OSNA-Based Novel Molecular Testing for Lymph Node Metastases in Colorectal Cancer Patients: Results from a Multicenter Clinical Performance Study in ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-02-03

AUTHORS

Hirofumi Yamamoto, Mitsugu Sekimoto, Masatoshi Oya, Noriko Yamamoto, Fumio Konishi, Junichi Sasaki, Shigeki Yamada, Kiyomi Taniyama, Harumi Tominaga, Masahiko Tsujimoto, Hiroki Akamatsu, Akio Yanagisawa, Chouhei Sakakura, Yo Kato, Nariaki Matsuura

ABSTRACT

BackgroundLymph node (LN) metastasis in colorectal cancer (CRC) is a critical factor in making accurate prognoses and therapeutic decisions. This study evaluated the clinical performance of the one-step nucleic acid amplification (OSNA) assay in accurately diagnosing LN metastases in CRC patients through the specific detection of cytokeratin 19 mRNA levels in LNs.MethodsThe OSNA assay was performed on 121 LNs dissected from early-stage CRC patients (pStage 0 or I) or from patients with benign colorectal disease (study 1). Separately, 385 LNs were dissected from 85 CRC patients (any stage); the OSNA assay was performed on half of each LN, and the results were compared with histopathological examination in 2-mm intervals of the other LN half (study 2).ResultsIn study 1, all 121 histopathologically negative LNs were also negative by the OSNA assay (concordance rate for metastasis negative: 1.0, 95% confidence interval [95% CI]: 0.976–1.0). In study 2, the concordance rate between the OSNA assay and the 2-mm-interval histopathological examination was 0.971 (95% CI: 0.950–0.984), with a sensitivity of 0.952 (95% CI: 0.881–0.987) and a specificity of 0.977 (95% CI: 0.953–0.991).ConclusionsThe OSNA assay provided a judgment performance equivalent to a 2-mm-interval histopathological examination, a more detailed assay than the common pathological examination. Therefore, the OSNA assay is considered a new molecular examination method for the diagnosis of LN metastases in CRC patients in clinical settings. More... »

PAGES

1891-1898

Journal

TITLE

Annals of Surgical Oncology

ISSUE

7

VOLUME

18

Identifiers

URI

http://scigraph.springernature.com/pub.10.1245/s10434-010-1539-5

DOI

http://dx.doi.org/10.1245/s10434-010-1539-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022698267

