Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Liang Wu, Xiaolong Zhang, Zhikun Zhao, Ling Wang, Bo Li, Guibo Li, Michael Dean, Qichao Yu, Yanhui Wang, Xinxin Lin, Weijian Rao, Zhanlong Mei, Yang Li, Runze Jiang, Huan Yang, Fuqiang Li, Guoyun Xie, Liqin Xu, Kui Wu, Jie Zhang, Jianghao Chen, Ting Wang, Karsten Kristiansen, Xiuqing Zhang, Yingrui Li, Huanming Yang, Jian Wang, Yong Hou, Xun Xu

ABSTRACT

BACKGROUND: Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line. RESULT: We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins. CONCLUSION: Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers. More... »

PAGES

51

References to SciGraph publications

  • 2001-02. Initial sequencing and analysis of the human genome in NATURE
  • 2013-08. The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line in NATURE
  • 2014-05. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus in CELL RESEARCH
  • 2014-12. C-Myc-activated long noncoding RNA CCAT1 promotes colon cancer cell proliferation and invasion in TUMOR BIOLOGY
  • 2009-03. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome in GENOME BIOLOGY
  • 2011-12. The HPV-16 E7 oncoprotein induces centriole multiplication through deregulation of Polo-like kinase 4 expression in MOLECULAR CANCER
  • 2008-12. WGCNA: an R package for weighted correlation network analysis in BMC BIOINFORMATICS
  • 2001-02. Regulation of the Cdc25A gene by the human papillomavirus Type 16 E7 oncogene in ONCOGENE
  • 2010-12. Why do viruses cause cancer? Highlights of the first century of human tumour virology in NATURE REVIEWS CANCER
  • 2011-01. Integrative genomics viewer in NATURE BIOTECHNOLOGY
  • 2014-01. Full-length RNA-seq from single cells using Smart-seq2 in NATURE PROTOCOLS
  • 2015-02. Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism in NATURE GENETICS
  • 2012-04. Fast gapped-read alignment with Bowtie 2 in NATURE METHODS
  • 2013-06. Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells in NATURE
  • 2011-04. Tumour evolution inferred by single-cell sequencing in NATURE
  • 2011-08. TopHat-Fusion: an algorithm for discovery of novel fusion transcripts in GENOME BIOLOGY
  • 1999-12. HPV-18 E6*I protein modulates the E6-directed degradation of p53 by binding to full-length HPV-18 E6 in ONCOGENE
  • 2007-04. The impact of translocations and gene fusions on cancer causation in NATURE REVIEWS CANCER
  • 2010-10-28. Distant metastasis occurs late during the genetic evolution of pancreatic cancer in NATURE
  • 2015-06. Single-cell gene expression signatures reveal melanoma cell heterogeneity in ONCOGENE
  • 2012-03-01. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks in NATURE PROTOCOLS
  • 2011-09. Stem cell gene expression programs influence clinical outcome in human leukemia in NATURE MEDICINE
  • 2011-01. Identification of fusion genes in breast cancer by paired-end RNA-sequencing in GENOME BIOLOGY
  • 2012-12. Human tumour viruses and the deregulation of cell polarity in cancer in NATURE REVIEWS CANCER
  • 2014-02. Quantitative single-cell RNA-seq with unique molecular identifiers in NATURE METHODS
  • 2015-03. Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells in NATURE BIOTECHNOLOGY
  • 2013-12. Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells in NATURE BIOTECHNOLOGY
  • 2007-03. Reactome: a knowledge base of biologic pathways and processes in GENOME BIOLOGY
  • 2013-02. Pathogenic mechanisms in HBV- and HCV-associated hepatocellular carcinoma in NATURE REVIEWS CANCER
  • 2013-12. The landscape of viral expression and host gene fusion and adaptation in human cancer in NATURE COMMUNICATIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4

    DOI

    http://dx.doi.org/10.1186/s13742-015-0091-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020976552

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26550473


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alphapapillomavirus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Heterogeneity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HeLa Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA Splicing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, RNA", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Liang", 
            "id": "sg:person.011026731327.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011026731327.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "College of Life Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xiaolong", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Southeast University", 
              "id": "https://www.grid.ac/institutes/grid.263826.b", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "State Key Laboratory of Bioelectronics, Southeast University, 210096, Nanjing, China", 
                "School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhao", 
            "givenName": "Zhikun", 
            "id": "sg:person.01162141537.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162141537.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi\u2019an, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Ling", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Bo", 
            "id": "sg:person.01303526064.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303526064.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Copenhagen", 
              "id": "https://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Guibo", 
            "id": "sg:person.013612667131.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013612667131.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Cancer Institute", 
              "id": "https://www.grid.ac/institutes/grid.48336.3a", 
              "name": [
                "Cancer and Inflammation Program, National Cancer Institute at Frederick, Building 560, 21702, Frederick, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dean", 
            "givenName": "Michael", 
            "id": "sg:person.01031604372.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031604372.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "BGI-Education Center, University of Chinese Academy of Sciences, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Qichao", 
            "id": "sg:person.0667212437.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667212437.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Yanhui", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lin", 
            "givenName": "Xinxin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rao", 
            "givenName": "Weijian", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mei", 
            "givenName": "Zhanlong", 
            "id": "sg:person.01371136134.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371136134.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Yang", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jiang", 
            "givenName": "Runze", 
            "id": "sg:person.0707574237.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707574237.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Huan", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Fuqiang", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xie", 
            "givenName": "Guoyun", 
            "id": "sg:person.01254477637.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254477637.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Liqin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Kui", 
            "id": "sg:person.0603065207.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603065207.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Jie", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi\u2019an, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Jianghao", 
            "id": "sg:person.01353167646.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353167646.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi\u2019an, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Ting", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Copenhagen", 
              "id": "https://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kristiansen", 
            "givenName": "Karsten", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "The Guangdong Enterprise Key Laboratory of Human Disease Genomics, BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xiuqing", 
            "id": "sg:person.0762053777.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762053777.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Queensland", 
              "id": "https://www.grid.ac/institutes/grid.1003.2", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Yingrui", 
            "id": "sg:person.01372000452.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372000452.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Zhejiang University", 
              "id": "https://www.grid.ac/institutes/grid.13402.34", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Huanming", 
            "id": "sg:person.01231641167.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231641167.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Zhejiang University", 
              "id": "https://www.grid.ac/institutes/grid.13402.34", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Jian", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Copenhagen", 
              "id": "https://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hou", 
            "givenName": "Yong", 
            "id": "sg:person.01100032432.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100032432.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Xun", 
            "id": "sg:person.01143540012.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143540012.53"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1093/nar/gkg786", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000364966"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.3178", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000968113", 
              "https://doi.org/10.1038/ng.3178"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ccr.2014.01.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002498840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1476-4598-10-61", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006038094", 
              "https://doi.org/10.1186/1476-4598-10-61"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2011-12-8-r72", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006381787", 
              "https://doi.org/10.1186/gb-2011-12-8-r72"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1923", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006541515", 
              "https://doi.org/10.1038/nmeth.1923"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1470-2045(09)70096-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009050725"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tig.2012.07.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009222806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/onc.2014.262", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009744407", 
              "https://doi.org/10.1038/onc.2014.262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.135350.111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012073457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-05-2573", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012224313"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ol.2013.1483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012344741"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ol.2013.1483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012344741"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1136/jclinpath-2014-202560", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013869709"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.chom.2014.02.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014449031"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt1196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015989191"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.3102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016103324", 
