51 Effect Of Cyclosporin A (Csa) And Verapamil On Membrane Fluidity Of Cultured Human Skin Fibroblasts (Hsf) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1990-09

AUTHORS

Vytautas Batchiulis, Hermann Toplak, Ulrich Wiesmann

ABSTRACT

Calcium entry blockade is nephroprotective in the case of CSA toxicity. Therefore we studied the effect of CSA and verapamil on membrane fluidity of cultured human skin fibroblasts. Fluorescence anisotropy (rG), inversely related to membrane fluidity, was measured in Hank's solution in the presence of, CSA (250, 750, 1500, 10000 ng/ml) and verapamil (10−6 10−5, 10−4M) at 37°C using the fluorescent marker 1, 6-diphenyl-1, 3, 5-hexatriene. CSA evoked a dose dependent decrease of rG from 0,103±0,006 to 0,090±0,008, when compared to control (0,132±0,011). At the concentration of 10−6M verapamil increased rG from 0,173±0,011 to 0,198±0,012. High concentration of verapamil (10−4M) decreased rG from 0,173±0,011 to 0,138±0,005. The therapeutic concentration of verapamil has stabilizing effect on cell membrane. It might be assumed that verapamil reduces CSA toxicity by modifying cell membrane fluidity. More... »

PAGES

285

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-199009000-00075

DOI

http://dx.doi.org/10.1203/00006450-199009000-00075

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036354676


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