Developmental Changes Of Latency And Udp-N-Acetylglucosa Mine (Nag) Effect On Hepatic Udp-Glucuronyltransferase (Udp-Gt) In Rat Liver Intact Microsomes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1987-08

AUTHORS

P Vajro, N Blanckaert, M M Thaler

ABSTRACT

Membrane bound-luminally oriented hepatic UDP-GT is largely unexpressed in liver intact microsomes, and is fully revealed by pretreatment with membrane perturbing agents (latency). Whether the response of UDP -GT to these agents changes during development is poorly known, besides the few existing studies having been carried out without monitoring of microsomal structural integrity. We examined the response of UDP-GT (naphtol as substrate) to a detergent (CHAPSO) and to the putative physiological cofactor NAG in developing rat liver microsomes. Phospholipid (PL) membrane content, and 2 independent parameters of membrane integrity, i.e. latency of low Km microsomal marker Man6Pase and permeability to EDTA, were also assessed.RESULTS(*p<0.05:developing vs adults)CONCLUSION We have shown in rat liver intact microsomes that response of UDP-GT to both NAG and detergent activation undergoes major changes during development. More... »

PAGES

242-242

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-198708000-00173

DOI

http://dx.doi.org/10.1203/00006450-198708000-00173

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020691603


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