In Vivo Effect Of Free Fatty Acids (Ffa) On Bilirubin (Br) Binding View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1984-04

AUTHORS

Timos Valaes, Kevin Murphy, Richard Wennberg, Boris Senior

ABSTRACT

In healthy adults a wide range (0.28 to 5.34mMol/L) of plasma FFA levels were generated by the fat meal-heparin regimen (Schalch DS, Kipnis DM, J.Clin.Invest.44:2010,1965). In each experiment 4-5 blood samples were collected, albumin (Alb) and FFA concentrations were measured and BR binding assessed using: (a) BR titration of sera using Sephadex (S) Column Chromatography (Valaes T, Hyte M, Pediatrics 59:881,1977); (b) the Undiluted Peroxidase (P) method (Ahlfors CE, Clin.Chem.27:692,1981); and (c) the automated Bilirubin Hematofluorometer (HMF) (Wells R et al Clin.Chem.28:432,1982). With S a significant negative correlation was found between the BR/Alb (molar ratio) at the BR titration point ("BR binding capacity") and the FFA/Alb (Y=0.883 -0.088·X, r=-0.90, p<0.001, n=38). Similarly, after "spicing" the sera to BR/Alb of 0.367±0.029(SD) the Apparent Unbound BR (nMol/L) by the P method was significantly related to the FFA/Alb (Y=13.5±41.66.X, r=0.957, p<0.001, n=28). The BR/Alb at BR binding capacity by HMF was only weakly related to FFA/Alb (Y=0.711-0.009·X, r=-0.353, p<0.05, n=34). However, "spicing" of the blood samples to BR/Alb of 0.54±0.054(SD) improved the correlation (Y=0.929-0.041·X, r=-0.835, p<0.001, n=12). The reasons for this effect of "spicing" are not clear.Previous conclusions of no effect up to FFA/Alb of 4, derived from studies with Alb solutions and in vitro additions of FFA, should be revised. More... »

PAGES

353-353

Journal

TITLE

Pediatric Research

ISSUE

Suppl 4

VOLUME

18

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-198404001-01559

DOI

http://dx.doi.org/10.1203/00006450-198404001-01559

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007831142


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Depts. of Pediatrics", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Tufts-New England Medical Center, Boston", 
            "Univ. of California, Davis", 
            "Depts. of Pediatrics"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Valaes", 
        "givenName": "Timos", 
        "id": "sg:person.047062746.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.047062746.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Depts. of Pediatrics", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Tufts-New England Medical Center, Boston", 
            "Univ. of California, Davis", 
            "Depts. of Pediatrics"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murphy", 
        "givenName": "Kevin", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Depts. of Pediatrics", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Tufts-New England Medical Center, Boston", 
            "Univ. of California, Davis", 
            "Depts. of Pediatrics"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wennberg", 
        "givenName": "Richard", 
        "id": "sg:person.070063300.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.070063300.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Depts. of Pediatrics", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Tufts-New England Medical Center, Boston", 
            "Univ. of California, Davis", 
            "Depts. of Pediatrics"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Senior", 
        "givenName": "Boris", 
        "id": "sg:person.075045511.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.075045511.11"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1984-04", 
    "datePublishedReg": "1984-04-01", 
