Factors Affecting the Binding of Bilirubin to Serum Albumins: Validation and Application of the Peroxidase Method View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-10-25

AUTHORS

LESLYE ROCA, SEBASTIÁN CALLIGARIS, RICHARD P. WENNBERG, CHARLES E. AHLFORS, SAFARINA G. MALIK, J DONALD OSTROW, CLAUDIO TIRIBELLI

ABSTRACT

The unbound "free" bilirubin concentration (Bf), not the total bilirubin concentration, is the critical determinant of cellular uptake and toxicity of bilirubin. We compared Bf measured by a modified peroxidase method with published data obtained with ultrafiltration and examined conditions that affect the affinity (KF) of human (HSA) and bovine (BSA) serum albumin for bilirubin. The peroxidase and ultrafiltration methods yielded similar KF values that decreased with increasing HSA concentration and the presence of 50 mM chloride. When related to ionic strength, inhibition of BSA-bilirubin binding by chloride, bromide, and sulfate were similar, whereas phosphate buffer had a smaller effect. KF was lower at 37 degrees C than at 25 degrees C for HSA but not for BSA. KF for BSA was similar at pH 7.4 and 8.0. BSA and FCS had similar binding properties. The close agreement of Bf and KF values determined by the peroxidase method with published results obtained by ultrafiltration validates both methods and supports the use of the peroxidase method as a practical technique for measuring Bf under steady state conditions in minimally diluted serum or culture medium. More... »

PAGES

724-728

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/01.pdr.0000245992.89965.94

DOI

http://dx.doi.org/10.1203/01.pdr.0000245992.89965.94

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034176787

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17065581


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bilirubin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bromides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cattle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chlorides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mathematics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peroxidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serum Albumin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sulfates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ultrafiltration", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "ROCA", 
        "givenName": "LESLYE", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "CALLIGARIS", 
        "givenName": "SEBASTI\u00c1N", 
        "id": "sg:person.01361423417.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361423417.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "WENNBERG", 
        "givenName": "RICHARD P.", 
        "id": "sg:person.070063300.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.070063300.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "AHLFORS", 
        "givenName": "CHARLES E.", 
        "id": "sg:person.01163172241.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163172241.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "MALIK", 
        "givenName": "SAFARINA G.", 
        "id": "sg:person.0746076221.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746076221.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "OSTROW", 
        "givenName": "J DONALD", 
        "id": "sg:person.01011401073.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011401073.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia", 
          "id": "http://www.grid.ac/institutes/grid.418754.b", 
          "name": [
            "Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "TIRIBELLI", 
        "givenName": "CLAUDIO", 
        "id": "sg:person.01335453021.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335453021.77"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1203/00006450-197412000-00010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024451456", 
          "https://doi.org/10.1203/00006450-197412000-00010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1203/00006450-198804000-00021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049844825", 
          "https://doi.org/10.1203/00006450-198804000-00021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1203/01.pdr.0000067486.79854.d5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013216643", 
          "https://doi.org/10.1203/01.pdr.0000067486.79854.d5"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-10-25", 
    "datePublishedReg": "2006-10-25", 
    "description": "The unbound \"free\" bilirubin concentration (Bf), not the total bilirubin concentration, is the critical determinant of cellular uptake and toxicity of bilirubin. We compared Bf measured by a modified peroxidase method with published data obtained with ultrafiltration and examined conditions that affect the affinity (KF) of human (HSA) and bovine (BSA) serum albumin for bilirubin. The peroxidase and ultrafiltration methods yielded similar KF values that decreased with increasing HSA concentration and the presence of 50 mM chloride. When related to ionic strength, inhibition of BSA-bilirubin binding by chloride, bromide, and sulfate were similar, whereas phosphate buffer had a smaller effect. KF was lower at 37 degrees C than at 25 degrees C for HSA but not for BSA. KF for BSA was similar at pH 7.4 and 8.0. BSA and FCS had similar binding properties. The close agreement of Bf and KF values determined by the peroxidase method with published results obtained by ultrafiltration validates both methods and supports the use of the peroxidase method as a practical technique for measuring Bf under steady state conditions in minimally diluted serum or culture medium.", 
    "genre": "article", 
    "id": "sg:pub.10.1203/01.pdr.0000245992.89965.94", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5150321", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1007352", 
        "issn": [
          "0031-3998", 
          "1530-0447"
        ], 
        "name": "Pediatric Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "60"
      }
    ], 
    "keywords": [
      "peroxidase method", 
      "bilirubin concentration", 
      "total bilirubin concentration", 
      "toxicity of bilirubin", 
      "bilirubin", 
      "serum albumin", 
      "binding of bilirubin", 
      "critical determinant", 
      "degrees C", 
      "cellular uptake", 
      "affinity of human", 
      "similar binding properties", 
      "albumin", 
      "culture medium", 
      "BF", 
      "ultrafiltration method", 
      "serum", 
      "concentration", 
      "toxicity", 
      "bovine serum albumin", 
      "mM chloride", 
      "inhibition", 
      "BSA", 
      "small effect", 
      "humans", 
      "HSA concentration", 
      "determinants", 
      "uptake", 
      "binding properties", 
      "peroxidase", 
      "FCS", 
      "factors", 
      "effect", 
      "HSA", 
      "binding", 
      "unbound", 
      "presence", 
      "method", 
      "affinity", 
      "values", 
      "use", 
      "data", 
      "ultrafiltration", 
      "conditions", 
      "sulfate", 
      "phosphate buffer", 
      "chloride", 
      "steady-state conditions", 
      "validation", 
      "results", 
      "practical technique", 
      "technique", 
      "medium", 
      "bromide", 
      "buffer", 
      "KF", 
      "validate", 
      "state conditions", 
      "strength", 
      "agreement", 
      "properties", 
      "applications", 
      "close agreement", 
      "Kf values", 
      "ionic strength"
    ], 
    "name": "Factors Affecting the Binding of Bilirubin to Serum Albumins: Validation and Application of the Peroxidase Method", 
    "pagination": "724-728", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034176787"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1203/01.pdr.0000245992.89965.94"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17065581"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1203/01.pdr.0000245992.89965.94", 
      "https://app.dimensions.ai/details/publication/pub.1034176787"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:23", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_424.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1203/01.pdr.0000245992.89965.94"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1203/01.pdr.0000245992.89965.94'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1203/01.pdr.0000245992.89965.94'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1203/01.pdr.0000245992.89965.94'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1203/01.pdr.0000245992.89965.94'


