Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2004-01

AUTHORS

Shirin Munir, Sushmita Singh, Kuljeet Kaur, Vivek Kapur

ABSTRACT

High throughput detection of differential expression of genes is an efficient means of identifying genes and pathways that may play a role in biological systems under certain experimental conditions. There exist a variety of approaches that could be used to identify groups of genes that change in expression in response to a particular stimulus or environment. We here describe the application of suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with a paramyxovirus. SSH was used for initial isolation of differentially expressed transcripts, a large-scale validation of which was accomplished by microarray analysis. The data reveals a large group of regulated genes constituting many biochemical pathways that could serve as targets for future investigations to explore their role in paramyxovirus pathogenesis. The detailed methods described herein could be useful and adaptable to any biological system for studying changes in gene expression. More... »

PAGES

94-104

Journal

TITLE

Biological Procedures Online

ISSUE

1

VOLUME

6

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1251/bpo77

DOI

http://dx.doi.org/10.1251/bpo77

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002263733

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15181476


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