Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Nguyet Kong, Whitney Ng, Kao Thao, Regina Agulto, Allison Weis, Kristi Spittle Kim, Jonas Korlach, Luke Hickey, Lenore Kelly, Stephen Lappin, Bart C. Weimer

ABSTRACT

BACKGROUND: The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to ensure the size and amounts of the sheared DNA fragments and final library. FINDINGS: The library construction automation was accomplished using the Agilent NGS workstation with Bravo accessories for heating, shaking, cooling, and magnetic bead manipulations for template purification. The quality control methods from gDNA input to final library using the Agilent Bioanalyzer System and Agilent TapeStation System were evaluated. CONCLUSIONS: Automated protocols of PacBio 10 kb library preparation produced libraries with similar technical performance to those generated manually. The TapeStation System proved to be a reliable method that could be used in a 96-well plate format to QC the DNA equivalent to the standard Bioanalyzer System results. The DNA Integrity Number that is calculated in the TapeStation System software upon analysis of genomic DNA is quite helpful to assure that the starting genomic DNA is not degraded. In this respect, the gDNA assay on the TapeStation System is preferable to the DNA 12000 assay on the Bioanalyzer System, which cannot run genomic DNA, nor can the Bioanalyzer work directly from the 96-well plates. More... »

PAGES

27

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s40793-017-0239-1

DOI

http://dx.doi.org/10.1186/s40793-017-0239-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084253223

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28344744


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, Davis", 
          "id": "https://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "Population Health and Reproduction Department, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kong", 
        "givenName": "Nguyet", 
        "id": "sg:person.0735316130.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735316130.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Roche (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418158.1", 
          "name": [
            "Genentech, S. San Francisco, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ng", 
        "givenName": "Whitney", 
        "id": "sg:person.07475110767.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07475110767.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Francisco", 
          "id": "https://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "University of California-San Francisco, San Francisco, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thao", 
        "givenName": "Kao", 
        "id": "sg:person.016200162167.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016200162167.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Davis", 
          "id": "https://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "Population Health and Reproduction Department, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Agulto", 
        "givenName": "Regina", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Davis", 
          "id": "https://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "Population Health and Reproduction Department, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weis", 
        "givenName": "Allison", 
        "id": "sg:person.011240711651.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011240711651.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pacific Biosciences (United States)", 
          "id": "https://www.grid.ac/institutes/grid.423340.2", 
          "name": [
            "Pacific Biosciences, Menlo Park, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Kristi Spittle", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pacific Biosciences (United States)", 
          "id": "https://www.grid.ac/institutes/grid.423340.2", 
          "name": [
            "Pacific Biosciences, Menlo Park, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Korlach", 
        "givenName": "Jonas", 
        "id": "sg:person.0610430600.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610430600.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pacific Biosciences (United States)", 
          "id": "https://www.grid.ac/institutes/grid.423340.2", 
          "name": [
            "Pacific Biosciences, Menlo Park, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hickey", 
        "givenName": "Luke", 
        "id": "sg:person.011303312233.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011303312233.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Agilent Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.422638.9", 
          "name": [
            "Agilent Technologies, Inc., Santa Clara, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kelly", 
        "givenName": "Lenore", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Agilent Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.422638.9", 
          "name": [
            "Agilent Technologies, Inc., Santa Clara, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lappin", 
        "givenName": "Stephen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Davis", 
          "id": "https://www.grid.ac/institutes/grid.27860.3b", 
          "name": [
            "Population Health and Reproduction Department, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weimer", 
        "givenName": "Bart C.", 
        "id": "sg:person.011527026767.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011527026767.02"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1520/jfs14351j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083313629"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "BACKGROUND: The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to ensure the size and amounts of the sheared DNA fragments and final library.\nFINDINGS: The library construction automation was accomplished using the Agilent NGS workstation with Bravo accessories for heating, shaking, cooling, and magnetic bead manipulations for template purification. The quality control methods from gDNA input to final library using the Agilent Bioanalyzer System and Agilent TapeStation System were evaluated.\nCONCLUSIONS: Automated protocols of PacBio 10 kb library preparation produced libraries with similar technical performance to those generated manually. The TapeStation System proved to be a reliable method that could be used in a 96-well plate format to QC the DNA equivalent to the standard Bioanalyzer System results. The DNA Integrity Number that is calculated in the TapeStation System software upon analysis of genomic DNA is quite helpful to assure that the starting genomic DNA is not degraded. In this respect, the gDNA assay on the TapeStation System is preferable to the DNA 12000 assay on the Bioanalyzer System, which cannot run genomic DNA, nor can the Bioanalyzer work directly from the 96-well plates.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s40793-017-0239-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2690739", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2528195", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2696402", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043441", 
        "issn": [
          "1944-3277"
        ], 
        "name": "Standards in Genomic Sciences", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing", 
    "pagination": "27", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9ccb270cb18bf5036e7151af378a21b6c2b0a6226ed408144308dbb452ea93d8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28344744"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101530505"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s40793-017-0239-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084253223"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s40793-017-0239-1", 
      "https://app.dimensions.ai/details/publication/pub.1084253223"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113655_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs40793-017-0239-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s40793-017-0239-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s40793-017-0239-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s40793-017-0239-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s40793-017-0239-1'