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21290195


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenocarcinoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenocarcinoma, Mucinous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged, 80 and over", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma, Adenosquamous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colorectal Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Japan", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Keratin-19", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphatic Metastasis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Middle Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Staging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Amplification Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Prognosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Survival Rate", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamoto", 
        "givenName": "Hirofumi", 
        "id": "sg:person.011576052022.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011576052022.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sekimoto", 
        "givenName": "Mitsugu", 
        "id": "sg:person.01347523015.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347523015.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Gastroenterological Center, Department of Gastroenterological Surgery, Cancer Institute Ariake Hospital, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Gastroenterological Center, Department of Gastroenterological Surgery, Cancer Institute Ariake Hospital, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oya", 
        "givenName": "Masatoshi", 
        "id": "sg:person.0600237645.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600237645.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.486756.e", 
          "name": [
            "Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamoto", 
        "givenName": "Noriko", 
        "id": "sg:person.014277231772.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014277231772.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan", 
          "id": "http://www.grid.ac/institutes/grid.416093.9", 
          "name": [
            "Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Konishi", 
        "givenName": "Fumio", 
        "id": "sg:person.01272216415.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272216415.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan", 
          "id": "http://www.grid.ac/institutes/grid.416093.9", 
          "name": [
            "Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sasaki", 
        "givenName": "Junichi", 
        "id": "sg:person.014130430661.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014130430661.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Saitama Medical Center, Jichi Medical University, Saitama, Japan", 
          "id": "http://www.grid.ac/institutes/grid.416093.9", 
          "name": [
            "Department of Pathology, Saitama Medical Center, Jichi Medical University, Saitama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamada", 
        "givenName": "Shigeki", 
        "id": "sg:person.01213377631.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213377631.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan", 
          "id": "http://www.grid.ac/institutes/grid.440118.8", 
          "name": [
            "Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Taniyama", 
        "givenName": "Kiyomi", 
        "id": "sg:person.0624646611.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624646611.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgery, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan", 
          "id": "http://www.grid.ac/institutes/grid.440118.8", 
          "name": [
            "Department of Surgery, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tominaga", 
        "givenName": "Harumi", 
        "id": "sg:person.01250621102.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250621102.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Osaka Police Hospital, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.416980.2", 
          "name": [
            "Department of Pathology, Osaka Police Hospital, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsujimoto", 
        "givenName": "Masahiko", 
        "id": "sg:person.01024171433.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024171433.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgery, Osaka Police Hospital, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.416980.2", 
          "name": [
            "Department of Surgery, Osaka Police Hospital, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akamatsu", 
        "givenName": "Hiroki", 
        "id": "sg:person.0760545102.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760545102.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgical Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272458.e", 
          "name": [
            "Department of Surgical Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yanagisawa", 
        "givenName": "Akio", 
        "id": "sg:person.0702701626.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702701626.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272458.e", 
          "name": [
            "Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sakakura", 
        "givenName": "Chouhei", 
        "id": "sg:person.01335405010.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335405010.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.486756.e", 
          "name": [
            "Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kato", 
        "givenName": "Yo", 