              "https://doi.org/10.1038/nbt.3102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1203134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016305716", 
              "https://doi.org/10.1038/sj.onc.1203134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1203134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016305716", 
              "https://doi.org/10.1038/sj.onc.1203134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejm199601113340213", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016487843"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2014.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017950805", 
              "https://doi.org/10.1038/nprot.2014.006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.161034.113", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018538260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1754", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019307928", 
              "https://doi.org/10.1038/nbt.1754"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.02-02-0030.", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020036628"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virol.2013.12.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020109637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2105-9-559", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020312314", 
              "https://doi.org/10.1186/1471-2105-9-559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09807", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020406938", 
              "https://doi.org/10.1038/nature09807"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0116151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020563858"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-06-0571", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020795903"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2015.03.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022270616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pcbi.1003696", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022849102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp352", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023014918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp616", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023247882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s13277-014-2526-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023363208", 
              "https://doi.org/10.1007/s13277-014-2526-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc3400", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024896356", 
              "https://doi.org/10.1038/nrc3400"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.11.16.2090", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026178926"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.chom.2014.02.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026644575"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.01162-09", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027858553"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/g3.113.005777", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028964445"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/g3.113.005777", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028964445"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09515", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029325111", 
              "https://doi.org/10.1038/nature09515"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09515", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029325111", 
              "https://doi.org/10.1038/nature09515"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2772", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029706604", 
              "https://doi.org/10.1038/nmeth.2772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2012.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030124536", 
              "https://doi.org/10.1038/nprot.2012.016"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2014.35", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032435968", 
              "https://doi.org/10.1038/cr.2014.35"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.24844", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034369046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.24844", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034369046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12172", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035006736", 
              "https://doi.org/10.1038/nature12172"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejmoa021641", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036284978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms3513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036430565", 
              "https://doi.org/10.1038/ncomms3513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1204130", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036918842", 
              "https://doi.org/10.1038/sj.onc.1204130"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1204130", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036918842", 
              "https://doi.org/10.1038/sj.onc.1204130"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1470-2045(12)70137-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037022536"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jhep.2006.05.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037562150"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0304-3835(01)00871-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037780457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.02-02-0030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038504953"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/bts678", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038581758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gku1207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039030168"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0016266", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039493284"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc3449", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039804755", 
              "https://doi.org/10.1038/nrc3449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(90)90409-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040120250"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12064", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040190826", 
              "https://doi.org/10.1038/nature12064"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-14-0122", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041393209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virol.2009.06.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041496962"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2014.08.029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041908806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc2961", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042558338", 
              "https://doi.org/10.1038/nrc2961"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc2961", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042558338", 
              "https://doi.org/10.1038/nrc2961"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35057062", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042854081", 
              "https://doi.org/10.1038/35057062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35057062", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042854081", 
              "https://doi.org/10.1038/35057062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc2091", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043783272", 
              "https://doi.org/10.1038/nrc2091"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.canlet.2011.02.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043991108"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045584197", 
              "https://doi.org/10.1038/nbt.2720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1254257", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046815039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2009-10-3-r25", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049583368", 
              "https://doi.org/10.1186/gb-2009-10-3-r25"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ejogrb.2009.07.012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049915789"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pcbi.1002464", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050428866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2011-12-1-r6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050465130", 
              "https://doi.org/10.1186/gb-2011-12-1-r6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.2415", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051273515", 
              "https://doi.org/10.1038/nm.2415"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2007-8-3-r39", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051807491", 
              "https://doi.org/10.1186/gb-2007-8-3-r39"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1156725", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062457660"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1289/ehp.95103s8259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064746153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/or_00000729", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071537513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074493152", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075179519", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075570876", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079008116", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082847169", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2015-12", 
        "datePublishedReg": "2015-12-01", 
        "description": "BACKGROUND: Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line.\nRESULT: We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins.\nCONCLUSION: Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s13742-015-0091-4", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.7015769", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.7200984", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1047731", 
            "issn": [
              "2047-217X"
            ], 
            "name": "GigaScience", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "4"
          }
        ], 
        "name": "Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells", 
        "pagination": "51", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "63f19f1897e987425f951bfbac94bb26c82c3e35214d3f39676ca24848a101fc"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "26550473"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101596872"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s13742-015-0091-4"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1020976552"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s13742-015-0091-4", 
          "https://app.dimensions.ai/details/publication/pub.1020976552"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T01:09", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000521.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1186%2Fs13742-015-0091-4"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'


     

    This table displays all metadata directly associated to this object as RDF triples.

