Theophylline Reduces the Activity of Cerebroside-Sulfotransferase, a Key Enzyme in Myelination, in Cell Cultures from Newborn Mouse Brain View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1980-11

AUTHORS

Hans Peter Siegrist, Thomas Burkart, Karel Hoffmann, Ulrich Wiesmann, Norbert Herschkowitz

ABSTRACT

Theophylline, a drug used in neonatology for the treatment of apnea, affects cholesterol synthesis if administered in concentrations of 10(-4) M (a concentration found in serum of treated patients) for 24 hr to dissociated brain cell cultures. The rate-limiting enzyme of cholesterol synthesis, beta-hydroxy-beta-methylglutaryl-coenzyme A reductase (EC 1.1.1.34), is lowered to 45% 48 hr after removal of theophylline. At the same time, cholesterol content of the cells is lowered to 73%. Inasmuch as the phospholipid content of the cells remains stable, the treatment changes the cholesterol phospholipid ratio. Concomitant to this effect, the activity of cerebroside-sulfotransferase (EC 2.8.2.11) is lowered to 60% of control values. We postulate that these two effects are linked to each other by means of modulation of the cerebroside-sulfotransferase activity by membrane lipids. More... »

PAGES

1226

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-198011000-00015

DOI

http://dx.doi.org/10.1203/00006450-198011000-00015

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007312462

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6935624


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