        "id": "sg:person.0614323210.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614323210.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsuura", 
        "givenName": "Nariaki", 
        "id": "sg:person.01070411170.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070411170.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1479-5876-8-83", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041411388", 
          "https://doi.org/10.1186/1479-5876-8-83"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02574511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006500436", 
          "https://doi.org/10.1007/bf02574511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00428-008-0703-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030143236", 
          "https://doi.org/10.1007/s00428-008-0703-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/bjc.1958.37", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034067018", 
          "https://doi.org/10.1038/bjc.1958.37"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1245/s10434-006-9120-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000997664", 
          "https://doi.org/10.1245/s10434-006-9120-y"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-02-03", 
    "datePublishedReg": "2011-02-03", 
    "description": "BackgroundLymph node (LN) metastasis in colorectal cancer (CRC) is a critical factor in making accurate prognoses and therapeutic decisions. This study evaluated the clinical performance of the one-step nucleic acid amplification (OSNA) assay in accurately diagnosing LN metastases in CRC patients through the specific detection of cytokeratin 19 mRNA levels in LNs.MethodsThe OSNA assay was performed on 121 LNs dissected from early-stage CRC patients (pStage 0 or I) or from patients with benign colorectal disease (study 1). Separately, 385 LNs were dissected from 85 CRC patients (any stage); the OSNA assay was performed on half of each LN, and the results were compared with histopathological examination in 2-mm intervals of the other LN half (study 2).ResultsIn study 1, all 121 histopathologically negative LNs were also negative by the OSNA assay (concordance rate for metastasis negative: 1.0, 95% confidence interval [95% CI]: 0.976\u20131.0). In study 2, the concordance rate between the OSNA assay and the 2-mm-interval histopathological examination was 0.971 (95% CI: 0.950\u20130.984), with a sensitivity of 0.952 (95% CI: 0.881\u20130.987) and a specificity of 0.977 (95% CI: 0.953\u20130.991).ConclusionsThe OSNA assay provided a judgment performance equivalent to a 2-mm-interval histopathological examination, a more detailed assay than the common pathological examination. Therefore, the OSNA assay is considered a new molecular examination method for the diagnosis of LN metastases in CRC patients in clinical settings.", 
    "genre": "article", 
    "id": "sg:pub.10.1245/s10434-010-1539-5", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6059585", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105545", 
        "issn": [
          "1068-9265", 
          "1534-4681"
        ], 
        "name": "Annals of Surgical Oncology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "keywords": [
      "one-step nucleic acid amplification", 
      "CRC patients", 
      "histopathological examination", 
      "LN metastasis", 
      "node metastasis", 
      "colorectal cancer", 
      "early-stage CRC patients", 
      "BackgroundLymph node metastasis", 
      "novel molecular testing", 
      "benign colorectal diseases", 
      "lymph node metastasis", 
      "colorectal cancer patients", 
      "clinical performance studies", 
      "negative LNs", 
      "pathological examination", 
      "colorectal disease", 
      "OSNA assay", 
      "cancer patients", 
      "therapeutic decisions", 
      "patients", 
      "molecular testing", 
      "nucleic acid amplification", 
      "metastasis", 
      "accurate prognosis", 
      "concordance rate", 
      "clinical setting", 
      "ResultsIn study 1", 
      "clinical performance", 
      "mRNA levels", 
      "examination methods", 
      "acid amplification", 
      "examination", 
      "prognosis", 
      "cancer", 
      "disease", 
      "diagnosis", 
      "LNs", 
      "half", 
      "study", 
      "Study 2", 
      "assays", 
      "Study 1", 
      "Ln", 
      "specificity", 
      "setting", 
      "specific detection", 
      "interval", 
      "factors", 
      "levels", 
      "testing", 
      "critical factor", 
      "sensitivity", 
      "rate", 
      "results", 
      "amplification", 
      "detection", 
      "Japan", 
      "decisions", 
      "judgment performance", 
      "method", 
      "performance", 
      "performance study"
    ], 
    "name": "OSNA-Based Novel Molecular Testing for Lymph Node Metastases in Colorectal Cancer Patients: Results from a Multicenter Clinical Performance Study in Japan", 
    "pagination": "1891-1898", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1022698267"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1245/s10434-010-1539-5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21290195"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1245/s10434-010-1539-5", 
      "https://app.dimensions.ai/details/publication/pub.1022698267"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_549.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1245/s10434-010-1539-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1245/s10434-010-1539-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1245/s10434-010-1539-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1245/s10434-010-1539-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1245/s10434-010-1539-5'


