Free Chromatin Mapping by FISH View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1994-10-31

AUTHORS

K. H. Andy Choo , Henry H. Q. Heng , Lap-Chee Tsui

ABSTRACT

Different strategies have been introduced to facilitate characterization of complex genomes; the techniques include molecular cloning, agarose gel electrophoresis and blot-hybridization analysis, DNA sequencing, construction of somatic cell hybrid lines, in situ hybridization, as well as genetic (linkage) analysis. Because of their wide application, numerous improvements have been made in each of these areas in recent years, for example, the use of pulsed-field gel electrophoresis (PFGE), yeast artificial chromosome (YAC), and fluorescence in situ hybridization (FISH) (1–4). Obviously, no single method can provide all the information required for detailed analysis of genomes with greater than several billion base pairs (bp) and each method has its limitation in range of resolution. A combination of different techniques is therefore necessary for any large-scale gene mapping endeavors. More... »

PAGES

109-122

References to SciGraph publications

Book

TITLE

In Situ Hybridization Protocols

ISBN

0-89603-280-9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/0-89603-280-9:109

DOI

http://dx.doi.org/10.1385/0-89603-280-9:109

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021681074


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