Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-09-05

AUTHORS

Alireza Kashani, Asker Daniel Brejnrod, Chunyu Jin, Timo Kern, Andreas Nygaard Madsen, Louise Aas Holm, Georg K. Gerber, Jens-Christian Holm, Torben Hansen, Birgitte Holst, Manimozhiyan Arumugam

ABSTRACT

BackgroundGrowing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes. Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity. Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy. However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.ResultsWe performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum. To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice. Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage. Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass. Acclimatization of the microbiota to our facility took up to 5 weeks. Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance. Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different. We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila. Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice. Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).ConclusionsLeptin deficiency caused major changes in the mouse gut microbiota composition. Several microbial taxa were associated with body composition. Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals. More... »

PAGES

11

References to SciGraph publications

  • 2012-09-26. A metagenome-wide association study of gut microbiota in type 2 diabetes in NATURE
  • 2016-05-23. DADA2: High-resolution sample inference from Illumina amplicon data in NATURE METHODS
  • 2006-12. An obesity-associated gut microbiome with increased capacity for energy harvest in NATURE
  • 2015-11-13. Akkermansia muciniphila inversely correlates with the onset of inflammation, altered adipose tissue metabolism and metabolic disorders during obesity in mice in SCIENTIFIC REPORTS
  • 2016-07-13. Human gut microbes impact host serum metabolome and insulin sensitivity in NATURE
  • 2010-04-11. QIIME allows analysis of high-throughput community sequencing data in NATURE METHODS
  • 2015-09-28. A catalog of the mouse gut metagenome in NATURE BIOTECHNOLOGY
  • 2013-08-28. Richness of human gut microbiome correlates with metabolic markers in NATURE
  • 2015-09-16. In silico identification of bacteriocin gene clusters in the gastrointestinal tract, based on the Human Microbiome Project’s reference genome database in BMC MICROBIOLOGY
  • 2014-04-03. Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2006-07-28. The role of leptin in innate and adaptive immune responses in ARTHRITIS RESEARCH & THERAPY
  • 2008-11-30. A core gut microbiome in obese and lean twins in NATURE
  • 2016-11-18. Environmental spread of microbes impacts the development of metabolic phenotypes in mice transplanted with microbial communities from humans in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2016-06-15. Roux-en-Y gastric bypass surgery of morbidly obese patients induces swift and persistent changes of the individual gut microbiota in GENOME MEDICINE
  • 2013-12-11. Diet rapidly and reproducibly alters the human gut microbiome in NATURE
  • 2010-09-09. UniFrac: an effective distance metric for microbial community comparison in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2013-05-29. Gut metagenome in European women with normal, impaired and diabetic glucose control in NATURE
  • 2017-03-03. Normalization and microbial differential abundance strategies depend upon data characteristics in MICROBIOME
  • 2017-07-21. Exact sequence variants should replace operational taxonomic units in marker-gene data analysis in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2019-02-19. Sequence and cultivation study of Muribaculaceae reveals novel species, host preference, and functional potential of this yet undescribed family in MICROBIOME
  • 2014-12-05. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 in GENOME BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s42523-019-0007-1

    DOI

    http://dx.doi.org/10.1186/s42523-019-0007-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1120879733

