Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Liang Wu, Xiaolong Zhang, Zhikun Zhao, Ling Wang, Bo Li, Guibo Li, Michael Dean, Qichao Yu, Yanhui Wang, Xinxin Lin, Weijian Rao, Zhanlong Mei, Yang Li, Runze Jiang, Huan Yang, Fuqiang Li, Guoyun Xie, Liqin Xu, Kui Wu, Jie Zhang, Jianghao Chen, Ting Wang, Karsten Kristiansen, Xiuqing Zhang, Yingrui Li, Huanming Yang, Jian Wang, Yong Hou, Xun Xu

ABSTRACT

BACKGROUND: Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line. RESULT: We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins. CONCLUSION: Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers. More... »

PAGES

51

References to SciGraph publications

  • 2001-02. Initial sequencing and analysis of the human genome in NATURE
  • 2013-08. The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line in NATURE
  • 2014-05. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus in CELL RESEARCH
  • 2014-12. C-Myc-activated long noncoding RNA CCAT1 promotes colon cancer cell proliferation and invasion in TUMOR BIOLOGY
  • 2009-03. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome in GENOME BIOLOGY
  • 2011-12. The HPV-16 E7 oncoprotein induces centriole multiplication through deregulation of Polo-like kinase 4 expression in MOLECULAR CANCER
  • 2008-12. WGCNA: an R package for weighted correlation network analysis in BMC BIOINFORMATICS
  • 2001-02. Regulation of the Cdc25A gene by the human papillomavirus Type 16 E7 oncogene in ONCOGENE
  • 2010-12. Why do viruses cause cancer? Highlights of the first century of human tumour virology in NATURE REVIEWS CANCER
  • 2011-01. Integrative genomics viewer in NATURE BIOTECHNOLOGY
  • 2014-01. Full-length RNA-seq from single cells using Smart-seq2 in NATURE PROTOCOLS
  • 2015-02. Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism in NATURE GENETICS
  • 2012-04. Fast gapped-read alignment with Bowtie 2 in NATURE METHODS
  • 2013-06. Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells in NATURE
  • 2011-04. Tumour evolution inferred by single-cell sequencing in NATURE
  • 2011-08. TopHat-Fusion: an algorithm for discovery of novel fusion transcripts in GENOME BIOLOGY
  • 1999-12. HPV-18 E6*I protein modulates the E6-directed degradation of p53 by binding to full-length HPV-18 E6 in ONCOGENE
  • 2007-04. The impact of translocations and gene fusions on cancer causation in NATURE REVIEWS CANCER
  • 2010-10-28. Distant metastasis occurs late during the genetic evolution of pancreatic cancer in NATURE
  • 2015-06. Single-cell gene expression signatures reveal melanoma cell heterogeneity in ONCOGENE
  • 2012-03-01. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks in NATURE PROTOCOLS
  • 2011-09. Stem cell gene expression programs influence clinical outcome in human leukemia in NATURE MEDICINE
  • 2011-01. Identification of fusion genes in breast cancer by paired-end RNA-sequencing in GENOME BIOLOGY
  • 2012-12. Human tumour viruses and the deregulation of cell polarity in cancer in NATURE REVIEWS CANCER
  • 2014-02. Quantitative single-cell RNA-seq with unique molecular identifiers in NATURE METHODS
  • 2015-03. Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells in NATURE BIOTECHNOLOGY
  • 2013-12. Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells in NATURE BIOTECHNOLOGY
  • 2007-03. Reactome: a knowledge base of biologic pathways and processes in GENOME BIOLOGY
  • 2013-02. Pathogenic mechanisms in HBV- and HCV-associated hepatocellular carcinoma in NATURE REVIEWS CANCER
  • 2013-12. The landscape of viral expression and host gene fusion and adaptation in human cancer in NATURE COMMUNICATIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4

    DOI

    http://dx.doi.org/10.1186/s13742-015-0091-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020976552

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26550473


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alphapapillomavirus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Heterogeneity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HeLa Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA Splicing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, RNA", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Liang", 
            "id": "sg:person.011026731327.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011026731327.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "College of Life Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xiaolong", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Southeast University", 
              "id": "https://www.grid.ac/institutes/grid.263826.b", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "State Key Laboratory of Bioelectronics, Southeast University, 210096, Nanjing, China", 
                "School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhao", 
            "givenName": "Zhikun", 
            "id": "sg:person.01162141537.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162141537.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi\u2019an, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Ling", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Bo", 
            "id": "sg:person.01303526064.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303526064.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Copenhagen", 
              "id": "https://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Guibo", 
            "id": "sg:person.013612667131.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013612667131.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Cancer Institute", 
              "id": "https://www.grid.ac/institutes/grid.48336.3a", 
              "name": [
                "Cancer and Inflammation Program, National Cancer Institute at Frederick, Building 560, 21702, Frederick, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dean", 
            "givenName": "Michael", 
            "id": "sg:person.01031604372.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031604372.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Chinese Academy of Sciences", 
              "id": "https://www.grid.ac/institutes/grid.410726.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "BGI-Education Center, University of Chinese Academy of Sciences, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Qichao", 
            "id": "sg:person.0667212437.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667212437.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Yanhui", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lin", 
            "givenName": "Xinxin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rao", 
            "givenName": "Weijian", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mei", 
            "givenName": "Zhanlong", 
            "id": "sg:person.01371136134.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371136134.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Yang", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jiang", 
            "givenName": "Runze", 
            "id": "sg:person.0707574237.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707574237.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Huan", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Fuqiang", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xie", 
            "givenName": "Guoyun", 
            "id": "sg:person.01254477637.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254477637.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Liqin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Kui", 
            "id": "sg:person.0603065207.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603065207.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Jie", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi\u2019an, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Jianghao", 
            "id": "sg:person.01353167646.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353167646.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi\u2019an, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Ting", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Copenhagen", 
              "id": "https://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kristiansen", 
            "givenName": "Karsten", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "The Guangdong Enterprise Key Laboratory of Human Disease Genomics, BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xiuqing", 
            "id": "sg:person.0762053777.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762053777.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Queensland", 
              "id": "https://www.grid.ac/institutes/grid.1003.2", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Yingrui", 
            "id": "sg:person.01372000452.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372000452.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Zhejiang University", 
              "id": "https://www.grid.ac/institutes/grid.13402.34", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Huanming", 
            "id": "sg:person.01231641167.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231641167.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Zhejiang University", 
              "id": "https://www.grid.ac/institutes/grid.13402.34", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Jian", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Copenhagen", 
              "id": "https://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China", 
                "Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hou", 
            "givenName": "Yong", 
            "id": "sg:person.01100032432.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100032432.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Beijing Genomics Institute", 
              "id": "https://www.grid.ac/institutes/grid.21155.32", 
              "name": [
                "BGI-Shenzhen, 518083, Shenzhen, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Xun", 
            "id": "sg:person.01143540012.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143540012.53"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1093/nar/gkg786", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000364966"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.3178", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000968113", 
              "https://doi.org/10.1038/ng.3178"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ccr.2014.01.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002498840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1476-4598-10-61", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006038094", 
              "https://doi.org/10.1186/1476-4598-10-61"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2011-12-8-r72", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006381787", 
              "https://doi.org/10.1186/gb-2011-12-8-r72"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1923", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006541515", 
              "https://doi.org/10.1038/nmeth.1923"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1470-2045(09)70096-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009050725"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tig.2012.07.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009222806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/onc.2014.262", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009744407", 
              "https://doi.org/10.1038/onc.2014.262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.135350.111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012073457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-05-2573", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012224313"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ol.2013.1483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012344741"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ol.2013.1483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012344741"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1136/jclinpath-2014-202560", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013869709"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.chom.2014.02.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014449031"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt1196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015989191"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.3102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016103324", 
