Annexin II Overexpression Predicts Rapid Recurrence after Surgery in Pancreatic Cancer Patients Undergoing Gemcitabine-Adjuvant Chemotherapy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-08-20

AUTHORS

Shigetsugu Takano, Akira Togawa, Hideyuki Yoshitomi, Takashi Shida, Fumio Kimura, Hiroaki Shimizu, Hiroyuki Yoshidome, Masayuki Ohtsuka, Atsushi Kato, Takeshi Tomonaga, Fumio Nomura, Masaru Miyazaki

ABSTRACT

BackgroundGemcitabine has been shown to exhibit significant clinical activity against pancreatic cancer and has become a first-line chemotherapeutic for this disease in recent years. However, there are still many patients who do not respond to this treatment and it is expected to improve the clinical outcome if we can develop a method to predict the efficacy of gemcitabine before treatment. The purpose of this study was to determine novel factors that make pancreatic cancer resistant to gemcitabine.Materials and methodsUsing the high-resolution proteomic approach, agarose two-dimensional gel electrophoresis, we compared protein profiling of a gemcitabine-resistant pancreatic cancer cell line with its wild-type.ResultsWe identified Annexin II as an up-regulated protein in the gemcitabine-resistant pancreatic cancer cell line. Immunohistochemistry demonstrated that Annexin II was mainly expressed at the cell surface of pancreatic cancer cells. Interestingly, Annexin II overexpression in cancer cells was significantly associated with rapid recurrence after gemcitabine adjuvant chemotherapy in postoperative patients (P = .0078), and its staining was also an independent prognostic indicator of recurrence in pancreatic cancer patients who underwent adjuvant gemcitabine treatment after curative surgery on multivariate analysis (P = .0047). In addition, inhibition of Annexin II expression by siRNA in pancreatic cancer cell lines increased the cytotoxic efficacy of gemcitabine. These results indicate that Annexin II overexpression may induce gemcitabine resistance in pancreatic cancer resulting in rapid recurrence.ConclusionsAnalysis of Annexin II expression in cancer tissues may predict the clinical outcome of gemcitabine treatment, leading to the development of a new method for tailor-made treatment for this disease. More... »

PAGES

3157-3168

Identifiers

URI

http://scigraph.springernature.com/pub.10.1245/s10434-008-0061-5

DOI

http://dx.doi.org/10.1245/s10434-008-0061-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011437644

