Basal body positioning and anchoring in the multiciliated cell Paramecium tetraurelia: roles of OFD1 and VFL3 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Hakim Bengueddach, Michel Lemullois, Anne Aubusson-Fleury, France Koll

ABSTRACT

BACKGROUND: The development of a ciliary axoneme requires the correct docking of the basal body at cytoplasmic vesicles or plasma membrane. In the multiciliated cell Paramecium, three conserved proteins, FOR20, Centrin 2, and Centrin 3 participate in this process, FOR20 and Centrin 2 being involved in the assembly of the transition zone. We investigated the function of two other evolutionary conserved proteins, OFD1 and VFL3, likely involved in this process. RESULTS: In Paramecium tetraurelia, a single gene encodes OFD1, while four genes encode four isoforms of VFL3, grouped into two families, VFL3-A and VFL3-B. Depletion of OFD1 and the sole VFL3-A family impairs basal body docking. Loss of OFD1 yields a defective assembly of the basal body distal part. Like FOR20, OFD1 is recruited early during basal body assembly and localizes at the transition zone between axoneme and membrane at the level of the microtubule doublets. While the recruitment of OFD1 and Centrin 2 proceed independently, the localizations of OFD1 and FOR20 at the basal body are interdependent. In contrast, in VFL3-A depleted cells, the unanchored basal bodies harbor a fully organized distal part but display an abnormal distribution of their associated rootlets which mark their rotational asymmetry. VFL3-A, which is required for the recruitment of Centrin 3, is transiently present near the basal bodies at an early step of their duplication. VFL3-A localizes at the junction between the striated rootlet and the basal body. CONCLUSION: Our results demonstrate the conserved role of OFD1 in the anchoring mechanisms of motile cilia and establish its relations with FOR20 and Centrin 2. They support the hypothesis of its association with microtubule doublets. They suggest that the primary defect of VFL3 depletion is a loss of the rotational asymmetry of the basal body which specifies the sites of assembly of the appendages which guide the movement of basal bodies toward the cell surface. The localization of VFL3 outside of the basal body suggests that extrinsic factors could control this asymmetry. More... »

PAGES

6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13630-017-0050-z

DOI

http://dx.doi.org/10.1186/s13630-017-0050-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084252552

