Uptake Of Serum Bilirubin-14C (Sb) By Erythrocytes Crbc) From Newborns And Adults View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1974-04

AUTHORS

Abraham Gotlieb, Michael N Applebaum, M Michael Thaler, Louis K Diamond

ABSTRACT

Tissue damage in jaundiced newborns is due to excessive intracellular deposits of SB. Radio-labeled SB was used to measure uptake of SB from serum by a readily accessible tissue (RBC). RBC and plasma from 0.5 ml heparinized blood were separated, and RBC resuspended in the original plasma diluted to different bilirubin/albumin ratios (B/A) with buffer and SB. After incubation, RBC's were washed and RBC cpm measured to determine RBC-B. Uptake of SB by RBC was complete in 15 minutes at all B/A (0.4-4.0). At B/A < 1, RBC-B was < 10% SB. At B/A 1.0-1.6, RBC-B increased 5-fold. RBC were saturated at B/A ≥ 2.0. Individual uptake patterns characterized each infant. Membrane-bound SB was ~ 20% of RBC-B. Newborn and adult RBC had similar affinities for SB. These results indicate that some SB enters RBC at relatively low B/A, and increases rapidly in a narrow B/A range peculiar to each infant. This direct, rapid and sensitive microassay of RBC-B may be useful in predicting the possibility of damage to newborn tissues from excessive SB.(Supported by The Cerebral Palsy Foundation) More... »

PAGES

401-401

Identifiers

URI

http://scigraph.springernature.com/pub.10.1203/00006450-197404000-00366

DOI

http://dx.doi.org/10.1203/00006450-197404000-00366

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017882005


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1114", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Paediatrics and Reproductive Medicine", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, University of California, San Francisco", 
          "id": "http://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Department of Pediatrics, University of California, San Francisco"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gotlieb", 
        "givenName": "Abraham", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, University of California, San Francisco", 
          "id": "http://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Department of Pediatrics, University of California, San Francisco"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Applebaum", 
        "givenName": "Michael N", 
        "id": "sg:person.0100225063.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0100225063.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, University of California, San Francisco", 
          "id": "http://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Department of Pediatrics, University of California, San Francisco"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thaler", 
        "givenName": "M Michael", 
        "id": "sg:person.072023511.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.072023511.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, University of California, San Francisco", 
          "id": "http://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Department of Pediatrics, University of California, San Francisco"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Diamond", 
        "givenName": "Louis K", 
        "id": "sg:person.064531736.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.064531736.40"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1974-04", 
    "datePublishedReg": "1974-04-01", 
    "description": "Tissue damage in jaundiced newborns is due to excessive intracellular deposits of SB. Radio-labeled SB was used to measure uptake of SB from serum by a readily accessible tissue (RBC). RBC and plasma from 0.5 ml heparinized blood were separated, and RBC resuspended in the original plasma diluted to different bilirubin/albumin ratios (B/A) with buffer and SB. After incubation, RBC's were washed and RBC cpm measured to determine RBC-B. Uptake of SB by RBC was complete in 15 minutes at all B/A (0.4-4.0). At B/A < 1, RBC-B was < 10% SB. At B/A 1.0-1.6, RBC-B increased 5-fold. RBC were saturated at B/A \u2265 2.0. Individual uptake patterns characterized each infant. Membrane-bound SB was ~ 20% of RBC-B. Newborn and adult RBC had similar affinities for SB. These results indicate that some SB enters RBC at relatively low B/A, and increases rapidly in a narrow B/A range peculiar to each infant. This direct, rapid and sensitive microassay of RBC-B may be useful in predicting the possibility of damage to newborn tissues from excessive SB.(Supported by The Cerebral Palsy Foundation)", 
    "genre": "article", 
    "id": "sg:pub.10.1203/00006450-197404000-00366", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1007352", 
        "issn": [
          "0031-3998", 
          "1530-0447"
        ], 
        "name": "Pediatric Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "bilirubin/albumin ratio", 
      "albumin ratio", 
      "jaundiced newborns", 
      "tissue damage", 
      "heparinized blood", 
      "newborn tissues", 
      "accessible tissue", 
      "intracellular deposits", 
      "newborns", 
      "infants", 
      "RBCs", 
      "adult RBCs", 
      "uptake patterns", 
      "tissue", 
      "sensitive microassay", 
      "uptake", 
      "similar affinity", 
      "original plasma", 
      "CRBC", 
      "blood", 
      "damage", 
      "serum", 
      "adults", 
      "possibility of damage", 
      "minutes", 
      "microassay", 
      "incubation", 
      "plasma", 
      "increase", 
      "affinity", 
      "patterns", 
      "membrane", 
      "ratio", 
      "results", 
      "possibility", 
      "buffer", 
      "range", 
      "deposits", 
      "excessive intracellular deposits", 
      "SB", 
      "Radio-labeled SB", 
      "uptake of SB", 
      "different bilirubin/albumin ratios", 
      "Individual uptake patterns", 
      "excessive SB", 
      "BILIRUBIN-14C", 
      "ERYTHROCYTES CRBC"
    ], 
    "name": "UPTAKE OF SERUM BILIRUBIN-14C (SB) BY ERYTHROCYTES CRBC) FROM NEWBORNS AND ADULTS", 
    "pagination": "401-401", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017882005"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1203/00006450-197404000-00366"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1203/00006450-197404000-00366", 
      "https://app.dimensions.ai/details/publication/pub.1017882005"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_129.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1203/00006450-197404000-00366"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1203/00006450-197404000-00366'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1203/00006450-197404000-00366'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1203/00006450-197404000-00366'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1203/00006450-197404000-00366'


