Detecting mRNAin Tissue Sections with Digoxigenin-Labeled Probes View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1993-11-29

AUTHORS

Giorgio Terenghi , Julia M. Polak

ABSTRACT

Nonradioactively labeled probes offer several advantages compared to radioactive ones, as they show long stability, high morphological resolution, and rapid developing time. There are different types of nonradioactive labeling methods available, although recently digoxigenin-labeled probes (1) have become the most widely used for investigation on animal tissue, as they offer the advantage of low background noise and increased sensitivity (2,3). Also, digoxigenin can be used to label either RNA, DNA, or oligonucleotide probes. There have been different opinions on the sensitivity of detection of digoxigenin probes, but recent publications have shown that the sensitivity of radiolabeled and nonradioactive probes is comparable (3). More... »

PAGES

193-199

Book

TITLE

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

ISBN

0-89603-254-X

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/0-89603-254-x:193

DOI

http://dx.doi.org/10.1385/0-89603-254-x:193

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053012172


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