              "https://doi.org/10.1038/nbt.3102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1203134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016305716", 
              "https://doi.org/10.1038/sj.onc.1203134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1203134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016305716", 
              "https://doi.org/10.1038/sj.onc.1203134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejm199601113340213", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016487843"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2014.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017950805", 
              "https://doi.org/10.1038/nprot.2014.006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.161034.113", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018538260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1754", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019307928", 
              "https://doi.org/10.1038/nbt.1754"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.02-02-0030.", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020036628"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virol.2013.12.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020109637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2105-9-559", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020312314", 
              "https://doi.org/10.1186/1471-2105-9-559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09807", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020406938", 
              "https://doi.org/10.1038/nature09807"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0116151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020563858"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-06-0571", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020795903"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2015.03.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022270616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pcbi.1003696", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022849102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp352", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023014918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp616", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023247882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s13277-014-2526-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023363208", 
              "https://doi.org/10.1007/s13277-014-2526-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc3400", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024896356", 
              "https://doi.org/10.1038/nrc3400"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.11.16.2090", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026178926"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.chom.2014.02.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026644575"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.01162-09", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027858553"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/g3.113.005777", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028964445"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/g3.113.005777", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028964445"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09515", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029325111", 
              "https://doi.org/10.1038/nature09515"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09515", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029325111", 
              "https://doi.org/10.1038/nature09515"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2772", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029706604", 
              "https://doi.org/10.1038/nmeth.2772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2012.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030124536", 
              "https://doi.org/10.1038/nprot.2012.016"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2014.35", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032435968", 
              "https://doi.org/10.1038/cr.2014.35"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.24844", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034369046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.24844", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034369046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12172", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035006736", 
              "https://doi.org/10.1038/nature12172"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejmoa021641", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036284978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms3513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036430565", 
              "https://doi.org/10.1038/ncomms3513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1204130", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036918842", 
              "https://doi.org/10.1038/sj.onc.1204130"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1204130", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036918842", 
              "https://doi.org/10.1038/sj.onc.1204130"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1470-2045(12)70137-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037022536"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jhep.2006.05.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037562150"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0304-3835(01)00871-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037780457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.02-02-0030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038504953"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/bts678", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038581758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gku1207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039030168"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0016266", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039493284"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc3449", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039804755", 
              "https://doi.org/10.1038/nrc3449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(90)90409-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040120250"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12064", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040190826", 
              "https://doi.org/10.1038/nature12064"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-14-0122", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041393209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virol.2009.06.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041496962"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2014.08.029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041908806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc2961", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042558338", 
              "https://doi.org/10.1038/nrc2961"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc2961", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042558338", 
              "https://doi.org/10.1038/nrc2961"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35057062", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042854081", 
              "https://doi.org/10.1038/35057062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35057062", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042854081", 
              "https://doi.org/10.1038/35057062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc2091", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043783272", 
              "https://doi.org/10.1038/nrc2091"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.canlet.2011.02.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043991108"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045584197", 
              "https://doi.org/10.1038/nbt.2720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1254257", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046815039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2009-10-3-r25", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049583368", 
              "https://doi.org/10.1186/gb-2009-10-3-r25"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ejogrb.2009.07.012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049915789"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pcbi.1002464", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050428866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2011-12-1-r6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050465130", 
              "https://doi.org/10.1186/gb-2011-12-1-r6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.2415", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051273515", 
              "https://doi.org/10.1038/nm.2415"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2007-8-3-r39", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051807491", 
              "https://doi.org/10.1186/gb-2007-8-3-r39"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/igc.0b013e3181af711e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061838100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1156725", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062457660"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1289/ehp.95103s8259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064746153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/or_00000729", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071537513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074493152", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075179519", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075570876", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079008116", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082847169", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2015-12", 
        "datePublishedReg": "2015-12-01", 
        "description": "BACKGROUND: Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line.\nRESULT: We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins.\nCONCLUSION: Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s13742-015-0091-4", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.7015769", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.7200984", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1047731", 
            "issn": [
              "2047-217X"
            ], 
            "name": "GigaScience", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "4"
          }
        ], 
        "name": "Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells", 
        "pagination": "51", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "63f19f1897e987425f951bfbac94bb26c82c3e35214d3f39676ca24848a101fc"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "26550473"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101596872"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s13742-015-0091-4"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1020976552"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s13742-015-0091-4", 
          "https://app.dimensions.ai/details/publication/pub.1020976552"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T01:09", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000521.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1186%2Fs13742-015-0091-4"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13742-015-0091-4'


     

    This table displays all metadata directly associated to this object as RDF triples.