    583 TRIPLES      21 PREDICATES      117 URIs      28 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s13742-015-0091-4 schema:about N46185e27577b4e18bda1c36d681718ad
    2 Nc51bc86c9b9847c2a7310ced1d7092f4
    3 Nc5ba11e5c11c413e92e9d28b29c25d07
    4 Nc7f8c99112944bc7850d8e65da2d1e81
    5 Nd0b21a891af3487a889d8f3d2f9d0353
    6 Ndd6d239b507149ff9875356c4a3c8741
    7 Nef4f9653c90c4113a1050d4214b5c14f
    8 anzsrc-for:06
    9 anzsrc-for:0604
    10 schema:author N89c1745ecfea43879f0129fff32045d0
    11 schema:citation sg:pub.10.1007/s13277-014-2526-4
    12 sg:pub.10.1038/35057062
    13 sg:pub.10.1038/cr.2014.35
    14 sg:pub.10.1038/nature09515
    15 sg:pub.10.1038/nature09807
    16 sg:pub.10.1038/nature12064
    17 sg:pub.10.1038/nature12172
    18 sg:pub.10.1038/nbt.1754
    19 sg:pub.10.1038/nbt.2720
    20 sg:pub.10.1038/nbt.3102
    21 sg:pub.10.1038/ncomms3513
    22 sg:pub.10.1038/ng.3178
    23 sg:pub.10.1038/nm.2415
    24 sg:pub.10.1038/nmeth.1923
    25 sg:pub.10.1038/nmeth.2772
    26 sg:pub.10.1038/nprot.2012.016
    27 sg:pub.10.1038/nprot.2014.006
    28 sg:pub.10.1038/nrc2091
    29 sg:pub.10.1038/nrc2961
    30 sg:pub.10.1038/nrc3400
    31 sg:pub.10.1038/nrc3449
    32 sg:pub.10.1038/onc.2014.262
    33 sg:pub.10.1038/sj.onc.1203134
    34 sg:pub.10.1038/sj.onc.1204130
    35 sg:pub.10.1186/1471-2105-9-559
    36 sg:pub.10.1186/1476-4598-10-61
    37 sg:pub.10.1186/gb-2007-8-3-r39
    38 sg:pub.10.1186/gb-2009-10-3-r25
    39 sg:pub.10.1186/gb-2011-12-1-r6
    40 sg:pub.10.1186/gb-2011-12-8-r72
    41 https://app.dimensions.ai/details/publication/pub.1074493152
    42 https://app.dimensions.ai/details/publication/pub.1075179519
    43 https://app.dimensions.ai/details/publication/pub.1075570876
    44 https://app.dimensions.ai/details/publication/pub.1079008116
    45 https://app.dimensions.ai/details/publication/pub.1082847169
    46 https://doi.org/10.1002/ijc.24844
    47 https://doi.org/10.1016/0092-8674(90)90409-8
    48 https://doi.org/10.1016/j.canlet.2011.02.007
    49 https://doi.org/10.1016/j.ccr.2014.01.030
    50 https://doi.org/10.1016/j.celrep.2014.08.029
    51 https://doi.org/10.1016/j.chom.2014.02.010
    52 https://doi.org/10.1016/j.chom.2014.02.011
    53 https://doi.org/10.1016/j.ejogrb.2009.07.012
    54 https://doi.org/10.1016/j.jhep.2006.05.013
    55 https://doi.org/10.1016/j.molcel.2015.03.005
    56 https://doi.org/10.1016/j.tig.2012.07.003
    57 https://doi.org/10.1016/j.virol.2009.06.001
    58 https://doi.org/10.1016/j.virol.2013.12.011
    59 https://doi.org/10.1016/s0304-3835(01)00871-0
    60 https://doi.org/10.1016/s1470-2045(09)70096-8
    61 https://doi.org/10.1016/s1470-2045(12)70137-7
    62 https://doi.org/10.1056/nejm199601113340213
    63 https://doi.org/10.1056/nejmoa021641
    64 https://doi.org/10.1073/pnas.0506580102
    65 https://doi.org/10.1091/mbc.02-02-0030
    66 https://doi.org/10.1091/mbc.02-02-0030.
    67 https://doi.org/10.1093/bioinformatics/btp352
    68 https://doi.org/10.1093/bioinformatics/btp616
    69 https://doi.org/10.1093/bioinformatics/bts678
    70 https://doi.org/10.1093/nar/gkg786
    71 https://doi.org/10.1093/nar/gkt1196
    72 https://doi.org/10.1093/nar/gku1207
    73 https://doi.org/10.1101/gad.11.16.2090
    74 https://doi.org/10.1101/gr.135350.111
    75 https://doi.org/10.1101/gr.161034.113
    76 https://doi.org/10.1111/igc.0b013e3181af711e
    77 https://doi.org/10.1126/science.1156725
    78 https://doi.org/10.1126/science.1254257
    79 https://doi.org/10.1128/jvi.01162-09
    80 https://doi.org/10.1136/jclinpath-2014-202560
    81 https://doi.org/10.1158/1078-0432.ccr-05-2573
    82 https://doi.org/10.1158/1078-0432.ccr-06-0571
    83 https://doi.org/10.1158/1078-0432.ccr-14-0122
    84 https://doi.org/10.1289/ehp.95103s8259
    85 https://doi.org/10.1371/journal.pcbi.1002464
    86 https://doi.org/10.1371/journal.pcbi.1003696
    87 https://doi.org/10.1371/journal.pone.0016266
    88 https://doi.org/10.1371/journal.pone.0116151
    89 https://doi.org/10.1534/g3.113.005777
    90 https://doi.org/10.3892/ol.2013.1483
    91 https://doi.org/10.3892/or_00000729
    92 schema:datePublished 2015-12
    93 schema:datePublishedReg 2015-12-01
    94 schema:description BACKGROUND: Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line. RESULT: We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins. CONCLUSION: Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers.