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33499919


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Clinical Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Qbiom Microbiome Consultancy Service, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "Danish Diabetes Academy, Odense, Denmark", 
                "Qbiom Microbiome Consultancy Service, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kashani", 
            "givenName": "Alireza", 
            "id": "sg:person.01041203677.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041203677.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brejnrod", 
            "givenName": "Asker Daniel", 
            "id": "sg:person.0702056330.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702056330.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jin", 
            "givenName": "Chunyu", 
            "id": "sg:person.01262662636.78", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262662636.78"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kern", 
            "givenName": "Timo", 
            "id": "sg:person.01157753220.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157753220.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Madsen", 
            "givenName": "Andreas Nygaard", 
            "id": "sg:person.01006004720.78", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006004720.78"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Children\u2019s Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holb\u00e6k, 4300, Holb\u00e6k, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.4973.9", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "The Children\u2019s Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holb\u00e6k, 4300, Holb\u00e6k, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Holm", 
            "givenName": "Louise Aas", 
            "id": "sg:person.012204604131.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012204604131.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Brigham & Women\u2019s Hospital, Harvard Medical School, Boston, MA, USA", 
              "id": "http://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Pathology, Brigham & Women\u2019s Hospital, Harvard Medical School, Boston, MA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gerber", 
            "givenName": "Georg K.", 
            "id": "sg:person.01067252435.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067252435.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The Children\u2019s Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holb\u00e6k, 4300, Holb\u00e6k, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.4973.9", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "The Children\u2019s Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holb\u00e6k, 4300, Holb\u00e6k, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Holm", 
            "givenName": "Jens-Christian", 
            "id": "sg:person.01106620303.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106620303.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hansen", 
            "givenName": "Torben", 
            "id": "sg:person.016350427407.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016350427407.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Holst", 
            "givenName": "Birgitte", 
            "id": "sg:person.0721620336.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721620336.76"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.10825.3e", 
              "name": [
                "Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark", 
                "Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Arumugam", 
            "givenName": "Manimozhiyan", 
            "id": "sg:person.01235472333.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235472333.35"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature07540", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030170002", 
              "https://doi.org/10.1038/nature07540"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.3353", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049321285", 
              "https://doi.org/10.1038/nbt.3353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13059-014-0550-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015222646", 
              "https://doi.org/10.1186/s13059-014-0550-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05414", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023893418", 
              "https://doi.org/10.1038/nature05414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2014.45", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051037244", 
              "https://doi.org/10.1038/ismej.2014.45"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep16643", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023137628", 
              "https://doi.org/10.1038/srep16643"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12866-015-0515-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006218857", 
              "https://doi.org/10.1186/s12866-015-0515-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature18646", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017924056", 
              "https://doi.org/10.1038/nature18646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12198", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002791386", 
              "https://doi.org/10.1038/nature12198"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature11450", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004546178", 
              "https://doi.org/10.1038/nature11450"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.3869", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016631324", 
              "https://doi.org/10.1038/nmeth.3869"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13073-016-0312-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015450395", 
              "https://doi.org/10.1186/s13073-016-0312-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.f.303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009032055", 
              "https://doi.org/10.1038/nmeth.f.303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s40168-017-0237-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084252802", 
              "https://doi.org/10.1186/s40168-017-0237-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2016.151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017331773", 
              "https://doi.org/10.1038/ismej.2016.151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2010.133", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042916034", 
              "https://doi.org/10.1038/ismej.2010.133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/ar2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006944697", 
