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    "description": "PMNLs are well differentiated cells and are thought to have very little biosynthetic activity, however a few studies have shown that PMNLs can produce proteins when exposed to exogenous stimuli. Previous reports have also indicated that infection of PMNLs with IAV causes depression of metabolic and chemotactic responses, but the effect PMNLs have on the lifecycle of IAV has not been well defined. The present study was done to determine whether IAV can produce proteins and replicate within PMNLs. Early stages of virus-PMNL interaction were examined using binding assays with radiolabeled IAV and electron microscopy. Individual virus particles were taken up in coated pits and by 20 min were noted to be in endocytic vacuoles. Newly synthesized proteins were detected within PMNLs by incubating PMNLs with virus or buffer for 6 hours, labeling the PMNLs with 35S-methionine for an additional hour, and analyzing the cell lysates by gel electrophoresis and fluorography. Host proteins were produced by buffer-treated PMNLs and both host and virus specific proteins were produced by IAV-Infected PMNLs. Studies using immunofluorescent techniques combined with flow cytometry confirmed that newly synthesized viral antigens were produced by virus-infected PMNLs. Plaque assays on supernatant fluid from IAV-infected PMNLs showed that infectious progeny were not produced. These data indicate that protein production occurs in both unstimulated and IAV infected PMNLs and that IAV infection of PMNLs is abortive.", 
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