Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos View Full Text


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Article Info

DATE

2021-05-11

AUTHORS

Masoumeh Nomani, Masoud Tohidfar

ABSTRACT

BackgroundTrachyspermum ammi is one of the key medicinal plant species with many beneficial properties. Thymol is the most important substance in the essential oil of this plant. Thymol is a natural monoterpene phenol with high anti-microbial, anti-bacterial, and anti-oxidant properties. Thymol in the latest research has a significant impact on slowing the progression of cancer cells in human. In this research, embryos were employed as convenient explants for the fast and effectual regeneration and transformation of T. ammi. To regenerate this plant, Murashige and Skoog (MS) and Gamborg's B5 (B5) media were supplemented with diverse concentrations of plant growth regulators, such as 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (kin). Transgenic Trachyspermum ammi plants were also obtained using Agrobacterium-mediated transformation and zygotic embryos explants. Moreover, two Agrobacterium tumefaciens strains (EHA101 and LBA4404) harboring pBI121-TPS2 were utilized for genetic transformation to Trachyspermum ammi.ResultsAccording to the obtained results, the highest plant-regeneration frequency was obtained with B5 medium supplemented with 0.5 mg/l BA and 1 mg/l NAA. The integrated gene was also approved using the PCR reaction and the Southern blot method. Results also showed that the EHA101 strain outperformed another strain in inoculation time (30 s) and co-cultivation period (1 day) (transformation efficiency 19.29%). Furthermore, HPLC method demonstrated that the transformed plants contained a higher thymol level than non-transformed plants.ConclusionsIn this research, a fast protocol was introduced for the regeneration and transformation of Trachyspermum ammi, using zygotic embryo explants in 25–35 days. Our findings confirmed the increase in the thymol in the aerial part of Trachyspermum ammi. We further presented an efficacious technique for enhancing thymol content in Trachyspermum ammi using Agrobacterium-mediated plant transformation system that can be beneficial in genetic transformation and other plant biotechnology techniques. More... »

PAGES

68

References to SciGraph publications

  • 2014-04-30. Plant regeneration through direct somatic embryogenesis from immature zygotic embryos of the medicinal plant, Parispolyphylla Sm. in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2017-08-14. Genetic engineering approach using early Vinca alkaloid biosynthesis genes led to increased tryptamine and terpenoid indole alkaloids biosynthesis in differentiating cultures of Catharanthus roseus in PROTOPLASMA
  • 2006-02-03. Genetic and biochemical analysis of Hypericum perforatum L. plants regenerated after cryopreservation in PLANT CELL REPORTS
  • 2012-11-18. Metabolic engineering of morphinan alkaloids by over-expression of codeinone reductase in transgenic hairy roots of Papaver bracteatum, the Iranian poppy in BIOTECHNOLOGY LETTERS
  • 2013-06-30. An Efficient Method for Agrobacterium-Mediated Genetic Transformation and Plant Regeneration in Cumin (Cuminum cyminum L.) in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2012-10-26. Stimulation of in vitro organogenesis from epicotyl explants and successive micropropagation round in Cassia angustifolia Vahl.: an important source of sennosides in AGROFORESTRY SYSTEMS
  • 2017-07-06. Characterization of the formation of somatic embryos from mature zygotic embryos of Passiflora ligularis Juss. in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2010-10-17. Embryo rescue and plant regeneration in banana (Musa spp.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2020-02-26. Establishment and optimization of a hemp (Cannabis sativa L.) agroinfiltration system for gene expression and silencing studies in SCIENTIFIC REPORTS
  • 2006-12. In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd in BIOLOGIA PLANTARUM
  • 2008-05-24. Molecular approaches for improvement of medicinal and aromatic plants in PLANT BIOTECHNOLOGY REPORTS
  • 2011-08-09. In vitro plant regeneration and de novo differentiation of secretory trichomes in Passiflora foetida L. (Passifloraceae) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2009-12-11. Agrobacterium tumefaciens-mediated transformation of Withania somnifera (L.) Dunal: an important medicinal plant in PLANT CELL REPORTS
  • 2007-04-18. Direct somatic embryogenesis from cotyledon and cotyledonary node explants in bishop’s weed Trachyspermum ammi (L.) sprague in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2013-04-28. Genomic characterization, molecular cloning and expression analysis of two terpene synthases from Thymus caespititius (Lamiaceae) in PLANTA
  • 2014-12. Strain specific Agrobacterium-mediated genetic transformation of Bacopa monnieri in JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
  • 2001-01. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment in MOLECULAR BREEDING
  • 2010-02-05. High frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures in PLANT BIOTECHNOLOGY REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s43141-021-00173-8

    DOI

    http://dx.doi.org/10.1186/s43141-021-00173-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1137930500

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33974146


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