1037 Factors Determining The Cellular Uptake Of Bilirubin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1978-04

AUTHORS

Richard Wennberg, L Fraser Rasmussen

ABSTRACT

The toxicity of bilirubin is a function of the amount of bilirubin bound to cellular elements and the sensitivity of the cell to a given bilirubin load. We have examined the stoichiometry of cell binding of bilirubin by incubating red blood cells with various concentrations of bilirubin and serum in vitro, measuring the serum unbound bilirubin concentration (UBC) with the peroxidase assay, and extracting the RBC bilirubin with a human serum albumin solution.Results: (1) The partitioning of the total bilirubin pool between serum and cells reached equilibrium within 10 minutes.(2) At equilibrium, the cellular content of bilirubin was a function of the UBC and not the total bilirubin concentration or bilirubin/albumin molar ratio.(3) Albumin binding of bilirubin was not affected by pH.(4) Cellular uptake of bilirubin was greatly influenced by pH. At physiological pH, bilirubin exists predominantly as an anion with only a small fraction present as the bilirubin acid. The increased bilirubin uptake at pH 7.0 vs. pH 7.4 corresponded to the calculated increase in the less soluble protonated bilirubin.Conclusions: Cellular uptake of bilirubin is a function of the concentration of free bilirubin acid. It is probable that the risk for kernicterus (with respect to blood chemistries) can be estimated by measuring the serum UBC and blood pH. More... »

PAGES

536-536

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-197804001-01043

DOI

http://dx.doi.org/10.1203/00006450-197804001-01043

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018654951


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1114", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Paediatrics and Reproductive Medicine", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1117", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Public Health and Health Services", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, Davis - Sacramento Medical Center, Sacramento, California", 
          "id": "http://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "University of California, Davis - Sacramento Medical Center, Sacramento, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wennberg", 
        "givenName": "Richard", 
        "id": "sg:person.070063300.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.070063300.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Davis - Sacramento Medical Center, Sacramento, California", 
          "id": "http://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "University of California, Davis - Sacramento Medical Center, Sacramento, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rasmussen", 
        "givenName": "L Fraser", 
        "id": "sg:person.060361100.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.060361100.77"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1978-04", 
    "datePublishedReg": "1978-04-01", 
    "description": "The toxicity of bilirubin is a function of the amount of bilirubin bound to cellular elements and the sensitivity of the cell to a given bilirubin load. We have examined the stoichiometry of cell binding of bilirubin by incubating red blood cells with various concentrations of bilirubin and serum in vitro, measuring the serum unbound bilirubin concentration (UBC) with the peroxidase assay, and extracting the RBC bilirubin with a human serum albumin solution.Results: (1) The partitioning of the total bilirubin pool between serum and cells reached equilibrium within 10 minutes.(2) At equilibrium, the cellular content of bilirubin was a function of the UBC and not the total bilirubin concentration or bilirubin/albumin molar ratio.(3) Albumin binding of bilirubin was not affected by pH.(4) Cellular uptake of bilirubin was greatly influenced by pH. At physiological pH, bilirubin exists predominantly as an anion with only a small fraction present as the bilirubin acid. The increased bilirubin uptake at pH 7.0 vs. pH 7.4 corresponded to the calculated increase in the less soluble protonated bilirubin.Conclusions: Cellular uptake of bilirubin is a function of the concentration of free bilirubin acid. It is probable that the risk for kernicterus (with respect to blood chemistries) can be estimated by measuring the serum UBC and blood pH.", 
    "genre": "article", 
    "id": "sg:pub.10.1203/00006450-197804001-01043", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1007352", 
        "issn": [
          "0031-3998", 
          "1530-0447"
        ], 
        "name": "Pediatric Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "Suppl 4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "keywords": [
      "serum unbound bilirubin concentrations", 
      "unbound bilirubin concentration", 
      "bilirubin concentration", 
      "total bilirubin concentration", 
      "bilirubin/albumin molar ratio", 
      "albumin molar ratio", 
      "bilirubin load", 
      "red blood cells", 
      "concentration of bilirubin", 
      "cellular uptake", 
      "bilirubin acid", 
      "toxicity of bilirubin", 
      "amount of bilirubin", 
      "bilirubin", 
      "blood cells", 
      "bilirubin pool", 
      "cellular elements", 
      "cell binding", 
      "albumin binding", 
      "serum", 
      "human serum albumin solution", 
      "cells", 
      "albumin solution", 
      "cellular content", 
      "peroxidase assay", 
      "uptake", 
      "kernicterus", 
      "risk", 
      "vitro", 
      "concentration", 
      "toxicity", 
      "minutes", 
      "binding", 
      "assays", 
      "function", 
      "acid", 
      "factors", 
      "sensitivity", 
      "serum albumin solution", 
      "increase", 
      "physiological pH", 
      "small fraction", 
      "pool", 
      "fraction", 
      "ratio", 
      "amount", 
      "content", 
      "pH", 
      "load", 
      "molar ratio", 
      "elements", 
      "partitioning", 
      "stoichiometry", 
      "anions", 
      "solution", 
      "equilibrium"
    ], 
    "name": "1037 FACTORS DETERMINING THE CELLULAR UPTAKE OF BILIRUBIN", 
    "pagination": "536-536", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1018654951"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1203/00006450-197804001-01043"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1203/00006450-197804001-01043", 
      "https://app.dimensions.ai/details/publication/pub.1018654951"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-10T09:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_120.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1203/00006450-197804001-01043"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1203/00006450-197804001-01043'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1203/00006450-197804001-01043'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1203/00006450-197804001-01043'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1203/00006450-197804001-01043'


 

This table displays all metadata directly associated to this object as RDF triples.