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18712570


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenocarcinoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Annexin A2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antimetabolites, Antineoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Western", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma, Adenosquamous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma, Pancreatic Ductal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chemotherapy, Adjuvant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Deoxycytidine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance, Neoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Neoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoenzyme Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Middle Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Invasiveness", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Recurrence, Local", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pancreatic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Prognosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Small Interfering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Survival Rate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
            "Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takano", 
        "givenName": "Shigetsugu", 
        "id": "sg:person.01266546053.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266546053.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Togawa", 
        "givenName": "Akira", 
        "id": "sg:person.0660326574.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660326574.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoshitomi", 
        "givenName": "Hideyuki", 
        "id": "sg:person.01245062664.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245062664.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shida", 
        "givenName": "Takashi", 
        "id": "sg:person.01054077577.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054077577.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kimura", 
        "givenName": "Fumio", 
        "id": "sg:person.01127754745.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127754745.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shimizu", 
        "givenName": "Hiroaki", 
        "id": "sg:person.01103413111.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103413111.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoshidome", 
        "givenName": "Hiroyuki", 
        "id": "sg:person.016423226454.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016423226454.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ohtsuka", 
        "givenName": "Masayuki", 
        "id": "sg:person.0653527253.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653527253.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kato", 
        "givenName": "Atsushi", 
        "id": "sg:person.01354163205.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354163205.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tomonaga", 
        "givenName": "Takeshi", 
        "id": "sg:person.01371003243.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371003243.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nomura", 
        "givenName": "Fumio", 
        "id": "sg:person.0723474205.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723474205.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136304.3", 
          "name": [
            "The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyazaki", 
        "givenName": "Masaru", 
        "id": "sg:person.0773025611.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773025611.35"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nrc949", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009614296", 
          "https://doi.org/10.1038/nrc949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1005953000523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009957264", 
          "https://doi.org/10.1023/a:1005953000523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/bjc.1998.750", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002720064", 
          "https://doi.org/10.1038/bjc.1998.750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1210951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019746269", 
          "https://doi.org/10.1038/sj.onc.1210951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11010-007-9649-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022402190", 
          "https://doi.org/10.1007/s11010-007-9649-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/bjc.1992.103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044981451", 
          "https://doi.org/10.1038/bjc.1992.103"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-08-20", 
    "datePublishedReg": "2008-08-20", 
