Ontology type: schema:ScholarlyArticle Open Access: True
1987-04
AUTHORSJon S Abramson, Lisa F Cassidy, Douglas S Lyles
ABSTRACTPMNLs are well differentiated cells and are thought to have very little biosynthetic activity, however a few studies have shown that PMNLs can produce proteins when exposed to exogenous stimuli. Previous reports have also indicated that infection of PMNLs with IAV causes depression of metabolic and chemotactic responses, but the effect PMNLs have on the lifecycle of IAV has not been well defined. The present study was done to determine whether IAV can produce proteins and replicate within PMNLs. Early stages of virus-PMNL interaction were examined using binding assays with radiolabeled IAV and electron microscopy. Individual virus particles were taken up in coated pits and by 20 min were noted to be in endocytic vacuoles. Newly synthesized proteins were detected within PMNLs by incubating PMNLs with virus or buffer for 6 hours, labeling the PMNLs with 35S-methionine for an additional hour, and analyzing the cell lysates by gel electrophoresis and fluorography. Host proteins were produced by buffer-treated PMNLs and both host and virus specific proteins were produced by IAV-Infected PMNLs. Studies using immunofluorescent techniques combined with flow cytometry confirmed that newly synthesized viral antigens were produced by virus-infected PMNLs. Plaque assays on supernatant fluid from IAV-infected PMNLs showed that infectious progeny were not produced. These data indicate that protein production occurs in both unstimulated and IAV infected PMNLs and that IAV infection of PMNLs is abortive. More... »
PAGES308a
http://scigraph.springernature.com/pub.10.1203/00006450-198704010-00844
DOIhttp://dx.doi.org/10.1203/00006450-198704010-00844
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1029615544
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Microbiology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"familyName": "Abramson",
"givenName": "Jon S",
"id": "sg:person.0677546115.17",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677546115.17"
],
"type": "Person"
},
{
"familyName": "Cassidy",
"givenName": "Lisa F",
"id": "sg:person.0744347471.93",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744347471.93"
],
"type": "Person"
},
{
"familyName": "Lyles",
"givenName": "Douglas S",
"id": "sg:person.01347247115.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347247115.52"
],
"type": "Person"
}
],
"datePublished": "1987-04",
"datePublishedReg": "1987-04-01",
"description": "PMNLs are well differentiated cells and are thought to have very little biosynthetic activity, however a few studies have shown that PMNLs can produce proteins when exposed to exogenous stimuli. Previous reports have also indicated that infection of PMNLs with IAV causes depression of metabolic and chemotactic responses, but the effect PMNLs have on the lifecycle of IAV has not been well defined. The present study was done to determine whether IAV can produce proteins and replicate within PMNLs. Early stages of virus-PMNL interaction were examined using binding assays with radiolabeled IAV and electron microscopy. Individual virus particles were taken up in coated pits and by 20 min were noted to be in endocytic vacuoles. Newly synthesized proteins were detected within PMNLs by incubating PMNLs with virus or buffer for 6 hours, labeling the PMNLs with 35S-methionine for an additional hour, and analyzing the cell lysates by gel electrophoresis and fluorography. Host proteins were produced by buffer-treated PMNLs and both host and virus specific proteins were produced by IAV-Infected PMNLs. Studies using immunofluorescent techniques combined with flow cytometry confirmed that newly synthesized viral antigens were produced by virus-infected PMNLs. Plaque assays on supernatant fluid from IAV-infected PMNLs showed that infectious progeny were not produced. These data indicate that protein production occurs in both unstimulated and IAV infected PMNLs and that IAV infection of PMNLs is abortive.",
"genre": "non_research_article",
"id": "sg:pub.10.1203/00006450-198704010-00844",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1007352",
"issn": [
"0031-3998",
"1530-0447"
],
"name": "Pediatric Research",
"type": "Periodical"
},
{
"issueNumber": "S4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "21"
}
],
"name": "SYNTHESIS OF INFLUENZA A VIRUS (IAV) AND ENDOGENOUS PROTEINS IN POLYMORPHONUCLEAR LEUKOCYTES (PMNLs)",
"pagination": "308a",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"8d15453bff215ed097478a170ee691bd43ce1e3eb00898c7815b9d6399d9198d"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1203/00006450-198704010-00844"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1029615544"
]
}
],
"sameAs": [
"https://doi.org/10.1203/00006450-198704010-00844",
"https://app.dimensions.ai/details/publication/pub.1029615544"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T18:08",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000425.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/pr1987930"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1203/00006450-198704010-00844'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1203/00006450-198704010-00844'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1203/00006450-198704010-00844'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1203/00006450-198704010-00844'
This table displays all metadata directly associated to this object as RDF triples.
