Modulation of lipolysis and glycolysis pathways in cancer stem cells changed multipotentiality and differentiation capacity toward endothelial lineage View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Ayda Pouyafar, Milad Zadi Heydarabad, Jalal Abdolali Zade, Reza Rahbarghazi, Mehdi Talebi

ABSTRACT

Cancer stem cells obtain energy demand through the activation of glycolysis and lipolysis. It seems that the use of approached targeting glycolysis and lipolysis could be an effective strategy for the inhibition of cancer stem cells. In the current experiment, we studied the potential effect of glycolysis and lipolysis inhibition on cancer stem cells differentiation and mesenchymal-epithelial-transition capacity. Cancer stem cells were enriched from human ovarian cells namely SKOV3 by using MACS technique. Cells were exposed to Lonidamine, an inhibitor of glycolysis, and TOFA, a potent inhibitor of lipolysis for 7 days in endothelial differentiation medium; EGM-2 and cell viability was studied by MTT assay. At the respective time point, the transcription level of genes participating in EMT such as Zeb-1, -2, Vimentin, Snail-1, -2 and VE-cadherin were measured by real-time PCR analysis. Our data noted that the inhibition of lipolysis and glycolysis could decrease cell viability compared to the control of cancer stem cells. The inhibition of glycolysis prohibited the expression of Zeb-1, Snails, and Vimentin while increased endothelial differentiation rate indicated by the expression of VE-cadherin. In contrast, the inhibition of lipolysis increased EMT associated genes and reduced endothelial differentiation rate by suppressing the transcription of VE-cadherin. Notably, the simultaneous inhibition of glycolysis and lipolysis had moderate effects on the transcription of EMT genes. We concluded that the modulation of the metabolic pathway of glycolysis in ovarian CSCs is more effective than the inhibition of lipolysis in the control of angiogenesis potential and stemness feature. More... »

PAGES

30

Journal

TITLE

Cell & Bioscience

ISSUE

1

VOLUME

9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13578-019-0293-z

DOI

http://dx.doi.org/10.1186/s13578-019-0293-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113045260

