Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Md. Shafiqur Rahman, Chunbao Charles Xu, Wensheng Qin

ABSTRACT

The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate. A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli–K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli. More... »

PAGES

3

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s40643-018-0189-5

DOI

http://dx.doi.org/10.1186/s40643-018-0189-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1100482132


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Chittagong", 
          "id": "https://www.grid.ac/institutes/grid.413089.7", 
          "name": [
            "Department of Biology, Lakehead University, 955 Oliver Road, P7B 5E1, Thunder Bay, ON, Canada", 
            "Department of Microbiology, University of Chittagong, Chittagong, Bangladesh"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rahman", 
        "givenName": "Md. Shafiqur", 
        "id": "sg:person.010007326347.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010007326347.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Western University", 
          "id": "https://www.grid.ac/institutes/grid.39381.30", 
          "name": [
            "Institute for Chemicals and Fuels from Alternative Resources (ICFAR), Western University, N6A 5B9, London, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Chunbao Charles", 
        "id": "sg:person.0724354206.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724354206.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lakehead University", 
          "id": "https://www.grid.ac/institutes/grid.258900.6", 
          "name": [
            "Department of Biology, Lakehead University, 955 Oliver Road, P7B 5E1, Thunder Bay, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qin", 
        "givenName": "Wensheng", 
        "id": "sg:person.01330031462.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330031462.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00253-011-3173-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000645750", 
          "https://doi.org/10.1007/s00253-011-3173-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2012.02.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002987126"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-013-4959-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005958704", 
          "https://doi.org/10.1007/s00253-013-4959-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2672.2008.04046.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006015102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b913501d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008056084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b913501d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008056084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2011.05.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008299218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2011.08.097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009462755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biotechadv.2009.05.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009688560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2014.02.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012474464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<544::aid-bit3>3.0.co;2-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012554985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cssc.201000306", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013003061"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biotechadv.2011.01.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014933899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2672.2000.01211.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016348036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1213024110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019363849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00449-003-0338-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020707314", 
          "https://doi.org/10.1007/s00449-003-0338-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2009.03.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025548825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-007-1003-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025963315", 
          "https://doi.org/10.1007/s00253-007-1003-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-007-1003-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025963315", 
          "https://doi.org/10.1007/s00253-007-1003-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2014.02.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028321160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13068-015-0324-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030179363", 
          "https://doi.org/10.1186/s13068-015-0324-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2007.08.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032101037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1754-6834-5-88", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039582467", 
          "https://doi.org/10.1186/1754-6834-5-88"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2014.11.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042542869"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1754-6834-6-60", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043408611", 
          "https://doi.org/10.1186/1754-6834-6-60"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.24427", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044090609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/biot.200600032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045643887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2012.08.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045843346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2013.07.081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045944985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1106958108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049724159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00348-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052469727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7150/ijbs.17594", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084480841"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate. A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli\u2013K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s40643-018-0189-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1052294", 
        "issn": [
          "2197-4365"
        ], 
        "name": "Bioresources and Bioprocessing", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol", 
    "pagination": "3", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9f260e9afbc0fa69571da893e711727317ff15d01886bc06618aa26234fe2521"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s40643-018-0189-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1100482132"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s40643-018-0189-5", 
      "https://app.dimensions.ai/details/publication/pub.1100482132"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000484.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186/s40643-018-0189-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s40643-018-0189-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s40643-018-0189-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s40643-018-0189-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s40643-018-0189-5'


 

This table displays all metadata directly associated to this object as RDF triples.