    583 TRIPLES      21 PREDICATES      117 URIs      28 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s13742-015-0091-4 schema:about N0d7549030d944e00a765049a9a682998
    2 N310f9ad2dccb4c1098065bc31f915db3
    3 N4e2e764d6f0442d1a590718c20e30f4f
    4 N52579024aa9846edb067c73af4bb95af
    5 N62d2c3830d614ed2881f462815ea5673
    6 N6da514af11aa4bd68f84b9cab9f2ae26
    7 Ne23719fc10a64cc59d981e7d7f6f5983
    8 anzsrc-for:06
    9 anzsrc-for:0604
    10 schema:author N32dee548313548d3990036cef73f213e
    11 schema:citation sg:pub.10.1007/s13277-014-2526-4
    12 sg:pub.10.1038/35057062
    13 sg:pub.10.1038/cr.2014.35
    14 sg:pub.10.1038/nature09515
    15 sg:pub.10.1038/nature09807
    16 sg:pub.10.1038/nature12064
    17 sg:pub.10.1038/nature12172
    18 sg:pub.10.1038/nbt.1754
    19 sg:pub.10.1038/nbt.2720
    20 sg:pub.10.1038/nbt.3102
    21 sg:pub.10.1038/ncomms3513
    22 sg:pub.10.1038/ng.3178
    23 sg:pub.10.1038/nm.2415
    24 sg:pub.10.1038/nmeth.1923
    25 sg:pub.10.1038/nmeth.2772
    26 sg:pub.10.1038/nprot.2012.016
    27 sg:pub.10.1038/nprot.2014.006
    28 sg:pub.10.1038/nrc2091
    29 sg:pub.10.1038/nrc2961
    30 sg:pub.10.1038/nrc3400
    31 sg:pub.10.1038/nrc3449
    32 sg:pub.10.1038/onc.2014.262
    33 sg:pub.10.1038/sj.onc.1203134
    34 sg:pub.10.1038/sj.onc.1204130
    35 sg:pub.10.1186/1471-2105-9-559
    36 sg:pub.10.1186/1476-4598-10-61
    37 sg:pub.10.1186/gb-2007-8-3-r39
    38 sg:pub.10.1186/gb-2009-10-3-r25
    39 sg:pub.10.1186/gb-2011-12-1-r6
    40 sg:pub.10.1186/gb-2011-12-8-r72
    41 https://app.dimensions.ai/details/publication/pub.1074493152
    42 https://app.dimensions.ai/details/publication/pub.1075179519
    43 https://app.dimensions.ai/details/publication/pub.1075570876
    44 https://app.dimensions.ai/details/publication/pub.1079008116
    45 https://app.dimensions.ai/details/publication/pub.1082847169
    46 https://doi.org/10.1002/ijc.24844
    47 https://doi.org/10.1016/0092-8674(90)90409-8
    48 https://doi.org/10.1016/j.canlet.2011.02.007
    49 https://doi.org/10.1016/j.ccr.2014.01.030
    50 https://doi.org/10.1016/j.celrep.2014.08.029
    51 https://doi.org/10.1016/j.chom.2014.02.010
    52 https://doi.org/10.1016/j.chom.2014.02.011
    53 https://doi.org/10.1016/j.ejogrb.2009.07.012
    54 https://doi.org/10.1016/j.jhep.2006.05.013
    55 https://doi.org/10.1016/j.molcel.2015.03.005
    56 https://doi.org/10.1016/j.tig.2012.07.003
    57 https://doi.org/10.1016/j.virol.2009.06.001
    58 https://doi.org/10.1016/j.virol.2013.12.011
    59 https://doi.org/10.1016/s0304-3835(01)00871-0
    60 https://doi.org/10.1016/s1470-2045(09)70096-8
    61 https://doi.org/10.1016/s1470-2045(12)70137-7
    62 https://doi.org/10.1056/nejm199601113340213
    63 https://doi.org/10.1056/nejmoa021641
    64 https://doi.org/10.1073/pnas.0506580102
    65 https://doi.org/10.1091/mbc.02-02-0030
    66 https://doi.org/10.1091/mbc.02-02-0030.
    67 https://doi.org/10.1093/bioinformatics/btp352
    68 https://doi.org/10.1093/bioinformatics/btp616
    69 https://doi.org/10.1093/bioinformatics/bts678
    70 https://doi.org/10.1093/nar/gkg786
    71 https://doi.org/10.1093/nar/gkt1196
    72 https://doi.org/10.1093/nar/gku1207
    73 https://doi.org/10.1101/gad.11.16.2090
    74 https://doi.org/10.1101/gr.135350.111
    75 https://doi.org/10.1101/gr.161034.113
    76 https://doi.org/10.1111/igc.0b013e3181af711e
    77 https://doi.org/10.1126/science.1156725
    78 https://doi.org/10.1126/science.1254257
    79 https://doi.org/10.1128/jvi.01162-09
    80 https://doi.org/10.1136/jclinpath-2014-202560
    81 https://doi.org/10.1158/1078-0432.ccr-05-2573
    82 https://doi.org/10.1158/1078-0432.ccr-06-0571
    83 https://doi.org/10.1158/1078-0432.ccr-14-0122
    84 https://doi.org/10.1289/ehp.95103s8259
    85 https://doi.org/10.1371/journal.pcbi.1002464
    86 https://doi.org/10.1371/journal.pcbi.1003696
    87 https://doi.org/10.1371/journal.pone.0016266
    88 https://doi.org/10.1371/journal.pone.0116151
    89 https://doi.org/10.1534/g3.113.005777
    90 https://doi.org/10.3892/ol.2013.1483
    91 https://doi.org/10.3892/or_00000729
    92 schema:datePublished 2015-12
    93 schema:datePublishedReg 2015-12-01
    94 schema:description BACKGROUND: Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line. RESULT: We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins. CONCLUSION: Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers.