    "description": "BackgroundGemcitabine has been shown to exhibit significant clinical activity against pancreatic cancer and has become a first-line chemotherapeutic for this disease in recent years. However, there are still many patients who do not respond to this treatment and it is expected to improve the clinical outcome if we can develop a method to predict the efficacy of gemcitabine before treatment. The purpose of this study was to determine novel factors that make pancreatic cancer resistant to gemcitabine.Materials and methodsUsing the high-resolution proteomic approach, agarose two-dimensional gel electrophoresis, we compared protein profiling of a gemcitabine-resistant pancreatic cancer cell line with its wild-type.ResultsWe identified Annexin II as an up-regulated protein in the gemcitabine-resistant pancreatic cancer cell line. Immunohistochemistry demonstrated that Annexin II was mainly expressed at the cell surface of pancreatic cancer cells. Interestingly, Annexin II overexpression in cancer cells was significantly associated with rapid recurrence after gemcitabine adjuvant chemotherapy in postoperative patients (P\u00a0=\u00a0.0078), and its staining was also an independent prognostic indicator of recurrence in pancreatic cancer patients who underwent adjuvant gemcitabine treatment after curative surgery on multivariate analysis (P\u00a0=\u00a0.0047). In addition, inhibition of Annexin II expression by siRNA in pancreatic cancer cell lines increased the cytotoxic efficacy of gemcitabine. These results indicate that Annexin II overexpression may induce gemcitabine resistance in pancreatic cancer resulting in rapid recurrence.ConclusionsAnalysis of Annexin II expression in cancer tissues may predict the clinical outcome of gemcitabine treatment, leading to the development of a new method for tailor-made treatment for this disease.", 
    "genre": "article", 
    "id": "sg:pub.10.1245/s10434-008-0061-5", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1105545", 
        "issn": [
          "1068-9265", 
          "1534-4681"
        ], 
        "name": "Annals of Surgical Oncology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "11", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "keywords": [
      "pancreatic cancer cell lines", 
      "gemcitabine-resistant pancreatic cancer cell lines", 
      "gemcitabine adjuvant chemotherapy", 
      "pancreatic cancer patients", 
      "cancer cell lines", 
      "rapid recurrence", 
      "pancreatic cancer", 
      "clinical outcomes", 
      "cancer patients", 
      "gemcitabine treatment", 
      "cell lines", 
      "significant clinical activity", 
      "adjuvant gemcitabine treatment", 
      "efficacy of gemcitabine", 
      "cancer cells", 
      "independent prognostic indicator", 
      "high-resolution proteomics approaches", 
      "Annexin II expression", 
      "tailor-made treatment", 
      "pancreatic cancer cells", 
      "Annexin II", 
      "adjuvant chemotherapy", 
      "curative surgery", 
      "postoperative patients", 
      "prognostic indicator", 
      "clinical activity", 
      "gemcitabine resistance", 
      "cancer tissues", 
      "patients", 
      "multivariate analysis", 
      "recurrence", 
      "gemcitabine", 
      "cancer", 
      "cytotoxic efficacy", 
      "chemotherapy", 
      "treatment", 
      "surgery", 
      "disease", 
      "efficacy", 
      "novel factor", 
      "overexpression", 
      "outcomes", 
      "cell surface", 
      "two-dimensional gel electrophoresis", 
      "cells", 
      "BackgroundGemcitabine", 
      "immunohistochemistry", 
      "expression", 
      "proteomic approach", 
      "ResultsWe", 
      "staining", 
      "protein profiling", 
      "siRNA", 
      "gel electrophoresis", 
      "inhibition", 
      "tissue", 
      "years", 
      "ConclusionsAnalysis", 
      "lines", 
      "factors", 
      "activity", 
      "protein", 
      "study", 
      "profiling", 
      "resistance", 
      "recent years", 
      "indicators", 
      "purpose", 
      "electrophoresis", 
      "addition", 
      "development", 
      "method", 
      "analysis", 
      "results", 
      "materials", 
      "approach", 
      "new method", 
      "surface"
    ], 
    "name": "Annexin II Overexpression Predicts Rapid Recurrence after Surgery in Pancreatic Cancer Patients Undergoing Gemcitabine-Adjuvant Chemotherapy", 
    "pagination": "3157-3168", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011437644"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1245/s10434-008-0061-5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18712570"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1245/s10434-008-0061-5", 
      "https://app.dimensions.ai/details/publication/pub.1011437644"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T15:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_475.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1245/s10434-008-0061-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1245/s10434-008-0061-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1245/s10434-008-0061-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1245/s10434-008-0061-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1245/s10434-008-0061-5'