 

This table displays all metadata directly associated to this object as RDF triples.

348 TRIPLES      22 PREDICATES      112 URIs      99 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1245/s10434-010-1539-5 schema:about N047138043f354cc1853d73abd69cd194
2 N098544e44241482db75b31fa70db6db3
3 N0d9d0ee7d49a4e57946bf8c6e17226cc
4 N26b38320904f42cfa9ee9008ab3c3b7b
5 N3166c3742aae4860aaa2ac2ecb558cab
6 N39744fd11e354ec1a3d9f0b8ffc90b4a
7 N3d6fc6890e5a4840a480cc17a248d1d9
8 N44b0de0ef0e44aa78232a77700aaf14a
9 N45a32fdbaf684cef9de72e66ff310643
10 N4d0021b520804583b568b43b964a55ed
11 N85a2341b4fa64ce682d01f79573a462d
12 N989c0913df1249698597f16f324c18cd
13 N9b35ce75907b4283ba4e5ff0665ec4c3
14 Nc4752f5c8d8d44b4a6611e3ce61cfe86
15 Nd215a89a466b48f588b580a09d4bef9d
16 Nd4376a19a7fe40eda6ae57ff67135878
17 Ne151b9ac5c834076aa3aea3988ec5ceb
18 Neddf47df1380468184e16def70196d4e
19 Nf4d7a9267e444fa6bd780d9b23e83d73
20 anzsrc-for:11
21 anzsrc-for:1112
22 schema:author N5c738eaa483e4044a693e337097f5e59
23 schema:citation sg:pub.10.1007/bf02574511
24 sg:pub.10.1007/s00428-008-0703-9
25 sg:pub.10.1038/bjc.1958.37
26 sg:pub.10.1186/1479-5876-8-83
27 sg:pub.10.1245/s10434-006-9120-y
28 schema:datePublished 2011-02-03
29 schema:datePublishedReg 2011-02-03
30 schema:description BackgroundLymph node (LN) metastasis in colorectal cancer (CRC) is a critical factor in making accurate prognoses and therapeutic decisions. This study evaluated the clinical performance of the one-step nucleic acid amplification (OSNA) assay in accurately diagnosing LN metastases in CRC patients through the specific detection of cytokeratin 19 mRNA levels in LNs.MethodsThe OSNA assay was performed on 121 LNs dissected from early-stage CRC patients (pStage 0 or I) or from patients with benign colorectal disease (study 1). Separately, 385 LNs were dissected from 85 CRC patients (any stage); the OSNA assay was performed on half of each LN, and the results were compared with histopathological examination in 2-mm intervals of the other LN half (study 2).ResultsIn study 1, all 121 histopathologically negative LNs were also negative by the OSNA assay (concordance rate for metastasis negative: 1.0, 95% confidence interval [95% CI]: 0.976–1.0). In study 2, the concordance rate between the OSNA assay and the 2-mm-interval histopathological examination was 0.971 (95% CI: 0.950–0.984), with a sensitivity of 0.952 (95% CI: 0.881–0.987) and a specificity of 0.977 (95% CI: 0.953–0.991).ConclusionsThe OSNA assay provided a judgment performance equivalent to a 2-mm-interval histopathological examination, a more detailed assay than the common pathological examination. Therefore, the OSNA assay is considered a new molecular examination method for the diagnosis of LN metastases in CRC patients in clinical settings.
31 schema:genre article
32 schema:inLanguage en
33 schema:isAccessibleForFree false
34 schema:isPartOf N724b841738df41a3adf1f705b2e8f46f
35 N7b86443e42414ee2b23baedebd1df1dc
36 sg:journal.1105545
37 schema:keywords BackgroundLymph node metastasis
38 CRC patients
39 Japan
40 LN metastasis
41 LNs
42 Ln
43 OSNA assay
44 ResultsIn study 1
45 Study 1
46 Study 2
47 accurate prognosis
48 acid amplification
49 amplification
50 assays
51 benign colorectal diseases
52 cancer
53 cancer patients
54 clinical performance
55 clinical performance studies
56 clinical setting
57 colorectal cancer
58 colorectal cancer patients
59 colorectal disease
60 concordance rate
61 critical factor
62 decisions
63 detection
64 diagnosis
65 disease
66 early-stage CRC patients
67 examination
68 examination methods
69 factors
70 half
71 histopathological examination
72 interval
73 judgment performance
74 levels
75 lymph node metastasis
76 mRNA levels
77 metastasis
78 method
79 molecular testing
80 negative LNs
81 node metastasis
82 novel molecular testing
83 nucleic acid amplification
84 one-step nucleic acid amplification
85 pathological examination
86 patients
87 performance
88 performance study
89 prognosis
90 rate
91 results
92 sensitivity
93 setting
94 specific detection
95 specificity
96 study
97 testing
98 therapeutic decisions
99 schema:name OSNA-Based Novel Molecular Testing for Lymph Node Metastases in Colorectal Cancer Patients: Results from a Multicenter Clinical Performance Study in Japan
100 schema:pagination 1891-1898