 

This table displays all metadata directly associated to this object as RDF triples.

125 TRIPLES      21 PREDICATES      73 URIs      65 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1203/00006450-197404000-00366 schema:about anzsrc-for:11
2 anzsrc-for:1114
3 schema:author N2763c42967fd4e02a5dd42219c16fcae
4 schema:datePublished 1974-04
5 schema:datePublishedReg 1974-04-01
6 schema:description Tissue damage in jaundiced newborns is due to excessive intracellular deposits of SB. Radio-labeled SB was used to measure uptake of SB from serum by a readily accessible tissue (RBC). RBC and plasma from 0.5 ml heparinized blood were separated, and RBC resuspended in the original plasma diluted to different bilirubin/albumin ratios (B/A) with buffer and SB. After incubation, RBC's were washed and RBC cpm measured to determine RBC-B. Uptake of SB by RBC was complete in 15 minutes at all B/A (0.4-4.0). At B/A < 1, RBC-B was < 10% SB. At B/A 1.0-1.6, RBC-B increased 5-fold. RBC were saturated at B/A ≥ 2.0. Individual uptake patterns characterized each infant. Membrane-bound SB was ~ 20% of RBC-B. Newborn and adult RBC had similar affinities for SB. These results indicate that some SB enters RBC at relatively low B/A, and increases rapidly in a narrow B/A range peculiar to each infant. This direct, rapid and sensitive microassay of RBC-B may be useful in predicting the possibility of damage to newborn tissues from excessive SB.(Supported by The Cerebral Palsy Foundation)
7 schema:genre article
8 schema:inLanguage en
9 schema:isAccessibleForFree true
10 schema:isPartOf N1dd3616605964cd58bed19591fd9a0ac
11 N82dfc5eefd7b4c9aa739ffbe4ae66c2f
12 sg:journal.1007352
13 schema:keywords BILIRUBIN-14C
14 CRBC
15 ERYTHROCYTES CRBC
16 Individual uptake patterns
17 RBCs
18 Radio-labeled SB
19 SB
20 accessible tissue
21 adult RBCs
22 adults
23 affinity
24 albumin ratio
25 bilirubin/albumin ratio
26 blood
27 buffer
28 damage
29 deposits
30 different bilirubin/albumin ratios
31 excessive SB
32 excessive intracellular deposits
33 heparinized blood
34 increase
35 incubation
36 infants
37 intracellular deposits
38 jaundiced newborns
39 membrane
40 microassay
41 minutes
42 newborn tissues
43 newborns
44 original plasma
45 patterns
46 plasma
47 possibility
48 possibility of damage
49 range
50 ratio
51 results
52 sensitive microassay
53 serum
54 similar affinity
55 tissue
56 tissue damage
57 uptake
58 uptake of SB
59 uptake patterns
60 schema:name UPTAKE OF SERUM BILIRUBIN-14C (SB) BY ERYTHROCYTES CRBC) FROM NEWBORNS AND ADULTS
61 schema:pagination 401-401
62 schema:productId N1beedc8a48464e3da39a31db4d127e13
63 Na2e7ca9203a74533b31db5b5a62c9251
64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017882005
65 https://doi.org/10.1203/00006450-197404000-00366
66 schema:sdDatePublished 2022-01-01T18:00
