Dealing with different methods for Kluyveromyces lactis β-galactosidase purification View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1998-05

AUTHORS

M. Becerra, E. Cerdán, M. I. González Siso

ABSTRACT

Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme. More... »

PAGES

48-58

Identifiers

URI

http://scigraph.springernature.com/pub.10.1251/bpo4

DOI

http://dx.doi.org/10.1251/bpo4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018977940

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12734592


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