188 TRIPLES      21 PREDICATES      60 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s40643-018-0189-5 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N9286ffae5b504c52b36680dc924d7cb0
4 schema:citation sg:pub.10.1007/s00253-007-1003-z
5 sg:pub.10.1007/s00253-011-3173-y
6 sg:pub.10.1007/s00253-013-4959-x
7 sg:pub.10.1007/s00449-003-0338-9
8 sg:pub.10.1038/227680a0
9 sg:pub.10.1186/1754-6834-5-88
10 sg:pub.10.1186/1754-6834-6-60
11 sg:pub.10.1186/s13068-015-0324-x
12 https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<544::aid-bit3>3.0.co;2-a
13 https://doi.org/10.1002/biot.200600032
14 https://doi.org/10.1002/bit.24427
15 https://doi.org/10.1002/cssc.201000306
16 https://doi.org/10.1006/meth.2001.1262
17 https://doi.org/10.1016/0003-2697(76)90527-3
18 https://doi.org/10.1016/j.biortech.2009.03.027
19 https://doi.org/10.1016/j.biortech.2011.08.097
20 https://doi.org/10.1016/j.biortech.2013.07.081
21 https://doi.org/10.1016/j.biotechadv.2009.05.002
22 https://doi.org/10.1016/j.biotechadv.2011.01.007
23 https://doi.org/10.1016/j.copbio.2011.05.005
24 https://doi.org/10.1016/j.cub.2012.02.002
25 https://doi.org/10.1016/j.ymben.2007.08.003
26 https://doi.org/10.1016/j.ymben.2012.08.001
27 https://doi.org/10.1016/j.ymben.2014.02.003
28 https://doi.org/10.1016/j.ymben.2014.02.004
29 https://doi.org/10.1016/j.ymben.2014.11.010
30 https://doi.org/10.1039/b913501d
31 https://doi.org/10.1046/j.1365-2672.2000.01211.x
32 https://doi.org/10.1073/pnas.1106958108
33 https://doi.org/10.1073/pnas.1213024110
34 https://doi.org/10.1111/j.1365-2672.2008.04046.x
35 https://doi.org/10.1128/jb.00348-07
36 https://doi.org/10.7150/ijbs.17594
37 schema:datePublished 2018-12
38 schema:datePublishedReg 2018-12-01
39 schema:description The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate. A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli–K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree true
43 schema:isPartOf N95788106a34b449a9f1c9e3ddf37ea2a
44 Na3f8a970bc1242b095d8c9de0180eeca
45 sg:journal.1052294
46 schema:name Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol
47 schema:pagination 3
48 schema:productId N0e874c5bf2f148759688f71006028288
49 N57dad817206c40af90ce79c13904ef16
50 N8321f0e5ae234f7f9bce468a4ba4f1cf
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100482132
52 https://doi.org/10.1186/s40643-018-0189-5
53 schema:sdDatePublished 2019-04-10T19:51
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher Nbdbc0c988bb44ca49a70e2a3dd95c21d
56 schema:url http://link.springer.com/10.1186/s40643-018-0189-5
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N0e874c5bf2f148759688f71006028288 schema:name dimensions_id
61 schema:value pub.1100482132
62 rdf:type schema:PropertyValue
63 N57dad817206c40af90ce79c13904ef16 schema:name doi
64 schema:value 10.1186/s40643-018-0189-5
65 rdf:type schema:PropertyValue
66 N6bf3b7a3010442e5ab341deef98e6d9d rdf:first sg:person.01330031462.51
67 rdf:rest rdf:nil
68 N8321f0e5ae234f7f9bce468a4ba4f1cf schema:name readcube_id
69 schema:value 9f260e9afbc0fa69571da893e711727317ff15d01886bc06618aa26234fe2521
70 rdf:type schema:PropertyValue
71 N9286ffae5b504c52b36680dc924d7cb0 rdf:first sg:person.010007326347.07
72 rdf:rest Nd9990df5ab414ac2836c1c761c598782
73 N95788106a34b449a9f1c9e3ddf37ea2a schema:issueNumber 1
74 rdf:type schema:PublicationIssue
75 Na3f8a970bc1242b095d8c9de0180eeca schema:volumeNumber 5
76 rdf:type schema:PublicationVolume
77 Nbdbc0c988bb44ca49a70e2a3dd95c21d schema:name Springer Nature - SN SciGraph project
78 rdf:type schema:Organization
79 Nd9990df5ab414ac2836c1c761c598782 rdf:first sg:person.0724354206.69
80 rdf:rest N6bf3b7a3010442e5ab341deef98e6d9d
81 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
82 schema:name Biological Sciences
83 rdf:type schema:DefinedTerm
84 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
85 schema:name Genetics
86 rdf:type schema:DefinedTerm
87 sg:journal.1052294 schema:issn 2197-4365
88 schema:name Bioresources and Bioprocessing
89 rdf:type schema:Periodical
90 sg:person.010007326347.07 schema:affiliation https://www.grid.ac/institutes/grid.413089.7
91 schema:familyName Rahman
92 schema:givenName Md. Shafiqur
93 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010007326347.07
94 rdf:type schema:Person
95 sg:person.01330031462.51 schema:affiliation https://www.grid.ac/institutes/grid.258900.6
96 schema:familyName Qin
97 schema:givenName Wensheng
98 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330031462.51
99 rdf:type schema:Person
100 sg:person.0724354206.69 schema:affiliation https://www.grid.ac/institutes/grid.39381.30
101 schema:familyName Xu
102 schema:givenName Chunbao Charles
103 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724354206.69
104 rdf:type schema:Person
105 sg:pub.10.1007/s00253-007-1003-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1025963315
106 https://doi.org/10.1007/s00253-007-1003-z
107 rdf:type schema:CreativeWork
108 sg:pub.10.1007/s00253-011-3173-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1000645750
109 https://doi.org/10.1007/s00253-011-3173-y
110 rdf:type schema:CreativeWork