 

This table displays all metadata directly associated to this object as RDF triples.

246 TRIPLES      22 PREDICATES      110 URIs      99 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1203/01.pdr.0000245992.89965.94 schema:about N0090e7f4443b4ecfaec1e87822a6f02f
2 N0795cfa8bc8f4b02857ab2f939cdfe79
3 N12a7a9cf457243fbad7bacd54d97f7e9
4 N3146093d6e9f47ad9f55108fde79480a
5 N35a031e1dbd24c6dbf3f56b2e3225a8d
6 N37e0678d76324f80a8efe10f7d935d7d
7 N3c7750a97193400e936a8ea04b6f1aef
8 N3d1850dcc9764291ab92f7de5fb450d9
9 N419f1be44ca34c4eb0bd339bd8a2b30a
10 N664412d6da1d446db09149a025b66bda
11 N753da0dfafda4ed9874aa2da640e44c2
12 N78d8989e405846a688817b6a74902a0e
13 N7fac18b31b00460fad9331fd480099b2
14 Nd3f2312eb2504040b772eb73e4a18c54
15 Nfc4f81632d6d452197152b26d1971fd1
16 Nfc5e43950eb943d9a67e2dc1ceca1164
17 anzsrc-for:11
18 anzsrc-for:1103
19 schema:author Nf3ebcade92b6411c9e59b3676b4425e5
20 schema:citation sg:pub.10.1203/00006450-197412000-00010
21 sg:pub.10.1203/00006450-198804000-00021
22 sg:pub.10.1203/01.pdr.0000067486.79854.d5
23 schema:datePublished 2006-10-25
24 schema:datePublishedReg 2006-10-25
25 schema:description The unbound "free" bilirubin concentration (Bf), not the total bilirubin concentration, is the critical determinant of cellular uptake and toxicity of bilirubin. We compared Bf measured by a modified peroxidase method with published data obtained with ultrafiltration and examined conditions that affect the affinity (KF) of human (HSA) and bovine (BSA) serum albumin for bilirubin. The peroxidase and ultrafiltration methods yielded similar KF values that decreased with increasing HSA concentration and the presence of 50 mM chloride. When related to ionic strength, inhibition of BSA-bilirubin binding by chloride, bromide, and sulfate were similar, whereas phosphate buffer had a smaller effect. KF was lower at 37 degrees C than at 25 degrees C for HSA but not for BSA. KF for BSA was similar at pH 7.4 and 8.0. BSA and FCS had similar binding properties. The close agreement of Bf and KF values determined by the peroxidase method with published results obtained by ultrafiltration validates both methods and supports the use of the peroxidase method as a practical technique for measuring Bf under steady state conditions in minimally diluted serum or culture medium.
26 schema:genre article
27 schema:inLanguage en
28 schema:isAccessibleForFree true
29 schema:isPartOf N175a587cc6ae4d4b87880da09f76b623
30 Ncd65d73c76da4f28a22dcf59f1012899
31 sg:journal.1007352
32 schema:keywords BF
33 BSA
34 FCS
35 HSA
36 HSA concentration
37 KF
38 Kf values
39 affinity
40 affinity of human
41 agreement
42 albumin
43 applications
44 bilirubin
45 bilirubin concentration
46 binding
47 binding of bilirubin
48 binding properties
49 bovine serum albumin
50 bromide
51 buffer
52 cellular uptake
53 chloride
54 close agreement
55 concentration
56 conditions
57 critical determinant
58 culture medium
59 data
60 degrees C
61 determinants
62 effect
63 factors
64 humans
65 inhibition
66 ionic strength
67 mM chloride
68 medium
69 method
70 peroxidase
71 peroxidase method
72 phosphate buffer
73 practical technique
74 presence
75 properties
76 results
77 serum
78 serum albumin
79 similar binding properties
80 small effect
81 state conditions
82 steady-state conditions
83 strength
84 sulfate
85 technique
86 total bilirubin concentration
87 toxicity
88 toxicity of bilirubin
89 ultrafiltration
90 ultrafiltration method
91 unbound
92 uptake
93 use
94 validate
95 validation
96 values
97 schema:name Factors Affecting the Binding of Bilirubin to Serum Albumins: Validation and Application of the Peroxidase Method
98 schema:pagination 724-728
99 schema:productId N17e6d2f807bc42f4bddce7864b607aa5
