Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12-04

AUTHORS

Tássia R. Costa, Sante E. I. Carone, Luiz F. F. Tucci, Danilo L. Menaldo, Nathalia G. Rosa-Garzon, Hamilton Cabral, Suely V. Sampaio

ABSTRACT

BackgroundL-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two Bothrops LAAOs, one isolated from Bothrops jararacussu (BjussuLAAO-II) and the other from Bothrops moojeni (BmooLAAO-I) venoms.Methods and resultsThe enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at different pH ranges and temperatures. BjussuLAAO-II and BmooLAAO-I demonstrated higher affinity for hydrophobic amino acids, such as Phe and Leu. The two enzymes showed high enzymatic activity in a wide temperature range, from 25 to 75 °C, and presented optimum pH around 7.0. Additionally, Zn2+, Al3+, Cu2+ and Ni2+ ions negatively modulated the enzymatic activity of both LAAOs. As to stability, BjussuLAAO-II and BmooLAAO-I showed high enzymatic activity for 42 days stored at 4 °C in neutral pH solution. Moreover, the glycan portions of both LAAOs were analyzed by capillary electrophoresis, which revealed that BjussuLAAO-II presented two main glycan portions with relative masses of 7.78 and 8.13 CGU, while BmooLAAO-I showed three portions of 7.58, 7.94 and 8.37 CGU.ConclusionsOur results showed that, when stored properly, BjussuLAAO-II and BmooLAAO-I present enzymatic stability over a long time period, which is very important to allow the use of these enzymes in pharmacological studies of great impact in the medical field. More... »

PAGES

37

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s40409-018-0172-9

DOI

http://dx.doi.org/10.1186/s40409-018-0172-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110370527