    95 schema:genre research_article
    96 schema:inLanguage en
    97 schema:isAccessibleForFree true
    98 schema:isPartOf N3bec4e8ef55b4a5390aaab57d29a380d
    99 N6a9cd0ff2d2f4ffda101f16d8a24ef9c
    100 sg:journal.1047731
    101 schema:name Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells
    102 schema:pagination 51
    103 schema:productId N317fe74a5e244ab9a8322b3626ee8553
    104 N6e0141e0b129475a998418a2b6c89dd9
    105 N8ffb57dd00e34b82a5433292fab46d04
    106 Nbf470abc97634fe4855f4c76e625a1bf
    107 Nc2ab56b565ef48979ba7d3d1bb58c39a
    108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020976552
    109 https://doi.org/10.1186/s13742-015-0091-4
    110 schema:sdDatePublished 2019-04-11T01:09
    111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    112 schema:sdPublisher Nfc8c5ea640084a349a9907a14714725d
    113 schema:url http://link.springer.com/10.1186%2Fs13742-015-0091-4
    114 sgo:license sg:explorer/license/
    115 sgo:sdDataset articles
    116 rdf:type schema:ScholarlyArticle
    117 N07ebc949fc854ae69c1b795a253884ce rdf:first N64f7aed6a4194175be8edfac282aa8e4
    118 rdf:rest N5a9bf82525a04b7286b3a8b9529c34f1
    119 N190c6fde441d42fd87f77b68edc09bc7 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    120 schema:familyName Li
    121 schema:givenName Fuqiang
    122 rdf:type schema:Person
    123 N19a584f81e81499fad966f15d93e9a04 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
    124 schema:familyName Kristiansen
    125 schema:givenName Karsten
    126 rdf:type schema:Person
    127 N1dfb26f0d40f4223be2fff14f08882a4 rdf:first sg:person.01254477637.32
    128 rdf:rest Ne8bc988d47aa49349f74ee5507a37b1d
    129 N317fe74a5e244ab9a8322b3626ee8553 schema:name doi
    130 schema:value 10.1186/s13742-015-0091-4
    131 rdf:type schema:PropertyValue
    132 N34a2d2e93af945b38b1c6c5707e1b031 rdf:first Neb04f0e180ac4c90a82029336f20da72
    133 rdf:rest Nde256961f76746969a349e66e6af62c4
    134 N3bec4e8ef55b4a5390aaab57d29a380d schema:issueNumber 1
    135 rdf:type schema:PublicationIssue
    136 N3dd4d7b329e44f878a27b6ebb7ec2382 rdf:first sg:person.01143540012.53
    137 rdf:rest rdf:nil
    138 N432fdc18664d4bf2b86fd45597c18cea rdf:first N5b00c86579c14a0a90edf194df222926
    139 rdf:rest Nd25146cd576246e4a8e31d9ba3e0a84a
    140 N46185e27577b4e18bda1c36d681718ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Humans
    142 rdf:type schema:DefinedTerm
    143 N46c6d659593140b9b90af8b7f999993d rdf:first Nc8d64f98c71b4330a989d9addd4d386d
    144 rdf:rest Ncfec86d3b57b4ba49831b11bb07df38d
    145 N488aab4aaa394b04b18bd1c8433b2c15 rdf:first sg:person.01031604372.16
    146 rdf:rest N61db4da1191e4894a422b0a00764ee7d
    147 N4de17ae941f64ace9d7f365ef1489c93 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    148 schema:familyName Lin
    149 schema:givenName Xinxin
    150 rdf:type schema:Person
    151 N5a9bf82525a04b7286b3a8b9529c34f1 rdf:first N190c6fde441d42fd87f77b68edc09bc7
    152 rdf:rest N1dfb26f0d40f4223be2fff14f08882a4
    153 N5b00c86579c14a0a90edf194df222926 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    154 schema:familyName Wang
    155 schema:givenName Yanhui
    156 rdf:type schema:Person
    157 N61db4da1191e4894a422b0a00764ee7d rdf:first sg:person.0667212437.82
    158 rdf:rest N432fdc18664d4bf2b86fd45597c18cea
    159 N64f7aed6a4194175be8edfac282aa8e4 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    160 schema:familyName Yang
    161 schema:givenName Huan
    162 rdf:type schema:Person
    163 N67a14e7e35854267af453e0f9e7588b1 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    164 schema:familyName Xu
    165 schema:givenName Liqin
    166 rdf:type schema:Person
    167 N6a9cd0ff2d2f4ffda101f16d8a24ef9c schema:volumeNumber 4
    168 rdf:type schema:PublicationVolume
    169 N6e0141e0b129475a998418a2b6c89dd9 schema:name readcube_id
    170 schema:value 63f19f1897e987425f951bfbac94bb26c82c3e35214d3f39676ca24848a101fc
    171 rdf:type schema:PropertyValue
    172 N6e7e82b1e41c47b1a5914533bf9eab03 rdf:first sg:person.01231641167.20
    173 rdf:rest Nb49a53c186ed4da0a7fb46607adda28a
    174 N7956d5aac4cf4dd283ee128727a67c9e schema:name Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China
    175 rdf:type schema:Organization
    176 N836fafd572d246f5b1d8da18bdca5346 schema:affiliation https://www.grid.ac/institutes/grid.13402.34
    177 schema:familyName Wang
    178 schema:givenName Jian
    179 rdf:type schema:Person
    180 N89c1745ecfea43879f0129fff32045d0 rdf:first sg:person.011026731327.79
    181 rdf:rest Na63e999f35ed41ec878e9dcf0883e343
    182 N8cf654cdc69740d1a86cda6a1d2044c3 rdf:first N19a584f81e81499fad966f15d93e9a04
    183 rdf:rest Ned7b19e2b72842aeb2583b9740bcb54f
    184 N8ffb57dd00e34b82a5433292fab46d04 schema:name nlm_unique_id
    185 schema:value 101596872
    186 rdf:type schema:PropertyValue
    187 N9156d763ce654c66bf41640d7e1cbe51 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    188 schema:familyName Zhang
    189 schema:givenName Xiaolong
    190 rdf:type schema:Person
    191 N9db0c15443c7473aa29f26109a4f9ad4 schema:affiliation N7956d5aac4cf4dd283ee128727a67c9e
    192 schema:familyName Wang
    193 schema:givenName Ting
    194 rdf:type schema:Person
    195 Na558ca55cc3d46a4923b1540a5f73b11 rdf:first sg:person.0707574237.39
    196 rdf:rest N07ebc949fc854ae69c1b795a253884ce
    197 Na63e999f35ed41ec878e9dcf0883e343 rdf:first N9156d763ce654c66bf41640d7e1cbe51
    198 rdf:rest Nb89509b428d745209d7db17e27dd1e71
    199 Nab1f89b5f3f443429bb6cbcf392d8ccc schema:name Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China
    200 rdf:type schema:Organization
    201 Nb249ec0605f64f4b8f54dc7be333b867 rdf:first Ne3551195d83c47138a36f74abe271577
    202 rdf:rest Nbe041894c6a2429e9e01870238f4303a
    203 Nb49a53c186ed4da0a7fb46607adda28a rdf:first N836fafd572d246f5b1d8da18bdca5346