    "description": "In healthy adults a wide range (0.28 to 5.34mMol/L) of plasma FFA levels were generated by the fat meal-heparin regimen (Schalch DS, Kipnis DM, J.Clin.Invest.44:2010,1965). In each experiment 4-5 blood samples were collected, albumin (Alb) and FFA concentrations were measured and BR binding assessed using: (a) BR titration of sera using Sephadex (S) Column Chromatography (Valaes T, Hyte M, Pediatrics 59:881,1977); (b) the Undiluted Peroxidase (P) method (Ahlfors CE, Clin.Chem.27:692,1981); and (c) the automated Bilirubin Hematofluorometer (HMF) (Wells R et al Clin.Chem.28:432,1982). With S a significant negative correlation was found between the BR/Alb (molar ratio) at the BR titration point (\"BR binding capacity\") and the FFA/Alb (Y=0.883 -0.088\u00b7X, r=-0.90, p<0.001, n=38). Similarly, after \"spicing\" the sera to BR/Alb of 0.367\u00b10.029(SD) the Apparent Unbound BR (nMol/L) by the P method was significantly related to the FFA/Alb (Y=13.5\u00b141.66.X, r=0.957, p<0.001, n=28). The BR/Alb at BR binding capacity by HMF was only weakly related to FFA/Alb (Y=0.711-0.009\u00b7X, r=-0.353, p<0.05, n=34). However, \"spicing\" of the blood samples to BR/Alb of 0.54\u00b10.054(SD) improved the correlation (Y=0.929-0.041\u00b7X, r=-0.835, p<0.001, n=12). The reasons for this effect of \"spicing\" are not clear.Previous conclusions of no effect up to FFA/Alb of 4, derived from studies with Alb solutions and in vitro additions of FFA, should be revised.", 
    "genre": "article", 
    "id": "sg:pub.10.1203/00006450-198404001-01559", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1007352", 
        "issn": [
          "0031-3998", 
          "1530-0447"
        ], 
        "name": "Pediatric Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "Suppl 4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "keywords": [
      "free fatty acids", 
      "blood samples", 
      "plasma FFA levels", 
      "addition of FFA", 
      "FFA levels", 
      "vivo effects", 
      "FFA concentrations", 
      "healthy adults", 
      "significant negative correlation", 
      "peroxidase method", 
      "bilirubin binding", 
      "Alb", 
      "hematofluorometer", 
      "serum", 
      "fatty acids", 
      "Sephadex column chromatography", 
      "negative correlation", 
      "regimen", 
      "adults", 
      "effect", 
      "ALB solutions", 
      "albumin", 
      "correlation", 
      "conclusion", 
      "column chromatography", 
      "Experiment 4", 
      "samples", 
      "levels", 
      "study", 
      "binding", 
      "concentration", 
      "acid", 
      "spicing", 
      "previous conclusions", 
      "addition", 
      "chromatography", 
      "reasons", 
      "method", 
      "capacity", 
      "wide range", 
      "titration", 
      "point", 
      "Br", 
      "range", 
      "titration point", 
      "solution"
    ], 
    "name": "IN VIVO EFFECT OF FREE FATTY ACIDS (FFA) ON BILIRUBIN (BR) BINDING", 
    "pagination": "353-353", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1007831142"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1203/00006450-198404001-01559"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1203/00006450-198404001-01559", 
      "https://app.dimensions.ai/details/publication/pub.1007831142"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_176.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1203/00006450-198404001-01559"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1203/00006450-198404001-01559'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1203/00006450-198404001-01559'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1203/00006450-198404001-01559'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1203/00006450-198404001-01559'