100 N66ddcb6e23d74fc384550bb66c7c186a
101 Na61d2f4b7ce842ce8e22198c3130d41e
102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034176787
103 https://doi.org/10.1203/01.pdr.0000245992.89965.94
104 schema:sdDatePublished 2022-05-20T07:23
105 schema:sdLicense https://scigraph.springernature.com/explorer/license/
106 schema:sdPublisher N85af43eb945341c4bc485662d096e48c
107 schema:url https://doi.org/10.1203/01.pdr.0000245992.89965.94
108 sgo:license sg:explorer/license/
109 sgo:sdDataset articles
110 rdf:type schema:ScholarlyArticle
111 N0090e7f4443b4ecfaec1e87822a6f02f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Animals
113 rdf:type schema:DefinedTerm
114 N0795cfa8bc8f4b02857ab2f939cdfe79 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Protein Binding
116 rdf:type schema:DefinedTerm
117 N12a7a9cf457243fbad7bacd54d97f7e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Ultrafiltration
119 rdf:type schema:DefinedTerm
120 N175a587cc6ae4d4b87880da09f76b623 schema:volumeNumber 60
121 rdf:type schema:PublicationVolume
122 N17e6d2f807bc42f4bddce7864b607aa5 schema:name dimensions_id
123 schema:value pub.1034176787
124 rdf:type schema:PropertyValue
125 N3146093d6e9f47ad9f55108fde79480a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Bilirubin
127 rdf:type schema:DefinedTerm
128 N35a031e1dbd24c6dbf3f56b2e3225a8d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Temperature
130 rdf:type schema:DefinedTerm
131 N37e0678d76324f80a8efe10f7d935d7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Sulfates
133 rdf:type schema:DefinedTerm
134 N3c7750a97193400e936a8ea04b6f1aef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Chlorides
136 rdf:type schema:DefinedTerm
137 N3d1850dcc9764291ab92f7de5fb450d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Humans
139 rdf:type schema:DefinedTerm
140 N3d25ee81ac2f4771bb3f8341fe0cd969 rdf:first sg:person.0746076221.50
141 rdf:rest Nd360a79654f545df87a82a0dac3c472e
142 N419f1be44ca34c4eb0bd339bd8a2b30a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Tissue Culture Techniques
144 rdf:type schema:DefinedTerm
145 N60ef98ca204e405ba8b15b59c10eed43 rdf:first sg:person.01163172241.29
146 rdf:rest N3d25ee81ac2f4771bb3f8341fe0cd969
147 N664412d6da1d446db09149a025b66bda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Mathematics
149 rdf:type schema:DefinedTerm
150 N66ddcb6e23d74fc384550bb66c7c186a schema:name pubmed_id
151 schema:value 17065581
152 rdf:type schema:PropertyValue
153 N6c3e53a98e4040a08c6d6e8def190af9 rdf:first sg:person.070063300.96
154 rdf:rest N60ef98ca204e405ba8b15b59c10eed43
155 N753da0dfafda4ed9874aa2da640e44c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Serum Albumin
157 rdf:type schema:DefinedTerm
158 N78d8989e405846a688817b6a74902a0e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Hydrogen-Ion Concentration
160 rdf:type schema:DefinedTerm
161 N7de8572aaa154ffbb991893fce2d2078 rdf:first sg:person.01335453021.77
162 rdf:rest rdf:nil
163 N7fac18b31b00460fad9331fd480099b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Bromides
165 rdf:type schema:DefinedTerm
166 N85af43eb945341c4bc485662d096e48c schema:name Springer Nature - SN SciGraph project
167 rdf:type schema:Organization
168 Na61d2f4b7ce842ce8e22198c3130d41e schema:name doi
169 schema:value 10.1203/01.pdr.0000245992.89965.94
170 rdf:type schema:PropertyValue
171 Nca29fff2e43c4ad0aae26d1be09be769 schema:affiliation grid-institutes:grid.418754.b
172 schema:familyName ROCA
173 schema:givenName LESLYE
174 rdf:type schema:Person
175 Ncd14f2494c3e4a0ca5f38f2bfa4c7f7b rdf:first sg:person.01361423417.07
176 rdf:rest N6c3e53a98e4040a08c6d6e8def190af9
177 Ncd65d73c76da4f28a22dcf59f1012899 schema:issueNumber 6
178 rdf:type schema:PublicationIssue