              "https://doi.org/10.1186/ar2004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2017.119", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090852589", 
              "https://doi.org/10.1038/ismej.2017.119"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s40168-019-0637-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1112223842", 
              "https://doi.org/10.1186/s40168-019-0637-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12820", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013830289", 
              "https://doi.org/10.1038/nature12820"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12506", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003823032", 
              "https://doi.org/10.1038/nature12506"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-09-05", 
        "datePublishedReg": "2019-09-05", 
        "description": "BackgroundGrowing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes. Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity. Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy. However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.ResultsWe performed a 10-week long study in ob/ob (n\u00a0=\u200912) and wild type control (n\u2009=\u200912) mice fed ad libitum. To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n\u00a0=\u200911) based on the food consumption of control mice. Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage. Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass. Acclimatization of the microbiota to our facility took up to 5\u2009weeks. Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance. Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different. We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila. Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice. Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).ConclusionsLeptin deficiency caused major changes in the mouse gut microbiota composition. Several microbial taxa were associated with body composition. Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/s42523-019-0007-1", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.9011955", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.9011173", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1336340", 
            "issn": [
              "2524-4671"
            ], 
            "name": "Animal Microbiome", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "1"
          }
        ], 
        "keywords": [
          "ob/ob mice", 
          "mice fed ad libitum", 
          "leptin deficiency", 
          "ob mice", 
          "body fat percentage", 
          "glucose metabolism", 
          "gut microbiota", 
          "fed ad libitum", 
          "pair-fed ob/ob mice", 
          "control mice fed ad libitum", 
          "higher body fat percentage", 
          "role of hyperphagia", 
          "fat percentage", 
          "reduced calorie intake", 
          "pair-fed mice", 
          "impaired glucose metabolism", 
          "altered gut microbiome", 
          "ad libitum", 
          "type 2 diabetes", 
          "ob/ob", 
          "model of obesity", 
          "gut microbiota composition", 
          "lean body mass", 
          "gut microbial composition", 
          "mouse gut microbiota", 
          "control mice", 
          "obese individuals", 
          "treatment options", 
          "Bacteroidetes ratio", 
          "metabolic impairment", 
          "Akkermansia muciniphila", 
          "body fat", 
          "calorie intake", 
          "obesity", 
          "body weight", 
          "body composition", 
          "microbiota composition", 
          "calorie restriction", 
          "related disorders", 
          "BackgroundGrowing evidence", 
          "gut microbiome", 
          "mice", 
          "additional group", 
          "wild type microbiota", 
          "food consumption", 
          "microbiota", 
          "hyperphagia", 
          "metabolic functions", 
          "metabolism", 
          "deficiency", 
          "body mass", 
          "higher proportion", 
          "long study", 
          "specific changes", 
          "libitum", 
          "enrichment of members", 
          "microbial composition", 
          "differential development", 
          "diabetes", 
          "muciniphila", 
          "percentage", 
          "Alistipes", 
          "intake", 
          "impairment", 
          "weeks", 
          "ResultsWe", 
          "glycan degradation", 
          "disorders", 
          "ABC transporters", 
          "Prevotella", 
          "Oscillibacter", 
          "fat", 
          "study", 
          "microbial metabolic functions", 
          "association", 
          "role", 
          "Lactobacillus", 
          "microbiome", 
          "major implications", 
          "group", 
          "options", 
          "changes", 
          "transporters", 
          "OB", 
          "proportion", 
          "individuals", 
          "evidence", 
          "depletion", 
          "major changes", 
          "weight", 
          "effect", 
          "microbial taxa", 
          "restriction", 
          "mass", 
          "function", 
          "Firmicutes", 
          "acclimatization", 
          "microbial diversity", 
          "development", 
          "members", 
          "facilities", 
          "ratio", 
          "consumption", 
          "end", 
          "energy harvest", 
          "implications", 
          "variance", 
          "capacity", 
          "link", 
          "composition", 
          "model", 
          "enrichment", 
          "cost", 
          "system", 
          "degradation", 
          "phosphotransferase system", 
          "harvest", 
          "diversity", 
          "overall community composition", 
          "taxa associations", 
          "taxa", 
          "community composition", 
          "energy"
        ], 
        "name": "Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice", 
        "pagination": "11", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1120879733"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s42523-019-0007-1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "33499919"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s42523-019-0007-1", 
          "https://app.dimensions.ai/details/publication/pub.1120879733"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T21:04", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_820.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/s42523-019-0007-1"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s42523-019-0007-1'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s42523-019-0007-1'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s42523-019-0007-1'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s42523-019-0007-1'


