Quantitative TLC Analysis of Sterol (24β-Ethylcholesta-5,22E,25-triene-3β-ol) in Agnimantha (Clerodendrum phlomidis Linn) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-01-05

AUTHORS

Karuna Shanker, Subhash Chandra Singh, Sangita Pant, Pooja Srivastava, Akhilesh Kumar Yadav, Richa Pandey, Ram Kishor Verma, Madan Mohan Gupta

ABSTRACT

A quantitative method using silica gel 60F254 high performance thin layer chromatography plates, automated bandwise sample application, and automated visible mode densitometric method has been developed for the determination of 24β-ethylcholesta-5,22E,25-triene-3β-ol (ECTO) in the aerial part of Clerodendrum phlomidis. ECTO was used as a chemical marker for the standardization of C. phlomidis plant extracts. The separation was performed on silica gel 60F254 TLC plates using chloroform-methanol (98.5: 1.5, v/v) as mobile phase. The quantitation of ECTO was carried out using the densitometric reflection/absorption mode at 650 nm after post chromatographic derivatization with anisaldehyde reagent. A precise and accurate quantification can be performed in the linear working concentration range of 150–400 ng band−1 with good correlation (r2 = 0.996). The method was validated for peak purities, precision, robustness, limit of detection (LOD) and quantitation (LOQ), etc. as per ICH guidelines. More... »

PAGES

269-274

Identifiers

URI

http://scigraph.springernature.com/pub.10.1365/s10337-007-0502-5

DOI

http://dx.doi.org/10.1365/s10337-007-0502-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032445782


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Analytical Chemistry", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shanker", 
        "givenName": "Karuna", 
        "id": "sg:person.0650767015.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650767015.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Botany and Pharmacognosy, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Department of Botany and Pharmacognosy, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Singh", 
        "givenName": "Subhash Chandra", 
        "id": "sg:person.016707760464.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016707760464.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pant", 
        "givenName": "Sangita", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Srivastava", 
        "givenName": "Pooja", 
        "id": "sg:person.01030066455.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030066455.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yadav", 
        "givenName": "Akhilesh Kumar", 
        "id": "sg:person.0713640055.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713640055.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pandey", 
        "givenName": "Richa", 
        "id": "sg:person.01327465226.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327465226.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Verma", 
        "givenName": "Ram Kishor", 
        "id": "sg:person.01316361674.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316361674.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gupta", 
        "givenName": "Madan Mohan", 
        "id": "sg:person.01242460441.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2008-01-05", 
    "datePublishedReg": "2008-01-05", 
    "description": "A quantitative method using silica gel 60F254 high performance thin layer chromatography plates, automated bandwise sample application, and automated visible mode densitometric method has been developed for the determination of 24\u03b2-ethylcholesta-5,22E,25-triene-3\u03b2-ol (ECTO) in the aerial part of Clerodendrum phlomidis. ECTO was used as a chemical marker for the standardization of C. phlomidis plant extracts. The separation was performed on silica gel 60F254 TLC plates using chloroform-methanol (98.5: 1.5, v/v) as mobile phase. The quantitation of ECTO was carried out using the densitometric reflection/absorption mode at 650\u00a0nm after post chromatographic derivatization with anisaldehyde reagent. A precise and accurate quantification can be performed in the linear working concentration range of 150\u2013400\u00a0ng\u00a0band\u22121 with good correlation (r2\u00a0=\u00a00.996). The method was validated for peak purities, precision, robustness, limit of detection (LOD) and quantitation (LOQ), etc. as per ICH guidelines.", 
    "genre": "article", 
    "id": "sg:pub.10.1365/s10337-007-0502-5", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1015080", 
        "issn": [
          "0009-5893", 
          "1612-1112"
        ], 
        "name": "Chromatographia", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3-4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "67"
      }
    ], 
    "keywords": [
      "densitometric reflection/absorption mode", 
      "limit of detection", 
      "reflection/absorption mode", 
      "linear working concentration range", 
      "silica gel 60F254 TLC plates", 
      "post-chromatographic derivatization", 
      "working concentration range", 
      "high-performance thin-layer chromatography plates", 
      "quantitative TLC analysis", 
      "anisaldehyde reagent", 
      "Clerodendrum phlomidis", 
      "absorption mode", 
      "concentration range", 
      "plant extracts", 
      "densitometric method", 
      "peak purity", 
      "sample application", 
      "ICH guidelines", 
      "mobile phase", 
      "accurate quantification", 
      "TLC plates", 
      "phlomidis", 
      "chromatography plates", 
      "quantitation", 
      "thin-layer chromatography plates", 
      "applications", 
      "purity", 
      "Agnimantha", 
      "detection", 
      "method", 
      "TLC analysis", 
      "limit", 
      "reagents", 
      "good correlation", 
      "separation", 
      "range", 
      "mode", 
      "quantification", 
      "robustness", 
      "derivatization", 
      "precision", 
      "plate", 
      "extract", 
      "standardization", 
      "phase", 
      "determination", 
      "quantitative methods", 
      "analysis", 
      "chemical markers", 
      "sterols", 
      "part", 
      "markers", 
      "correlation", 
      "aerial parts", 
      "ol", 
      "ecto", 
      "guidelines"
    ], 
    "name": "Quantitative TLC Analysis of Sterol (24\u03b2-Ethylcholesta-5,22E,25-triene-3\u03b2-ol) in Agnimantha (Clerodendrum phlomidis Linn)", 
    "pagination": "269-274", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1032445782"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1365/s10337-007-0502-5"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1365/s10337-007-0502-5", 
      "https://app.dimensions.ai/details/publication/pub.1032445782"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:24", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_462.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1365/s10337-007-0502-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1365/s10337-007-0502-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1365/s10337-007-0502-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1365/s10337-007-0502-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1365/s10337-007-0502-5'


