Dealing with different methods for Kluyveromyces lactis β-galactosidase purification View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1998-05

AUTHORS

M. Becerra, E. Cerdán, M. I. González Siso

ABSTRACT

Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme. More... »

PAGES

48-58

Identifiers

URI

http://scigraph.springernature.com/pub.10.1251/bpo4

DOI

http://dx.doi.org/10.1251/bpo4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018977940

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12734592


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00cda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Becerra", 
        "givenName": "M.", 
        "id": "sg:person.01035705111.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035705111.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00cda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cerd\u00e1n", 
        "givenName": "E.", 
        "id": "sg:person.0601204117.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601204117.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00cda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gonz\u00e1lez Siso", 
        "givenName": "M. I.", 
        "id": "sg:person.011245364025.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf02439313", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001623623", 
          "https://doi.org/10.1007/bf02439313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02439313", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001623623", 
          "https://doi.org/10.1007/bf02439313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0032-9592(94)80019-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005877667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0960-8524(96)00036-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011169947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(95)00180-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011398016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-4165(97)00048-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011544794"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3024.1993.tb00607.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013952243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(83)01013-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019090072"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0032-9592(94)80058-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025269696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(94)90171-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033642073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(94)90171-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033642073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0032-9592(94)80053-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040373435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1008885827560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044428070", 
          "https://doi.org/10.1023/a:1008885827560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.1150060905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045786598"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.1150060905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045786598"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(94)00115-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048303666"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00207713-35-1-103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060347883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079679298", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080676369", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081619007", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998-05", 
    "datePublishedReg": "1998-05-01", 
    "description": "Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1251/bpo4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1023701", 
        "issn": [
          "1480-9222"
        ], 
        "name": "Biological Procedures Online", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "1"
      }
    ], 
    "name": "Dealing with different methods for Kluyveromyces lactis \u03b2-galactosidase purification", 
    "pagination": "48-58", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5a6c57b5b7a22bacdc187ff928966f18a0a0dd766dd258d0c17e302519a11f0c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12734592"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100963717"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1251/bpo4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1018977940"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1251/bpo4", 
      "https://app.dimensions.ai/details/publication/pub.1018977940"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89826_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1251%2Fbpo4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1251/bpo4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1251/bpo4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1251/bpo4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1251/bpo4'


 

This table displays all metadata directly associated to this object as RDF triples.

