Polyunsaturated Fatty Acid Supplementation Alters Proinflammatory Gene Expression and Reduces the Incidence of Necrotizing Enterocolitis in a Neonatal Rat Model View Full Text


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Article Info

DATE

2007-04

AUTHORS

Jing Lu, Tamas Jilling, Dan Li, Michael S Caplan

ABSTRACT

Although supplementation of preterm formula with polyunsaturated fatty acids (PUFA) has been shown to reduce the incidence of necrotizing enterocolitis (NEC) in animal models and clinical trials, the mechanisms remain elusive. We hypothesized that the protective effect of PUFA on NEC may be due to the ability of PUFA to suppress Toll-like receptor (TLR) 4 and platelet-activating factor receptor (PAFR) gene expression (molecules that are important in the pathogenesis of NEC) in epithelial cells. To investigate the efficacy of different PUFA preparations on NEC in a neonatal rat model, we compared the incidence of NEC among the four PUFA supplemented groups--A: arachidonic acid and docosahexaenoic acid (AA+DHA), B: egg phospholipids (EP), C: DHA, and D: control without PUFA. PUFA supplementation reduced the incidence of NEC and inhibited intestinal PAFR and TLR4 gene expression compared with the controls. To validate the in vivo observations, IEC-6 cells were exposed to PAF after pretreatment with AA or DHA. Both AA and DHA supplementation blocked PAF-induced TLR4 and PAFR mRNA expression in these enterocytes. These results suggest that PUFA modulates gene expression of key factors involved in experimental NEC pathogenesis. These effects might in part explain the protective effect of PUFA on neonatal NEC. More... »

PAGES

427-432

References to SciGraph publications

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  • 1997-07. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity in NATURE
  • 1996-05. Visual Acuity and Fatty Acid Status of Term Infants Fed Human Milk and Formulas with and without Docosahexaenoate and Arachidonate from Egg Yolk Lecithin1 in PEDIATRIC RESEARCH
  • 1997-12. The Role of Recombinant Platelet-Activating Factor Acetylhydrolase in a Neonatal Rat Model of Necrotizing Enterocolitis in PEDIATRIC RESEARCH
  • 2001-05. Effect of Polyunsaturated Fatty Acid (PUFA) Supplementation on Intestinal Inflammation and Necrotizing Enterocolitis (NEC) in a Neonatal Rat Model in PEDIATRIC RESEARCH
  • 2005-10. Inhibition of ligand binding to g protein-coupled receptors by arachidonic acid in JOURNAL OF MOLECULAR NEUROSCIENCE
  • 1995-03. Endotoxin-resistant mice are protected from PAF-induced bowel injury and death role of TNF, complement activation, and endogenous PAF production in DIGESTIVE DISEASES AND SCIENCES
  • 2004-04. Intestinal Epithelial Apoptosis Initiates Gross Bowel Necrosis in an Experimental Rat Model of Neonatal Necrotizing Enterocolitis in PEDIATRIC RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1203/pdr.0b013e3180332ca5

    DOI

    http://dx.doi.org/10.1203/pdr.0b013e3180332ca5

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17515866


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