    95 schema:genre research_article
    96 schema:inLanguage en
    97 schema:isAccessibleForFree true
    98 schema:isPartOf N27d97e3beab34d298786ef9e28a9ef8c
    99 N89e779d4e669427ea2a82c0e995ab45a
    100 sg:journal.1047731
    101 schema:name Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells
    102 schema:pagination 51
    103 schema:productId N000458faedae4c64a1f76df9727b44e7
    104 N2aa76f10156c4bf6a4adbdf5d585fec7
    105 N681cc9c52ca347758d029390e7f8496d
    106 N86dc9284f2ab46dd8154cfa55ec9c082
    107 Nb97a8570e2bb4617931176ed8ca302f3
    108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020976552
    109 https://doi.org/10.1186/s13742-015-0091-4
    110 schema:sdDatePublished 2019-04-11T01:09
    111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    112 schema:sdPublisher N6559ec175bec4e63b12e746a5ff2b5bd
    113 schema:url http://link.springer.com/10.1186%2Fs13742-015-0091-4
    114 sgo:license sg:explorer/license/
    115 sgo:sdDataset articles
    116 rdf:type schema:ScholarlyArticle
    117 N000458faedae4c64a1f76df9727b44e7 schema:name doi
    118 schema:value 10.1186/s13742-015-0091-4
    119 rdf:type schema:PropertyValue
    120 N024ed0786aef413da19a4f64f93eaffd rdf:first sg:person.01353167646.44
    121 rdf:rest Nf4589fbe2bf64f09905bc27cdec616c9
    122 N066f979fed654bac8b4e2ad21478eb2a rdf:first N2b731356096742aeb66115912e680757
    123 rdf:rest N632d58afcdb344938ff9e6e3c1658d48
    124 N0b49287b951b4b8fa67c3e94de0ffc33 schema:affiliation https://www.grid.ac/institutes/grid.13402.34
    125 schema:familyName Wang
    126 schema:givenName Jian
    127 rdf:type schema:Person
    128 N0d7549030d944e00a765049a9a682998 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name RNA Splicing
    130 rdf:type schema:DefinedTerm
    131 N138f075ef4134d46b1dd5cade7b240cc schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    132 schema:familyName Lin
    133 schema:givenName Xinxin
    134 rdf:type schema:Person
    135 N172f565d8837433382d052f35bfc0239 rdf:first N138f075ef4134d46b1dd5cade7b240cc
    136 rdf:rest Nc7225dba70aa452bab1da1a967dd46d3
    137 N186bb9c6a6d144baa7f43213bd1a9f1f rdf:first Nacfa5460f166489aa703de05eca50cc8
    138 rdf:rest N024ed0786aef413da19a4f64f93eaffd
    139 N1d1e69c0f4284e1daeb783dbd2b5b8ac rdf:first sg:person.013612667131.55
    140 rdf:rest Nb7b5733adc554aa4a4fa6fc9d7aa2c00
    141 N1df7c7832d474e0892b50f7d634d2175 schema:affiliation N2cd0bf42fbb140a7a56d551d33ee0405
    142 schema:familyName Wang
    143 schema:givenName Ting
    144 rdf:type schema:Person
    145 N2698dd5c7fb546799ce7af6d5da67a4b schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    146 schema:familyName Li
    147 schema:givenName Yang
    148 rdf:type schema:Person
    149 N27d97e3beab34d298786ef9e28a9ef8c schema:volumeNumber 4
    150 rdf:type schema:PublicationVolume
    151 N2aa76f10156c4bf6a4adbdf5d585fec7 schema:name readcube_id
    152 schema:value 63f19f1897e987425f951bfbac94bb26c82c3e35214d3f39676ca24848a101fc
    153 rdf:type schema:PropertyValue
    154 N2b731356096742aeb66115912e680757 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    155 schema:familyName Zhang
    156 schema:givenName Xiaolong
    157 rdf:type schema:Person
    158 N2cd0bf42fbb140a7a56d551d33ee0405 schema:name Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China
    159 rdf:type schema:Organization
    160 N310f9ad2dccb4c1098065bc31f915db3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Genetic Heterogeneity
    162 rdf:type schema:DefinedTerm
    163 N32dee548313548d3990036cef73f213e rdf:first sg:person.011026731327.79
    164 rdf:rest N066f979fed654bac8b4e2ad21478eb2a
    165 N3930c5555e544921ac5f6b9b7b81b5f3 rdf:first Nbe180100ca954f1b8a1f2fc96a82ee82
    166 rdf:rest Ndfd59de731c444d0959b990f1b43d284
    167 N3c1d426352ec4cfd977122e64e2acd8a schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    168 schema:familyName Xu
    169 schema:givenName Liqin
    170 rdf:type schema:Person
    171 N3c7d256a67c94baea1eadff68f1fec23 rdf:first N2698dd5c7fb546799ce7af6d5da67a4b
    172 rdf:rest N4bdef682773f420f8b18a69db5db754b
    173 N4a4827c5e68245b79106d64112ae6dc1 rdf:first N3c1d426352ec4cfd977122e64e2acd8a
    174 rdf:rest N57b6bf43520345cdb2e066b688041463
    175 N4bdef682773f420f8b18a69db5db754b rdf:first sg:person.0707574237.39
    176 rdf:rest N73bae5ac746d4c3b94eda6ca532389af
    177 N4e2e764d6f0442d1a590718c20e30f4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    178 schema:name Neoplasms
    179 rdf:type schema:DefinedTerm
    180 N4fbb81f953294b4a9a0a74a7f973af3d rdf:first N81b92e804f7243779aa02984e2a6e5c0
    181 rdf:rest Nd4454c86dadf49cbb8c12b9a1484edf2
    182 N52579024aa9846edb067c73af4bb95af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name HeLa Cells
    184 rdf:type schema:DefinedTerm
    185 N57b6bf43520345cdb2e066b688041463 rdf:first sg:person.0603065207.35
    186 rdf:rest N186bb9c6a6d144baa7f43213bd1a9f1f
    187 N62d2c3830d614ed2881f462815ea5673 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name Sequence Analysis, RNA
    189 rdf:type schema:DefinedTerm
    190 N632d58afcdb344938ff9e6e3c1658d48 rdf:first sg:person.01162141537.04
    191 rdf:rest N3930c5555e544921ac5f6b9b7b81b5f3
    192 N6559ec175bec4e63b12e746a5ff2b5bd schema:name Springer Nature - SN SciGraph project
    193 rdf:type schema:Organization
    194 N65f750024fc147179fb3873fc0ae3d34 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    195 schema:familyName Rao
    196 schema:givenName Weijian
    197 rdf:type schema:Person
    198 N681cc9c52ca347758d029390e7f8496d schema:name dimensions_id
    199 schema:value pub.1020976552
    200 rdf:type schema:PropertyValue
    201 N6c382d0afec6405295df339fd61cfcbb rdf:first sg:person.01143540012.53
    202 rdf:rest rdf:nil
    203 N6d721dc845b84b81b82179333e3c26ab rdf:first sg:person.01231641167.20
