Introducing antisense waxy gene into rice seeds reduces grain amylose contents using a safe transgenic technique View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-01

AUTHORS

Jianyue Li, Wanxia Mao, Lijun Yang, Genyu Zhou, Jia Liu, Qitao Yan, Dong Mi

ABSTRACT

An agrobacterium tumefaciensmediated co-transformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vector pl3W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other binary vector pl3W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing pl3W4 and pl3W8 mixed at a ratio of 1: 9, were used to transform a high-yield rice strain Chao 2–10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1 seeds with GUS positive were eliminated. The GUS negative T1 seeds were germinated, and the seedlings were analyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were confirmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice resource for eating quality improvement. More... »

PAGES

39-44

Identifiers

URI

http://scigraph.springernature.com/pub.10.1360/982004-378

DOI

http://dx.doi.org/10.1360/982004-378

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1065071707


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Jianyue", 
        "id": "sg:person.01320147414.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320147414.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China", 
            "Shanghai Zhongshan School, 200023, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mao", 
        "givenName": "Wanxia", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Lijun", 
        "id": "sg:person.01252034214.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252034214.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Genyu", 
        "id": "sg:person.01205525645.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205525645.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Jia", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yan", 
        "givenName": "Qitao", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Normal University", 
          "id": "https://www.grid.ac/institutes/grid.412531.0", 
          "name": [
            "Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mi", 
        "givenName": "Dong", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2003.10.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003623577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.pp.46.060195.002355", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008037242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006184221051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029358994", 
          "https://doi.org/10.1023/a:1006184221051"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.6.2117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032218295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/pcp/pcd008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032759539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00222654", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033374626", 
          "https://doi.org/10.1007/bf00222654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02907608", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037276768", 
          "https://doi.org/10.1007/bf02907608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02907608", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037276768", 
          "https://doi.org/10.1007/bf02907608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1996.10010165.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038680141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1022148824018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047485483", 
          "https://doi.org/10.1023/a:1022148824018"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-01", 
    "datePublishedReg": "2005-01-01", 
    "description": "An agrobacterium tumefaciensmediated co-transformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vector pl3W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other binary vector pl3W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing pl3W4 and pl3W8 mixed at a ratio of 1: 9, were used to transform a high-yield rice strain Chao 2\u201310. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1 seeds with GUS positive were eliminated. The GUS negative T1 seeds were germinated, and the seedlings were analyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were confirmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice resource for eating quality improvement.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1360/982004-378", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1051679", 
        "issn": [
          "2095-9273", 
          "2095-9281"
        ], 
        "name": "Science Bulletin", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "50"
      }
    ], 
    "name": "Introducing antisense waxy gene into rice seeds reduces grain amylose contents using a safe transgenic technique", 
    "pagination": "39-44", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9e6baf0ed967877dc68eec498432f0c4b06f6c559bd983107658a1f1abb78914"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1360/982004-378"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1065071707"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1360/982004-378", 
      "https://app.dimensions.ai/details/publication/pub.1065071707"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000501.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1360/982004-378"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1360/982004-378'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1360/982004-378'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1360/982004-378'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1360/982004-378'


 

This table displays all metadata directly associated to this object as RDF triples.