142 TRIPLES      21 PREDICATES      48 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1251/bpo4 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N78298ccc53ef4143ad88a8074b98693a
4 schema:citation sg:pub.10.1007/bf02439313
5 sg:pub.10.1023/a:1008885827560
6 sg:pub.10.1038/227680a0
7 https://app.dimensions.ai/details/publication/pub.1079679298
8 https://app.dimensions.ai/details/publication/pub.1080676369
9 https://app.dimensions.ai/details/publication/pub.1081619007
10 https://doi.org/10.1002/elps.1150060905
11 https://doi.org/10.1016/0003-2697(76)90527-3
12 https://doi.org/10.1016/0032-9592(94)80019-7
13 https://doi.org/10.1016/0032-9592(94)80053-7
14 https://doi.org/10.1016/0032-9592(94)80058-8
15 https://doi.org/10.1016/0076-6879(83)01013-7
16 https://doi.org/10.1016/0141-0229(94)00115-8
17 https://doi.org/10.1016/0141-0229(94)90171-6
18 https://doi.org/10.1016/0141-0229(95)00180-8
19 https://doi.org/10.1016/0960-8524(96)00036-3
20 https://doi.org/10.1016/s0304-4165(97)00048-2
21 https://doi.org/10.1099/00207713-35-1-103
22 https://doi.org/10.1111/j.1365-3024.1993.tb00607.x
23 schema:datePublished 1998-05
24 schema:datePublishedReg 1998-05-01
25 schema:description Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.
26 schema:genre research_article
27 schema:inLanguage en
28 schema:isAccessibleForFree true
29 schema:isPartOf Nd27acfa68570481387029ca00621b354
30 Ne973377e4445435f90310b20b84fbdf5
31 sg:journal.1023701
32 schema:name Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
33 schema:pagination 48-58
34 schema:productId N17bcdab2295f4afb8d4f0aa60a59c11f
35 N24f65845fee6427eaed70be859ce35c7
36 N396bd4e181774896be59b1af53f120d6
37 Nd9bc82d6df3d44ad89726692f365156a
38 Ne56e3800b1f649b28f88357384f8100c
39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018977940
40 https://doi.org/10.1251/bpo4
41 schema:sdDatePublished 2019-04-11T10:04
42 schema:sdLicense https://scigraph.springernature.com/explorer/license/
43 schema:sdPublisher Nf5451877acd043bfad39f2069357ed5a
44 schema:url https://link.springer.com/10.1251%2Fbpo4
45 sgo:license sg:explorer/license/
46 sgo:sdDataset articles
47 rdf:type schema:ScholarlyArticle
48 N0b1be8b073f64c5497b7f75c53a675bc schema:name Departamento de BioloxÍa Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coruña, Spain
49 rdf:type schema:Organization
50 N17bcdab2295f4afb8d4f0aa60a59c11f schema:name doi
51 schema:value 10.1251/bpo4
52 rdf:type schema:PropertyValue
53 N24f65845fee6427eaed70be859ce35c7 schema:name dimensions_id
54 schema:value pub.1018977940
55 rdf:type schema:PropertyValue
56 N396bd4e181774896be59b1af53f120d6 schema:name nlm_unique_id
57 schema:value 100963717
58 rdf:type schema:PropertyValue
59 N4fdc9dfbbdf14105a17303d46a2ca50f schema:name Departamento de BioloxÍa Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coruña, Spain
60 rdf:type schema:Organization
61 N75fee891d3e145209dd28a4df1a24c51 rdf:first sg:person.011245364025.32
62 rdf:rest rdf:nil
63 N78298ccc53ef4143ad88a8074b98693a rdf:first sg:person.01035705111.71
64 rdf:rest Na180d7aa08954d80b09ee67498b2f820
65 Na180d7aa08954d80b09ee67498b2f820 rdf:first sg:person.0601204117.18
66 rdf:rest N75fee891d3e145209dd28a4df1a24c51
67 Na81a91308dff42d589e69f13064a7528 schema:name Departamento de BioloxÍa Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coruña, Spain
68 rdf:type schema:Organization
69 Nd27acfa68570481387029ca00621b354 schema:volumeNumber 1
70 rdf:type schema:PublicationVolume
71 Nd9bc82d6df3d44ad89726692f365156a schema:name readcube_id
72 schema:value 5a6c57b5b7a22bacdc187ff928966f18a0a0dd766dd258d0c17e302519a11f0c
73 rdf:type schema:PropertyValue
74 Ne56e3800b1f649b28f88357384f8100c schema:name pubmed_id
75 schema:value 12734592
76 rdf:type schema:PropertyValue
77 Ne973377e4445435f90310b20b84fbdf5 schema:issueNumber 1
78 rdf:type schema:PublicationIssue
79 Nf5451877acd043bfad39f2069357ed5a schema:name Springer Nature - SN SciGraph project
80 rdf:type schema:Organization
81 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
82 schema:name Biological Sciences
83 rdf:type schema:DefinedTerm
84 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
85 schema:name Biochemistry and Cell Biology
86 rdf:type schema:DefinedTerm
87 sg:journal.1023701 schema:issn 1480-9222
88 schema:name Biological Procedures Online
89 rdf:type schema:Periodical
90 sg:person.01035705111.71 schema:affiliation N4fdc9dfbbdf14105a17303d46a2ca50f
91 schema:familyName Becerra
92 schema:givenName M.
93 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035705111.71
94 rdf:type schema:Person
95 sg:person.011245364025.32 schema:affiliation Na81a91308dff42d589e69f13064a7528
96 schema:familyName González Siso
97 schema:givenName M. I.
98 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32
99 rdf:type schema:Person
100 sg:person.0601204117.18 schema:affiliation N0b1be8b073f64c5497b7f75c53a675bc
101 schema:familyName Cerdán
102 schema:givenName E.
103 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601204117.18
104 rdf:type schema:Person
105 sg:pub.10.1007/bf02439313 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001623623
106 https://doi.org/10.1007/bf02439313
107 rdf:type schema:CreativeWork
108 sg:pub.10.1023/a:1008885827560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044428070
109 https://doi.org/10.1023/a:1008885827560
110 rdf:type schema:CreativeWork
111 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
112 https://doi.org/10.1038/227680a0
113 rdf:type schema:CreativeWork
114 https://app.dimensions.ai/details/publication/pub.1079679298 schema:CreativeWork
115 https://app.dimensions.ai/details/publication/pub.1080676369 schema:CreativeWork
116 https://app.dimensions.ai/details/publication/pub.1081619007 schema:CreativeWork
117 https://doi.org/10.1002/elps.1150060905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045786598
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1016/0032-9592(94)80019-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005877667
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1016/0032-9592(94)80053-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040373435
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1016/0032-9592(94)80058-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025269696
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1016/0076-6879(83)01013-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019090072
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1016/0141-0229(94)00115-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048303666
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1016/0141-0229(94)90171-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033642073
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1016/0141-0229(95)00180-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011398016
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1016/0960-8524(96)00036-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011169947
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1016/s0304-4165(97)00048-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011544794
138 rdf:type schema:CreativeWork
139 https://doi.org/10.1099/00207713-35-1-103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060347883
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1111/j.1365-3024.1993.tb00607.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013952243
142 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...