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30962872


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Tabriz University of Medical Sciences", 
          "id": "https://www.grid.ac/institutes/grid.412888.f", 
          "name": [
            "Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Daneshgah St., 5166614756, Tabriz, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pouyafar", 
        "givenName": "Ayda", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tabriz University of Medical Sciences", 
          "id": "https://www.grid.ac/institutes/grid.412888.f", 
          "name": [
            "Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Daneshgah St., 5166614756, Tabriz, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heydarabad", 
        "givenName": "Milad Zadi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tabriz University of Medical Sciences", 
          "id": "https://www.grid.ac/institutes/grid.412888.f", 
          "name": [
            "Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zade", 
        "givenName": "Jalal Abdolali", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tabriz University of Medical Sciences", 
          "id": "https://www.grid.ac/institutes/grid.412888.f", 
          "name": [
            "Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran", 
            "Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rahbarghazi", 
        "givenName": "Reza", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tabriz University of Medical Sciences", 
          "id": "https://www.grid.ac/institutes/grid.412888.f", 
          "name": [
            "Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Talebi", 
        "givenName": "Mehdi", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.pharmthera.2013.01.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004970932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.2059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018284162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12943-016-0555-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024943407", 
          "https://doi.org/10.1186/s12943-016-0555-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12943-016-0555-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024943407", 
          "https://doi.org/10.1186/s12943-016-0555-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci39104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026879393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci39104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026879393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.24671", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045028048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2013.02.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045945946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jcmm.13126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084214345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-02256-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085530529", 
          "https://doi.org/10.1038/s41598-017-02256-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13046-018-0784-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104600058", 
          "https://doi.org/10.1186/s13046-018-0784-5"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Cancer stem cells obtain energy demand through the activation of glycolysis and lipolysis. It seems that the use of approached targeting glycolysis and lipolysis could be an effective strategy for the inhibition of cancer stem cells. In the current experiment, we studied the potential effect of glycolysis and lipolysis inhibition on cancer stem cells differentiation and mesenchymal-epithelial-transition capacity. Cancer stem cells were enriched from human ovarian cells namely SKOV3 by using MACS technique. Cells were exposed to Lonidamine, an inhibitor of glycolysis, and TOFA, a potent inhibitor of lipolysis for 7\u00a0days in endothelial differentiation medium; EGM-2 and cell viability was studied by MTT assay. At the respective time point, the transcription level of genes participating in EMT such as Zeb-1, -2, Vimentin, Snail-1, -2 and VE-cadherin were measured by real-time PCR analysis. Our data noted that the inhibition of lipolysis and glycolysis could decrease cell viability compared to the control of cancer stem cells. The inhibition of glycolysis prohibited the expression of Zeb-1, Snails, and Vimentin while increased endothelial differentiation rate indicated by the expression of VE-cadherin. In contrast, the inhibition of lipolysis increased EMT associated genes and reduced endothelial differentiation rate by suppressing the transcription of VE-cadherin. Notably, the simultaneous inhibition of glycolysis and lipolysis had moderate effects on the transcription of EMT genes. We concluded that the modulation of the metabolic pathway of glycolysis in ovarian CSCs is more effective than the inhibition of lipolysis in the control of angiogenesis potential and stemness feature.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13578-019-0293-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045176", 
        "issn": [
          "2045-3701"
        ], 
        "name": "Cell & Bioscience", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Modulation of lipolysis and glycolysis pathways in cancer stem cells changed multipotentiality and differentiation capacity toward endothelial lineage", 
    "pagination": "30", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13578-019-0293-z"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7f6864903933e522e77dac3a5050f56b04a166332eef596bc3d19b9fcbff50ac"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1113045260"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101561195"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30962872"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13578-019-0293-z", 
      "https://app.dimensions.ai/details/publication/pub.1113045260"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56176_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13578-019-0293-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0293-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0293-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0293-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0293-z'


 

This table displays all metadata directly associated to this object as RDF triples.