    204 rdf:rest Nb7bab2d86afd43abbb3f8cad09779682
    205 Nb6d0e38c3adf44e1b2690bd9b2b58bc6 rdf:first sg:person.0603065207.35
    206 rdf:rest Nb249ec0605f64f4b8f54dc7be333b867
    207 Nb7bab2d86afd43abbb3f8cad09779682 rdf:first sg:person.01100032432.25
    208 rdf:rest N3dd4d7b329e44f878a27b6ebb7ec2382
    209 Nb89509b428d745209d7db17e27dd1e71 rdf:first sg:person.01162141537.04
    210 rdf:rest N34a2d2e93af945b38b1c6c5707e1b031
    211 Nbe041894c6a2429e9e01870238f4303a rdf:first sg:person.01353167646.44
    212 rdf:rest Nf60d93dcb12b4421a854c410d6ccf4e5
    213 Nbf470abc97634fe4855f4c76e625a1bf schema:name dimensions_id
    214 schema:value pub.1020976552
    215 rdf:type schema:PropertyValue
    216 Nc2ab56b565ef48979ba7d3d1bb58c39a schema:name pubmed_id
    217 schema:value 26550473
    218 rdf:type schema:PropertyValue
    219 Nc51bc86c9b9847c2a7310ced1d7092f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    220 schema:name RNA Splicing
    221 rdf:type schema:DefinedTerm
    222 Nc5ba11e5c11c413e92e9d28b29c25d07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    223 schema:name Alphapapillomavirus
    224 rdf:type schema:DefinedTerm
    225 Nc7f8c99112944bc7850d8e65da2d1e81 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    226 schema:name Sequence Analysis, RNA
    227 rdf:type schema:DefinedTerm
    228 Nc8d64f98c71b4330a989d9addd4d386d schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    229 schema:familyName Rao
    230 schema:givenName Weijian
    231 rdf:type schema:Person
    232 Ncf725db57abd404bbd8d7903e929a35d rdf:first Ndb8ec34f3e014a029160599e389659ab
    233 rdf:rest Na558ca55cc3d46a4923b1540a5f73b11
    234 Ncfec86d3b57b4ba49831b11bb07df38d rdf:first sg:person.01371136134.43
    235 rdf:rest Ncf725db57abd404bbd8d7903e929a35d
    236 Nd0b21a891af3487a889d8f3d2f9d0353 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    237 schema:name Neoplasms
    238 rdf:type schema:DefinedTerm
    239 Nd25146cd576246e4a8e31d9ba3e0a84a rdf:first N4de17ae941f64ace9d7f365ef1489c93
    240 rdf:rest N46c6d659593140b9b90af8b7f999993d
    241 Nd7621fec47404df58d10fefd22a343b3 schema:name Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China
    242 rdf:type schema:Organization
    243 Ndb8ec34f3e014a029160599e389659ab schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    244 schema:familyName Li
    245 schema:givenName Yang
    246 rdf:type schema:Person
    247 Ndd6d239b507149ff9875356c4a3c8741 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    248 schema:name HeLa Cells
    249 rdf:type schema:DefinedTerm
    250 Nde256961f76746969a349e66e6af62c4 rdf:first sg:person.01303526064.50
    251 rdf:rest Nfe57df50ffc74c79b53c18e0c973d96b
    252 Ndfd4bab45a8d4e8f9a61052fde0d9001 rdf:first sg:person.01372000452.04
    253 rdf:rest N6e7e82b1e41c47b1a5914533bf9eab03
    254 Ne3551195d83c47138a36f74abe271577 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    255 schema:familyName Zhang
    256 schema:givenName Jie
    257 rdf:type schema:Person
    258 Ne8bc988d47aa49349f74ee5507a37b1d rdf:first N67a14e7e35854267af453e0f9e7588b1
    259 rdf:rest Nb6d0e38c3adf44e1b2690bd9b2b58bc6
    260 Neb04f0e180ac4c90a82029336f20da72 schema:affiliation Nd7621fec47404df58d10fefd22a343b3
    261 schema:familyName Wang
    262 schema:givenName Ling
    263 rdf:type schema:Person
    264 Ned7b19e2b72842aeb2583b9740bcb54f rdf:first sg:person.0762053777.20
    265 rdf:rest Ndfd4bab45a8d4e8f9a61052fde0d9001
    266 Nef4f9653c90c4113a1050d4214b5c14f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    267 schema:name Genetic Heterogeneity
    268 rdf:type schema:DefinedTerm
    269 Nf60d93dcb12b4421a854c410d6ccf4e5 rdf:first N9db0c15443c7473aa29f26109a4f9ad4
    270 rdf:rest N8cf654cdc69740d1a86cda6a1d2044c3
    271 Nfc8c5ea640084a349a9907a14714725d schema:name Springer Nature - SN SciGraph project
    272 rdf:type schema:Organization
    273 Nfe57df50ffc74c79b53c18e0c973d96b rdf:first sg:person.013612667131.55
    274 rdf:rest N488aab4aaa394b04b18bd1c8433b2c15
    275 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    276 schema:name Biological Sciences
    277 rdf:type schema:DefinedTerm
    278 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    279 schema:name Genetics
    280 rdf:type schema:DefinedTerm
    281 sg:grant.7015769 http://pending.schema.org/fundedItem sg:pub.10.1186/s13742-015-0091-4
    282 rdf:type schema:MonetaryGrant
    283 sg:grant.7200984 http://pending.schema.org/fundedItem sg:pub.10.1186/s13742-015-0091-4
    284 rdf:type schema:MonetaryGrant
    285 sg:journal.1047731 schema:issn 2047-217X
    286 schema:name GigaScience
    287 rdf:type schema:Periodical
    288 sg:person.01031604372.16 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
    289 schema:familyName Dean
    290 schema:givenName Michael
    291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031604372.16
    292 rdf:type schema:Person
    293 sg:person.01100032432.25 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
    294 schema:familyName Hou
    295 schema:givenName Yong
    296 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100032432.25
    297 rdf:type schema:Person
    298 sg:person.011026731327.79 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    299 schema:familyName Wu
    300 schema:givenName Liang
    301 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011026731327.79
    302 rdf:type schema:Person
    303 sg:person.01143540012.53 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    304 schema:familyName Xu
    305 schema:givenName Xun
    306 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143540012.53
    307 rdf:type schema:Person
    308 sg:person.01162141537.04 schema:affiliation https://www.grid.ac/institutes/grid.263826.b
    309 schema:familyName Zhao
    310 schema:givenName Zhikun