     

    This table displays all metadata directly associated to this object as RDF triples.

    359 TRIPLES      21 PREDICATES      169 URIs      140 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s42523-019-0007-1 schema:about anzsrc-for:11
    2 anzsrc-for:1103
    3 schema:author N543d29e510874a6d90da14d444965614
    4 schema:citation sg:pub.10.1038/ismej.2010.133
    5 sg:pub.10.1038/ismej.2014.45
    6 sg:pub.10.1038/ismej.2016.151
    7 sg:pub.10.1038/ismej.2017.119
    8 sg:pub.10.1038/nature05414
    9 sg:pub.10.1038/nature07540
    10 sg:pub.10.1038/nature11450
    11 sg:pub.10.1038/nature12198
    12 sg:pub.10.1038/nature12506
    13 sg:pub.10.1038/nature12820
    14 sg:pub.10.1038/nature18646
    15 sg:pub.10.1038/nbt.3353
    16 sg:pub.10.1038/nmeth.3869
    17 sg:pub.10.1038/nmeth.f.303
    18 sg:pub.10.1038/srep16643
    19 sg:pub.10.1186/ar2004
    20 sg:pub.10.1186/s12866-015-0515-4
    21 sg:pub.10.1186/s13059-014-0550-8
    22 sg:pub.10.1186/s13073-016-0312-1
    23 sg:pub.10.1186/s40168-017-0237-y
    24 sg:pub.10.1186/s40168-019-0637-2
    25 schema:datePublished 2019-09-05
    26 schema:datePublishedReg 2019-09-05
    27 schema:description BackgroundGrowing evidence supports the role of gut microbiota in obesity and its related disorders including type 2 diabetes. Ob/ob mice, which are hyperphagic due to leptin deficiency, are commonly used models of obesity and were instrumental in suggesting links between gut microbiota and obesity. Specific changes in their gut microbiota such as decreased microbial diversity and increased Firmicutes to Bacteroidetes ratio have been suggested to contribute to obesity via increased microbiota capacity to harvest energy. However, the differential development of ob/ob mouse gut microbiota compared to wild type microbiota and the role of hyperphagia in their metabolic impairment have not been investigated thoroughly.ResultsWe performed a 10-week long study in ob/ob (n = 12) and wild type control (n = 12) mice fed ad libitum. To differentiate effects of leptin deficiency from hyperphagia, we pair-fed an additional group of ob/ob mice (n = 11) based on the food consumption of control mice. Compared to control mice, ob/ob mice fed ad libitum exhibited compromised glucose metabolism and increased body fat percentage. Pair-fed ob/ob mice exhibited even more compromised glucose metabolism and maintained strikingly similar high body fat percentage at the cost of lean body mass. Acclimatization of the microbiota to our facility took up to 5 weeks. Leptin deficiency impacted gut microbial composition, explaining 18.3% of the variance. Pair-feeding also altered several taxa, although the overall community composition at the end of the study was not significantly different. We found 24 microbial taxa associations with leptin deficiency, notably enrichment of members of Lactobacillus and depletion of Akkermansia muciniphila. Microbial metabolic functions related to energy harvest, including glycan degradation, phosphotransferase systems and ABC transporters, were enriched in the ob/ob mice. Taxa previously reported as relevant for obesity were associated with body weight, including Oscillibacter and Alistipes (both negatively correlated) and Prevotella (positively correlated).ConclusionsLeptin deficiency caused major changes in the mouse gut microbiota composition. Several microbial taxa were associated with body composition. Pair-fed mice maintained a pre-set high proportion of body fat despite reduced calorie intake, and exhibited more compromised glucose metabolism, with major implications for treatment options for genetically obese individuals.
    28 schema:genre article
    29 schema:isAccessibleForFree true
    30 schema:isPartOf N3282f13f9ff64737a904ba1c6732a06a
    31 N7348e7ae06b24be98dd7c8226f922b49
    32 sg:journal.1336340
    33 schema:keywords ABC transporters
    34 Akkermansia muciniphila
    35 Alistipes
    36 BackgroundGrowing evidence
    37 Bacteroidetes ratio
    38 Firmicutes
    39 Lactobacillus
    40 OB
    41 Oscillibacter
    42 Prevotella
    43 ResultsWe
    44 acclimatization
    45 ad libitum
    46 additional group
    47 altered gut microbiome
    48 association
    49 body composition
    50 body fat
    51 body fat percentage
    52 body mass
    53 body weight
    54 calorie intake
    55 calorie restriction
    56 capacity
    57 changes
    58 community composition
    59 composition
    60 consumption
    61 control mice
    62 control mice fed ad libitum
    63 cost
    64 deficiency
    65 degradation