111 sg:pub.10.1007/s00253-013-4959-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005958704
112 https://doi.org/10.1007/s00253-013-4959-x
113 rdf:type schema:CreativeWork
114 sg:pub.10.1007/s00449-003-0338-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020707314
115 https://doi.org/10.1007/s00449-003-0338-9
116 rdf:type schema:CreativeWork
117 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
118 https://doi.org/10.1038/227680a0
119 rdf:type schema:CreativeWork
120 sg:pub.10.1186/1754-6834-5-88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039582467
121 https://doi.org/10.1186/1754-6834-5-88
122 rdf:type schema:CreativeWork
123 sg:pub.10.1186/1754-6834-6-60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043408611
124 https://doi.org/10.1186/1754-6834-6-60
125 rdf:type schema:CreativeWork
126 sg:pub.10.1186/s13068-015-0324-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030179363
127 https://doi.org/10.1186/s13068-015-0324-x
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<544::aid-bit3>3.0.co;2-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1012554985
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1002/biot.200600032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045643887
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1002/bit.24427 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044090609
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1002/cssc.201000306 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013003061
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1006/meth.2001.1262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027621591
138 rdf:type schema:CreativeWork
139 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1016/j.biortech.2009.03.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025548825
142 rdf:type schema:CreativeWork
143 https://doi.org/10.1016/j.biortech.2011.08.097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009462755
144 rdf:type schema:CreativeWork
145 https://doi.org/10.1016/j.biortech.2013.07.081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045944985
146 rdf:type schema:CreativeWork
147 https://doi.org/10.1016/j.biotechadv.2009.05.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009688560
148 rdf:type schema:CreativeWork
149 https://doi.org/10.1016/j.biotechadv.2011.01.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014933899
150 rdf:type schema:CreativeWork
151 https://doi.org/10.1016/j.copbio.2011.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008299218
152 rdf:type schema:CreativeWork
153 https://doi.org/10.1016/j.cub.2012.02.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002987126
154 rdf:type schema:CreativeWork
155 https://doi.org/10.1016/j.ymben.2007.08.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032101037
156 rdf:type schema:CreativeWork
157 https://doi.org/10.1016/j.ymben.2012.08.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045843346
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/j.ymben.2014.02.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028321160
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.ymben.2014.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012474464
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.ymben.2014.11.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042542869
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1039/b913501d schema:sameAs https://app.dimensions.ai/details/publication/pub.1008056084
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1046/j.1365-2672.2000.01211.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016348036
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1073/pnas.1106958108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049724159
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1073/pnas.1213024110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019363849
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1111/j.1365-2672.2008.04046.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006015102
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1128/jb.00348-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052469727
176 rdf:type schema:CreativeWork
177 https://doi.org/10.7150/ijbs.17594 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084480841
178 rdf:type schema:CreativeWork
179 https://www.grid.ac/institutes/grid.258900.6 schema:alternateName Lakehead University
180 schema:name Department of Biology, Lakehead University, 955 Oliver Road, P7B 5E1, Thunder Bay, ON, Canada
181 rdf:type schema:Organization
182 https://www.grid.ac/institutes/grid.39381.30 schema:alternateName Western University
183 schema:name Institute for Chemicals and Fuels from Alternative Resources (ICFAR), Western University, N6A 5B9, London, ON, Canada
184 rdf:type schema:Organization
185 https://www.grid.ac/institutes/grid.413089.7 schema:alternateName University of Chittagong
186 schema:name Department of Biology, Lakehead University, 955 Oliver Road, P7B 5E1, Thunder Bay, ON, Canada
187 Department of Microbiology, University of Chittagong, Chittagong, Bangladesh
188 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...