    311 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162141537.04
    312 rdf:type schema:Person
    313 sg:person.01231641167.20 schema:affiliation https://www.grid.ac/institutes/grid.13402.34
    314 schema:familyName Yang
    315 schema:givenName Huanming
    316 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231641167.20
    317 rdf:type schema:Person
    318 sg:person.01254477637.32 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    319 schema:familyName Xie
    320 schema:givenName Guoyun
    321 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254477637.32
    322 rdf:type schema:Person
    323 sg:person.01303526064.50 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    324 schema:familyName Li
    325 schema:givenName Bo
    326 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303526064.50
    327 rdf:type schema:Person
    328 sg:person.01353167646.44 schema:affiliation Nab1f89b5f3f443429bb6cbcf392d8ccc
    329 schema:familyName Chen
    330 schema:givenName Jianghao
    331 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353167646.44
    332 rdf:type schema:Person
    333 sg:person.013612667131.55 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
    334 schema:familyName Li
    335 schema:givenName Guibo
    336 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013612667131.55
    337 rdf:type schema:Person
    338 sg:person.01371136134.43 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    339 schema:familyName Mei
    340 schema:givenName Zhanlong
    341 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371136134.43
    342 rdf:type schema:Person
    343 sg:person.01372000452.04 schema:affiliation https://www.grid.ac/institutes/grid.1003.2
    344 schema:familyName Li
    345 schema:givenName Yingrui
    346 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372000452.04
    347 rdf:type schema:Person
    348 sg:person.0603065207.35 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    349 schema:familyName Wu
    350 schema:givenName Kui
    351 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603065207.35
    352 rdf:type schema:Person
    353 sg:person.0667212437.82 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    354 schema:familyName Yu
    355 schema:givenName Qichao
    356 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667212437.82
    357 rdf:type schema:Person
    358 sg:person.0707574237.39 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    359 schema:familyName Jiang
    360 schema:givenName Runze
    361 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707574237.39
    362 rdf:type schema:Person
    363 sg:person.0762053777.20 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    364 schema:familyName Zhang
    365 schema:givenName Xiuqing
    366 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762053777.20
    367 rdf:type schema:Person
    368 sg:pub.10.1007/s13277-014-2526-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023363208
    369 https://doi.org/10.1007/s13277-014-2526-4
    370 rdf:type schema:CreativeWork
    371 sg:pub.10.1038/35057062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042854081
    372 https://doi.org/10.1038/35057062
    373 rdf:type schema:CreativeWork
    374 sg:pub.10.1038/cr.2014.35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032435968
    375 https://doi.org/10.1038/cr.2014.35
    376 rdf:type schema:CreativeWork
    377 sg:pub.10.1038/nature09515 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029325111
    378 https://doi.org/10.1038/nature09515
    379 rdf:type schema:CreativeWork
    380 sg:pub.10.1038/nature09807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020406938
    381 https://doi.org/10.1038/nature09807
    382 rdf:type schema:CreativeWork
    383 sg:pub.10.1038/nature12064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040190826
    384 https://doi.org/10.1038/nature12064
    385 rdf:type schema:CreativeWork
    386 sg:pub.10.1038/nature12172 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035006736
    387 https://doi.org/10.1038/nature12172
    388 rdf:type schema:CreativeWork
    389 sg:pub.10.1038/nbt.1754 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019307928
    390 https://doi.org/10.1038/nbt.1754
    391 rdf:type schema:CreativeWork
    392 sg:pub.10.1038/nbt.2720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045584197
    393 https://doi.org/10.1038/nbt.2720
    394 rdf:type schema:CreativeWork
    395 sg:pub.10.1038/nbt.3102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016103324
    396 https://doi.org/10.1038/nbt.3102
    397 rdf:type schema:CreativeWork
    398 sg:pub.10.1038/ncomms3513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036430565
    399 https://doi.org/10.1038/ncomms3513
    400 rdf:type schema:CreativeWork
    401 sg:pub.10.1038/ng.3178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000968113
    402 https://doi.org/10.1038/ng.3178
    403 rdf:type schema:CreativeWork
    404 sg:pub.10.1038/nm.2415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051273515
    405 https://doi.org/10.1038/nm.2415
    406 rdf:type schema:CreativeWork
    407 sg:pub.10.1038/nmeth.1923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006541515
    408 https://doi.org/10.1038/nmeth.1923
    409 rdf:type schema:CreativeWork
    410 sg:pub.10.1038/nmeth.2772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029706604
    411 https://doi.org/10.1038/nmeth.2772
    412 rdf:type schema:CreativeWork
    413 sg:pub.10.1038/nprot.2012.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030124536
    414 https://doi.org/10.1038/nprot.2012.016
    415 rdf:type schema:CreativeWork
    416 sg:pub.10.1038/nprot.2014.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017950805
    417 https://doi.org/10.1038/nprot.2014.006
    418 rdf:type schema:CreativeWork