69 TRIPLES
20 PREDICATES
27 URIs
19 LITERALS
7 BLANK NODES
Subject | Predicate | Object | |
---|---|---|---|
1 | sg:pub.10.1203/00006450-198704010-00844 | schema:about | anzsrc-for:11 |
2 | ″ | ″ | anzsrc-for:1108 |
3 | ″ | schema:author | Nd6d63e04e9ba44389baca70d145b0185 |
4 | ″ | schema:datePublished | 1987-04 |
5 | ″ | schema:datePublishedReg | 1987-04-01 |
6 | ″ | schema:description | PMNLs are well differentiated cells and are thought to have very little biosynthetic activity, however a few studies have shown that PMNLs can produce proteins when exposed to exogenous stimuli. Previous reports have also indicated that infection of PMNLs with IAV causes depression of metabolic and chemotactic responses, but the effect PMNLs have on the lifecycle of IAV has not been well defined. The present study was done to determine whether IAV can produce proteins and replicate within PMNLs. Early stages of virus-PMNL interaction were examined using binding assays with radiolabeled IAV and electron microscopy. Individual virus particles were taken up in coated pits and by 20 min were noted to be in endocytic vacuoles. Newly synthesized proteins were detected within PMNLs by incubating PMNLs with virus or buffer for 6 hours, labeling the PMNLs with 35S-methionine for an additional hour, and analyzing the cell lysates by gel electrophoresis and fluorography. Host proteins were produced by buffer-treated PMNLs and both host and virus specific proteins were produced by IAV-Infected PMNLs. Studies using immunofluorescent techniques combined with flow cytometry confirmed that newly synthesized viral antigens were produced by virus-infected PMNLs. Plaque assays on supernatant fluid from IAV-infected PMNLs showed that infectious progeny were not produced. These data indicate that protein production occurs in both unstimulated and IAV infected PMNLs and that IAV infection of PMNLs is abortive. |
7 | ″ | schema:genre | non_research_article |
8 | ″ | schema:inLanguage | en |
9 | ″ | schema:isAccessibleForFree | true |
10 | ″ | schema:isPartOf | N563b6d4b1c4f4138997bb581f6ae5765 |
11 | ″ | ″ | N7722c590616145958aaa5c734744a5d1 |
12 | ″ | ″ | sg:journal.1007352 |
13 | ″ | schema:name | SYNTHESIS OF INFLUENZA A VIRUS (IAV) AND ENDOGENOUS PROTEINS IN POLYMORPHONUCLEAR LEUKOCYTES (PMNLs) |
14 | ″ | schema:pagination | 308a |
15 | ″ | schema:productId | N37100910476749629743146046622f0f |
16 | ″ | ″ | N5fd60f9cfc7d4bf1b8f44ff14dd2cd5e |
17 | ″ | ″ | N89e0f79b6bb343699c0bf76ffa18c44d |
18 | ″ | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1029615544 |
19 | ″ | ″ | https://doi.org/10.1203/00006450-198704010-00844 |
20 | ″ | schema:sdDatePublished | 2019-04-10T18:08 |
21 | ″ | schema:sdLicense | https://scigraph.springernature.com/explorer/license/ |
22 | ″ | schema:sdPublisher | N46c77d493be444e496d8f24c7836a8c9 |
23 | ″ | schema:url | https://www.nature.com/articles/pr1987930 |
24 | ″ | sgo:license | sg:explorer/license/ |
25 | ″ | sgo:sdDataset | articles |
26 | ″ | rdf:type | schema:ScholarlyArticle |
27 | N37100910476749629743146046622f0f | schema:name | doi |
28 | ″ | schema:value | 10.1203/00006450-198704010-00844 |
29 | ″ | rdf:type | schema:PropertyValue |
30 | N38c44afb19344f12a51b789a9f480e47 | rdf:first | sg:person.0744347471.93 |
31 | ″ | rdf:rest | N5e0c1a8969d9495fa054da77d82d1468 |
32 | N46c77d493be444e496d8f24c7836a8c9 | schema:name | Springer Nature - SN SciGraph project |
33 | ″ | rdf:type | schema:Organization |
34 | N563b6d4b1c4f4138997bb581f6ae5765 | schema:volumeNumber | 21 |
35 | ″ | rdf:type | schema:PublicationVolume |
36 | N5e0c1a8969d9495fa054da77d82d1468 | rdf:first | sg:person.01347247115.52 |
37 | ″ | rdf:rest | rdf:nil |
38 | N5fd60f9cfc7d4bf1b8f44ff14dd2cd5e | schema:name | readcube_id |
39 | ″ | schema:value | 8d15453bff215ed097478a170ee691bd43ce1e3eb00898c7815b9d6399d9198d |
40 | ″ | rdf:type | schema:PropertyValue |
41 | N7722c590616145958aaa5c734744a5d1 | schema:issueNumber | S4 |
42 | ″ | rdf:type | schema:PublicationIssue |
43 | N89e0f79b6bb343699c0bf76ffa18c44d | schema:name | dimensions_id |
44 | ″ | schema:value | pub.1029615544 |
45 | ″ | rdf:type | schema:PropertyValue |
46 | Nd6d63e04e9ba44389baca70d145b0185 | rdf:first | sg:person.0677546115.17 |
47 | ″ | rdf:rest | N38c44afb19344f12a51b789a9f480e47 |
48 | anzsrc-for:11 | schema:inDefinedTermSet | anzsrc-for: |
49 | ″ | schema:name | Medical and Health Sciences |
50 | ″ | rdf:type | schema:DefinedTerm |
51 | anzsrc-for:1108 | schema:inDefinedTermSet | anzsrc-for: |
52 | ″ | schema:name | Medical Microbiology |
53 | ″ | rdf:type | schema:DefinedTerm |
54 | sg:journal.1007352 | schema:issn | 0031-3998 |
55 | ″ | ″ | 1530-0447 |
56 | ″ | schema:name | Pediatric Research |
57 | ″ | rdf:type | schema:Periodical |
58 | sg:person.01347247115.52 | schema:familyName | Lyles |
59 | ″ | schema:givenName | Douglas S |
60 | ″ | schema:sameAs | https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347247115.52 |
61 | ″ | rdf:type | schema:Person |
62 | sg:person.0677546115.17 | schema:familyName | Abramson |
63 | ″ | schema:givenName | Jon S |
64 | ″ | schema:sameAs | https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677546115.17 |
65 | ″ | rdf:type | schema:Person |
66 | sg:person.0744347471.93 | schema:familyName | Cassidy |
67 | ″ | schema:givenName | Lisa F |
68 | ″ | schema:sameAs | https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744347471.93 |
69 | ″ | rdf:type | schema:Person |