Cytometric Detection and Quantitation of Cell–Cell Electrofusion Products View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1995-08-11

AUTHORS

Jac A. Nickoloff , Mark J. Jaroszeski , Richard Gilbert , Richard Heller

ABSTRACT

Cell-cell electrofusion (CCE) is a process that involves forcing cells into close juxtaposition and then inducing fusion by delivering electric pulses to the cells. CCE has proven to have many practical applications. It has been used for monoclonal antibody (MAb) production (1,2), hybridoma production (3–5), and to transfer membrane-surface markers (6). Many other applications are described in this volume. In addition, the study of membrane fusion mechanisms has been the focus of some researchers (7–12). Electrofusion techniques seldom result in 100% yield between fusion partners. Therefore, a major aspect of CCE applications is the ability to detect and quantitate fusion products. More... »

PAGES

355-364

References to SciGraph publications

Book

TITLE

Animal Cell Electroporation and Electrofusion Protocols

ISBN

0-89603-304-X

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/0-89603-304-x:355

DOI

http://dx.doi.org/10.1385/0-89603-304-x:355

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026081385


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