    204 rdf:rest Na6375d8e36ac4f99841fd99436935389
    205 N6da514af11aa4bd68f84b9cab9f2ae26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Humans
    207 rdf:type schema:DefinedTerm
    208 N6e7a4a719c45453ea1f05ef6b5bcb3f6 rdf:first sg:person.0762053777.20
    209 rdf:rest N9bf4489d9f2e4631bc1105fc5056cf7b
    210 N73bae5ac746d4c3b94eda6ca532389af rdf:first N8ae2d822156a462483f18b00080116c5
    211 rdf:rest N4fbb81f953294b4a9a0a74a7f973af3d
    212 N81b92e804f7243779aa02984e2a6e5c0 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    213 schema:familyName Li
    214 schema:givenName Fuqiang
    215 rdf:type schema:Person
    216 N86dc9284f2ab46dd8154cfa55ec9c082 schema:name nlm_unique_id
    217 schema:value 101596872
    218 rdf:type schema:PropertyValue
    219 N89e779d4e669427ea2a82c0e995ab45a schema:issueNumber 1
    220 rdf:type schema:PublicationIssue
    221 N8ae2d822156a462483f18b00080116c5 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    222 schema:familyName Yang
    223 schema:givenName Huan
    224 rdf:type schema:Person
    225 N8bd08fe35ea14eaca28954d73a2f3dd1 schema:name Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China
    226 rdf:type schema:Organization
    227 N8f1f94638ed047d3b86c2317376140a4 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
    228 schema:familyName Kristiansen
    229 schema:givenName Karsten
    230 rdf:type schema:Person
    231 N947e9f9dc0974af598d1def2a235c6b3 rdf:first N8f1f94638ed047d3b86c2317376140a4
    232 rdf:rest N6e7a4a719c45453ea1f05ef6b5bcb3f6
    233 N9bf4489d9f2e4631bc1105fc5056cf7b rdf:first sg:person.01372000452.04
    234 rdf:rest N6d721dc845b84b81b82179333e3c26ab
    235 Na6375d8e36ac4f99841fd99436935389 rdf:first N0b49287b951b4b8fa67c3e94de0ffc33
    236 rdf:rest Nb9ea033ef2cb4fd182807c074d610349
    237 Nacfa5460f166489aa703de05eca50cc8 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    238 schema:familyName Zhang
    239 schema:givenName Jie
    240 rdf:type schema:Person
    241 Naf09f62ee359403a85850c596c14072d schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    242 schema:familyName Wang
    243 schema:givenName Yanhui
    244 rdf:type schema:Person
    245 Nb17818b1fb744c7ba3eba1716af186f0 rdf:first sg:person.01371136134.43
    246 rdf:rest N3c7d256a67c94baea1eadff68f1fec23
    247 Nb7b5733adc554aa4a4fa6fc9d7aa2c00 rdf:first sg:person.01031604372.16
    248 rdf:rest Ncbc481f6674d436ba3ea20b73d9f8c76
    249 Nb92ec865251d4d96bb008b64afced676 rdf:first Naf09f62ee359403a85850c596c14072d
    250 rdf:rest N172f565d8837433382d052f35bfc0239
    251 Nb97a8570e2bb4617931176ed8ca302f3 schema:name pubmed_id
    252 schema:value 26550473
    253 rdf:type schema:PropertyValue
    254 Nb9ea033ef2cb4fd182807c074d610349 rdf:first sg:person.01100032432.25
    255 rdf:rest N6c382d0afec6405295df339fd61cfcbb
    256 Nbe180100ca954f1b8a1f2fc96a82ee82 schema:affiliation N8bd08fe35ea14eaca28954d73a2f3dd1
    257 schema:familyName Wang
    258 schema:givenName Ling
    259 rdf:type schema:Person
    260 Nc7225dba70aa452bab1da1a967dd46d3 rdf:first N65f750024fc147179fb3873fc0ae3d34
    261 rdf:rest Nb17818b1fb744c7ba3eba1716af186f0
    262 Ncbc481f6674d436ba3ea20b73d9f8c76 rdf:first sg:person.0667212437.82
    263 rdf:rest Nb92ec865251d4d96bb008b64afced676
    264 Nd4454c86dadf49cbb8c12b9a1484edf2 rdf:first sg:person.01254477637.32
    265 rdf:rest N4a4827c5e68245b79106d64112ae6dc1
    266 Ndfd59de731c444d0959b990f1b43d284 rdf:first sg:person.01303526064.50
    267 rdf:rest N1d1e69c0f4284e1daeb783dbd2b5b8ac
    268 Ne23719fc10a64cc59d981e7d7f6f5983 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    269 schema:name Alphapapillomavirus
    270 rdf:type schema:DefinedTerm
    271 Nf4589fbe2bf64f09905bc27cdec616c9 rdf:first N1df7c7832d474e0892b50f7d634d2175
    272 rdf:rest N947e9f9dc0974af598d1def2a235c6b3
    273 Nfb4c0d87ba7d43a3b215d73145e048d1 schema:name Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China
    274 rdf:type schema:Organization
    275 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    276 schema:name Biological Sciences
    277 rdf:type schema:DefinedTerm
    278 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    279 schema:name Genetics
    280 rdf:type schema:DefinedTerm
    281 sg:grant.7015769 http://pending.schema.org/fundedItem sg:pub.10.1186/s13742-015-0091-4
    282 rdf:type schema:MonetaryGrant
    283 sg:grant.7200984 http://pending.schema.org/fundedItem sg:pub.10.1186/s13742-015-0091-4
    284 rdf:type schema:MonetaryGrant
    285 sg:journal.1047731 schema:issn 2047-217X
    286 schema:name GigaScience
    287 rdf:type schema:Periodical
    288 sg:person.01031604372.16 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
    289 schema:familyName Dean
    290 schema:givenName Michael
    291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031604372.16
    292 rdf:type schema:Person
    293 sg:person.01100032432.25 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
    294 schema:familyName Hou
    295 schema:givenName Yong
    296 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100032432.25
    297 rdf:type schema:Person
    298 sg:person.011026731327.79 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    299 schema:familyName Wu
    300 schema:givenName Liang
    301 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011026731327.79
    302 rdf:type schema:Person
    303 sg:person.01143540012.53 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    304 schema:familyName Xu
    305 schema:givenName Xun
    306 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143540012.53
    307 rdf:type schema:Person
    308 sg:person.01162141537.04 schema:affiliation https://www.grid.ac/institutes/grid.263826.b
    309 schema:familyName Zhao
    310 schema:givenName Zhikun
    311 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162141537.04
