Quantitative TLC Analysis of Sterol (24β-Ethylcholesta-5,22E,25-triene-3β-ol) in Agnimantha (Clerodendrum phlomidis Linn) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-01-05

AUTHORS

Karuna Shanker, Subhash Chandra Singh, Sangita Pant, Pooja Srivastava, Akhilesh Kumar Yadav, Richa Pandey, Ram Kishor Verma, Madan Mohan Gupta

ABSTRACT

A quantitative method using silica gel 60F254 high performance thin layer chromatography plates, automated bandwise sample application, and automated visible mode densitometric method has been developed for the determination of 24β-ethylcholesta-5,22E,25-triene-3β-ol (ECTO) in the aerial part of Clerodendrum phlomidis. ECTO was used as a chemical marker for the standardization of C. phlomidis plant extracts. The separation was performed on silica gel 60F254 TLC plates using chloroform-methanol (98.5: 1.5, v/v) as mobile phase. The quantitation of ECTO was carried out using the densitometric reflection/absorption mode at 650 nm after post chromatographic derivatization with anisaldehyde reagent. A precise and accurate quantification can be performed in the linear working concentration range of 150–400 ng band−1 with good correlation (r2 = 0.996). The method was validated for peak purities, precision, robustness, limit of detection (LOD) and quantitation (LOQ), etc. as per ICH guidelines. More... »

PAGES

269-274

Identifiers

URI

http://scigraph.springernature.com/pub.10.1365/s10337-007-0502-5

DOI

http://dx.doi.org/10.1365/s10337-007-0502-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032445782


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