147: Regulation Of An Enzyme Activity During Myelination By Membrane Lipids View Full Text


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Article Info

DATE

1976-10

AUTHORS

H P Siegrist, A J Steck, Th Burkart, N Herschkowitz

ABSTRACT

Sulphatide, an acidic sphingolipid, is an important component of myelin. Its rate of synthesis changes significantly during development. In mouse brain, the synthesis starts at the 8th postnatal day, reaches a maximum at day 16 and declines until day 25, when a base rate of synthesis is achieved. To investigate the mechanism of the regulation of this developmental pattern, microsomes of mouse brain were delipidated and a lipid requirement of a key enzyme of the sulphatide-synthesis, the Cerebroside-Sulphotransferase (CST), could be shown. We could then demonstrate, that the residual activity of delipidated microsomes from brains of 8 to 25 day old mice remained at the same level, this in contrast to whole microsomes, where the CST-actvity pattern corresponded to the one found in vivo. Reconstitution experiments with delipidated enzyme preparations and the corresponding lipids of different ages showed a final CST-activity pattern depending on the age of the lipid and not on the age of the enzyme source. The results suggest, that the age dependent CST-activities are at least partly regulated by the lipids associated with the enzyme protein in the microsomal membrane. More... »

PAGES

894

Journal

TITLE

Pediatric Research

ISSUE

10

VOLUME

10

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-197610000-00138

DOI

http://dx.doi.org/10.1203/00006450-197610000-00138

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023403846


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