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28367320


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris Sud, Universit\u00e9 Paris-Saclay, 1 Avenue de la Terrasse, 91198, Gif sur Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bengueddach", 
        "givenName": "Hakim", 
        "id": "sg:person.012301354463.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012301354463.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris Sud, Universit\u00e9 Paris-Saclay, 1 Avenue de la Terrasse, 91198, Gif sur Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lemullois", 
        "givenName": "Michel", 
        "id": "sg:person.01113703037.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113703037.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris Sud, Universit\u00e9 Paris-Saclay, 1 Avenue de la Terrasse, 91198, Gif sur Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aubusson-Fleury", 
        "givenName": "Anne", 
        "id": "sg:person.0611645106.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611645106.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris Sud, Universit\u00e9 Paris-Saclay, 1 Avenue de la Terrasse, 91198, Gif sur Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koll", 
        "givenName": "France", 
        "id": "sg:person.01272777106.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272777106.89"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/ng.104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001748482", 
          "https://doi.org/10.1038/ng.104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.15.2.363", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001806407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201406140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002786545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0091-679x(08)61758-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003400185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.153.1.63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004844669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.107.056812", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005492733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0932-4739(96)80076-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006767734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0052937", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007967601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0248-4900(03)00005-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008005441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2011.63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008071894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddn356", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008129230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.01120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011947636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.8.6.1063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012292492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-185x.1981.tb00350.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014532917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkn180", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014918820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(79)90473-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015606062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1684", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016249561", 
          "https://doi.org/10.1038/ng1684"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1684", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016249561", 
          "https://doi.org/10.1038/ng1684"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2005.11.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016435834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.114.163287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016879146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.114.163287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016879146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm3227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017418967", 
          "https://doi.org/10.1038/nrm3227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.065045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018557692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/27579", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023285321", 
          "https://doi.org/10.1038/27579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/27579", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023285321", 
          "https://doi.org/10.1038/27579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.61.2.461", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023817333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.473008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025371857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.protis.2013.04.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028159800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.108639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031128979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033286768", 
          "https://doi.org/10.1038/nature05230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033286768", 
          "https://doi.org/10.1038/nature05230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033286768", 
          "https://doi.org/10.1038/nature05230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkl777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034141539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2009.12.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034275515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/2046-2530-4-s1-p26", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034442261", 
          "https://doi.org/10.1186/2046-2530-4-s1-p26"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1995.1176", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034731798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(91)90330-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035134503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(01)02548-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038114959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.53.2.275", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038506360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2015.10.065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038517920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e14-02-0735", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039650221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.78.2.451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040107495"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0248-4900(94)80002-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044590488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.protis.2012.11.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044882041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201409123", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046967362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ceb.2015.04.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051291373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.96.6.1697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052035361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/318802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058622542"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddv488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059645739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/pdb.prot5365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060411238"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jmicro.a050974", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076725427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079412918", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080120128", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081249241", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "BACKGROUND: The development of a ciliary axoneme requires the correct docking of the basal body at cytoplasmic vesicles or plasma membrane. In the multiciliated cell Paramecium, three conserved proteins, FOR20, Centrin 2, and Centrin 3 participate in this process, FOR20 and Centrin 2 being involved in the assembly of the transition zone. We investigated the function of two other evolutionary conserved proteins, OFD1 and VFL3, likely involved in this process.\nRESULTS: In Paramecium tetraurelia, a single gene encodes OFD1, while four genes encode four isoforms of VFL3, grouped into two families, VFL3-A and VFL3-B. Depletion of OFD1 and the sole VFL3-A family impairs basal body docking. Loss of OFD1 yields a defective assembly of the basal body distal part. Like FOR20, OFD1 is recruited early during basal body assembly and localizes at the transition zone between axoneme and membrane at the level of the microtubule doublets. While the recruitment of OFD1 and Centrin 2 proceed independently, the localizations of OFD1 and FOR20 at the basal body are interdependent. In contrast, in VFL3-A depleted cells, the unanchored basal bodies harbor a fully organized distal part but display an abnormal distribution of their associated rootlets which mark their rotational asymmetry. VFL3-A, which is required for the recruitment of Centrin 3, is transiently present near the basal bodies at an early step of their duplication. VFL3-A localizes at the junction between the striated rootlet and the basal body.\nCONCLUSION: Our results demonstrate the conserved role of OFD1 in the anchoring mechanisms of motile cilia and establish its relations with FOR20 and Centrin 2. They support the hypothesis of its association with microtubule doublets. They suggest that the primary defect of VFL3 depletion is a loss of the rotational asymmetry of the basal body which specifies the sites of assembly of the appendages which guide the movement of basal bodies toward the cell surface. The localization of VFL3 outside of the basal body suggests that extrinsic factors could control this asymmetry.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13630-017-0050-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1046983", 
        "issn": [
          "2046-2530"
        ], 
        "name": "Cilia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "Basal body positioning and anchoring in the multiciliated cell Paramecium tetraurelia: roles of OFD1 and VFL3", 
    "pagination": "6", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b113d84b8de42b5abd9fc20cd27bd6ba2eaddb781cde50978ef7fe36ab607a0c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28367320"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101585639"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13630-017-0050-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084252552"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13630-017-0050-z", 
      "https://app.dimensions.ai/details/publication/pub.1084252552"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000348_0000000348/records_54325_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13630-017-0050-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13630-017-0050-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13630-017-0050-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13630-017-0050-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13630-017-0050-z'


 

This table displays all metadata directly associated to this object as RDF triples.

