Ontology type: schema:ScholarlyArticle Open Access: True
1998-05
AUTHORSM. Becerra, E. Cerdán, M. I. González Siso
ABSTRACTSeveral micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme. More... »
PAGES48-58
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DOIhttp://dx.doi.org/10.1251/bpo4
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/12734592
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