101 schema:productId N117fd5d02af3428ca616f4eab3dd7672
102 N5327e4bd21bc4ac2927837c88a163a4a
103 Nd32f20591f0d451ba6ee92f0b006095f
104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022698267
105 https://doi.org/10.1245/s10434-010-1539-5
106 schema:sdDatePublished 2022-05-20T07:27
107 schema:sdLicense https://scigraph.springernature.com/explorer/license/
108 schema:sdPublisher N15e6bd9241fb45d8ac7050a079a8c6ba
109 schema:url https://doi.org/10.1245/s10434-010-1539-5
110 sgo:license sg:explorer/license/
111 sgo:sdDataset articles
112 rdf:type schema:ScholarlyArticle
113 N047138043f354cc1853d73abd69cd194 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Adenocarcinoma, Mucinous
115 rdf:type schema:DefinedTerm
116 N0611c53c3b0445ca812b2e546a15bcbb rdf:first sg:person.014130430661.38
117 rdf:rest N53ee0cbd25934f28ab2f4d65adde8d9b
118 N098544e44241482db75b31fa70db6db3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Middle Aged
120 rdf:type schema:DefinedTerm
121 N0d9d0ee7d49a4e57946bf8c6e17226cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Biomarkers, Tumor
123 rdf:type schema:DefinedTerm
124 N117fd5d02af3428ca616f4eab3dd7672 schema:name dimensions_id
125 schema:value pub.1022698267
126 rdf:type schema:PropertyValue
127 N15e6bd9241fb45d8ac7050a079a8c6ba schema:name Springer Nature - SN SciGraph project
128 rdf:type schema:Organization
129 N23bf54e08f8d4f2c935b4e43cc984005 rdf:first sg:person.0600237645.04
130 rdf:rest N93f4ebddff8a42fba48de63f90999b6b
131 N2625ab1d964941608a849e517e7bcac8 rdf:first sg:person.01024171433.62
132 rdf:rest Nbf7241ed156142e499602619a5bae561
133 N26b38320904f42cfa9ee9008ab3c3b7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Prognosis
135 rdf:type schema:DefinedTerm
136 N3166c3742aae4860aaa2ac2ecb558cab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Aged
138 rdf:type schema:DefinedTerm
139 N343014d4cd3341de8825936fb48aaaca rdf:first sg:person.01347523015.93
140 rdf:rest N23bf54e08f8d4f2c935b4e43cc984005
141 N39744fd11e354ec1a3d9f0b8ffc90b4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Female
143 rdf:type schema:DefinedTerm
144 N3d6fc6890e5a4840a480cc17a248d1d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Neoplasm Staging
146 rdf:type schema:DefinedTerm
147 N44b0de0ef0e44aa78232a77700aaf14a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Keratin-19
149 rdf:type schema:DefinedTerm
150 N45a32fdbaf684cef9de72e66ff310643 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Carcinoma, Adenosquamous
152 rdf:type schema:DefinedTerm
153 N4871730257d74454bcd7c89babb718cc rdf:first sg:person.01250621102.61
154 rdf:rest N2625ab1d964941608a849e517e7bcac8
155 N4d0021b520804583b568b43b964a55ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Survival Rate
157 rdf:type schema:DefinedTerm
158 N5327e4bd21bc4ac2927837c88a163a4a schema:name doi
159 schema:value 10.1245/s10434-010-1539-5
160 rdf:type schema:PropertyValue
161 N537f6b11a61841fe85b191d37b2e9239 rdf:first sg:person.0624646611.06
162 rdf:rest N4871730257d74454bcd7c89babb718cc
163 N53ee0cbd25934f28ab2f4d65adde8d9b rdf:first sg:person.01213377631.92
164 rdf:rest N537f6b11a61841fe85b191d37b2e9239
165 N5c738eaa483e4044a693e337097f5e59 rdf:first sg:person.011576052022.32
166 rdf:rest N343014d4cd3341de8825936fb48aaaca
167 N6dcf905b4cdf460d945e101c70eaffcb rdf:first sg:person.01335405010.35
168 rdf:rest Ne0b246680c6349339b3f17eecc04599d
169 N724b841738df41a3adf1f705b2e8f46f schema:issueNumber 7
170 rdf:type schema:PublicationIssue
171 N7b86443e42414ee2b23baedebd1df1dc schema:volumeNumber 18
172 rdf:type schema:PublicationVolume
173 N82e56ff1e72243fbb036df12cc9138b4 rdf:first sg:person.01070411170.48
174 rdf:rest rdf:nil
175 N85a2341b4fa64ce682d01f79573a462d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Humans
177 rdf:type schema:DefinedTerm
178 N93f4ebddff8a42fba48de63f90999b6b rdf:first sg:person.014277231772.83
179 rdf:rest Nd6626f50d4ac41e0bd7533a0feb09d5e
180 N989c0913df1249698597f16f324c18cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Aged, 80 and over
182 rdf:type schema:DefinedTerm
183 N9b35ce75907b4283ba4e5ff0665ec4c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Nucleic Acid Amplification Techniques
185 rdf:type schema:DefinedTerm
186 Nbf7241ed156142e499602619a5bae561 rdf:first sg:person.0760545102.14
187 rdf:rest Nf4224e968faf4102baf7351cbd78608b