    66 depletion
    67 development
    68 diabetes
    69 differential development
    70 disorders
    71 diversity
    72 effect
    73 end
    74 energy
    75 energy harvest
    76 enrichment
    77 enrichment of members
    78 evidence
    79 facilities
    80 fat
    81 fat percentage
    82 fed ad libitum
    83 food consumption
    84 function
    85 glucose metabolism
    86 glycan degradation
    87 group
    88 gut microbial composition
    89 gut microbiome
    90 gut microbiota
    91 gut microbiota composition
    92 harvest
    93 higher body fat percentage
    94 higher proportion
    95 hyperphagia
    96 impaired glucose metabolism
    97 impairment
    98 implications
    99 individuals
    100 intake
    101 lean body mass
    102 leptin deficiency
    103 libitum
    104 link
    105 long study
    106 major changes
    107 major implications
    108 mass
    109 members
    110 metabolic functions
    111 metabolic impairment
    112 metabolism
    113 mice
    114 mice fed ad libitum
    115 microbial composition
    116 microbial diversity
    117 microbial metabolic functions
    118 microbial taxa
    119 microbiome
    120 microbiota
    121 microbiota composition
    122 model
    123 model of obesity
    124 mouse gut microbiota
    125 muciniphila
    126 ob mice
    127 ob/ob
    128 ob/ob mice
    129 obese individuals
    130 obesity
    131 options
    132 overall community composition
    133 pair-fed mice
    134 pair-fed ob/ob mice
    135 percentage
    136 phosphotransferase system
    137 proportion
    138 ratio
    139 reduced calorie intake
    140 related disorders
    141 restriction
    142 role
    143 role of hyperphagia
    144 specific changes
    145 study
    146 system
    147 taxa
    148 taxa associations
    149 transporters
    150 treatment options
    151 type 2 diabetes
    152 variance
    153 weeks
    154 weight
    155 wild type microbiota
    156 schema:name Impaired glucose metabolism and altered gut microbiome despite calorie restriction of ob/ob mice
    157 schema:pagination 11
    158 schema:productId N232aed8a46984e9eb51edef505fd3dab
    159 N3328a981759a4d9c804b9a295d163978
    160 N684bad835fa348d3ab50ea9474a99f14
    161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1120879733
    162 https://doi.org/10.1186/s42523-019-0007-1
    163 schema:sdDatePublished 2022-11-24T21:04
    164 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    165 schema:sdPublisher N33eb4da541764056a41aec3bfcccb4ab
    166 schema:url https://doi.org/10.1186/s42523-019-0007-1
    167 sgo:license sg:explorer/license/
    168 sgo:sdDataset articles
    169 rdf:type schema:ScholarlyArticle
    170 N232aed8a46984e9eb51edef505fd3dab schema:name dimensions_id
    171 schema:value pub.1120879733
    172 rdf:type schema:PropertyValue
    173 N24931cf1a20c4103a8505d9d5b763369 rdf:first sg:person.016350427407.47
    174 rdf:rest N42e6575e594e441ca7330ea7dde359f7
    175 N3282f13f9ff64737a904ba1c6732a06a schema:issueNumber 1
    176 rdf:type schema:PublicationIssue
    177 N3328a981759a4d9c804b9a295d163978 schema:name pubmed_id
    178 schema:value 33499919
    179 rdf:type schema:PropertyValue
    180 N33eb4da541764056a41aec3bfcccb4ab schema:name Springer Nature - SN SciGraph project
    181 rdf:type schema:Organization
    182 N36ae3379beb5466694e3d1282b5f5f4f rdf:first sg:person.01262662636.78
    183 rdf:rest Nb32f5f6c5333431bb375acb5b0cc5d14
    184 N42e6575e594e441ca7330ea7dde359f7 rdf:first sg:person.0721620336.76
    185 rdf:rest Nf04e48a2940d4569abce8f79c38a1c1f
    186 N543d29e510874a6d90da14d444965614 rdf:first sg:person.01041203677.36
    187 rdf:rest Nd5b0073c3c3a4ddf868a5c65019b519f
    188 N684bad835fa348d3ab50ea9474a99f14 schema:name doi
    189 schema:value 10.1186/s42523-019-0007-1
    190 rdf:type schema:PropertyValue
    191 N7348e7ae06b24be98dd7c8226f922b49 schema:volumeNumber 1
    192 rdf:type schema:PublicationVolume
    193 Nb32f5f6c5333431bb375acb5b0cc5d14 rdf:first sg:person.01157753220.26
    194 rdf:rest Nd49b6f8dfc514b98be05761a5d4bb15f
    195 Nc66b01d95b7f464a958e0dfc4462ebb1 rdf:first sg:person.01067252435.53
    196 rdf:rest Ncdfd8ea3ac174c849e487692780494d1
    197 Ncdfd8ea3ac174c849e487692780494d1 rdf:first sg:person.01106620303.63
    198 rdf:rest N24931cf1a20c4103a8505d9d5b763369
    199 Nd49b6f8dfc514b98be05761a5d4bb15f rdf:first sg:person.01006004720.78
    200 rdf:rest Ne80f8308bcca4d1a9cd01c51164f16cd
    201 Nd5b0073c3c3a4ddf868a5c65019b519f rdf:first sg:person.0702056330.02