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30534149


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Costa", 
        "givenName": "T\u00e1ssia R.", 
        "id": "sg:person.01051030743.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051030743.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Carone", 
        "givenName": "Sante E. I.", 
        "id": "sg:person.07372115121.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07372115121.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tucci", 
        "givenName": "Luiz F. F.", 
        "id": "sg:person.014011333227.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014011333227.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Menaldo", 
        "givenName": "Danilo L.", 
        "id": "sg:person.01026117775.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026117775.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de Ci\u00eancias Farmac\u00eauticas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de Ci\u00eancias Farmac\u00eauticas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rosa-Garzon", 
        "givenName": "Nathalia G.", 
        "id": "sg:person.015352136017.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015352136017.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de Ci\u00eancias Farmac\u00eauticas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de Ci\u00eancias Farmac\u00eauticas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cabral", 
        "givenName": "Hamilton", 
        "id": "sg:person.0657321564.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657321564.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil", 
          "id": "http://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (FCFRP-USP), Avenida do Caf\u00e9, s/n, B. Monte Alegre, CEP 14040-903, Ribeir\u00e3o Preto, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sampaio", 
        "givenName": "Suely V.", 
        "id": "sg:person.0746137414.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746137414.79"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s10495-006-7959-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047343183", 
          "https://doi.org/10.1007/s10495-006-7959-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40409-015-0025-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043961926", 
          "https://doi.org/10.1186/s40409-015-0025-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40409-015-0026-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036525575", 
          "https://doi.org/10.1186/s40409-015-0026-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1678-9199-20-23", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000602197", 
          "https://doi.org/10.1186/1678-9199-20-23"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12-04", 
    "datePublishedReg": "2018-12-04", 
    "description": "BackgroundL-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two Bothrops LAAOs, one isolated from Bothrops jararacussu (BjussuLAAO-II) and the other from Bothrops moojeni (BmooLAAO-I) venoms.Methods and resultsThe enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at different pH ranges and temperatures. BjussuLAAO-II and BmooLAAO-I demonstrated higher affinity for hydrophobic amino acids, such as Phe and Leu. The two enzymes showed high enzymatic activity in a wide temperature range, from 25 to 75\u2009\u00b0C, and presented optimum pH around 7.0. Additionally, Zn2+, Al3+, Cu2+ and Ni2+ ions negatively modulated the enzymatic activity of both LAAOs. As to stability, BjussuLAAO-II and BmooLAAO-I showed high enzymatic activity for 42\u2009days stored at 4\u2009\u00b0C in neutral pH solution. Moreover, the glycan portions of both LAAOs were analyzed by capillary electrophoresis, which revealed that BjussuLAAO-II presented two main glycan portions with relative masses of 7.78 and 8.13 CGU, while BmooLAAO-I showed three portions of 7.58, 7.94 and 8.37 CGU.ConclusionsOur results showed that, when stored properly, BjussuLAAO-II and BmooLAAO-I present enzymatic stability over a long time period, which is very important to allow the use of these enzymes in pharmacological studies of great impact in the medical field.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s40409-018-0172-9", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.9106255", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4476820", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1357529", 
        "issn": [
          "1678-9180", 
          "1678-9199"
        ], 
        "name": "Journal of Venomous Animals and Toxins including Tropical Diseases", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "24"
      }
    ], 
    "keywords": [
      "BjussuLAAO-II", 
      "great therapeutic potential", 
      "microplate colorimetric assay", 
      "therapeutic potential", 
      "bacterial infections", 
      "pharmacological studies", 
      "Bothrops moojeni venom", 
      "ConclusionsOur results", 
      "moojeni venom", 
      "L-amino acid oxidase", 
      "enzymatic activity", 
      "snake venom", 
      "acid oxidase", 
      "BmooLAAO", 
      "venom", 
      "high affinity", 
      "enzymatic stability", 
      "colorimetric assay", 
      "cancer", 
      "infection", 
      "activity", 
      "disease", 
      "study", 
      "LAAOs", 
      "time period", 
      "amino acids", 
      "new strategy", 
      "enzyme", 
      "Bothrops", 
      "days", 
      "assays", 
      "high enzymatic activity", 
      "oxidase", 
      "glycan portion", 
      "portion", 
      "medical field", 
      "acid substrates", 
      "long time periods", 
      "hydrophobic amino acids", 
      "period", 
      "evaluation", 
      "relative mass", 
      "great impact", 
      "Phe", 
      "objective", 
      "brief evaluation", 
      "presence", 
      "acid", 
      "Leu", 
      "CGU", 
      "use", 
      "affinity", 
      "mass", 
      "stability studies", 
      "electrophoresis", 
      "strategies", 
      "impact", 
      "potential", 
      "investigation", 
      "main objective", 
      "results", 
      "tool", 
      "range", 
      "method", 
      "neutral pH solution", 
      "capillary electrophoresis", 
      "pH", 
      "stability", 
      "optimum pH", 
      "Zn2", 
      "substrate", 
      "field", 
      "Ni2", 
      "pH solution", 
      "solution", 
      "ions", 
      "kinetic investigation", 
      "different ions", 
      "Cu2", 
      "temperature", 
      "Al3", 
      "wide temperature range", 
      "temperature range"
    ], 
    "name": "Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases", 
    "pagination": "37", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1110370527"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s40409-018-0172-9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30534149"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s40409-018-0172-9", 
      "https://app.dimensions.ai/details/publication/pub.1110370527"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-11-24T21:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_788.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s40409-018-0172-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s40409-018-0172-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s40409-018-0172-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s40409-018-0172-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s40409-018-0172-9'


 

This table displays all metadata directly associated to this object as RDF triples.

