Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-Forming Derivatization and LC with Fluorescence Detection View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-08

AUTHORS

Fumio Ichinose, Takashi Yoshitake, Hideyuki Yoshida, Kenichiro Todoroki, Jan Kehr, Osamu Inoue, Hitoshi Nohta, Masatoshi Yamaguchi

ABSTRACT

A highly sensitive and selective method for determination of histamine in rat plasma and tissue extracts by liquid chromatography with fluorescence detection is described. The method is based on precolumn derivatization of amino groups of histamine with two molecules of 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester that allows generation of intramolecular excimer fluorescence. The detection limit for histamine was 0.183 nM. This sensitivity allowed determination of histamine in 10 μL of rat plasma or in the extracts from less than 1 mg of tissue. More... »

PAGES

575-580

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1365/s10337-009-1162-4

DOI

http://dx.doi.org/10.1365/s10337-009-1162-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006491964


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