179 Nd360a79654f545df87a82a0dac3c472e rdf:first sg:person.01011401073.67
180 rdf:rest N7de8572aaa154ffbb991893fce2d2078
181 Nd3f2312eb2504040b772eb73e4a18c54 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Culture Media
183 rdf:type schema:DefinedTerm
184 Nf3ebcade92b6411c9e59b3676b4425e5 rdf:first Nca29fff2e43c4ad0aae26d1be09be769
185 rdf:rest Ncd14f2494c3e4a0ca5f38f2bfa4c7f7b
186 Nfc4f81632d6d452197152b26d1971fd1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Peroxidase
188 rdf:type schema:DefinedTerm
189 Nfc5e43950eb943d9a67e2dc1ceca1164 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Cattle
191 rdf:type schema:DefinedTerm
192 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
193 schema:name Medical and Health Sciences
194 rdf:type schema:DefinedTerm
195 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
196 schema:name Clinical Sciences
197 rdf:type schema:DefinedTerm
198 sg:grant.5150321 http://pending.schema.org/fundedItem sg:pub.10.1203/01.pdr.0000245992.89965.94
199 rdf:type schema:MonetaryGrant
200 sg:journal.1007352 schema:issn 0031-3998
201 1530-0447
202 schema:name Pediatric Research
203 schema:publisher Springer Nature
204 rdf:type schema:Periodical
205 sg:person.01011401073.67 schema:affiliation grid-institutes:grid.418754.b
206 schema:familyName OSTROW
207 schema:givenName J DONALD
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011401073.67
209 rdf:type schema:Person
210 sg:person.01163172241.29 schema:affiliation grid-institutes:grid.418754.b
211 schema:familyName AHLFORS
212 schema:givenName CHARLES E.
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163172241.29
214 rdf:type schema:Person
215 sg:person.01335453021.77 schema:affiliation grid-institutes:grid.418754.b
216 schema:familyName TIRIBELLI
217 schema:givenName CLAUDIO
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335453021.77
219 rdf:type schema:Person
220 sg:person.01361423417.07 schema:affiliation grid-institutes:grid.418754.b
221 schema:familyName CALLIGARIS
222 schema:givenName SEBASTIÁN
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361423417.07
224 rdf:type schema:Person
225 sg:person.070063300.96 schema:affiliation grid-institutes:grid.418754.b
226 schema:familyName WENNBERG
227 schema:givenName RICHARD P.
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.070063300.96
229 rdf:type schema:Person
230 sg:person.0746076221.50 schema:affiliation grid-institutes:grid.418754.b
231 schema:familyName MALIK
232 schema:givenName SAFARINA G.
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746076221.50
234 rdf:type schema:Person
235 sg:pub.10.1203/00006450-197412000-00010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024451456
236 https://doi.org/10.1203/00006450-197412000-00010
237 rdf:type schema:CreativeWork
238 sg:pub.10.1203/00006450-198804000-00021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049844825
239 https://doi.org/10.1203/00006450-198804000-00021
240 rdf:type schema:CreativeWork
241 sg:pub.10.1203/01.pdr.0000067486.79854.d5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013216643
242 https://doi.org/10.1203/01.pdr.0000067486.79854.d5
243 rdf:type schema:CreativeWork
244 grid-institutes:grid.418754.b schema:alternateName Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia
245 schema:name Centro Studi Fegato, Department of Biochima, Biofisica Chimica, Marcomolecole [L.R., S.C., C.T.], AREA Science Park, University of Trieste, 34012 Trieste, Italy; Department of Pediatrics [R.P.W.], Department of Medicine [J.D.O.], University of Washington, Seattle, WA, 98195; Vashon, WA, 98070 [C.E.A]; Eijkman Institute for Molecular Biology [S.G.M.], Jakarta 10430, Indonesia
246 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...