    419 sg:pub.10.1038/nrc2091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043783272
    420 https://doi.org/10.1038/nrc2091
    421 rdf:type schema:CreativeWork
    422 sg:pub.10.1038/nrc2961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042558338
    423 https://doi.org/10.1038/nrc2961
    424 rdf:type schema:CreativeWork
    425 sg:pub.10.1038/nrc3400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024896356
    426 https://doi.org/10.1038/nrc3400
    427 rdf:type schema:CreativeWork
    428 sg:pub.10.1038/nrc3449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039804755
    429 https://doi.org/10.1038/nrc3449
    430 rdf:type schema:CreativeWork
    431 sg:pub.10.1038/onc.2014.262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009744407
    432 https://doi.org/10.1038/onc.2014.262
    433 rdf:type schema:CreativeWork
    434 sg:pub.10.1038/sj.onc.1203134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016305716
    435 https://doi.org/10.1038/sj.onc.1203134
    436 rdf:type schema:CreativeWork
    437 sg:pub.10.1038/sj.onc.1204130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036918842
    438 https://doi.org/10.1038/sj.onc.1204130
    439 rdf:type schema:CreativeWork
    440 sg:pub.10.1186/1471-2105-9-559 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020312314
    441 https://doi.org/10.1186/1471-2105-9-559
    442 rdf:type schema:CreativeWork
    443 sg:pub.10.1186/1476-4598-10-61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006038094
    444 https://doi.org/10.1186/1476-4598-10-61
    445 rdf:type schema:CreativeWork
    446 sg:pub.10.1186/gb-2007-8-3-r39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051807491
    447 https://doi.org/10.1186/gb-2007-8-3-r39
    448 rdf:type schema:CreativeWork
    449 sg:pub.10.1186/gb-2009-10-3-r25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049583368
    450 https://doi.org/10.1186/gb-2009-10-3-r25
    451 rdf:type schema:CreativeWork
    452 sg:pub.10.1186/gb-2011-12-1-r6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050465130
    453 https://doi.org/10.1186/gb-2011-12-1-r6
    454 rdf:type schema:CreativeWork
    455 sg:pub.10.1186/gb-2011-12-8-r72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006381787
    456 https://doi.org/10.1186/gb-2011-12-8-r72
    457 rdf:type schema:CreativeWork
    458 https://app.dimensions.ai/details/publication/pub.1074493152 schema:CreativeWork
    459 https://app.dimensions.ai/details/publication/pub.1075179519 schema:CreativeWork
    460 https://app.dimensions.ai/details/publication/pub.1075570876 schema:CreativeWork
    461 https://app.dimensions.ai/details/publication/pub.1079008116 schema:CreativeWork
    462 https://app.dimensions.ai/details/publication/pub.1082847169 schema:CreativeWork
    463 https://doi.org/10.1002/ijc.24844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034369046
    464 rdf:type schema:CreativeWork
    465 https://doi.org/10.1016/0092-8674(90)90409-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040120250
    466 rdf:type schema:CreativeWork
    467 https://doi.org/10.1016/j.canlet.2011.02.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043991108
    468 rdf:type schema:CreativeWork
    469 https://doi.org/10.1016/j.ccr.2014.01.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002498840
    470 rdf:type schema:CreativeWork
    471 https://doi.org/10.1016/j.celrep.2014.08.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041908806
    472 rdf:type schema:CreativeWork
    473 https://doi.org/10.1016/j.chom.2014.02.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026644575
    474 rdf:type schema:CreativeWork
    475 https://doi.org/10.1016/j.chom.2014.02.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014449031
    476 rdf:type schema:CreativeWork
    477 https://doi.org/10.1016/j.ejogrb.2009.07.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049915789
    478 rdf:type schema:CreativeWork
    479 https://doi.org/10.1016/j.jhep.2006.05.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037562150
    480 rdf:type schema:CreativeWork
    481 https://doi.org/10.1016/j.molcel.2015.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022270616
    482 rdf:type schema:CreativeWork
    483 https://doi.org/10.1016/j.tig.2012.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009222806
    484 rdf:type schema:CreativeWork
    485 https://doi.org/10.1016/j.virol.2009.06.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041496962
    486 rdf:type schema:CreativeWork
    487 https://doi.org/10.1016/j.virol.2013.12.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020109637
    488 rdf:type schema:CreativeWork
    489 https://doi.org/10.1016/s0304-3835(01)00871-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037780457
    490 rdf:type schema:CreativeWork
    491 https://doi.org/10.1016/s1470-2045(09)70096-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009050725
    492 rdf:type schema:CreativeWork
    493 https://doi.org/10.1016/s1470-2045(12)70137-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037022536
    494 rdf:type schema:CreativeWork
    495 https://doi.org/10.1056/nejm199601113340213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016487843
    496 rdf:type schema:CreativeWork
    497 https://doi.org/10.1056/nejmoa021641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036284978
    498 rdf:type schema:CreativeWork
    499 https://doi.org/10.1073/pnas.0506580102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037705714
    500 rdf:type schema:CreativeWork
    501 https://doi.org/10.1091/mbc.02-02-0030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038504953
    502 rdf:type schema:CreativeWork
    503 https://doi.org/10.1091/mbc.02-02-0030. schema:sameAs https://app.dimensions.ai/details/publication/pub.1020036628