239 TRIPLES      21 PREDICATES      78 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13630-017-0050-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N25d731c737ee4fd58d899464cd48a210
4 schema:citation sg:pub.10.1038/27579
5 sg:pub.10.1038/nature05230
6 sg:pub.10.1038/ng.104
7 sg:pub.10.1038/ng1684
8 sg:pub.10.1038/nrm3227
9 sg:pub.10.1186/2046-2530-4-s1-p26
10 https://app.dimensions.ai/details/publication/pub.1079412918
11 https://app.dimensions.ai/details/publication/pub.1080120128
12 https://app.dimensions.ai/details/publication/pub.1081249241
13 https://doi.org/10.1006/dbio.1995.1176
14 https://doi.org/10.1016/0012-1606(91)90330-6
15 https://doi.org/10.1016/0014-4827(79)90473-7
16 https://doi.org/10.1016/j.ceb.2015.04.015
17 https://doi.org/10.1016/j.cell.2015.10.065
18 https://doi.org/10.1016/j.cub.2005.11.038
19 https://doi.org/10.1016/j.devcel.2009.12.022
20 https://doi.org/10.1016/j.protis.2012.11.003
21 https://doi.org/10.1016/j.protis.2013.04.003
22 https://doi.org/10.1016/s0091-679x(08)61758-6
23 https://doi.org/10.1016/s0168-9525(01)02548-3
24 https://doi.org/10.1016/s0248-4900(03)00005-4
25 https://doi.org/10.1016/s0248-4900(94)80002-2
26 https://doi.org/10.1016/s0932-4739(96)80076-9
27 https://doi.org/10.1038/emboj.2011.63
28 https://doi.org/10.1073/pnas.53.2.275
29 https://doi.org/10.1073/pnas.61.2.461
30 https://doi.org/10.1083/jcb.15.2.363
31 https://doi.org/10.1083/jcb.153.1.63
32 https://doi.org/10.1083/jcb.201406140
33 https://doi.org/10.1083/jcb.201409123
34 https://doi.org/10.1083/jcb.78.2.451
35 https://doi.org/10.1083/jcb.96.6.1697
36 https://doi.org/10.1086/318802
37 https://doi.org/10.1091/mbc.8.6.1063
38 https://doi.org/10.1091/mbc.e14-02-0735
39 https://doi.org/10.1093/hmg/ddn356
40 https://doi.org/10.1093/hmg/ddv488
41 https://doi.org/10.1093/nar/gkl777
42 https://doi.org/10.1093/nar/gkn180
43 https://doi.org/10.1093/oxfordjournals.jmicro.a050974
44 https://doi.org/10.1101/gad.473008
45 https://doi.org/10.1101/pdb.prot5365
46 https://doi.org/10.1105/tpc.107.056812
47 https://doi.org/10.1111/j.1469-185x.1981.tb00350.x
48 https://doi.org/10.1242/jcs.01120
49 https://doi.org/10.1242/jcs.065045
50 https://doi.org/10.1242/jcs.108639
51 https://doi.org/10.1371/journal.pone.0052937
52 https://doi.org/10.1534/genetics.114.163287
53 schema:datePublished 2017-12
54 schema:datePublishedReg 2017-12-01
55 schema:description BACKGROUND: The development of a ciliary axoneme requires the correct docking of the basal body at cytoplasmic vesicles or plasma membrane. In the multiciliated cell Paramecium, three conserved proteins, FOR20, Centrin 2, and Centrin 3 participate in this process, FOR20 and Centrin 2 being involved in the assembly of the transition zone. We investigated the function of two other evolutionary conserved proteins, OFD1 and VFL3, likely involved in this process. RESULTS: In Paramecium tetraurelia, a single gene encodes OFD1, while four genes encode four isoforms of VFL3, grouped into two families, VFL3-A and VFL3-B. Depletion of OFD1 and the sole VFL3-A family impairs basal body docking. Loss of OFD1 yields a defective assembly of the basal body distal part. Like FOR20, OFD1 is recruited early during basal body assembly and localizes at the transition zone between axoneme and membrane at the level of the microtubule doublets. While the recruitment of OFD1 and Centrin 2 proceed independently, the localizations of OFD1 and FOR20 at the basal body are interdependent. In contrast, in VFL3-A depleted cells, the unanchored basal bodies harbor a fully organized distal part but display an abnormal distribution of their associated rootlets which mark their rotational asymmetry. VFL3-A, which is required for the recruitment of Centrin 3, is transiently present near the basal bodies at an early step of their duplication. VFL3-A localizes at the junction between the striated rootlet and the basal body. CONCLUSION: Our results demonstrate the conserved role of OFD1 in the anchoring mechanisms of motile cilia and establish its relations with FOR20 and Centrin 2. They support the hypothesis of its association with microtubule doublets. They suggest that the primary defect of VFL3 depletion is a loss of the rotational asymmetry of the basal body which specifies the sites of assembly of the appendages which guide the movement of basal bodies toward the cell surface. The localization of VFL3 outside of the basal body suggests that extrinsic factors could control this asymmetry.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf Nb434427d82d64c9c8e21708ed7a67552
60 Ndc250841072841608edb74ba296064e7
61 sg:journal.1046983
62 schema:name Basal body positioning and anchoring in the multiciliated cell Paramecium tetraurelia: roles of OFD1 and VFL3
63 schema:pagination 6
64 schema:productId N58bdde506a2c4cbeb2c03c0666184e8b
65 N660d5fcf33b1436abb4f029fcd99263e
66 N9b56f6f4353a45038c9a5c1ca0930cce
67 Na0677eac35f04d318925ddc53486e256
68 Nbf6dff3befb24e839c90a7ec0fb686bc
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084252552