 

This table displays all metadata directly associated to this object as RDF triples.

155 TRIPLES      21 PREDICATES      30 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s40793-017-0239-1 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N24b6ee82129341ff8f8126510e1e15ee
4 schema:citation https://doi.org/10.1520/jfs14351j
5 schema:datePublished 2017-12
6 schema:datePublishedReg 2017-12-01
7 schema:description BACKGROUND: The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to ensure the size and amounts of the sheared DNA fragments and final library. FINDINGS: The library construction automation was accomplished using the Agilent NGS workstation with Bravo accessories for heating, shaking, cooling, and magnetic bead manipulations for template purification. The quality control methods from gDNA input to final library using the Agilent Bioanalyzer System and Agilent TapeStation System were evaluated. CONCLUSIONS: Automated protocols of PacBio 10 kb library preparation produced libraries with similar technical performance to those generated manually. The TapeStation System proved to be a reliable method that could be used in a 96-well plate format to QC the DNA equivalent to the standard Bioanalyzer System results. The DNA Integrity Number that is calculated in the TapeStation System software upon analysis of genomic DNA is quite helpful to assure that the starting genomic DNA is not degraded. In this respect, the gDNA assay on the TapeStation System is preferable to the DNA 12000 assay on the Bioanalyzer System, which cannot run genomic DNA, nor can the Bioanalyzer work directly from the 96-well plates.
8 schema:genre research_article
9 schema:inLanguage en
10 schema:isAccessibleForFree true
11 schema:isPartOf Nda816dce9ddd4bf08b2d4c7745f73aa5
12 Nfb22c2f2d8b2436e86e385fa4fb39f12
13 sg:journal.1043441
14 schema:name Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
15 schema:pagination 27
16 schema:productId N22ca7b97d46d4c8aa9435c9a718ca92b
17 N7947e3084063478c83b86eb904a52ca5
18 N7c34d8681bbf4384bcceb17bfc96df2b
19 Nc9d7935b8e34435ab62b73a21156da02
20 Nce8ceb6f23ff46169fc249e8fd1d48e5
21 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084253223
22 https://doi.org/10.1186/s40793-017-0239-1
23 schema:sdDatePublished 2019-04-11T10:32
24 schema:sdLicense https://scigraph.springernature.com/explorer/license/
25 schema:sdPublisher Nf4b1cda9b16145f9a8d5aaf79257eecb
26 schema:url https://link.springer.com/10.1186%2Fs40793-017-0239-1
27 sgo:license sg:explorer/license/
28 sgo:sdDataset articles
29 rdf:type schema:ScholarlyArticle
30 N08e0f58c9a3a453294c56d74329aa236 rdf:first N6983863f652f497cb12bad4019df6469
31 rdf:rest N12696bea34f64797a563eb90348097fc
32 N12696bea34f64797a563eb90348097fc rdf:first sg:person.011527026767.02
33 rdf:rest rdf:nil
34 N22ca7b97d46d4c8aa9435c9a718ca92b schema:name nlm_unique_id
35 schema:value 101530505
36 rdf:type schema:PropertyValue
37 N24b6ee82129341ff8f8126510e1e15ee rdf:first sg:person.0735316130.50
38 rdf:rest Nb488fbd9dc494ca1ad64cec56cd8e30b