 

This table displays all metadata directly associated to this object as RDF triples.

126 TRIPLES      21 PREDICATES      72 URIs      64 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1203/00006450-198404001-01559 schema:about anzsrc-for:11
2 anzsrc-for:1103
3 schema:author N0ead82bc09d4457db6626476dedf475d
4 schema:datePublished 1984-04
5 schema:datePublishedReg 1984-04-01
6 schema:description In healthy adults a wide range (0.28 to 5.34mMol/L) of plasma FFA levels were generated by the fat meal-heparin regimen (Schalch DS, Kipnis DM, J.Clin.Invest.44:2010,1965). In each experiment 4-5 blood samples were collected, albumin (Alb) and FFA concentrations were measured and BR binding assessed using: (a) BR titration of sera using Sephadex (S) Column Chromatography (Valaes T, Hyte M, Pediatrics 59:881,1977); (b) the Undiluted Peroxidase (P) method (Ahlfors CE, Clin.Chem.27:692,1981); and (c) the automated Bilirubin Hematofluorometer (HMF) (Wells R et al Clin.Chem.28:432,1982). With S a significant negative correlation was found between the BR/Alb (molar ratio) at the BR titration point ("BR binding capacity") and the FFA/Alb (Y=0.883 -0.088·X, r=-0.90, p<0.001, n=38). Similarly, after "spicing" the sera to BR/Alb of 0.367±0.029(SD) the Apparent Unbound BR (nMol/L) by the P method was significantly related to the FFA/Alb (Y=13.5±41.66.X, r=0.957, p<0.001, n=28). The BR/Alb at BR binding capacity by HMF was only weakly related to FFA/Alb (Y=0.711-0.009·X, r=-0.353, p<0.05, n=34). However, "spicing" of the blood samples to BR/Alb of 0.54±0.054(SD) improved the correlation (Y=0.929-0.041·X, r=-0.835, p<0.001, n=12). The reasons for this effect of "spicing" are not clear.Previous conclusions of no effect up to FFA/Alb of 4, derived from studies with Alb solutions and in vitro additions of FFA, should be revised.
7 schema:genre article
8 schema:inLanguage en
9 schema:isAccessibleForFree true
10 schema:isPartOf N1c49deb3a4cb4eeca173bb476c17b01c
11 N683f84efc5e044cfbfefa826ea9b8946
12 sg:journal.1007352
13 schema:keywords ALB solutions
14 Alb
15 Br
16 Experiment 4
17 FFA concentrations
18 FFA levels
19 Sephadex column chromatography
20 acid
21 addition
22 addition of FFA
23 adults
24 albumin
25 bilirubin binding
26 binding
27 blood samples
28 capacity
29 chromatography
30 column chromatography
31 concentration
32 conclusion
33 correlation
34 effect
35 fatty acids
36 free fatty acids
37 healthy adults
38 hematofluorometer
39 levels
40 method
41 negative correlation
42 peroxidase method
43 plasma FFA levels
44 point
45 previous conclusions
46 range
47 reasons
48 regimen
49 samples
50 serum
51 significant negative correlation
52 solution
53 spicing
54 study
55 titration
56 titration point
57 vivo effects
58 wide range
59 schema:name IN VIVO EFFECT OF FREE FATTY ACIDS (FFA) ON BILIRUBIN (BR) BINDING
60 schema:pagination 353-353
61 schema:productId N34ccffd0c4a54200a61b5ef6ece2d148
62 Nbe395d1b2d14479abf0c6eab6a00215d
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007831142
64 https://doi.org/10.1203/00006450-198404001-01559
65 schema:sdDatePublished 2022-05-20T07:17
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher N4b519d7299324dee815e7bbc176dd715
68 schema:url https://doi.org/10.1203/00006450-198404001-01559
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N0ead82bc09d4457db6626476dedf475d rdf:first sg:person.047062746.97
73 rdf:rest N3b4111114357432a81990fe6325c52f6
74 N1c49deb3a4cb4eeca173bb476c17b01c schema:issueNumber Suppl 4
75 rdf:type schema:PublicationIssue
76 N34ccffd0c4a54200a61b5ef6ece2d148 schema:name doi
77 schema:value 10.1203/00006450-198404001-01559
78 rdf:type schema:PropertyValue
79 N3b4111114357432a81990fe6325c52f6 rdf:first N7dd9ff0d1f5f4793a73c48ffaa773ca3
80 rdf:rest Nd0f4b3bb533e4c3bbcde9d5101d74ba8
81 N4b519d7299324dee815e7bbc176dd715 schema:name Springer Nature - SN SciGraph project
82 rdf:type schema:Organization
83 N683f84efc5e044cfbfefa826ea9b8946 schema:volumeNumber 18
84 rdf:type schema:PublicationVolume
85 N7dd9ff0d1f5f4793a73c48ffaa773ca3 schema:affiliation grid-institutes:None
86 schema:familyName Murphy
87 schema:givenName Kevin
88 rdf:type schema:Person
89 N8e9e4f70a35a4a3ba175d689f83b3ed1 rdf:first sg:person.075045511.11
90 rdf:rest rdf:nil
91 Nbe395d1b2d14479abf0c6eab6a00215d schema:name dimensions_id
92 schema:value pub.1007831142
93 rdf:type schema:PropertyValue
94 Nd0f4b3bb533e4c3bbcde9d5101d74ba8 rdf:first sg:person.070063300.96
95 rdf:rest N8e9e4f70a35a4a3ba175d689f83b3ed1
96 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
97 schema:name Medical and Health Sciences
98 rdf:type schema:DefinedTerm
99 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
100 schema:name Clinical Sciences
101 rdf:type schema:DefinedTerm
102 sg:journal.1007352 schema:issn 0031-3998
103 1530-0447
104 schema:name Pediatric Research
105 schema:publisher Springer Nature
106 rdf:type schema:Periodical
107 sg:person.047062746.97 schema:affiliation grid-institutes:None
108 schema:familyName Valaes
109 schema:givenName Timos
110 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.047062746.97
111 rdf:type schema:Person
112 sg:person.070063300.96 schema:affiliation grid-institutes:None
113 schema:familyName Wennberg
114 schema:givenName Richard
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.070063300.96
116 rdf:type schema:Person
117 sg:person.075045511.11 schema:affiliation grid-institutes:None
118 schema:familyName Senior
119 schema:givenName Boris
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.075045511.11
121 rdf:type schema:Person
122 grid-institutes:None schema:alternateName Depts. of Pediatrics
123 schema:name Depts. of Pediatrics
124 Tufts-New England Medical Center, Boston
125 Univ. of California, Davis
126 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...