    312 rdf:type schema:Person
    313 sg:person.01231641167.20 schema:affiliation https://www.grid.ac/institutes/grid.13402.34
    314 schema:familyName Yang
    315 schema:givenName Huanming
    316 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231641167.20
    317 rdf:type schema:Person
    318 sg:person.01254477637.32 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    319 schema:familyName Xie
    320 schema:givenName Guoyun
    321 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254477637.32
    322 rdf:type schema:Person
    323 sg:person.01303526064.50 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    324 schema:familyName Li
    325 schema:givenName Bo
    326 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303526064.50
    327 rdf:type schema:Person
    328 sg:person.01353167646.44 schema:affiliation Nfb4c0d87ba7d43a3b215d73145e048d1
    329 schema:familyName Chen
    330 schema:givenName Jianghao
    331 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353167646.44
    332 rdf:type schema:Person
    333 sg:person.013612667131.55 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
    334 schema:familyName Li
    335 schema:givenName Guibo
    336 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013612667131.55
    337 rdf:type schema:Person
    338 sg:person.01371136134.43 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    339 schema:familyName Mei
    340 schema:givenName Zhanlong
    341 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371136134.43
    342 rdf:type schema:Person
    343 sg:person.01372000452.04 schema:affiliation https://www.grid.ac/institutes/grid.1003.2
    344 schema:familyName Li
    345 schema:givenName Yingrui
    346 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372000452.04
    347 rdf:type schema:Person
    348 sg:person.0603065207.35 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    349 schema:familyName Wu
    350 schema:givenName Kui
    351 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603065207.35
    352 rdf:type schema:Person
    353 sg:person.0667212437.82 schema:affiliation https://www.grid.ac/institutes/grid.410726.6
    354 schema:familyName Yu
    355 schema:givenName Qichao
    356 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667212437.82
    357 rdf:type schema:Person
    358 sg:person.0707574237.39 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    359 schema:familyName Jiang
    360 schema:givenName Runze
    361 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707574237.39
    362 rdf:type schema:Person
    363 sg:person.0762053777.20 schema:affiliation https://www.grid.ac/institutes/grid.21155.32
    364 schema:familyName Zhang
    365 schema:givenName Xiuqing
    366 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762053777.20
    367 rdf:type schema:Person
    368 sg:pub.10.1007/s13277-014-2526-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023363208
    369 https://doi.org/10.1007/s13277-014-2526-4
    370 rdf:type schema:CreativeWork
    371 sg:pub.10.1038/35057062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042854081
    372 https://doi.org/10.1038/35057062
    373 rdf:type schema:CreativeWork
    374 sg:pub.10.1038/cr.2014.35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032435968
    375 https://doi.org/10.1038/cr.2014.35
    376 rdf:type schema:CreativeWork
    377 sg:pub.10.1038/nature09515 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029325111
    378 https://doi.org/10.1038/nature09515
    379 rdf:type schema:CreativeWork
    380 sg:pub.10.1038/nature09807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020406938
    381 https://doi.org/10.1038/nature09807
    382 rdf:type schema:CreativeWork
    383 sg:pub.10.1038/nature12064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040190826
    384 https://doi.org/10.1038/nature12064
    385 rdf:type schema:CreativeWork
    386 sg:pub.10.1038/nature12172 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035006736
    387 https://doi.org/10.1038/nature12172
    388 rdf:type schema:CreativeWork
    389 sg:pub.10.1038/nbt.1754 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019307928
    390 https://doi.org/10.1038/nbt.1754
    391 rdf:type schema:CreativeWork
    392 sg:pub.10.1038/nbt.2720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045584197
    393 https://doi.org/10.1038/nbt.2720
    394 rdf:type schema:CreativeWork
    395 sg:pub.10.1038/nbt.3102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016103324
    396 https://doi.org/10.1038/nbt.3102
    397 rdf:type schema:CreativeWork
    398 sg:pub.10.1038/ncomms3513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036430565
    399 https://doi.org/10.1038/ncomms3513
    400 rdf:type schema:CreativeWork
    401 sg:pub.10.1038/ng.3178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000968113
    402 https://doi.org/10.1038/ng.3178
    403 rdf:type schema:CreativeWork
    404 sg:pub.10.1038/nm.2415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051273515
    405 https://doi.org/10.1038/nm.2415
    406 rdf:type schema:CreativeWork
    407 sg:pub.10.1038/nmeth.1923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006541515
    408 https://doi.org/10.1038/nmeth.1923
    409 rdf:type schema:CreativeWork
    410 sg:pub.10.1038/nmeth.2772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029706604
    411 https://doi.org/10.1038/nmeth.2772
    412 rdf:type schema:CreativeWork
    413 sg:pub.10.1038/nprot.2012.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030124536
    414 https://doi.org/10.1038/nprot.2012.016
    415 rdf:type schema:CreativeWork
    416 sg:pub.10.1038/nprot.2014.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017950805
    417 https://doi.org/10.1038/nprot.2014.006
    418 rdf:type schema:CreativeWork