70 https://doi.org/10.1186/s13630-017-0050-z
71 schema:sdDatePublished 2019-04-11T10:19
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N11fa10c0a4b34360a5dd46d5d17e73cb
74 schema:url https://link.springer.com/10.1186%2Fs13630-017-0050-z
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N11fa10c0a4b34360a5dd46d5d17e73cb schema:name Springer Nature - SN SciGraph project
79 rdf:type schema:Organization
80 N25d731c737ee4fd58d899464cd48a210 rdf:first sg:person.012301354463.20
81 rdf:rest Naa847771d0644caa98aa7687e83a8722
82 N344099515e39456dbe254582e31c02df rdf:first sg:person.0611645106.63
83 rdf:rest N7decd2bf1eb745e8bb63e91e6637572e
84 N58bdde506a2c4cbeb2c03c0666184e8b schema:name dimensions_id
85 schema:value pub.1084252552
86 rdf:type schema:PropertyValue
87 N660d5fcf33b1436abb4f029fcd99263e schema:name nlm_unique_id
88 schema:value 101585639
89 rdf:type schema:PropertyValue
90 N7decd2bf1eb745e8bb63e91e6637572e rdf:first sg:person.01272777106.89
91 rdf:rest rdf:nil
92 N9b56f6f4353a45038c9a5c1ca0930cce schema:name doi
93 schema:value 10.1186/s13630-017-0050-z
94 rdf:type schema:PropertyValue
95 Na0677eac35f04d318925ddc53486e256 schema:name readcube_id
96 schema:value b113d84b8de42b5abd9fc20cd27bd6ba2eaddb781cde50978ef7fe36ab607a0c
97 rdf:type schema:PropertyValue
98 Naa847771d0644caa98aa7687e83a8722 rdf:first sg:person.01113703037.95
99 rdf:rest N344099515e39456dbe254582e31c02df
100 Nb434427d82d64c9c8e21708ed7a67552 schema:issueNumber 1
101 rdf:type schema:PublicationIssue
102 Nbf6dff3befb24e839c90a7ec0fb686bc schema:name pubmed_id
103 schema:value 28367320
104 rdf:type schema:PropertyValue
105 Ndc250841072841608edb74ba296064e7 schema:volumeNumber 6
106 rdf:type schema:PublicationVolume
107 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
108 schema:name Biological Sciences
109 rdf:type schema:DefinedTerm
110 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
111 schema:name Biochemistry and Cell Biology
112 rdf:type schema:DefinedTerm
113 sg:journal.1046983 schema:issn 2046-2530
114 schema:name Cilia
115 rdf:type schema:Periodical
116 sg:person.01113703037.95 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
117 schema:familyName Lemullois
118 schema:givenName Michel
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113703037.95
120 rdf:type schema:Person
121 sg:person.012301354463.20 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
122 schema:familyName Bengueddach
123 schema:givenName Hakim
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012301354463.20
125 rdf:type schema:Person
126 sg:person.01272777106.89 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
127 schema:familyName Koll
128 schema:givenName France
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272777106.89
130 rdf:type schema:Person
131 sg:person.0611645106.63 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
132 schema:familyName Aubusson-Fleury
133 schema:givenName Anne
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611645106.63
135 rdf:type schema:Person
136 sg:pub.10.1038/27579 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023285321
137 https://doi.org/10.1038/27579
138 rdf:type schema:CreativeWork
139 sg:pub.10.1038/nature05230 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033286768
140 https://doi.org/10.1038/nature05230
141 rdf:type schema:CreativeWork
142 sg:pub.10.1038/ng.104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001748482
143 https://doi.org/10.1038/ng.104
144 rdf:type schema:CreativeWork
145 sg:pub.10.1038/ng1684 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016249561
146 https://doi.org/10.1038/ng1684
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/nrm3227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017418967
149 https://doi.org/10.1038/nrm3227
150 rdf:type schema:CreativeWork
151 sg:pub.10.1186/2046-2530-4-s1-p26 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034442261
152 https://doi.org/10.1186/2046-2530-4-s1-p26
153 rdf:type schema:CreativeWork
154 https://app.dimensions.ai/details/publication/pub.1079412918 schema:CreativeWork
155 https://app.dimensions.ai/details/publication/pub.1080120128 schema:CreativeWork
156 https://app.dimensions.ai/details/publication/pub.1081249241 schema:CreativeWork
157 https://doi.org/10.1006/dbio.1995.1176 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034731798
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/0012-1606(91)90330-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035134503
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/0014-4827(79)90473-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015606062
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.ceb.2015.04.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051291373
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/j.cell.2015.10.065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038517920
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1016/j.cub.2005.11.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016435834