39 N2f4987d7f24c45968ee884ea26338c0a schema:affiliation https://www.grid.ac/institutes/grid.422638.9
40 schema:familyName Kelly
41 schema:givenName Lenore
42 rdf:type schema:Person
43 N3421e35a26ab4d878601c4c1dd41b839 rdf:first sg:person.016200162167.73
44 rdf:rest Ncef3b3c21d5b409889a7e5c955549814
45 N42a37c95238f493da2282c5458a68e1f schema:affiliation https://www.grid.ac/institutes/grid.423340.2
46 schema:familyName Kim
47 schema:givenName Kristi Spittle
48 rdf:type schema:Person
49 N5e52dc0e995a456eb3548439ed389a7c rdf:first sg:person.011240711651.24
50 rdf:rest Nec06d8302d4d4c7ba6d5a835f4de1730
51 N6983863f652f497cb12bad4019df6469 schema:affiliation https://www.grid.ac/institutes/grid.422638.9
52 schema:familyName Lappin
53 schema:givenName Stephen
54 rdf:type schema:Person
55 N752537468a79490fb83f2b9e454ba3ab rdf:first sg:person.0610430600.26
56 rdf:rest Ncabe83883274433ba062b730540c2d76
57 N7947e3084063478c83b86eb904a52ca5 schema:name doi
58 schema:value 10.1186/s40793-017-0239-1
59 rdf:type schema:PropertyValue
60 N7c34d8681bbf4384bcceb17bfc96df2b schema:name dimensions_id
61 schema:value pub.1084253223
62 rdf:type schema:PropertyValue
63 Nb488fbd9dc494ca1ad64cec56cd8e30b rdf:first sg:person.07475110767.92
64 rdf:rest N3421e35a26ab4d878601c4c1dd41b839
65 Nc9d7935b8e34435ab62b73a21156da02 schema:name readcube_id
66 schema:value 9ccb270cb18bf5036e7151af378a21b6c2b0a6226ed408144308dbb452ea93d8
67 rdf:type schema:PropertyValue
68 Ncabe83883274433ba062b730540c2d76 rdf:first sg:person.011303312233.33
69 rdf:rest Nd2b6f00ce1284316811847060b0d8f59
70 Nce8ceb6f23ff46169fc249e8fd1d48e5 schema:name pubmed_id
71 schema:value 28344744
72 rdf:type schema:PropertyValue
73 Ncef3b3c21d5b409889a7e5c955549814 rdf:first Ne59f0a4baeeb456692640a0a6f201b96
74 rdf:rest N5e52dc0e995a456eb3548439ed389a7c
75 Nd2b6f00ce1284316811847060b0d8f59 rdf:first N2f4987d7f24c45968ee884ea26338c0a
76 rdf:rest N08e0f58c9a3a453294c56d74329aa236
77 Nda816dce9ddd4bf08b2d4c7745f73aa5 schema:volumeNumber 12
78 rdf:type schema:PublicationVolume
79 Ne59f0a4baeeb456692640a0a6f201b96 schema:affiliation https://www.grid.ac/institutes/grid.27860.3b
80 schema:familyName Agulto
81 schema:givenName Regina
82 rdf:type schema:Person
83 Nec06d8302d4d4c7ba6d5a835f4de1730 rdf:first N42a37c95238f493da2282c5458a68e1f
84 rdf:rest N752537468a79490fb83f2b9e454ba3ab
85 Nf4b1cda9b16145f9a8d5aaf79257eecb schema:name Springer Nature - SN SciGraph project
86 rdf:type schema:Organization
87 Nfb22c2f2d8b2436e86e385fa4fb39f12 schema:issueNumber 1
88 rdf:type schema:PublicationIssue
89 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
90 schema:name Biological Sciences
91 rdf:type schema:DefinedTerm
92 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
93 schema:name Genetics
94 rdf:type schema:DefinedTerm
95 sg:grant.2528195 http://pending.schema.org/fundedItem sg:pub.10.1186/s40793-017-0239-1
96 rdf:type schema:MonetaryGrant
97 sg:grant.2690739 http://pending.schema.org/fundedItem sg:pub.10.1186/s40793-017-0239-1
98 rdf:type schema:MonetaryGrant
99 sg:grant.2696402 http://pending.schema.org/fundedItem sg:pub.10.1186/s40793-017-0239-1