 

This table displays all metadata directly associated to this object as RDF triples.

165 TRIPLES      21 PREDICATES      82 URIs      74 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1365/s10337-007-0502-5 schema:about anzsrc-for:03
2 anzsrc-for:0301
3 schema:author Nee74dbd9c807426cbc7faae1f777ea7c
4 schema:datePublished 2008-01-05
5 schema:datePublishedReg 2008-01-05
6 schema:description A quantitative method using silica gel 60F254 high performance thin layer chromatography plates, automated bandwise sample application, and automated visible mode densitometric method has been developed for the determination of 24β-ethylcholesta-5,22E,25-triene-3β-ol (ECTO) in the aerial part of Clerodendrum phlomidis. ECTO was used as a chemical marker for the standardization of C. phlomidis plant extracts. The separation was performed on silica gel 60F254 TLC plates using chloroform-methanol (98.5: 1.5, v/v) as mobile phase. The quantitation of ECTO was carried out using the densitometric reflection/absorption mode at 650 nm after post chromatographic derivatization with anisaldehyde reagent. A precise and accurate quantification can be performed in the linear working concentration range of 150–400 ng band−1 with good correlation (r2 = 0.996). The method was validated for peak purities, precision, robustness, limit of detection (LOD) and quantitation (LOQ), etc. as per ICH guidelines.
7 schema:genre article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N22ec16bd01d5488d9b5a519088988bb8
11 N49920d610b784dceac04ade81b73edd1
12 sg:journal.1015080
13 schema:keywords Agnimantha
14 Clerodendrum phlomidis
15 ICH guidelines
16 TLC analysis
17 TLC plates
18 absorption mode
19 accurate quantification
20 aerial parts
21 analysis
22 anisaldehyde reagent
23 applications
24 chemical markers
25 chromatography plates
26 concentration range
27 correlation
28 densitometric method
29 densitometric reflection/absorption mode
30 derivatization
31 detection
32 determination
33 ecto
34 extract
35 good correlation
36 guidelines
37 high-performance thin-layer chromatography plates
38 limit
39 limit of detection
40 linear working concentration range
41 markers
42 method
43 mobile phase
44 mode
45 ol
46 part
47 peak purity
48 phase
49 phlomidis
50 plant extracts
51 plate
52 post-chromatographic derivatization
53 precision
54 purity
55 quantification
56 quantitation
57 quantitative TLC analysis
58 quantitative methods
59 range
60 reagents
61 reflection/absorption mode
62 robustness
63 sample application
64 separation
65 silica gel 60F254 TLC plates
66 standardization
67 sterols
68 thin-layer chromatography plates
69 working concentration range
70 schema:name Quantitative TLC Analysis of Sterol (24β-Ethylcholesta-5,22E,25-triene-3β-ol) in Agnimantha (Clerodendrum phlomidis Linn)
71 schema:pagination 269-274
72 schema:productId N060570baaa5844f8a738dfc63d2a0ee0
73 N1765c1266bba40e0ae88b78bcdc5cfe3
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032445782
75 https://doi.org/10.1365/s10337-007-0502-5
76 schema:sdDatePublished 2022-05-20T07:24
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher N46811becaf3446c38cddb7f9ddca774f
79 schema:url https://doi.org/10.1365/s10337-007-0502-5
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N05e6d83a4c6445b1b9ccc7207b381fec rdf:first sg:person.0713640055.22
84 rdf:rest Nab03acc05ae2438fab62d476522d99bd
85 N060570baaa5844f8a738dfc63d2a0ee0 schema:name dimensions_id
86 schema:value pub.1032445782
87 rdf:type schema:PropertyValue
88 N1765c1266bba40e0ae88b78bcdc5cfe3 schema:name doi
89 schema:value 10.1365/s10337-007-0502-5
90 rdf:type schema:PropertyValue
91 N22ec16bd01d5488d9b5a519088988bb8 schema:volumeNumber 67
