Dietary Bacillus spp. enhanced growth and disease resistance of weaned pigs by modulating intestinal microbiota and systemic immunity View Full Text


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Article Info

DATE

2020-09-15

AUTHORS

Yijie He, Cynthia Jinno, Kwangwook Kim, Zhaohai Wu, Bie Tan, Xunde Li, Rose Whelan, Yanhong Liu

ABSTRACT

BackgroundPrevious research has shown that dietary supplementation of Bacillus spp. probiotics exerts beneficial effects on animals’ growth. However, limited studies have evaluated the efficacy of Bacillus spp. on weaned pigs and their effects on host gut health and microbiome, and systemic immunity using a disease challenge model. The objective of this experiment was to investigate the effects of two Bacillus spp. strains (Bacillus subtilis DSM 32540 and Bacillus pumilus DSM 32539) on growth performance, diarrhea, intestinal health, microbiome, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli (ETEC).ResultsPigs in PRO1 (Bacillus subtilis DSM 32540) had greater (P < 0.05) body weight on d 7 and 14 PI, greater (P < 0.05) ADG from d 0 to 7 and d 7 to 14 PI, compared with pigs in CON (Control). Pigs in PRO1 had milder (P < 0.05) diarrhea on d 2 and 3 PI compared with pigs in CON. However, no differences were observed in growth performance and diarrhea score between PRO2 (Bacillus pumilus DSM 32539) and CON groups. Supplementation of PRO1 decreased (P < 0.05) lymphocyte counts on d 7 and 14 PI, compared with CON. Supplementation of PRO1 and PRO2 both reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO2 had greater (P < 0.05) goblet cell number and sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in CON. Supplementation of PRO1 up-regulated (P < 0.05) MUC2 gene expression in jejunal mucosa and reduced (P < 0.05) PTGS-2 and IL1B gene expression in ileal mucosa on d 21 PI, compared with CON. Pigs in PRO1 had reduced (P < 0.05) relative abundance of families Lachnospiraceae, Peptostreptococcaceae and Pasteurellaceae in the ileum.ConclusionsSupplementation of Bacillus subtilis DSM 32540 improved growth performance, alleviated diarrhea severity, enhanced gut health, and reduced systemic inflammation of weaned pigs infected with ETEC F18. Although Bacillus pumilus DSM 32539 was able to alleviate systemic inflammation, it had limited impacts on growth performance and severity of diarrhea of ETEC F18 challenged weaned pigs. More... »

PAGES

101

References to SciGraph publications

  • 2017-03-02. Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model in SCIENTIFIC REPORTS
  • 2010-08-04. Intestinal Stem Cells in CURRENT GASTROENTEROLOGY REPORTS
  • 2012-03-08. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2019-09-12. Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics in JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
  • 2019-06-25. Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli in JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
  • 2014-02-13. Bacteria, phages and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2015-05-20. Stability of operational taxonomic units: an important but neglected property for analyzing microbial diversity in MICROBIOME
  • 2018-10-18. Supplemental Bacillus subtilis DSM 32315 manipulates intestinal structure and microbial composition in broiler chickens in SCIENTIFIC REPORTS
  • 2018-05-17. Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin in MICROBIOME
  • 2016-09-01. Prevalence of virulence factors in enterotoxigenic Escherichia coli isolated from pigs with post-weaning diarrhoea in Europe in PORCINE HEALTH MANAGEMENT
  • 2017-05-19. Post weaning diarrhea in pigs: risk factors and non-colistin-based control strategies in ACTA VETERINARIA SCANDINAVICA
  • 2018-08-24. Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions in SCIENTIFIC REPORTS
  • 2018-07-30. The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition in JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
  • 2010-04-11. QIIME allows analysis of high-throughput community sequencing data in NATURE METHODS
  • 2017-05-23. Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality in JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
  • 2002-01. Platelet-activating factor receptor and ADAM10 mediate responses to Staphylococcus aureus in epithelial cells in NATURE MEDICINE
  • 2016-05-23. DADA2: High-resolution sample inference from Illumina amplicon data in NATURE METHODS
  • 2006-07-04. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs in ANTONIE VAN LEEUWENHOEK
  • 2018-03-14. A global comparison of the microbiome compositions of three gut locations in commercial pigs with extreme feed conversion ratios in SCIENTIFIC REPORTS
  • 2018-06-06. The role of probiotics, prebiotics and synbiotics in animal nutrition in GUT PATHOGENS
  • 2019-07-24. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 in NATURE BIOTECHNOLOGY
  • 2013-12-20. Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs in JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s40104-020-00498-3

    DOI

    http://dx.doi.org/10.1186/s40104-020-00498-3

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32944236


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    28 schema:description BackgroundPrevious research has shown that dietary supplementation of Bacillus spp. probiotics exerts beneficial effects on animals’ growth. However, limited studies have evaluated the efficacy of Bacillus spp. on weaned pigs and their effects on host gut health and microbiome, and systemic immunity using a disease challenge model. The objective of this experiment was to investigate the effects of two Bacillus spp. strains (Bacillus subtilis DSM 32540 and Bacillus pumilus DSM 32539) on growth performance, diarrhea, intestinal health, microbiome, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli (ETEC).ResultsPigs in PRO1 (Bacillus subtilis DSM 32540) had greater (P < 0.05) body weight on d 7 and 14 PI, greater (P < 0.05) ADG from d 0 to 7 and d 7 to 14 PI, compared with pigs in CON (Control). Pigs in PRO1 had milder (P < 0.05) diarrhea on d 2 and 3 PI compared with pigs in CON. However, no differences were observed in growth performance and diarrhea score between PRO2 (Bacillus pumilus DSM 32539) and CON groups. Supplementation of PRO1 decreased (P < 0.05) lymphocyte counts on d 7 and 14 PI, compared with CON. Supplementation of PRO1 and PRO2 both reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO2 had greater (P < 0.05) goblet cell number and sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in CON. Supplementation of PRO1 up-regulated (P < 0.05) MUC2 gene expression in jejunal mucosa and reduced (P < 0.05) PTGS-2 and IL1B gene expression in ileal mucosa on d 21 PI, compared with CON. Pigs in PRO1 had reduced (P < 0.05) relative abundance of families Lachnospiraceae, Peptostreptococcaceae and Pasteurellaceae in the ileum.ConclusionsSupplementation of Bacillus subtilis DSM 32540 improved growth performance, alleviated diarrhea severity, enhanced gut health, and reduced systemic inflammation of weaned pigs infected with ETEC F18. Although Bacillus pumilus DSM 32539 was able to alleviate systemic inflammation, it had limited impacts on growth performance and severity of diarrhea of ETEC F18 challenged weaned pigs.
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