131 TRIPLES      21 PREDICATES      36 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1360/982004-378 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 schema:author Na153f25abd8b46048392f100ca71f14c
4 schema:citation sg:pub.10.1007/bf00222654
5 sg:pub.10.1007/bf02907608
6 sg:pub.10.1023/a:1006184221051
7 sg:pub.10.1023/a:1022148824018
8 https://doi.org/10.1016/j.jbiotec.2003.10.011
9 https://doi.org/10.1046/j.1365-313x.1996.10010165.x
10 https://doi.org/10.1073/pnas.94.6.2117
11 https://doi.org/10.1093/pcp/pcd008
12 https://doi.org/10.1146/annurev.pp.46.060195.002355
13 schema:datePublished 2005-01
14 schema:datePublishedReg 2005-01-01
15 schema:description An agrobacterium tumefaciensmediated co-transformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vector pl3W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other binary vector pl3W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing pl3W4 and pl3W8 mixed at a ratio of 1: 9, were used to transform a high-yield rice strain Chao 2–10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1 seeds with GUS positive were eliminated. The GUS negative T1 seeds were germinated, and the seedlings were analyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were confirmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice resource for eating quality improvement.
16 schema:genre research_article
17 schema:inLanguage en
18 schema:isAccessibleForFree false
19 schema:isPartOf N1e41d6ce8de744758376c5b9103fd832
20 N7059cc05f472439bae807b171adf55fd
21 sg:journal.1051679
22 schema:name Introducing antisense waxy gene into rice seeds reduces grain amylose contents using a safe transgenic technique
23 schema:pagination 39-44
24 schema:productId N07c6f1d304ae4aeea263586497f4251c
25 Na6f0b49c749c4b678cf016817d34f390
26 Nd6448e79a9a945e09adfe1e5dbb1fa69
27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065071707
28 https://doi.org/10.1360/982004-378
29 schema:sdDatePublished 2019-04-10T15:49
30 schema:sdLicense https://scigraph.springernature.com/explorer/license/
31 schema:sdPublisher Nac3b6ec6f4bf4e73bfb1cd4471008877
32 schema:url http://link.springer.com/10.1360/982004-378
33 sgo:license sg:explorer/license/
34 sgo:sdDataset articles
35 rdf:type schema:ScholarlyArticle
36 N07c6f1d304ae4aeea263586497f4251c schema:name doi
37 schema:value 10.1360/982004-378
38 rdf:type schema:PropertyValue
39 N178c6deec2c34bf980f00e02f32fdb14 rdf:first sg:person.01205525645.21
40 rdf:rest N36ab93028fa84e3982668ebabe162b8e
41 N1e41d6ce8de744758376c5b9103fd832 schema:issueNumber 1
42 rdf:type schema:PublicationIssue
43 N327b2d256785434fa65a346eb621542b schema:affiliation https://www.grid.ac/institutes/grid.412531.0
44 schema:familyName Mao
45 schema:givenName Wanxia
46 rdf:type schema:Person
47 N36ab93028fa84e3982668ebabe162b8e rdf:first N40d606c564e14980b26cf1884a292d1e
48 rdf:rest Ne82d2358371845caab043e72829a3d40
49 N40d606c564e14980b26cf1884a292d1e schema:affiliation https://www.grid.ac/institutes/grid.412531.0
50 schema:familyName Liu
51 schema:givenName Jia
52 rdf:type schema:Person
53 N7059cc05f472439bae807b171adf55fd schema:volumeNumber 50
54 rdf:type schema:PublicationVolume
55 N857a68db5684453f858c144403dcef81 schema:affiliation https://www.grid.ac/institutes/grid.412531.0
56 schema:familyName Mi
57 schema:givenName Dong
58 rdf:type schema:Person
59 Na153f25abd8b46048392f100ca71f14c rdf:first sg:person.01320147414.12
60 rdf:rest Nc977707bec2b44e6ab8cd815647112af
61 Na564d1c72d0e4101b9e863621a944bf7 schema:affiliation https://www.grid.ac/institutes/grid.412531.0
62 schema:familyName Yan
63 schema:givenName Qitao
64 rdf:type schema:Person
65 Na6f0b49c749c4b678cf016817d34f390 schema:name dimensions_id
66 schema:value pub.1065071707
67 rdf:type schema:PropertyValue
68 Nac3b6ec6f4bf4e73bfb1cd4471008877 schema:name Springer Nature - SN SciGraph project
69 rdf:type schema:Organization
70 Nc977707bec2b44e6ab8cd815647112af rdf:first N327b2d256785434fa65a346eb621542b
71 rdf:rest Ndb9cd7a872a44047a26cb75ef6ae76bf
72 Nca9be40b484341f3b8831847280760f2 rdf:first N857a68db5684453f858c144403dcef81
73 rdf:rest rdf:nil
74 Nd6448e79a9a945e09adfe1e5dbb1fa69 schema:name readcube_id
75 schema:value 9e6baf0ed967877dc68eec498432f0c4b06f6c559bd983107658a1f1abb78914
76 rdf:type schema:PropertyValue
77 Ndb9cd7a872a44047a26cb75ef6ae76bf rdf:first sg:person.01252034214.07
78 rdf:rest N178c6deec2c34bf980f00e02f32fdb14
79 Ne82d2358371845caab043e72829a3d40 rdf:first Na564d1c72d0e4101b9e863621a944bf7
80 rdf:rest Nca9be40b484341f3b8831847280760f2
81 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
82 schema:name Biological Sciences
83 rdf:type schema:DefinedTerm
84 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
85 schema:name Plant Biology
86 rdf:type schema:DefinedTerm
87 sg:journal.1051679 schema:issn 2095-9273
88 2095-9281
89 schema:name Science Bulletin
90 rdf:type schema:Periodical
91 sg:person.01205525645.21 schema:affiliation https://www.grid.ac/institutes/grid.412531.0
92 schema:familyName Zhou
93 schema:givenName Genyu
94 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205525645.21
95 rdf:type schema:Person
96 sg:person.01252034214.07 schema:affiliation https://www.grid.ac/institutes/grid.412531.0
97 schema:familyName Yang
98 schema:givenName Lijun
99 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252034214.07
100 rdf:type schema:Person
101 sg:person.01320147414.12 schema:affiliation https://www.grid.ac/institutes/grid.412531.0
102 schema:familyName Li
103 schema:givenName Jianyue
104 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320147414.12
105 rdf:type schema:Person
106 sg:pub.10.1007/bf00222654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033374626
107 https://doi.org/10.1007/bf00222654
108 rdf:type schema:CreativeWork
109 sg:pub.10.1007/bf02907608 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037276768
110 https://doi.org/10.1007/bf02907608
111 rdf:type schema:CreativeWork
112 sg:pub.10.1023/a:1006184221051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029358994
113 https://doi.org/10.1023/a:1006184221051
114 rdf:type schema:CreativeWork
115 sg:pub.10.1023/a:1022148824018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047485483
116 https://doi.org/10.1023/a:1022148824018
117 rdf:type schema:CreativeWork
118 https://doi.org/10.1016/j.jbiotec.2003.10.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003623577
119 rdf:type schema:CreativeWork
120 https://doi.org/10.1046/j.1365-313x.1996.10010165.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038680141
121 rdf:type schema:CreativeWork
122 https://doi.org/10.1073/pnas.94.6.2117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032218295
123 rdf:type schema:CreativeWork
124 https://doi.org/10.1093/pcp/pcd008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032759539
125 rdf:type schema:CreativeWork
126 https://doi.org/10.1146/annurev.pp.46.060195.002355 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008037242
127 rdf:type schema:CreativeWork
128 https://www.grid.ac/institutes/grid.412531.0 schema:alternateName Shanghai Normal University
129 schema:name Institute of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, China
130 Shanghai Zhongshan School, 200023, Shanghai, China
131 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...