 

This table displays all metadata directly associated to this object as RDF triples.

346 TRIPLES      21 PREDICATES      135 URIs      121 LITERALS      33 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1245/s10434-008-0061-5 schema:about N01a7544ec0ab4b6ba45cac166cccebfe
2 N02547699d1514968871f4e1eb9af266c
3 N0887b88a86054705897f9cd86854d22a
4 N10c6a23ce5bb48a593b0376b0c5b0634
5 N12182f3ccec6402bad6eb44aee50a812
6 N14177fc2d22c4abba549f9e9a65a8ded
7 N27d15ca3bc76464b85af77b9c7290449
8 N2914842d30a44824bab0e7259fdf9d3e
9 N314aa43f27224ed28a3932e72c944542
10 N42cd79931966424ebf4271e1d46ad684
11 N65cc4850a8df4731b42200aada9cd051
12 N66ca5d8253874f0b80a19490b95a514d
13 N72a1d8a4de074f039d6874479566e0f2
14 N83844272d28741fe9ba0a6831f5380c1
15 N8a8897b13bf6450683d61db53ccceeea
16 N8fde297cb6a24ef290d3679e26479ef5
17 Na554318f88cf47c38b693a7030bf1caa
18 Nb1cb91a0016e44c091d72324cbdaa901
19 Nbbdc2f47d0854d5abb28dae854b95356
20 Nbdf0a8178a594a48a34746117abf09e3
21 Ncb9737cc37b1447d92335390158bf5e7
22 Ncc20f618cb144fbe8c71d9c09916cedc
23 Ned33daf1c1be4fe8a8d0a321ca799f60
24 Nf61d69aab12b461fbb17df76e5d33d65
25 Nfd57afe98dcc4a8e99ad203e877f943f
26 Nff2251c9f3824f7aa29bbd85c97ffe50
27 anzsrc-for:11
28 anzsrc-for:1112
29 schema:author N8f6eb3737b78411096248eeb4c38c49c
30 schema:citation sg:pub.10.1007/s11010-007-9649-5
31 sg:pub.10.1023/a:1005953000523
32 sg:pub.10.1038/bjc.1992.103
33 sg:pub.10.1038/bjc.1998.750
34 sg:pub.10.1038/nrc949
35 sg:pub.10.1038/sj.onc.1210951
36 schema:datePublished 2008-08-20
37 schema:datePublishedReg 2008-08-20
38 schema:description BackgroundGemcitabine has been shown to exhibit significant clinical activity against pancreatic cancer and has become a first-line chemotherapeutic for this disease in recent years. However, there are still many patients who do not respond to this treatment and it is expected to improve the clinical outcome if we can develop a method to predict the efficacy of gemcitabine before treatment. The purpose of this study was to determine novel factors that make pancreatic cancer resistant to gemcitabine.Materials and methodsUsing the high-resolution proteomic approach, agarose two-dimensional gel electrophoresis, we compared protein profiling of a gemcitabine-resistant pancreatic cancer cell line with its wild-type.ResultsWe identified Annexin II as an up-regulated protein in the gemcitabine-resistant pancreatic cancer cell line. Immunohistochemistry demonstrated that Annexin II was mainly expressed at the cell surface of pancreatic cancer cells. Interestingly, Annexin II overexpression in cancer cells was significantly associated with rapid recurrence after gemcitabine adjuvant chemotherapy in postoperative patients (P = .0078), and its staining was also an independent prognostic indicator of recurrence in pancreatic cancer patients who underwent adjuvant gemcitabine treatment after curative surgery on multivariate analysis (P = .0047). In addition, inhibition of Annexin II expression by siRNA in pancreatic cancer cell lines increased the cytotoxic efficacy of gemcitabine. These results indicate that Annexin II overexpression may induce gemcitabine resistance in pancreatic cancer resulting in rapid recurrence.ConclusionsAnalysis of Annexin II expression in cancer tissues may predict the clinical outcome of gemcitabine treatment, leading to the development of a new method for tailor-made treatment for this disease.
39 schema:genre article
40 schema:isAccessibleForFree false
41 schema:isPartOf N02abb3f9f9f34be6b650720908a8c71c
42 Ne9559ac712b148f78a60939163536d9b
43 sg:journal.1105545
44 schema:keywords Annexin II
45 Annexin II expression
46 BackgroundGemcitabine
47 ConclusionsAnalysis
48 ResultsWe
49 activity
50 addition
51 adjuvant chemotherapy
52 adjuvant gemcitabine treatment
53 analysis
54 approach
55 cancer
56 cancer cell lines
57 cancer cells
58 cancer patients
59 cancer tissues
60 cell lines
61 cell surface
62 cells
63 chemotherapy
64 clinical activity
65 clinical outcomes
66 curative surgery
67 cytotoxic efficacy
68 development
69 disease
70 efficacy
71 efficacy of gemcitabine
72 electrophoresis