    419 sg:pub.10.1038/nrc2091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043783272
    420 https://doi.org/10.1038/nrc2091
    421 rdf:type schema:CreativeWork
    422 sg:pub.10.1038/nrc2961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042558338
    423 https://doi.org/10.1038/nrc2961
    424 rdf:type schema:CreativeWork
    425 sg:pub.10.1038/nrc3400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024896356
    426 https://doi.org/10.1038/nrc3400
    427 rdf:type schema:CreativeWork
    428 sg:pub.10.1038/nrc3449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039804755
    429 https://doi.org/10.1038/nrc3449
    430 rdf:type schema:CreativeWork
    431 sg:pub.10.1038/onc.2014.262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009744407
    432 https://doi.org/10.1038/onc.2014.262
    433 rdf:type schema:CreativeWork
    434 sg:pub.10.1038/sj.onc.1203134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016305716
    435 https://doi.org/10.1038/sj.onc.1203134
    436 rdf:type schema:CreativeWork
    437 sg:pub.10.1038/sj.onc.1204130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036918842
    438 https://doi.org/10.1038/sj.onc.1204130
    439 rdf:type schema:CreativeWork
    440 sg:pub.10.1186/1471-2105-9-559 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020312314
    441 https://doi.org/10.1186/1471-2105-9-559
    442 rdf:type schema:CreativeWork
    443 sg:pub.10.1186/1476-4598-10-61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006038094
    444 https://doi.org/10.1186/1476-4598-10-61
    445 rdf:type schema:CreativeWork
    446 sg:pub.10.1186/gb-2007-8-3-r39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051807491
    447 https://doi.org/10.1186/gb-2007-8-3-r39
    448 rdf:type schema:CreativeWork
    449 sg:pub.10.1186/gb-2009-10-3-r25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049583368
    450 https://doi.org/10.1186/gb-2009-10-3-r25
    451 rdf:type schema:CreativeWork
    452 sg:pub.10.1186/gb-2011-12-1-r6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050465130
    453 https://doi.org/10.1186/gb-2011-12-1-r6
    454 rdf:type schema:CreativeWork
    455 sg:pub.10.1186/gb-2011-12-8-r72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006381787
    456 https://doi.org/10.1186/gb-2011-12-8-r72
    457 rdf:type schema:CreativeWork
    458 https://app.dimensions.ai/details/publication/pub.1074493152 schema:CreativeWork
    459 https://app.dimensions.ai/details/publication/pub.1075179519 schema:CreativeWork
    460 https://app.dimensions.ai/details/publication/pub.1075570876 schema:CreativeWork
    461 https://app.dimensions.ai/details/publication/pub.1079008116 schema:CreativeWork
    462 https://app.dimensions.ai/details/publication/pub.1082847169 schema:CreativeWork
    463 https://doi.org/10.1002/ijc.24844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034369046
    464 rdf:type schema:CreativeWork
    465 https://doi.org/10.1016/0092-8674(90)90409-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040120250
    466 rdf:type schema:CreativeWork
    467 https://doi.org/10.1016/j.canlet.2011.02.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043991108
    468 rdf:type schema:CreativeWork
    469 https://doi.org/10.1016/j.ccr.2014.01.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002498840
    470 rdf:type schema:CreativeWork
    471 https://doi.org/10.1016/j.celrep.2014.08.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041908806
    472 rdf:type schema:CreativeWork
    473 https://doi.org/10.1016/j.chom.2014.02.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026644575
    474 rdf:type schema:CreativeWork
    475 https://doi.org/10.1016/j.chom.2014.02.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014449031
    476 rdf:type schema:CreativeWork
    477 https://doi.org/10.1016/j.ejogrb.2009.07.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049915789
    478 rdf:type schema:CreativeWork
    479 https://doi.org/10.1016/j.jhep.2006.05.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037562150
    480 rdf:type schema:CreativeWork
    481 https://doi.org/10.1016/j.molcel.2015.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022270616
    482 rdf:type schema:CreativeWork
    483 https://doi.org/10.1016/j.tig.2012.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009222806
    484 rdf:type schema:CreativeWork
    485 https://doi.org/10.1016/j.virol.2009.06.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041496962
    486 rdf:type schema:CreativeWork
    487 https://doi.org/10.1016/j.virol.2013.12.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020109637
    488 rdf:type schema:CreativeWork
    489 https://doi.org/10.1016/s0304-3835(01)00871-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037780457
    490 rdf:type schema:CreativeWork
    491 https://doi.org/10.1016/s1470-2045(09)70096-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009050725
    492 rdf:type schema:CreativeWork
    493 https://doi.org/10.1016/s1470-2045(12)70137-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037022536
    494 rdf:type schema:CreativeWork
    495 https://doi.org/10.1056/nejm199601113340213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016487843
    496 rdf:type schema:CreativeWork
    497 https://doi.org/10.1056/nejmoa021641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036284978
    498 rdf:type schema:CreativeWork
    499 https://doi.org/10.1073/pnas.0506580102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037705714
    500 rdf:type schema:CreativeWork
    501 https://doi.org/10.1091/mbc.02-02-0030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038504953
    502 rdf:type schema:CreativeWork
    503 https://doi.org/10.1091/mbc.02-02-0030. schema:sameAs https://app.dimensions.ai/details/publication/pub.1020036628
    504 rdf:type schema:CreativeWork
