60 Response Of Prostaglandin (Pg) Synthesis And Membrane Fluidity To Changes Of Osmolality In Cultured Human Skin Fibroblasts (Hsf) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1988-08

AUTHORS

Vytautas Batchiulis, Hermann Toplak, Christa Lüthv, Ulrich Wiesmann, Oskar Oetliker

ABSTRACT

The effect of changing osmolality on basal and bradykinin (BK) stimulated 6-oxo-PGFIoc, PGE2, TxB2 and arachidonic acid (AA) synthesis of human skin fibroblasts was studied, In parallel, its influence on membrane fluidity was determined using 1-(4-trimethylammoniumphenyl)-6-phenyl-1, 3, 5 -hexatriene (TMA-DPH) as a marker of superficial cell membrane layers. Hypoosmolar mannitol (35 mosm/kg) onhanced basal 6-oxoPGF1α (152%), PGE2 (292%), TxB2 (181%), AA (549%) and BK stimulated 6-oxoPGF1cx (194%), PGE2 (342%), TxB2 (152%) and AA (402%) production, when compared to isotonic control (100%). rG (fluorescence anisotropy, inversely related to membrane fluidity) was significantly decreased from 0.362±0.009 to 0.281±0.024 (p < 0.001). In hyperosmolar mannitol (610 mosm/kg) the basal and BK simulated PG synthesis was either unaltered or inhibited (in BK stimulated release PGE2 was 77% and AA 70%). rG was slightly increased from 0.362±0.009 to 0.368±0.026 (p > 0.05), when compared to isotonic mannitol. More... »

PAGES

270

Journal

TITLE

Pediatric Research

ISSUE

2

VOLUME

24

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-198808000-00086

DOI

http://dx.doi.org/10.1203/00006450-198808000-00086

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009512258


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