73 expression
74 factors
75 gel electrophoresis
76 gemcitabine
77 gemcitabine adjuvant chemotherapy
78 gemcitabine resistance
79 gemcitabine treatment
80 gemcitabine-resistant pancreatic cancer cell lines
81 high-resolution proteomics approaches
82 immunohistochemistry
83 independent prognostic indicator
84 indicators
85 inhibition
86 lines
87 materials
88 method
89 multivariate analysis
90 new method
91 novel factor
92 outcomes
93 overexpression
94 pancreatic cancer
95 pancreatic cancer cell lines
96 pancreatic cancer cells
97 pancreatic cancer patients
98 patients
99 postoperative patients
100 profiling
101 prognostic indicator
102 protein
103 protein profiling
104 proteomic approach
105 purpose
106 rapid recurrence
107 recent years
108 recurrence
109 resistance
110 results
111 siRNA
112 significant clinical activity
113 staining
114 study
115 surface
116 surgery
117 tailor-made treatment
118 tissue
119 treatment
120 two-dimensional gel electrophoresis
121 years
122 schema:name Annexin II Overexpression Predicts Rapid Recurrence after Surgery in Pancreatic Cancer Patients Undergoing Gemcitabine-Adjuvant Chemotherapy
123 schema:pagination 3157-3168
124 schema:productId N1defa095626343f6bfc71340068b2acb
125 N2ae395bcb18b48d0a7dbe68a4d307f03
126 N77703b91a21c4dafa24b1ed87a19f7bf
127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011437644
128 https://doi.org/10.1245/s10434-008-0061-5
129 schema:sdDatePublished 2022-09-02T15:52
130 schema:sdLicense https://scigraph.springernature.com/explorer/license/
131 schema:sdPublisher N9aefd8db35414c3ea724650c9a7ea21d
132 schema:url https://doi.org/10.1245/s10434-008-0061-5
133 sgo:license sg:explorer/license/
134 sgo:sdDataset articles
135 rdf:type schema:ScholarlyArticle
136 N004cf3c5b16341dab43278c040df2f5c rdf:first sg:person.01103413111.45
137 rdf:rest N72afb4feab4b46c69980e4b3a9746b6c
138 N01a7544ec0ab4b6ba45cac166cccebfe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Antimetabolites, Antineoplastic
140 rdf:type schema:DefinedTerm
141 N02547699d1514968871f4e1eb9af266c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name RNA, Small Interfering
143 rdf:type schema:DefinedTerm
144 N02abb3f9f9f34be6b650720908a8c71c schema:issueNumber 11
145 rdf:type schema:PublicationIssue
146 N0887b88a86054705897f9cd86854d22a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Survival Rate
148 rdf:type schema:DefinedTerm
149 N10c6a23ce5bb48a593b0376b0c5b0634 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Cell Survival
151 rdf:type schema:DefinedTerm
152 N12182f3ccec6402bad6eb44aee50a812 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Reverse Transcriptase Polymerase Chain Reaction
154 rdf:type schema:DefinedTerm
155 N14177fc2d22c4abba549f9e9a65a8ded schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Pancreatic Neoplasms
157 rdf:type schema:DefinedTerm
158 N1defa095626343f6bfc71340068b2acb schema:name pubmed_id
159 schema:value 18712570
160 rdf:type schema:PropertyValue
161 N27d15ca3bc76464b85af77b9c7290449 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Humans
163 rdf:type schema:DefinedTerm
164 N2914842d30a44824bab0e7259fdf9d3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Immunoenzyme Techniques
166 rdf:type schema:DefinedTerm
167 N2ae395bcb18b48d0a7dbe68a4d307f03 schema:name dimensions_id
168 schema:value pub.1011437644
169 rdf:type schema:PropertyValue
170 N314aa43f27224ed28a3932e72c944542 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Middle Aged
172 rdf:type schema:DefinedTerm
173 N32170f6d93e84a8888a38992f6b03966 rdf:first sg:person.01054077577.24
174 rdf:rest Nfa75bdfe8d2f49439cfff65aaac73f17
175 N321810d1355449b69244426d17a473cb rdf:first sg:person.0773025611.35
176 rdf:rest rdf:nil
177 N42cd79931966424ebf4271e1d46ad684 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Adenocarcinoma
179 rdf:type schema:DefinedTerm
180 N5538ff296a784c28b05bae1ff8421474 rdf:first sg:person.01245062664.63
181 rdf:rest N32170f6d93e84a8888a38992f6b03966
182 N65cc4850a8df4731b42200aada9cd051 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Carcinoma, Pancreatic Ductal
