Interleukin 1B Production By Colonic Mucosa Children With Crohn'S Disease View Full Text


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Article Info

DATE

1989-09

AUTHORS

S Hill, P J Milla, R Mirakian

ABSTRACT

Intereukin 18 (IL-1) is a mediator of many of the biological activities important in inflammation. It is still controversial whether there is increased activity of IL-1 in Crohn's disease (CD). Mucosal biopsies taken at colonoscopy from 5 children with CD and 5 control patients were studied. All children had presented with chronic diarrhoea, and in all 5 with CD a histological diagnosis had been made. 3 of the 5 were on immunosuppressive treatment with Prednisolone. The colonic histology was normal in 3 control children, but 2 had a mild non-specific colitis (NSC). 3 colonic biopsies were taken from each child, 2 was snap frozen and stored and 2 were cultured for 24 hours in 2 mls of standard culture medium. After 2 hours, LPS (50 μg/ml) was added to one of the biopsies. After 24 hours the biopsies and supernatants were havested and stored. An ELISA assay, with a sensitivity of 400-1000 μg/ml was used to measure the concentration of IL-1β in the supernatant. In supernatants from normal controls no IL-1B was detected but in 3/5 with CD it was present (0.05, 1.42, 1.7 μg/ml) and in 1 with NSC (1.1). Following stimulation with LPS, IL-1B was present in all with CD and 1 with NSC. In 3/5 with CD it rose markedly (0.2 - 0.8, 0.4 - 1.6, 0.5 - 1.4). In conclusion 1L-1B secretion was detected in CD and NSC. The phenomenon was enhanced by LPS and may not be specific to the inflammation of CD. More... »

PAGES

275

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-198909000-00072

DOI

http://dx.doi.org/10.1203/00006450-198909000-00072

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051414161


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