Mechanisms Of Attachment By A Pathogenic Strain Of E. Coli (0111/B4) To Intestinal Mucosa In Pre- And Post Weanling Rats View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1977-04

AUTHORS

Moshe Hirschberger, David Mirelman, M Michael Thaler

ABSTRACT

Recent findings suggest that adhesiveness of certain strains of E. Coli to mammalian cells may be mediated by bacterial antigens with affinity for mannose residues of surface glycoproteins. We investigated the possibility that attachnent of E. Coli to intestinal muoosal cells may be inhibited or reversed by mannose polymer. Mucosal scrapings from duodenum (D), jejunum (J), ileum (IL), and colon (C) of 7 day-old and 3 week-old rats were preincubated with a 3H-thymidine-labeled strain of E. Coli associated with severe infantile diarrhea (0111/B4). Mannose polymer (mannan) or sucrose were added, incubation terminated after 30 min at 37°C, tissues washed free of unattached bacteria, and bound radioactivity counted. Mucosa from 7 day-old rats bound 8% of added bacteria in D, 30% in J, 14% in IL and 25% in C. Binding to mucosa from 3 week-old rats was comparatively minor (0.6% in D, 0.5% in J, 1.2% in IL, 2.6% in C). Incubation with mannan reduced binding in all segments of 7 day-old intestine by 93 to 98%, whereas inhibition in 3 week-old specimens averaged 20%. These results indicate that E. Coli 0111/B4 attach to pre-weanling intestine by a specific mechanism which may be reversed by mannose derivatives. Binding to post-weanling mucosa is much less effective, and is either partly mediated by a different mechanism, or is relatively non-specific. More... »

PAGES

500-500

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-197704000-00784

DOI

http://dx.doi.org/10.1203/00006450-197704000-00784

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034870386


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