184 rdf:type schema:DefinedTerm
185 N66ca5d8253874f0b80a19490b95a514d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Male
187 rdf:type schema:DefinedTerm
188 N7068b3f184694c7ea83db5b700b50d23 rdf:first sg:person.01371003243.15
189 rdf:rest Na67335bd6c43414abb903270c49ef1f9
190 N72a1d8a4de074f039d6874479566e0f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Prognosis
192 rdf:type schema:DefinedTerm
193 N72afb4feab4b46c69980e4b3a9746b6c rdf:first sg:person.016423226454.74
194 rdf:rest Nf9aa389908064d0c9407b199b3a4a32d
195 N77703b91a21c4dafa24b1ed87a19f7bf schema:name doi
196 schema:value 10.1245/s10434-008-0061-5
197 rdf:type schema:PropertyValue
198 N83844272d28741fe9ba0a6831f5380c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Blotting, Western
200 rdf:type schema:DefinedTerm
201 N8a8897b13bf6450683d61db53ccceeea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
202 schema:name Female
203 rdf:type schema:DefinedTerm
204 N8f6eb3737b78411096248eeb4c38c49c rdf:first sg:person.01266546053.57
205 rdf:rest Nbd2a94bc349942f4a228e347c4221a58
206 N8fde297cb6a24ef290d3679e26479ef5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name Neoplasm Recurrence, Local
208 rdf:type schema:DefinedTerm
209 N9aefd8db35414c3ea724650c9a7ea21d schema:name Springer Nature - SN SciGraph project
210 rdf:type schema:Organization
211 N9b832ef6f6024a17b9c7923d46cebc89 rdf:first sg:person.01354163205.16
212 rdf:rest N7068b3f184694c7ea83db5b700b50d23
213 Na554318f88cf47c38b693a7030bf1caa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
214 schema:name Gene Expression Regulation, Neoplastic
215 rdf:type schema:DefinedTerm
216 Na67335bd6c43414abb903270c49ef1f9 rdf:first sg:person.0723474205.07
217 rdf:rest N321810d1355449b69244426d17a473cb
218 Nb1cb91a0016e44c091d72324cbdaa901 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
219 schema:name Drug Resistance, Neoplasm
220 rdf:type schema:DefinedTerm
221 Nbbdc2f47d0854d5abb28dae854b95356 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
222 schema:name Annexin A2
223 rdf:type schema:DefinedTerm
224 Nbd2a94bc349942f4a228e347c4221a58 rdf:first sg:person.0660326574.22
225 rdf:rest N5538ff296a784c28b05bae1ff8421474
226 Nbdf0a8178a594a48a34746117abf09e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
227 schema:name RNA, Messenger
228 rdf:type schema:DefinedTerm
229 Ncb9737cc37b1447d92335390158bf5e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
230 schema:name Carcinoma, Adenosquamous
231 rdf:type schema:DefinedTerm
232 Ncc20f618cb144fbe8c71d9c09916cedc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
233 schema:name Tumor Cells, Cultured
234 rdf:type schema:DefinedTerm
235 Ne9559ac712b148f78a60939163536d9b schema:volumeNumber 15
236 rdf:type schema:PublicationVolume
237 Ned33daf1c1be4fe8a8d0a321ca799f60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
238 schema:name Neoplasm Invasiveness
239 rdf:type schema:DefinedTerm
240 Nf61d69aab12b461fbb17df76e5d33d65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
241 schema:name Deoxycytidine
242 rdf:type schema:DefinedTerm
243 Nf9aa389908064d0c9407b199b3a4a32d rdf:first sg:person.0653527253.30
244 rdf:rest N9b832ef6f6024a17b9c7923d46cebc89
245 Nfa75bdfe8d2f49439cfff65aaac73f17 rdf:first sg:person.01127754745.32
246 rdf:rest N004cf3c5b16341dab43278c040df2f5c
247 Nfd57afe98dcc4a8e99ad203e877f943f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
248 schema:name Chemotherapy, Adjuvant
249 rdf:type schema:DefinedTerm
250 Nff2251c9f3824f7aa29bbd85c97ffe50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
251 schema:name Aged
252 rdf:type schema:DefinedTerm
253 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
254 schema:name Medical and Health Sciences
255 rdf:type schema:DefinedTerm
256 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
257 schema:name Oncology and Carcinogenesis
258 rdf:type schema:DefinedTerm
259 sg:journal.1105545 schema:issn 1068-9265
260 1534-4681
261 schema:name Annals of Surgical Oncology
262 schema:publisher Springer Nature
263 rdf:type schema:Periodical
264 sg:person.01054077577.24 schema:affiliation grid-institutes:grid.136304.3