67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
68 schema:sdPublisher Nfdee56f7e2d146adb84f56024f030f1a
69 schema:url https://doi.org/10.1203/00006450-197404000-00366
70 sgo:license sg:explorer/license/
71 sgo:sdDataset articles
72 rdf:type schema:ScholarlyArticle
73 N01b30236521043339f9642f4d4d9b772 rdf:first sg:person.064531736.40
74 rdf:rest rdf:nil
75 N1beedc8a48464e3da39a31db4d127e13 schema:name doi
76 schema:value 10.1203/00006450-197404000-00366
77 rdf:type schema:PropertyValue
78 N1dd3616605964cd58bed19591fd9a0ac schema:issueNumber 4
79 rdf:type schema:PublicationIssue
80 N2763c42967fd4e02a5dd42219c16fcae rdf:first N4d407a9549134302b73c45b0faa9dced
81 rdf:rest Nc0f5998e2c904f3c960edc1e6a0628de
82 N4d407a9549134302b73c45b0faa9dced schema:affiliation grid-institutes:grid.266102.1
83 schema:familyName Gotlieb
84 schema:givenName Abraham
85 rdf:type schema:Person
86 N6bbd494d17e14e1d9fd8fb23c03a1b4d rdf:first sg:person.072023511.91
87 rdf:rest N01b30236521043339f9642f4d4d9b772
88 N82dfc5eefd7b4c9aa739ffbe4ae66c2f schema:volumeNumber 8
89 rdf:type schema:PublicationVolume
90 Na2e7ca9203a74533b31db5b5a62c9251 schema:name dimensions_id
91 schema:value pub.1017882005
92 rdf:type schema:PropertyValue
93 Nc0f5998e2c904f3c960edc1e6a0628de rdf:first sg:person.0100225063.92
94 rdf:rest N6bbd494d17e14e1d9fd8fb23c03a1b4d
95 Nfdee56f7e2d146adb84f56024f030f1a schema:name Springer Nature - SN SciGraph project
96 rdf:type schema:Organization
97 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
98 schema:name Medical and Health Sciences
99 rdf:type schema:DefinedTerm
100 anzsrc-for:1114 schema:inDefinedTermSet anzsrc-for:
101 schema:name Paediatrics and Reproductive Medicine
102 rdf:type schema:DefinedTerm
103 sg:journal.1007352 schema:issn 0031-3998
104 1530-0447
105 schema:name Pediatric Research
106 schema:publisher Springer Nature
107 rdf:type schema:Periodical
108 sg:person.0100225063.92 schema:affiliation grid-institutes:grid.266102.1
109 schema:familyName Applebaum
110 schema:givenName Michael N
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0100225063.92
112 rdf:type schema:Person
113 sg:person.064531736.40 schema:affiliation grid-institutes:grid.266102.1
114 schema:familyName Diamond
115 schema:givenName Louis K
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.064531736.40
117 rdf:type schema:Person
118 sg:person.072023511.91 schema:affiliation grid-institutes:grid.266102.1
119 schema:familyName Thaler
120 schema:givenName M Michael
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.072023511.91
122 rdf:type schema:Person
123 grid-institutes:grid.266102.1 schema:alternateName Department of Pediatrics, University of California, San Francisco
124 schema:name Department of Pediatrics, University of California, San Francisco
125 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...