92 rdf:type schema:PublicationVolume
93 N37c4574de93a4ffc9a739b5a9eb71e21 rdf:first sg:person.016707760464.06
94 rdf:rest Nbfcf532b1bbe4611a164fb1ab6aeee26
95 N46811becaf3446c38cddb7f9ddca774f schema:name Springer Nature - SN SciGraph project
96 rdf:type schema:Organization
97 N49920d610b784dceac04ade81b73edd1 schema:issueNumber 3-4
98 rdf:type schema:PublicationIssue
99 N9ac5b620f96449898e45429c86d8905a schema:affiliation grid-institutes:grid.417631.6
100 schema:familyName Pant
101 schema:givenName Sangita
102 rdf:type schema:Person
103 Nab03acc05ae2438fab62d476522d99bd rdf:first sg:person.01327465226.39
104 rdf:rest Ne95f2ddd04b8483d8fe3e992341182db
105 Nab6332f266f34f93ba045497ba694d67 rdf:first sg:person.01242460441.07
106 rdf:rest rdf:nil
107 Nbfcf532b1bbe4611a164fb1ab6aeee26 rdf:first N9ac5b620f96449898e45429c86d8905a
108 rdf:rest Nc55cd0acc88a42048cc94d66f416bd02
109 Nc55cd0acc88a42048cc94d66f416bd02 rdf:first sg:person.01030066455.62
110 rdf:rest N05e6d83a4c6445b1b9ccc7207b381fec
111 Ne95f2ddd04b8483d8fe3e992341182db rdf:first sg:person.01316361674.98
112 rdf:rest Nab6332f266f34f93ba045497ba694d67
113 Nee74dbd9c807426cbc7faae1f777ea7c rdf:first sg:person.0650767015.23
114 rdf:rest N37c4574de93a4ffc9a739b5a9eb71e21
115 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
116 schema:name Chemical Sciences
117 rdf:type schema:DefinedTerm
118 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
119 schema:name Analytical Chemistry
120 rdf:type schema:DefinedTerm
121 sg:journal.1015080 schema:issn 0009-5893
122 1612-1112
123 schema:name Chromatographia
124 schema:publisher Springer Nature
125 rdf:type schema:Periodical
126 sg:person.01030066455.62 schema:affiliation grid-institutes:grid.417631.6
127 schema:familyName Srivastava
128 schema:givenName Pooja
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030066455.62
130 rdf:type schema:Person
131 sg:person.01242460441.07 schema:affiliation grid-institutes:grid.417631.6
132 schema:familyName Gupta
133 schema:givenName Madan Mohan
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07
135 rdf:type schema:Person
136 sg:person.01316361674.98 schema:affiliation grid-institutes:grid.417631.6
137 schema:familyName Verma
138 schema:givenName Ram Kishor
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316361674.98
140 rdf:type schema:Person
141 sg:person.01327465226.39 schema:affiliation grid-institutes:grid.417631.6
142 schema:familyName Pandey
143 schema:givenName Richa
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327465226.39
145 rdf:type schema:Person
146 sg:person.016707760464.06 schema:affiliation grid-institutes:grid.417631.6
147 schema:familyName Singh
148 schema:givenName Subhash Chandra
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016707760464.06
150 rdf:type schema:Person
151 sg:person.0650767015.23 schema:affiliation grid-institutes:grid.417631.6
152 schema:familyName Shanker
153 schema:givenName Karuna
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650767015.23
155 rdf:type schema:Person
156 sg:person.0713640055.22 schema:affiliation grid-institutes:grid.417631.6
157 schema:familyName Yadav
158 schema:givenName Akhilesh Kumar
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713640055.22
160 rdf:type schema:Person
161 grid-institutes:grid.417631.6 schema:alternateName Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India
162 Department of Botany and Pharmacognosy, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India
163 schema:name Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India
164 Department of Botany and Pharmacognosy, Central Institute of Medicinal and Aromatic Plants, 226015, Lucknow, India
165 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...