208 TRIPLES      21 PREDICATES      112 URIs      100 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s40409-018-0172-9 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N7d5d5bf95d63456ebf6e5678e73d6cb2
4 schema:citation sg:pub.10.1007/s10495-006-7959-9
5 sg:pub.10.1186/1678-9199-20-23
6 sg:pub.10.1186/s40409-015-0025-8
7 sg:pub.10.1186/s40409-015-0026-7
8 schema:datePublished 2018-12-04
9 schema:datePublishedReg 2018-12-04
10 schema:description BackgroundL-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two Bothrops LAAOs, one isolated from Bothrops jararacussu (BjussuLAAO-II) and the other from Bothrops moojeni (BmooLAAO-I) venoms.Methods and resultsThe enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at different pH ranges and temperatures. BjussuLAAO-II and BmooLAAO-I demonstrated higher affinity for hydrophobic amino acids, such as Phe and Leu. The two enzymes showed high enzymatic activity in a wide temperature range, from 25 to 75 °C, and presented optimum pH around 7.0. Additionally, Zn2+, Al3+, Cu2+ and Ni2+ ions negatively modulated the enzymatic activity of both LAAOs. As to stability, BjussuLAAO-II and BmooLAAO-I showed high enzymatic activity for 42 days stored at 4 °C in neutral pH solution. Moreover, the glycan portions of both LAAOs were analyzed by capillary electrophoresis, which revealed that BjussuLAAO-II presented two main glycan portions with relative masses of 7.78 and 8.13 CGU, while BmooLAAO-I showed three portions of 7.58, 7.94 and 8.37 CGU.ConclusionsOur results showed that, when stored properly, BjussuLAAO-II and BmooLAAO-I present enzymatic stability over a long time period, which is very important to allow the use of these enzymes in pharmacological studies of great impact in the medical field.
11 schema:genre article
12 schema:isAccessibleForFree true
13 schema:isPartOf N2950e887eae148b29f406c2ff789b8cc
14 Nb25d20caf86c4adfb32a725bf21c958b
15 sg:journal.1357529
16 schema:keywords Al3
17 BjussuLAAO-II
18 BmooLAAO
19 Bothrops
20 Bothrops moojeni venom
21 CGU
22 ConclusionsOur results
23 Cu2
24 L-amino acid oxidase
25 LAAOs
26 Leu
27 Ni2
28 Phe
29 Zn2
30 acid
31 acid oxidase
32 acid substrates
33 activity
34 affinity
35 amino acids
36 assays
37 bacterial infections
38 brief evaluation
39 cancer
40 capillary electrophoresis
41 colorimetric assay
42 days
43 different ions
44 disease
45 electrophoresis
46 enzymatic activity
47 enzymatic stability
48 enzyme
49 evaluation
50 field
51 glycan portion
52 great impact
53 great therapeutic potential
54 high affinity
55 high enzymatic activity
56 hydrophobic amino acids
57 impact
58 infection
59 investigation
60 ions
61 kinetic investigation
62 long time periods
63 main objective
64 mass
65 medical field
66 method
67 microplate colorimetric assay
68 moojeni venom
69 neutral pH solution
70 new strategy
71 objective
72 optimum pH
73 oxidase
74 pH
75 pH solution
76 period
77 pharmacological studies
78 portion
79 potential
80 presence
81 range
82 relative mass
83 results
84 snake venom
85 solution
86 stability
87 stability studies
88 strategies
89 study
90 substrate
91 temperature
92 temperature range
93 therapeutic potential
94 time period
95 tool
96 use
97 venom
98 wide temperature range
99 schema:name Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases
100 schema:pagination 37
101 schema:productId N0bdfebeb891641cdbf302d72ef5110a2
102 N8aa429de9db940ec9315cfa4c20f8090
103 N93f86f7aa9db47ac8fdb27425573ee47
104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110370527
105 https://doi.org/10.1186/s40409-018-0172-9
106 schema:sdDatePublished 2022-11-24T21:04
107 schema:sdLicense https://scigraph.springernature.com/explorer/license/
108 schema:sdPublisher N05b8034da57040d6bb7a6264217f492f
109 schema:url https://doi.org/10.1186/s40409-018-0172-9
110 sgo:license sg:explorer/license/
111 sgo:sdDataset articles
112 rdf:type schema:ScholarlyArticle
113 N05b8034da57040d6bb7a6264217f492f schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 N0bdfebeb891641cdbf302d72ef5110a2 schema:name pubmed_id
116 schema:value 30534149
117 rdf:type schema:PropertyValue
118 N1b91151cb4db44bea9f70040d203502a rdf:first sg:person.014011333227.20
119 rdf:rest N66961ff2d9474dc8b01aa8f79cff4d47
120 N246d8d2ebf7a478ca50b184ac497ce9a rdf:first sg:person.015352136017.70
121 rdf:rest Neb5fbb2ff1ce4b468b7f22e759360f80
122 N2950e887eae148b29f406c2ff789b8cc schema:volumeNumber 24
123 rdf:type schema:PublicationVolume
