Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-02-01

AUTHORS

Marisol Orozco-Ibarra, Jorge Muñoz-Sánchez, Martín E. Zavala-Medina, Benjamín Pineda, Roxana Magaña-Maldonado, Edgar Vázquez-Contreras, Perla D. Maldonado, José Pedraza-Chaverri, María Elena Chánez-Cárdenas

ABSTRACT

BackgroundAged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-1α) and up-regulation of HIF-1α-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells.ResultsWe found that CoCl2 induced the stabilization of HIF-1α and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1α stabilization, activity not previously reported for AGE and SAC.ConclusionsObtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1α and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditions. More... »

PAGES

7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s40659-016-0067-6

DOI

http://dx.doi.org/10.1186/s40659-016-0067-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038027523

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26830333


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28 schema:description BackgroundAged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-1α) and up-regulation of HIF-1α-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells.ResultsWe found that CoCl2 induced the stabilization of HIF-1α and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1α stabilization, activity not previously reported for AGE and SAC.ConclusionsObtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1α and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditions.
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37 ConclusionsObtained results
38 HIF-1α
39 HIF-1α stabilization
40 PC12 cells
41 ROS
42 ResultsWe
43 S-allylcysteine
44 activation
45 activity
46 age
47 aged garlic extract
48 alternative
49 antioxidants
50 apoptosis
51 apoptotic cell death
52 attenuation
53 cancer
54 cell death
55 cells
56 cerebral ischemia
57 chemical hypoxia model
58 chloride
59 cobalt chloride
60 compounds
61 concentration
62 conditions
63 death
64 dependent genes
65 effect
66 effect of age
67 events
68 extent
69 extract
70 factors
71 garlic extract
72 generation
73 genes
74 hypoxia extent
75 hypoxia model
76 hypoxia stress
77 hypoxia-inducible factor
78 hypoxic conditions
79 incubation time
80 inducible factor
81 ischemia
82 later stages
83 localization
84 loss
85 membrane potential
86 mitochondrial membrane potential
87 model
88 natural antioxidants
89 natural compounds
90 nuclear localization
91 number
92 number of cells
93 oxygen species generation
94 potential
95 present study
96 protection
97 protective effect
98 reactive oxygen species generation
99 regulation
100 results
101 species generation
102 stabilization
103 stage
104 stress
105 study
106 subunits
107 therapeutic alternative
108 time
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