rdf:type schema:Person
81 grid-institutes:grid.443931.8 schema:alternateName National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada
82 schema:name National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada
83 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...