124 N536f444b8657463d89d3e8f8e2099203 rdf:first sg:person.0746137414.79
125 rdf:rest rdf:nil
126 N66961ff2d9474dc8b01aa8f79cff4d47 rdf:first sg:person.01026117775.10
127 rdf:rest N246d8d2ebf7a478ca50b184ac497ce9a
128 N7d5d5bf95d63456ebf6e5678e73d6cb2 rdf:first sg:person.01051030743.43
129 rdf:rest Nfc146325eda34c029c1d0c960d6616e3
130 N8aa429de9db940ec9315cfa4c20f8090 schema:name dimensions_id
131 schema:value pub.1110370527
132 rdf:type schema:PropertyValue
133 N93f86f7aa9db47ac8fdb27425573ee47 schema:name doi
134 schema:value 10.1186/s40409-018-0172-9
135 rdf:type schema:PropertyValue
136 Nb25d20caf86c4adfb32a725bf21c958b schema:issueNumber 1
137 rdf:type schema:PublicationIssue
138 Neb5fbb2ff1ce4b468b7f22e759360f80 rdf:first sg:person.0657321564.92
139 rdf:rest N536f444b8657463d89d3e8f8e2099203
140 Nfc146325eda34c029c1d0c960d6616e3 rdf:first sg:person.07372115121.10
141 rdf:rest N1b91151cb4db44bea9f70040d203502a
142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biological Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
146 schema:name Biochemistry and Cell Biology
147 rdf:type schema:DefinedTerm
148 sg:grant.4476820 http://pending.schema.org/fundedItem sg:pub.10.1186/s40409-018-0172-9
149 rdf:type schema:MonetaryGrant
150 sg:grant.9106255 http://pending.schema.org/fundedItem sg:pub.10.1186/s40409-018-0172-9
151 rdf:type schema:MonetaryGrant
152 sg:journal.1357529 schema:issn 1678-9180
153 1678-9199
154 schema:name Journal of Venomous Animals and Toxins including Tropical Diseases
155 schema:publisher Springer Nature
156 rdf:type schema:Periodical
157 sg:person.01026117775.10 schema:affiliation grid-institutes:grid.11899.38
158 schema:familyName Menaldo
159 schema:givenName Danilo L.
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026117775.10
161 rdf:type schema:Person
162 sg:person.01051030743.43 schema:affiliation grid-institutes:grid.11899.38
163 schema:familyName Costa
164 schema:givenName Tássia R.
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051030743.43
166 rdf:type schema:Person
167 sg:person.014011333227.20 schema:affiliation grid-institutes:grid.11899.38
168 schema:familyName Tucci
169 schema:givenName Luiz F. F.
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014011333227.20
171 rdf:type schema:Person
172 sg:person.015352136017.70 schema:affiliation grid-institutes:grid.11899.38
173 schema:familyName Rosa-Garzon
174 schema:givenName Nathalia G.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015352136017.70
176 rdf:type schema:Person
177 sg:person.0657321564.92 schema:affiliation grid-institutes:grid.11899.38
178 schema:familyName Cabral
179 schema:givenName Hamilton
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657321564.92
181 rdf:type schema:Person
182 sg:person.07372115121.10 schema:affiliation grid-institutes:grid.11899.38
183 schema:familyName Carone
184 schema:givenName Sante E. I.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07372115121.10
186 rdf:type schema:Person
187 sg:person.0746137414.79 schema:affiliation grid-institutes:grid.11899.38
188 schema:familyName Sampaio
189 schema:givenName Suely V.
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746137414.79
191 rdf:type schema:Person
192 sg:pub.10.1007/s10495-006-7959-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047343183
193 https://doi.org/10.1007/s10495-006-7959-9
194 rdf:type schema:CreativeWork
195 sg:pub.10.1186/1678-9199-20-23 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000602197
196 https://doi.org/10.1186/1678-9199-20-23
197 rdf:type schema:CreativeWork
198 sg:pub.10.1186/s40409-015-0025-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043961926
199 https://doi.org/10.1186/s40409-015-0025-8
200 rdf:type schema:CreativeWork
201 sg:pub.10.1186/s40409-015-0026-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036525575
202 https://doi.org/10.1186/s40409-015-0026-7
203 rdf:type schema:CreativeWork
204 grid-institutes:grid.11899.38 schema:alternateName Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Avenida do Café, s/n, B. Monte Alegre, CEP 14040-903, Ribeirão Preto, SP, Brazil
205 Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Ribeirão Preto, SP, Brazil
206 schema:name Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Avenida do Café, s/n, B. Monte Alegre, CEP 14040-903, Ribeirão Preto, SP, Brazil
207 Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Ribeirão Preto, SP, Brazil
208 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...