    504 rdf:type schema:CreativeWork
    505 https://doi.org/10.1093/bioinformatics/btp352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023014918
    506 rdf:type schema:CreativeWork
    507 https://doi.org/10.1093/bioinformatics/btp616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023247882
    508 rdf:type schema:CreativeWork
    509 https://doi.org/10.1093/bioinformatics/bts678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038581758
    510 rdf:type schema:CreativeWork
    511 https://doi.org/10.1093/nar/gkg786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000364966
    512 rdf:type schema:CreativeWork
    513 https://doi.org/10.1093/nar/gkt1196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015989191
    514 rdf:type schema:CreativeWork
    515 https://doi.org/10.1093/nar/gku1207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039030168
    516 rdf:type schema:CreativeWork
    517 https://doi.org/10.1101/gad.11.16.2090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026178926
    518 rdf:type schema:CreativeWork
    519 https://doi.org/10.1101/gr.135350.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012073457
    520 rdf:type schema:CreativeWork
    521 https://doi.org/10.1101/gr.161034.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018538260
    522 rdf:type schema:CreativeWork
    523 https://doi.org/10.1111/igc.0b013e3181af711e schema:sameAs https://app.dimensions.ai/details/publication/pub.1061838100
    524 rdf:type schema:CreativeWork
    525 https://doi.org/10.1126/science.1156725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062457660
    526 rdf:type schema:CreativeWork
    527 https://doi.org/10.1126/science.1254257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046815039
    528 rdf:type schema:CreativeWork
    529 https://doi.org/10.1128/jvi.01162-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027858553
    530 rdf:type schema:CreativeWork
    531 https://doi.org/10.1136/jclinpath-2014-202560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013869709
    532 rdf:type schema:CreativeWork
    533 https://doi.org/10.1158/1078-0432.ccr-05-2573 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012224313
    534 rdf:type schema:CreativeWork
    535 https://doi.org/10.1158/1078-0432.ccr-06-0571 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020795903
    536 rdf:type schema:CreativeWork
    537 https://doi.org/10.1158/1078-0432.ccr-14-0122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041393209
    538 rdf:type schema:CreativeWork
    539 https://doi.org/10.1289/ehp.95103s8259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064746153
    540 rdf:type schema:CreativeWork
    541 https://doi.org/10.1371/journal.pcbi.1002464 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050428866
    542 rdf:type schema:CreativeWork
    543 https://doi.org/10.1371/journal.pcbi.1003696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022849102
    544 rdf:type schema:CreativeWork
    545 https://doi.org/10.1371/journal.pone.0016266 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039493284
    546 rdf:type schema:CreativeWork
    547 https://doi.org/10.1371/journal.pone.0116151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020563858
    548 rdf:type schema:CreativeWork
    549 https://doi.org/10.1534/g3.113.005777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028964445
    550 rdf:type schema:CreativeWork
    551 https://doi.org/10.3892/ol.2013.1483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012344741
    552 rdf:type schema:CreativeWork
    553 https://doi.org/10.3892/or_00000729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071537513
    554 rdf:type schema:CreativeWork
    555 https://www.grid.ac/institutes/grid.1003.2 schema:alternateName University of Queensland
    556 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    557 Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia
    558 rdf:type schema:Organization
    559 https://www.grid.ac/institutes/grid.13402.34 schema:alternateName Zhejiang University
    560 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    561 James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China
    562 rdf:type schema:Organization
    563 https://www.grid.ac/institutes/grid.21155.32 schema:alternateName Beijing Genomics Institute
    564 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    565 The Guangdong Enterprise Key Laboratory of Human Disease Genomics, BGI-Shenzhen, 518083, Shenzhen, China
    566 rdf:type schema:Organization
    567 https://www.grid.ac/institutes/grid.263826.b schema:alternateName Southeast University
    568 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    569 School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, China
    570 State Key Laboratory of Bioelectronics, Southeast University, 210096, Nanjing, China
    571 rdf:type schema:Organization
    572 https://www.grid.ac/institutes/grid.410726.6 schema:alternateName University of Chinese Academy of Sciences
    573 schema:name BGI-Education Center, University of Chinese Academy of Sciences, 518083, Shenzhen, China
    574 BGI-Shenzhen, 518083, Shenzhen, China
    575 College of Life Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China
    576 rdf:type schema:Organization
    577 https://www.grid.ac/institutes/grid.48336.3a schema:alternateName National Cancer Institute
    578 schema:name Cancer and Inflammation Program, National Cancer Institute at Frederick, Building 560, 21702, Frederick, MD, USA
    579 rdf:type schema:Organization
    580 https://www.grid.ac/institutes/grid.5254.6 schema:alternateName University of Copenhagen
    581 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    582 Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark
    583 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...