    202 rdf:rest N36ae3379beb5466694e3d1282b5f5f4f
    203 Ne80f8308bcca4d1a9cd01c51164f16cd rdf:first sg:person.012204604131.85
    204 rdf:rest Nc66b01d95b7f464a958e0dfc4462ebb1
    205 Nf04e48a2940d4569abce8f79c38a1c1f rdf:first sg:person.01235472333.35
    206 rdf:rest rdf:nil
    207 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    208 schema:name Medical and Health Sciences
    209 rdf:type schema:DefinedTerm
    210 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
    211 schema:name Clinical Sciences
    212 rdf:type schema:DefinedTerm
    213 sg:grant.9011173 http://pending.schema.org/fundedItem sg:pub.10.1186/s42523-019-0007-1
    214 rdf:type schema:MonetaryGrant
    215 sg:grant.9011955 http://pending.schema.org/fundedItem sg:pub.10.1186/s42523-019-0007-1
    216 rdf:type schema:MonetaryGrant
    217 sg:journal.1336340 schema:issn 2524-4671
    218 schema:name Animal Microbiome
    219 schema:publisher Springer Nature
    220 rdf:type schema:Periodical
    221 sg:person.01006004720.78 schema:affiliation grid-institutes:grid.5254.6
    222 schema:familyName Madsen
    223 schema:givenName Andreas Nygaard
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006004720.78
    225 rdf:type schema:Person
    226 sg:person.01041203677.36 schema:affiliation grid-institutes:None
    227 schema:familyName Kashani
    228 schema:givenName Alireza
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041203677.36
    230 rdf:type schema:Person
    231 sg:person.01067252435.53 schema:affiliation grid-institutes:grid.38142.3c
    232 schema:familyName Gerber
    233 schema:givenName Georg K.
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067252435.53
    235 rdf:type schema:Person
    236 sg:person.01106620303.63 schema:affiliation grid-institutes:grid.4973.9
    237 schema:familyName Holm
    238 schema:givenName Jens-Christian
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106620303.63
    240 rdf:type schema:Person
    241 sg:person.01157753220.26 schema:affiliation grid-institutes:grid.5254.6
    242 schema:familyName Kern
    243 schema:givenName Timo
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157753220.26
    245 rdf:type schema:Person
    246 sg:person.012204604131.85 schema:affiliation grid-institutes:grid.4973.9
    247 schema:familyName Holm
    248 schema:givenName Louise Aas
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012204604131.85
    250 rdf:type schema:Person
    251 sg:person.01235472333.35 schema:affiliation grid-institutes:grid.10825.3e
    252 schema:familyName Arumugam
    253 schema:givenName Manimozhiyan
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235472333.35
    255 rdf:type schema:Person
    256 sg:person.01262662636.78 schema:affiliation grid-institutes:grid.5254.6
    257 schema:familyName Jin
    258 schema:givenName Chunyu
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262662636.78
    260 rdf:type schema:Person
    261 sg:person.016350427407.47 schema:affiliation grid-institutes:grid.5254.6
    262 schema:familyName Hansen
    263 schema:givenName Torben
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016350427407.47
    265 rdf:type schema:Person
    266 sg:person.0702056330.02 schema:affiliation grid-institutes:grid.5254.6
    267 schema:familyName Brejnrod
    268 schema:givenName Asker Daniel
    269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702056330.02
    270 rdf:type schema:Person
    271 sg:person.0721620336.76 schema:affiliation grid-institutes:grid.5254.6
    272 schema:familyName Holst
    273 schema:givenName Birgitte
    274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721620336.76
    275 rdf:type schema:Person
    276 sg:pub.10.1038/ismej.2010.133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042916034
    277 https://doi.org/10.1038/ismej.2010.133
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/ismej.2014.45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051037244
    280 https://doi.org/10.1038/ismej.2014.45
    281 rdf:type schema:CreativeWork
    282 sg:pub.10.1038/ismej.2016.151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017331773
    283 https://doi.org/10.1038/ismej.2016.151
    284 rdf:type schema:CreativeWork
    285 sg:pub.10.1038/ismej.2017.119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090852589
    286 https://doi.org/10.1038/ismej.2017.119
    287 rdf:type schema:CreativeWork
    288 sg:pub.10.1038/nature05414 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023893418
    289 https://doi.org/10.1038/nature05414