100 rdf:type schema:MonetaryGrant
101 sg:journal.1043441 schema:issn 1944-3277
102 schema:name Standards in Genomic Sciences
103 rdf:type schema:Periodical
104 sg:person.011240711651.24 schema:affiliation https://www.grid.ac/institutes/grid.27860.3b
105 schema:familyName Weis
106 schema:givenName Allison
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011240711651.24
108 rdf:type schema:Person
109 sg:person.011303312233.33 schema:affiliation https://www.grid.ac/institutes/grid.423340.2
110 schema:familyName Hickey
111 schema:givenName Luke
112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011303312233.33
113 rdf:type schema:Person
114 sg:person.011527026767.02 schema:affiliation https://www.grid.ac/institutes/grid.27860.3b
115 schema:familyName Weimer
116 schema:givenName Bart C.
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011527026767.02
118 rdf:type schema:Person
119 sg:person.016200162167.73 schema:affiliation https://www.grid.ac/institutes/grid.266102.1
120 schema:familyName Thao
121 schema:givenName Kao
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016200162167.73
123 rdf:type schema:Person
124 sg:person.0610430600.26 schema:affiliation https://www.grid.ac/institutes/grid.423340.2
125 schema:familyName Korlach
126 schema:givenName Jonas
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610430600.26
128 rdf:type schema:Person
129 sg:person.0735316130.50 schema:affiliation https://www.grid.ac/institutes/grid.27860.3b
130 schema:familyName Kong
131 schema:givenName Nguyet
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735316130.50
133 rdf:type schema:Person
134 sg:person.07475110767.92 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
135 schema:familyName Ng
136 schema:givenName Whitney
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07475110767.92
138 rdf:type schema:Person
139 https://doi.org/10.1520/jfs14351j schema:sameAs https://app.dimensions.ai/details/publication/pub.1083313629
140 rdf:type schema:CreativeWork
141 https://www.grid.ac/institutes/grid.266102.1 schema:alternateName University of California, San Francisco
142 schema:name University of California-San Francisco, San Francisco, CA, USA
143 rdf:type schema:Organization
144 https://www.grid.ac/institutes/grid.27860.3b schema:alternateName University of California, Davis
145 schema:name Population Health and Reproduction Department, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA
146 rdf:type schema:Organization
147 https://www.grid.ac/institutes/grid.418158.1 schema:alternateName Roche (United States)
148 schema:name Genentech, S. San Francisco, CA, USA
149 rdf:type schema:Organization
150 https://www.grid.ac/institutes/grid.422638.9 schema:alternateName Agilent Technologies (United States)
151 schema:name Agilent Technologies, Inc., Santa Clara, CA, USA
152 rdf:type schema:Organization
153 https://www.grid.ac/institutes/grid.423340.2 schema:alternateName Pacific Biosciences (United States)
154 schema:name Pacific Biosciences, Menlo Park, CA, USA
155 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...