    505 https://doi.org/10.1093/bioinformatics/btp352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023014918
    506 rdf:type schema:CreativeWork
    507 https://doi.org/10.1093/bioinformatics/btp616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023247882
    508 rdf:type schema:CreativeWork
    509 https://doi.org/10.1093/bioinformatics/bts678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038581758
    510 rdf:type schema:CreativeWork
    511 https://doi.org/10.1093/nar/gkg786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000364966
    512 rdf:type schema:CreativeWork
    513 https://doi.org/10.1093/nar/gkt1196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015989191
    514 rdf:type schema:CreativeWork
    515 https://doi.org/10.1093/nar/gku1207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039030168
    516 rdf:type schema:CreativeWork
    517 https://doi.org/10.1101/gad.11.16.2090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026178926
    518 rdf:type schema:CreativeWork
    519 https://doi.org/10.1101/gr.135350.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012073457
    520 rdf:type schema:CreativeWork
    521 https://doi.org/10.1101/gr.161034.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018538260
    522 rdf:type schema:CreativeWork
    523 https://doi.org/10.1111/igc.0b013e3181af711e schema:sameAs https://app.dimensions.ai/details/publication/pub.1061838100
    524 rdf:type schema:CreativeWork
    525 https://doi.org/10.1126/science.1156725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062457660
    526 rdf:type schema:CreativeWork
    527 https://doi.org/10.1126/science.1254257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046815039
    528 rdf:type schema:CreativeWork
    529 https://doi.org/10.1128/jvi.01162-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027858553
    530 rdf:type schema:CreativeWork
    531 https://doi.org/10.1136/jclinpath-2014-202560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013869709
    532 rdf:type schema:CreativeWork
    533 https://doi.org/10.1158/1078-0432.ccr-05-2573 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012224313
    534 rdf:type schema:CreativeWork
    535 https://doi.org/10.1158/1078-0432.ccr-06-0571 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020795903
    536 rdf:type schema:CreativeWork
    537 https://doi.org/10.1158/1078-0432.ccr-14-0122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041393209
    538 rdf:type schema:CreativeWork
    539 https://doi.org/10.1289/ehp.95103s8259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064746153
    540 rdf:type schema:CreativeWork
    541 https://doi.org/10.1371/journal.pcbi.1002464 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050428866
    542 rdf:type schema:CreativeWork
    543 https://doi.org/10.1371/journal.pcbi.1003696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022849102
    544 rdf:type schema:CreativeWork
    545 https://doi.org/10.1371/journal.pone.0016266 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039493284
    546 rdf:type schema:CreativeWork
    547 https://doi.org/10.1371/journal.pone.0116151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020563858
    548 rdf:type schema:CreativeWork
    549 https://doi.org/10.1534/g3.113.005777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028964445
    550 rdf:type schema:CreativeWork
    551 https://doi.org/10.3892/ol.2013.1483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012344741
    552 rdf:type schema:CreativeWork
    553 https://doi.org/10.3892/or_00000729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071537513
    554 rdf:type schema:CreativeWork
    555 https://www.grid.ac/institutes/grid.1003.2 schema:alternateName University of Queensland
    556 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    557 Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia
    558 rdf:type schema:Organization
    559 https://www.grid.ac/institutes/grid.13402.34 schema:alternateName Zhejiang University
    560 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    561 James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China
    562 rdf:type schema:Organization
    563 https://www.grid.ac/institutes/grid.21155.32 schema:alternateName Beijing Genomics Institute
    564 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    565 The Guangdong Enterprise Key Laboratory of Human Disease Genomics, BGI-Shenzhen, 518083, Shenzhen, China
    566 rdf:type schema:Organization
    567 https://www.grid.ac/institutes/grid.263826.b schema:alternateName Southeast University
    568 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    569 School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, China
    570 State Key Laboratory of Bioelectronics, Southeast University, 210096, Nanjing, China
    571 rdf:type schema:Organization
    572 https://www.grid.ac/institutes/grid.410726.6 schema:alternateName University of Chinese Academy of Sciences
    573 schema:name BGI-Education Center, University of Chinese Academy of Sciences, 518083, Shenzhen, China
    574 BGI-Shenzhen, 518083, Shenzhen, China
    575 College of Life Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China
    576 rdf:type schema:Organization
    577 https://www.grid.ac/institutes/grid.48336.3a schema:alternateName National Cancer Institute
    578 schema:name Cancer and Inflammation Program, National Cancer Institute at Frederick, Building 560, 21702, Frederick, MD, USA
    579 rdf:type schema:Organization
    580 https://www.grid.ac/institutes/grid.5254.6 schema:alternateName University of Copenhagen
    581 schema:name BGI-Shenzhen, 518083, Shenzhen, China
    582 Department of Biology, University of Copenhagen, 1599, Copenhagen, Denmark
    583 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...