265 schema:familyName Shida
266 schema:givenName Takashi
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054077577.24
268 rdf:type schema:Person
269 sg:person.01103413111.45 schema:affiliation grid-institutes:grid.136304.3
270 schema:familyName Shimizu
271 schema:givenName Hiroaki
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103413111.45
273 rdf:type schema:Person
274 sg:person.01127754745.32 schema:affiliation grid-institutes:grid.136304.3
275 schema:familyName Kimura
276 schema:givenName Fumio
277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127754745.32
278 rdf:type schema:Person
279 sg:person.01245062664.63 schema:affiliation grid-institutes:grid.136304.3
280 schema:familyName Yoshitomi
281 schema:givenName Hideyuki
282 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245062664.63
283 rdf:type schema:Person
284 sg:person.01266546053.57 schema:affiliation grid-institutes:grid.136304.3
285 schema:familyName Takano
286 schema:givenName Shigetsugu
287 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266546053.57
288 rdf:type schema:Person
289 sg:person.01354163205.16 schema:affiliation grid-institutes:grid.136304.3
290 schema:familyName Kato
291 schema:givenName Atsushi
292 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354163205.16
293 rdf:type schema:Person
294 sg:person.01371003243.15 schema:affiliation grid-institutes:grid.136304.3
295 schema:familyName Tomonaga
296 schema:givenName Takeshi
297 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371003243.15
298 rdf:type schema:Person
299 sg:person.016423226454.74 schema:affiliation grid-institutes:grid.136304.3
300 schema:familyName Yoshidome
301 schema:givenName Hiroyuki
302 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016423226454.74
303 rdf:type schema:Person
304 sg:person.0653527253.30 schema:affiliation grid-institutes:grid.136304.3
305 schema:familyName Ohtsuka
306 schema:givenName Masayuki
307 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653527253.30
308 rdf:type schema:Person
309 sg:person.0660326574.22 schema:affiliation grid-institutes:grid.136304.3
310 schema:familyName Togawa
311 schema:givenName Akira
312 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660326574.22
313 rdf:type schema:Person
314 sg:person.0723474205.07 schema:affiliation grid-institutes:grid.136304.3
315 schema:familyName Nomura
316 schema:givenName Fumio
317 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723474205.07
318 rdf:type schema:Person
319 sg:person.0773025611.35 schema:affiliation grid-institutes:grid.136304.3
320 schema:familyName Miyazaki
321 schema:givenName Masaru
322 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773025611.35
323 rdf:type schema:Person
324 sg:pub.10.1007/s11010-007-9649-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022402190
325 https://doi.org/10.1007/s11010-007-9649-5
326 rdf:type schema:CreativeWork
327 sg:pub.10.1023/a:1005953000523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009957264
328 https://doi.org/10.1023/a:1005953000523
329 rdf:type schema:CreativeWork
330 sg:pub.10.1038/bjc.1992.103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044981451
331 https://doi.org/10.1038/bjc.1992.103
332 rdf:type schema:CreativeWork
333 sg:pub.10.1038/bjc.1998.750 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002720064
334 https://doi.org/10.1038/bjc.1998.750
335 rdf:type schema:CreativeWork
336 sg:pub.10.1038/nrc949 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009614296
337 https://doi.org/10.1038/nrc949
338 rdf:type schema:CreativeWork
339 sg:pub.10.1038/sj.onc.1210951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019746269
340 https://doi.org/10.1038/sj.onc.1210951
341 rdf:type schema:CreativeWork
342 grid-institutes:grid.136304.3 schema:alternateName Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan
343 The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan
344 schema:name Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan
345 The Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan
346 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...