188 Nc4752f5c8d8d44b4a6611e3ce61cfe86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Adult
190 rdf:type schema:DefinedTerm
191 Nd215a89a466b48f588b580a09d4bef9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Lymphatic Metastasis
193 rdf:type schema:DefinedTerm
194 Nd32f20591f0d451ba6ee92f0b006095f schema:name pubmed_id
195 schema:value 21290195
196 rdf:type schema:PropertyValue
197 Nd4376a19a7fe40eda6ae57ff67135878 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Colorectal Neoplasms
199 rdf:type schema:DefinedTerm
200 Nd6626f50d4ac41e0bd7533a0feb09d5e rdf:first sg:person.01272216415.22
201 rdf:rest N0611c53c3b0445ca812b2e546a15bcbb
202 Ne0b246680c6349339b3f17eecc04599d rdf:first sg:person.0614323210.10
203 rdf:rest N82e56ff1e72243fbb036df12cc9138b4
204 Ne151b9ac5c834076aa3aea3988ec5ceb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
205 schema:name Adenocarcinoma
206 rdf:type schema:DefinedTerm
207 Neddf47df1380468184e16def70196d4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
208 schema:name Japan
209 rdf:type schema:DefinedTerm
210 Nf4224e968faf4102baf7351cbd78608b rdf:first sg:person.0702701626.77
211 rdf:rest N6dcf905b4cdf460d945e101c70eaffcb
212 Nf4d7a9267e444fa6bd780d9b23e83d73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Male
214 rdf:type schema:DefinedTerm
215 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
216 schema:name Medical and Health Sciences
217 rdf:type schema:DefinedTerm
218 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
219 schema:name Oncology and Carcinogenesis
220 rdf:type schema:DefinedTerm
221 sg:grant.6059585 http://pending.schema.org/fundedItem sg:pub.10.1245/s10434-010-1539-5
222 rdf:type schema:MonetaryGrant
223 sg:journal.1105545 schema:issn 1068-9265
224 1534-4681
225 schema:name Annals of Surgical Oncology
226 schema:publisher Springer Nature
227 rdf:type schema:Periodical
228 sg:person.01024171433.62 schema:affiliation grid-institutes:grid.416980.2
229 schema:familyName Tsujimoto
230 schema:givenName Masahiko
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024171433.62
232 rdf:type schema:Person
233 sg:person.01070411170.48 schema:affiliation grid-institutes:grid.136593.b
234 schema:familyName Matsuura
235 schema:givenName Nariaki
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070411170.48
237 rdf:type schema:Person
238 sg:person.011576052022.32 schema:affiliation grid-institutes:grid.136593.b
239 schema:familyName Yamamoto
240 schema:givenName Hirofumi
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011576052022.32
242 rdf:type schema:Person
243 sg:person.01213377631.92 schema:affiliation grid-institutes:grid.416093.9
244 schema:familyName Yamada
245 schema:givenName Shigeki
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213377631.92
247 rdf:type schema:Person
248 sg:person.01250621102.61 schema:affiliation grid-institutes:grid.440118.8
249 schema:familyName Tominaga
250 schema:givenName Harumi
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250621102.61
252 rdf:type schema:Person
253 sg:person.01272216415.22 schema:affiliation grid-institutes:grid.416093.9
254 schema:familyName Konishi
255 schema:givenName Fumio
256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272216415.22
257 rdf:type schema:Person
258 sg:person.01335405010.35 schema:affiliation grid-institutes:grid.272458.e
259 schema:familyName Sakakura
260 schema:givenName Chouhei
261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335405010.35
262 rdf:type schema:Person
263 sg:person.01347523015.93 schema:affiliation grid-institutes:grid.136593.b
264 schema:familyName Sekimoto
265 schema:givenName Mitsugu
266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347523015.93
267 rdf:type schema:Person
268 sg:person.014130430661.38 schema:affiliation grid-institutes:grid.416093.9
269 schema:familyName Sasaki
270 schema:givenName Junichi
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014130430661.38
272 rdf:type schema:Person
273 sg:person.014277231772.83 schema:affiliation grid-institutes:grid.486756.e
274 schema:familyName Yamamoto
275 schema:givenName Noriko
276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014277231772.83
277 rdf:type schema:Person
278 sg:person.0600237645.04 schema:affiliation grid-institutes:None
279 schema:familyName Oya
280 schema:givenName Masatoshi
