Direct Gene Transfer into Plant Protoplasts View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1988-08-31

AUTHORS

John M. Walker , Keith Lindsey , Michael G. K. Jones , Neil Fish

ABSTRACT

The techniques of plant molecular biology have advanced rapidly in the last 5 yr, and, for a number of plant species, including some crops, it is now possible to bypass traditional plant breeding techniques and introduce specific genes directly. The earliest reports of the transformation of plants with foreign genes exploited the fact that the soil bacterium Agrobacterium tumefaciens, which induces the formation of crown galls, transfers part of a plasmid (the tumor-inducing, Ti, plasmid) into its host (e.g., ref. 1). The isolation and modification of this plasmid by the insertion of one or more structural genes together with regulatory elements has provided the means of genetically manipulating intact plants, cultured plant tissues, and protoplasts. More... »

PAGES

519-36

References to SciGraph publications

  • 1985-03. Resistance to hygromycin B in PLANT MOLECULAR BIOLOGY
  • 1986-09. Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree) in PLANT MOLECULAR BIOLOGY
  • 1985-12. High Efficiency Direct Gene Transfer to Plants in NATURE BIOTECHNOLOGY
  • 1975-12. Effect of electric fields on light-scattering and fluorescence of chromaffin granules in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1987-11. The permeability of electroporated cells and protoplasts of sugar beet in PLANTA
  • 1985-05. Direct gene transfer to cells of a graminaceous monocot in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Gene transfer to cereal cells mediated by protoplast transformation in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1985-09. A chimaeric hygromycin resistance gene as a selectable marker in plant cells in PLANT MOLECULAR BIOLOGY
  • 1972-12. Permeability changes induced by electric impulses in vesicular membranes in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1983-05. Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector in NATURE
  • 1986-02. Stable transformation of maize after gene transfer by electroporation in NATURE
  • 1984-10. Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens in NATURE
  • 1982-03. In vitro transformation of plant protoplasts with Ti-plasmid DNA in NATURE
  • 1977-12. Kinetics of permeability changes induced by electric impulses in chromaffin granules in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1985-12. Electric field mediated stable transformation of carrot protoplasts with naked DNA in PLANT CELL REPORTS
  • 1986-07. Changes in translatable poly(A) RNA from differentiated potato tissues transformed with shoot-inducing Ti TL-DNA of Agrobacterium tumefaciens in PLANT MOLECULAR BIOLOGY
  • 1980-10. Phospholipid composition and fatty acid desaturation in the roots of rye during acclimatization of low temperature in PLANTA
  • 1974-10. Size and location of the transforming region in human adenovirus type 5 DNA in NATURE
  • 1986-03. Transient gene expression in a Gramineae cell line in MOLECULAR GENETICS AND GENOMICS
  • 1974-06. A procedure for the in vivo determination of enzyme activity in higher plant tissue in PLANTA
  • 1985-07. Expression of shoot-inducing Ti TL-DNA in differentiated tissues of potato (Solanum tuberosum cv Maris Bard) in PLANT MOLECULAR BIOLOGY
  • Book

    TITLE

    New Nucleic Acid Techniques

    ISBN

    0-89603-127-6

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1385/0-89603-127-6:519

    DOI

    http://dx.doi.org/10.1385/0-89603-127-6:519

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011379543

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21424662


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