Impact of autologous whole blood administration upon experimental mouse models of acute Trypanosoma cruzi infection View Full Text


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Article Info

DATE

2018-08-30

AUTHORS

Beatriz Philot Pavão, Kelly Cristina Demarque, Marcos Meuser Batista, Gabriel Melo de Oliveira, Cristiane França da Silva, Francisca Hildemagna Guedes da Silva, Luzia Fátima Gonçalves Caputo, Cynthia Machado Cascabulho, Marcello André Barcinski, Maria de Nazaré Correia Soeiro

ABSTRACT

BackgroundAutologous whole blood (AWB) administration is described as alternative/complementary medical practice widely employed in medical and veterinary therapy against infections, chronic pathologies and neoplasias. Our aim is to investigate in vivo biological effect of AWB using healthy murine models under the course of Trypanosoma cruzi acute infection.MethodsThe first set of studies consisted of injecting different volumes of AWB and saline (SAL) into the posterior region of quadriceps muscle of healthy male Swiss mice under distinct therapeutic schemes evaluating: animal behavior, body and organ weight, hemogram, plasmatic biochemical markers for tissue damage and inflammatory cytokine levels and profile. To assess the impact on the experimental T. cruzi infection, different schemes (prior and post infection) and periods of AWB administration (from one up to 10 days) were conducted, also employing heterologous whole blood (HWB) and evaluating plasma cytokine profile.ResultsNo major adverse events were observed in healthy AWB-treated mice, except gait impairment in animals that received three doses of 20 μL AWB in the same hind limb. AWB and SAL triggered an immediate polymorphonuclear response followed by mononuclear infiltrate. Although SAL triggered an inflammatory response, the kinetics and intensity of the histological profile and humoral mediator levels were different from AWB, the latter occurring earlier and more intensely with concomitant elevation of plasma IL-6. Inflammatory peak response of SAL, mainly composed of mononuclear cells with IL-10, was increased at 24 h. According to the mouse model of acute T. cruzi infection, only minor decreases (< 30%) in the parasitemia levels were produced by AWB and HWB given before and after infection, without protecting against mortality. Rises in IFN-gamma, TNF-alpha and IL-6 were detected at 9 dpi in all infected animals as compared to uninfected mice but only Bz displayed a statistically significant diminution (p = 0.02) in TNF-alpha levels than infected and untreated mice.ConclusionsThis study revealed that the use of autologous whole blood (AWB) in the acute model employed was unable to reduce the parasitic load of infected mice, providing only a minor decrease in parasitemia levels (up to 30%) but without protecting against animal mortality. Further in vivo studies will be necessary to elucidate the effective impact of this procedure. More... »

PAGES

25

References to SciGraph publications

  • 2006-03-22. Ultrasound-guided autologous blood injection for tennis elbow in SKELETAL RADIOLOGY
  • 2010-02-15. The regulation of IL-10 production by immune cells in NATURE REVIEWS IMMUNOLOGY
  • 2013-12-19. Challenges and perspectives of Chagas disease: a review in JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES
  • 2013-10-07. Repeated autologous intraarticular blood injections as an animal model for joint pain in haemophilic arthropathy in ARTHRITIS RESEARCH & THERAPY
  • 2017-12-12. Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection in JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES
  • 1982-02. Interaction ofTrypanosoma cruzi with macrophages in vitro: Dissociation of the attachment and internalization phases by low temperature and cytochalasin B in ZEITSCHRIFT FÜR PARASITENKUNDE PARASITOLOGY RESEARCH
  • 2016-02-17. The use of ayurvedic medicine in the context of health promotion – a mixed methods case study of an ayurvedic centre in Sweden in BMC COMPLEMENTARY MEDICINE AND THERAPIES
  • 2015-12. Complementary alternative medicine use among patients with dengue fever in the hospital setting: a cross-sectional study in Malaysia in BMC COMPLEMENTARY MEDICINE AND THERAPIES
  • 2008-02-22. Adult vaccination coverage levels among users of complementary/alternative medicine – results from the 2002 National Health Interview Survey (NHIS) in BMC COMPLEMENTARY MEDICINE AND THERAPIES
  • 2016-01-15. Hypnosis can reduce pain in hospitalized older patients: a randomized controlled study in BMC GERIATRICS
  • 2015-04-21. IL-6 as a keystone cytokine in health and disease in NATURE IMMUNOLOGY
  • 2011-11-14. Ozonated autohemotherapy: protection of kidneys from ischemia in rats subjected to unilateral nephrectomy in BMC NEPHROLOGY
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    http://scigraph.springernature.com/pub.10.1186/s40409-018-0157-8

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    http://dx.doi.org/10.1186/s40409-018-0157-8