281 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600237645.04
282 rdf:type schema:Person
283 sg:person.0614323210.10 schema:affiliation grid-institutes:grid.486756.e
284 schema:familyName Kato
285 schema:givenName Yo
286 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614323210.10
287 rdf:type schema:Person
288 sg:person.0624646611.06 schema:affiliation grid-institutes:grid.440118.8
289 schema:familyName Taniyama
290 schema:givenName Kiyomi
291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624646611.06
292 rdf:type schema:Person
293 sg:person.0702701626.77 schema:affiliation grid-institutes:grid.272458.e
294 schema:familyName Yanagisawa
295 schema:givenName Akio
296 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702701626.77
297 rdf:type schema:Person
298 sg:person.0760545102.14 schema:affiliation grid-institutes:grid.416980.2
299 schema:familyName Akamatsu
300 schema:givenName Hiroki
301 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760545102.14
302 rdf:type schema:Person
303 sg:pub.10.1007/bf02574511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006500436
304 https://doi.org/10.1007/bf02574511
305 rdf:type schema:CreativeWork
306 sg:pub.10.1007/s00428-008-0703-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030143236
307 https://doi.org/10.1007/s00428-008-0703-9
308 rdf:type schema:CreativeWork
309 sg:pub.10.1038/bjc.1958.37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034067018
310 https://doi.org/10.1038/bjc.1958.37
311 rdf:type schema:CreativeWork
312 sg:pub.10.1186/1479-5876-8-83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041411388
313 https://doi.org/10.1186/1479-5876-8-83
314 rdf:type schema:CreativeWork
315 sg:pub.10.1245/s10434-006-9120-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1000997664
316 https://doi.org/10.1245/s10434-006-9120-y
317 rdf:type schema:CreativeWork
318 grid-institutes:None schema:alternateName Gastroenterological Center, Department of Gastroenterological Surgery, Cancer Institute Ariake Hospital, Tokyo, Japan
319 schema:name Gastroenterological Center, Department of Gastroenterological Surgery, Cancer Institute Ariake Hospital, Tokyo, Japan
320 rdf:type schema:Organization
321 grid-institutes:grid.136593.b schema:alternateName Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Osaka, Japan
322 Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan
323 schema:name Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Osaka, Japan
324 Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan
325 rdf:type schema:Organization
326 grid-institutes:grid.272458.e schema:alternateName Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan
327 Department of Surgical Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan
328 schema:name Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan
329 Department of Surgical Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan
330 rdf:type schema:Organization
331 grid-institutes:grid.416093.9 schema:alternateName Department of Pathology, Saitama Medical Center, Jichi Medical University, Saitama, Japan
332 Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan
333 schema:name Department of Pathology, Saitama Medical Center, Jichi Medical University, Saitama, Japan
334 Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan
335 rdf:type schema:Organization
336 grid-institutes:grid.416980.2 schema:alternateName Department of Pathology, Osaka Police Hospital, Osaka, Japan
337 Department of Surgery, Osaka Police Hospital, Osaka, Japan
338 schema:name Department of Pathology, Osaka Police Hospital, Osaka, Japan
339 Department of Surgery, Osaka Police Hospital, Osaka, Japan
340 rdf:type schema:Organization
341 grid-institutes:grid.440118.8 schema:alternateName Department of Surgery, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan
342 Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan
343 schema:name Department of Surgery, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan
344 Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Hiroshima, Japan
345 rdf:type schema:Organization
346 grid-institutes:grid.486756.e schema:alternateName Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan
347 schema:name Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan
348 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...