125 TRIPLES      21 PREDICATES      83 URIs      74 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1203/00006450-197804001-01043 schema:about anzsrc-for:11
2 anzsrc-for:1114
3 anzsrc-for:1117
4 schema:author N8f7ab32cf42b48f4b22b21abab08471b
5 schema:datePublished 1978-04
6 schema:datePublishedReg 1978-04-01
7 schema:description The toxicity of bilirubin is a function of the amount of bilirubin bound to cellular elements and the sensitivity of the cell to a given bilirubin load. We have examined the stoichiometry of cell binding of bilirubin by incubating red blood cells with various concentrations of bilirubin and serum in vitro, measuring the serum unbound bilirubin concentration (UBC) with the peroxidase assay, and extracting the RBC bilirubin with a human serum albumin solution.Results: (1) The partitioning of the total bilirubin pool between serum and cells reached equilibrium within 10 minutes.(2) At equilibrium, the cellular content of bilirubin was a function of the UBC and not the total bilirubin concentration or bilirubin/albumin molar ratio.(3) Albumin binding of bilirubin was not affected by pH.(4) Cellular uptake of bilirubin was greatly influenced by pH. At physiological pH, bilirubin exists predominantly as an anion with only a small fraction present as the bilirubin acid. The increased bilirubin uptake at pH 7.0 vs. pH 7.4 corresponded to the calculated increase in the less soluble protonated bilirubin.Conclusions: Cellular uptake of bilirubin is a function of the concentration of free bilirubin acid. It is probable that the risk for kernicterus (with respect to blood chemistries) can be estimated by measuring the serum UBC and blood pH.
8 schema:genre article
9 schema:inLanguage en
10 schema:isAccessibleForFree true
11 schema:isPartOf N011db4b559e8486ea9ba55ac13a23ed9
12 Nf1f325f851c94fa18ddb0d74c83402a2
13 sg:journal.1007352
14 schema:keywords acid
15 albumin binding
16 albumin molar ratio
17 albumin solution
18 amount
19 amount of bilirubin
20 anions
21 assays
22 bilirubin
23 bilirubin acid
24 bilirubin concentration
25 bilirubin load
26 bilirubin pool
27 bilirubin/albumin molar ratio
28 binding
29 blood cells
30 cell binding
31 cells
32 cellular content
33 cellular elements
34 cellular uptake
35 concentration
36 concentration of bilirubin
37 content
38 elements
39 equilibrium
40 factors
41 fraction
42 function
43 human serum albumin solution
44 increase
45 kernicterus
46 load
47 minutes
48 molar ratio
49 pH
50 partitioning
51 peroxidase assay
52 physiological pH
53 pool
54 ratio
55 red blood cells
56 risk
57 sensitivity
58 serum
59 serum albumin solution
60 serum unbound bilirubin concentrations
61 small fraction
62 solution
63 stoichiometry
64 total bilirubin concentration
65 toxicity
66 toxicity of bilirubin
67 unbound bilirubin concentration
68 uptake
69 vitro
70 schema:name 1037 FACTORS DETERMINING THE CELLULAR UPTAKE OF BILIRUBIN
71 schema:pagination 536-536
72 schema:productId N089af6a22626496dbc3c63e8476a1bbf
73 Nc61238fae50b440b899e90a974d8de6d
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018654951
75 https://doi.org/10.1203/00006450-197804001-01043
76 schema:sdDatePublished 2022-05-10T09:39
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher N629e34588ce84454a485299a00739a2e
79 schema:url https://doi.org/10.1203/00006450-197804001-01043
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N011db4b559e8486ea9ba55ac13a23ed9 schema:volumeNumber 12
84 rdf:type schema:PublicationVolume
85 N089af6a22626496dbc3c63e8476a1bbf schema:name doi
86 schema:value 10.1203/00006450-197804001-01043
87 rdf:type schema:PropertyValue
88 N24cc966e8f5a47feb02ca3af39c29402 rdf:first sg:person.060361100.77
89 rdf:rest rdf:nil
90 N629e34588ce84454a485299a00739a2e schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N8f7ab32cf42b48f4b22b21abab08471b rdf:first sg:person.070063300.96
93 rdf:rest N24cc966e8f5a47feb02ca3af39c29402
94 Nc61238fae50b440b899e90a974d8de6d schema:name dimensions_id
95 schema:value pub.1018654951
96 rdf:type schema:PropertyValue
97 Nf1f325f851c94fa18ddb0d74c83402a2 schema:issueNumber Suppl 4
98 rdf:type schema:PublicationIssue
99 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
100 schema:name Medical and Health Sciences
101 rdf:type schema:DefinedTerm
102 anzsrc-for:1114 schema:inDefinedTermSet anzsrc-for:
103 schema:name Paediatrics and Reproductive Medicine
104 rdf:type schema:DefinedTerm
105 anzsrc-for:1117 schema:inDefinedTermSet anzsrc-for:
106 schema:name Public Health and Health Services
107 rdf:type schema:DefinedTerm
108 sg:journal.1007352 schema:issn 0031-3998
109 1530-0447
110 schema:name Pediatric Research
111 schema:publisher Springer Nature
112 rdf:type schema:Periodical
113 sg:person.060361100.77 schema:affiliation grid-institutes:grid.27860.3b
114 schema:familyName Rasmussen
115 schema:givenName L Fraser
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.060361100.77
117 rdf:type schema:Person
118 sg:person.070063300.96 schema:affiliation grid-institutes:grid.27860.3b
119 schema:familyName Wennberg
120 schema:givenName Richard
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.070063300.96
122 rdf:type schema:Person
123 grid-institutes:grid.27860.3b schema:alternateName University of California, Davis - Sacramento Medical Center, Sacramento, California
124 schema:name University of California, Davis - Sacramento Medical Center, Sacramento, California
125 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...