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/j.devcel.2009.12.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034275515
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1016/j.protis.2012.11.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044882041
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1016/j.protis.2013.04.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028159800
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/s0091-679x(08)61758-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003400185
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/s0168-9525(01)02548-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038114959
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/s0248-4900(03)00005-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008005441
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/s0248-4900(94)80002-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044590488
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/s0932-4739(96)80076-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006767734
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1038/emboj.2011.63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008071894
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1073/pnas.53.2.275 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038506360
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1073/pnas.61.2.461 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023817333
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1083/jcb.15.2.363 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001806407
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1083/jcb.153.1.63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004844669
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1083/jcb.201406140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002786545
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1083/jcb.201409123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046967362
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1083/jcb.78.2.451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040107495
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1083/jcb.96.6.1697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052035361
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1086/318802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058622542
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1091/mbc.8.6.1063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012292492
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1091/mbc.e14-02-0735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039650221
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1093/hmg/ddn356 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008129230
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1093/hmg/ddv488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059645739
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1093/nar/gkl777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034141539
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1093/nar/gkn180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014918820
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1093/oxfordjournals.jmicro.a050974 schema:sameAs https://app.dimensions.ai/details/publication/pub.1076725427
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1101/gad.473008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025371857
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1101/pdb.prot5365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060411238
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1105/tpc.107.056812 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005492733
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1111/j.1469-185x.1981.tb00350.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014532917
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1242/jcs.01120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011947636
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1242/jcs.065045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018557692
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1242/jcs.108639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031128979
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1371/journal.pone.0052937 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007967601
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1534/genetics.114.163287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016879146
236 rdf:type schema:CreativeWork
237 https://www.grid.ac/institutes/grid.462411.4 schema:alternateName Institute of Integrative Biology of the Cell
238 schema:name Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris Sud, Université Paris-Saclay, 1 Avenue de la Terrasse, 91198, Gif sur Yvette, France
239 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...