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30186314


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    18 schema:description BackgroundAutologous whole blood (AWB) administration is described as alternative/complementary medical practice widely employed in medical and veterinary therapy against infections, chronic pathologies and neoplasias. Our aim is to investigate in vivo biological effect of AWB using healthy murine models under the course of Trypanosoma cruzi acute infection.MethodsThe first set of studies consisted of injecting different volumes of AWB and saline (SAL) into the posterior region of quadriceps muscle of healthy male Swiss mice under distinct therapeutic schemes evaluating: animal behavior, body and organ weight, hemogram, plasmatic biochemical markers for tissue damage and inflammatory cytokine levels and profile. To assess the impact on the experimental T. cruzi infection, different schemes (prior and post infection) and periods of AWB administration (from one up to 10 days) were conducted, also employing heterologous whole blood (HWB) and evaluating plasma cytokine profile.ResultsNo major adverse events were observed in healthy AWB-treated mice, except gait impairment in animals that received three doses of 20 μL AWB in the same hind limb. AWB and SAL triggered an immediate polymorphonuclear response followed by mononuclear infiltrate. Although SAL triggered an inflammatory response, the kinetics and intensity of the histological profile and humoral mediator levels were different from AWB, the latter occurring earlier and more intensely with concomitant elevation of plasma IL-6. Inflammatory peak response of SAL, mainly composed of mononuclear cells with IL-10, was increased at 24 h. According to the mouse model of acute T. cruzi infection, only minor decreases (< 30%) in the parasitemia levels were produced by AWB and HWB given before and after infection, without protecting against mortality. Rises in IFN-gamma, TNF-alpha and IL-6 were detected at 9 dpi in all infected animals as compared to uninfected mice but only Bz displayed a statistically significant diminution (p = 0.02) in TNF-alpha levels than infected and untreated mice.ConclusionsThis study revealed that the use of autologous whole blood (AWB) in the acute model employed was unable to reduce the parasitic load of infected mice, providing only a minor decrease in parasitemia levels (up to 30%) but without protecting against animal mortality. Further in vivo studies will be necessary to elucidate the effective impact of this procedure.
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    25 ConclusionsThis study
    26 IFN-gamma
    27 IL-10
    28 IL-6
    29 Swiss mice
    30 TNF-alpha
    31 TNF-alpha levels
    32 Trypanosoma
    33 Trypanosoma cruzi infection
    34 acute Trypanosoma cruzi infection
    35 acute infection
    36 acute model
    37 administration
    38 adverse events
    39 aim
    40 animal behavior
    41 animal mortality
    42 animals
    43 autologous whole blood
    44 behavior
    45 biochemical markers
    46 biological effects
    47 blood
    48 blood administration
    49 body
    50 cells
    51 chronic pathologies
    52 complementary medical practices
    53 concomitant elevation
    54 course
    55 cruzi infection
    56 cytokine levels
    57 cytokine profile
    58 damage
    59 decrease
    60 different schemes
    61 different volumes
    62 diminution
    63 doses
    64 dpi
    65 effect
    66 effective impact
    67 elevation
    68 events
    69 experimental mouse model
    70 first set
    71 gait impairment
    72 healthy murine models
    73 hemogram
    74 hind limb
    75 histological profile
    76 impact
    77 impairment
    78 infected mice
    79 infection
    80 infiltrates
    81 inflammatory cytokine levels
    82 inflammatory response
    83 intensity
    84 kinetics
    85 levels
    86 limb
    87 load
    88 major adverse events
    89 male Swiss mice
    90 markers
    91 mediator levels
    92 medical practice
    93 mice
    94 minor decrease
    95 model
    96 mononuclear cells
    97 mononuclear infiltrate
    98 mortality
    99 mouse model
    100 murine model
    101 muscle
    102 neoplasia
    103 only minor decreases
    104 organ weights
    105 parasitemia levels
    106 parasitic load
    107 pathology
    108 peak response
    109 period
    110 plasma IL-6
    111 plasma cytokine profiles
    112 polymorphonuclear response
    113 posterior region
    114 practice
    115 procedure
    116 profile
    117 quadriceps muscle
    118 region
    119 response
    120 rise
    121 saline
    122 scheme
    123 set
    124 significant diminution
    125 study
    126 therapeutic schemes
    127 therapy
    128 tissue damage
    129 uninfected mice
    130 untreated mice
    131 use
    132 veterinary therapy
    133 vivo biological effects
    134 vivo studies
    135 volume
    136 weight
    137 whole blood
    138 whole blood administration
    139 schema:name Impact of autologous whole blood administration upon experimental mouse models of acute Trypanosoma cruzi infection
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