    290 rdf:type schema:CreativeWork
    291 sg:pub.10.1038/nature07540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030170002
    292 https://doi.org/10.1038/nature07540
    293 rdf:type schema:CreativeWork
    294 sg:pub.10.1038/nature11450 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004546178
    295 https://doi.org/10.1038/nature11450
    296 rdf:type schema:CreativeWork
    297 sg:pub.10.1038/nature12198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002791386
    298 https://doi.org/10.1038/nature12198
    299 rdf:type schema:CreativeWork
    300 sg:pub.10.1038/nature12506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003823032
    301 https://doi.org/10.1038/nature12506
    302 rdf:type schema:CreativeWork
    303 sg:pub.10.1038/nature12820 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013830289
    304 https://doi.org/10.1038/nature12820
    305 rdf:type schema:CreativeWork
    306 sg:pub.10.1038/nature18646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017924056
    307 https://doi.org/10.1038/nature18646
    308 rdf:type schema:CreativeWork
    309 sg:pub.10.1038/nbt.3353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049321285
    310 https://doi.org/10.1038/nbt.3353
    311 rdf:type schema:CreativeWork
    312 sg:pub.10.1038/nmeth.3869 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016631324
    313 https://doi.org/10.1038/nmeth.3869
    314 rdf:type schema:CreativeWork
    315 sg:pub.10.1038/nmeth.f.303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009032055
    316 https://doi.org/10.1038/nmeth.f.303
    317 rdf:type schema:CreativeWork
    318 sg:pub.10.1038/srep16643 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023137628
    319 https://doi.org/10.1038/srep16643
    320 rdf:type schema:CreativeWork
    321 sg:pub.10.1186/ar2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006944697
    322 https://doi.org/10.1186/ar2004
    323 rdf:type schema:CreativeWork
    324 sg:pub.10.1186/s12866-015-0515-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006218857
    325 https://doi.org/10.1186/s12866-015-0515-4
    326 rdf:type schema:CreativeWork
    327 sg:pub.10.1186/s13059-014-0550-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015222646
    328 https://doi.org/10.1186/s13059-014-0550-8
    329 rdf:type schema:CreativeWork
    330 sg:pub.10.1186/s13073-016-0312-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015450395
    331 https://doi.org/10.1186/s13073-016-0312-1
    332 rdf:type schema:CreativeWork
    333 sg:pub.10.1186/s40168-017-0237-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1084252802
    334 https://doi.org/10.1186/s40168-017-0237-y
    335 rdf:type schema:CreativeWork
    336 sg:pub.10.1186/s40168-019-0637-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112223842
    337 https://doi.org/10.1186/s40168-019-0637-2
    338 rdf:type schema:CreativeWork
    339 grid-institutes:None schema:alternateName Qbiom Microbiome Consultancy Service, Copenhagen, Denmark
    340 schema:name Danish Diabetes Academy, Odense, Denmark
    341 Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark
    342 Qbiom Microbiome Consultancy Service, Copenhagen, Denmark
    343 rdf:type schema:Organization
    344 grid-institutes:grid.10825.3e schema:alternateName Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark
    345 schema:name Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark
    346 Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark
    347 rdf:type schema:Organization
    348 grid-institutes:grid.38142.3c schema:alternateName Department of Pathology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, USA
    349 schema:name Department of Pathology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, USA
    350 rdf:type schema:Organization
    351 grid-institutes:grid.4973.9 schema:alternateName The Children’s Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holbæk, 4300, Holbæk, Denmark
    352 schema:name Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark
    353 The Children’s Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holbæk, 4300, Holbæk, Denmark
    354 rdf:type schema:Organization
    355 grid-institutes:grid.5254.6 schema:alternateName Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark
    356 Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark
    357 schema:name Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Denmark
    358 Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark
    359 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...