Ontology type: schema:Chapter
1995-09-27
AUTHORSHeddwyn Jones , Jennifer F. Topping , Wenbin Wei , Michael C. Clarke , Paul Muskett , Keith Lindsey
ABSTRACTA number of methods have been developed for the isolation of plant genes, including the differential screening of cDNA libraries, the use of heterologous probes, and the use of protein sequence data to generate oligonucleotides for library screening. These have been well-reviewed. The advent of plant transformation techniques has further increased the tools available for the functional analysis of isolated genes, such as for the introduction of promoter deletion-reporter gene fusions, for the overexpression of the gene product or for generating null expressors by antisense RNA technology. More recently, transformation techniques have been exploited to develop new approaches to isolate plant genes, and these methods can be considered as being of two types: insertional mutagenesis and gene trapping. More... »
PAGES63-76
Plant Gene Transfer and Expression Protocols
ISBN0-89603-321-X
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DOIhttp://dx.doi.org/10.1385/0-89603-321-x:63
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