123 TRIPLES      21 PREDICATES      38 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13578-019-0293-z schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N6459a9c7781e4c11a3565f9cd0d23200
4 schema:citation sg:pub.10.1038/s41598-017-02256-4
5 sg:pub.10.1186/s12943-016-0555-x
6 sg:pub.10.1186/s13046-018-0784-5
7 https://doi.org/10.1002/jcb.24671
8 https://doi.org/10.1016/j.ccr.2013.02.021
9 https://doi.org/10.1016/j.pharmthera.2013.01.014
10 https://doi.org/10.1111/jcmm.13126
11 https://doi.org/10.1172/jci39104
12 https://doi.org/10.18632/oncotarget.2059
13 schema:datePublished 2019-12
14 schema:datePublishedReg 2019-12-01
15 schema:description Cancer stem cells obtain energy demand through the activation of glycolysis and lipolysis. It seems that the use of approached targeting glycolysis and lipolysis could be an effective strategy for the inhibition of cancer stem cells. In the current experiment, we studied the potential effect of glycolysis and lipolysis inhibition on cancer stem cells differentiation and mesenchymal-epithelial-transition capacity. Cancer stem cells were enriched from human ovarian cells namely SKOV3 by using MACS technique. Cells were exposed to Lonidamine, an inhibitor of glycolysis, and TOFA, a potent inhibitor of lipolysis for 7 days in endothelial differentiation medium; EGM-2 and cell viability was studied by MTT assay. At the respective time point, the transcription level of genes participating in EMT such as <i>Zeb</i>-1, -2, <i>Vimentin</i>, <i>Snail</i>-1, -2 and <i>VE</i>-<i>cadherin</i> were measured by real-time PCR analysis. Our data noted that the inhibition of lipolysis and glycolysis could decrease cell viability compared to the control of cancer stem cells. The inhibition of glycolysis prohibited the expression of <i>Zeb</i>-1, <i>Snails</i>, and <i>Vimentin</i> while increased endothelial differentiation rate indicated by the expression of <i>VE</i>-<i>cadherin</i>. In contrast, the inhibition of lipolysis increased EMT associated genes and reduced endothelial differentiation rate by suppressing the transcription of <i>VE</i>-<i>cadherin</i>. Notably, the simultaneous inhibition of glycolysis and lipolysis had moderate effects on the transcription of EMT genes. We concluded that the modulation of the metabolic pathway of glycolysis in ovarian CSCs is more effective than the inhibition of lipolysis in the control of angiogenesis potential and stemness feature.
16 schema:genre research_article
17 schema:inLanguage en
18 schema:isAccessibleForFree true
19 schema:isPartOf N4188b7555ff7489ab998edd1803a1963
20 N56dc889fbce14af98877c680608a3f23
21 sg:journal.1045176
22 schema:name Modulation of lipolysis and glycolysis pathways in cancer stem cells changed multipotentiality and differentiation capacity toward endothelial lineage
23 schema:pagination 30
24 schema:productId N0e21e2eac1cb4c0597f3fd0f148c8d45
25 N24517804f5dc45ebba02386120402860
26 N5adc972813464cbcb1749b118444abab
27 N5bf94d2ec4d947ca8a96e5bf9d127f8a
28 Na9fda330bb3949b5a9bbc32193046c71
29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113045260
30 https://doi.org/10.1186/s13578-019-0293-z
31 schema:sdDatePublished 2019-04-15T09:19
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher Nb9b7e9d8711f441db36e206699baeece
34 schema:url https://link.springer.com/10.1186%2Fs13578-019-0293-z
35 sgo:license sg:explorer/license/
36 sgo:sdDataset articles
37 rdf:type schema:ScholarlyArticle
38 N0e21e2eac1cb4c0597f3fd0f148c8d45 schema:name doi
39 schema:value 10.1186/s13578-019-0293-z
40 rdf:type schema:PropertyValue
41 N2220d7ad9136495ea71f5aac0a0443b2 rdf:first Nc842992f4d8d4f78b960c7124147fb27
42 rdf:rest Nee641da5869e46689259042c7f9a725d
43 N24517804f5dc45ebba02386120402860 schema:name dimensions_id
44 schema:value pub.1113045260
45 rdf:type schema:PropertyValue
46 N4188b7555ff7489ab998edd1803a1963 schema:volumeNumber 9
47 rdf:type schema:PublicationVolume
48 N56dc889fbce14af98877c680608a3f23 schema:issueNumber 1
49 rdf:type schema:PublicationIssue
50 N5adc972813464cbcb1749b118444abab schema:name pubmed_id
51 schema:value 30962872
52 rdf:type schema:PropertyValue
53 N5bf94d2ec4d947ca8a96e5bf9d127f8a schema:name nlm_unique_id
54 schema:value 101561195
55 rdf:type schema:PropertyValue
56 N6459a9c7781e4c11a3565f9cd0d23200 rdf:first N8a2c5ab46529426ab8d5ac753fb46a5b
57 rdf:rest Nc55f703403fc49e2991bf404d06b8250
58 N8a2c5ab46529426ab8d5ac753fb46a5b schema:affiliation https://www.grid.ac/institutes/grid.412888.f
59 schema:familyName Pouyafar
60 schema:givenName Ayda
61 rdf:type schema:Person
62 N9b61282480df418393ead35ee0c03c97 schema:affiliation https://www.grid.ac/institutes/grid.412888.f
63 schema:familyName Heydarabad
64 schema:givenName Milad Zadi
65 rdf:type schema:Person
66 Na9fda330bb3949b5a9bbc32193046c71 schema:name readcube_id
67 schema:value 7f6864903933e522e77dac3a5050f56b04a166332eef596bc3d19b9fcbff50ac
68 rdf:type schema:PropertyValue
69 Naac1c75e87254b89a0736e746e099101 rdf:first Ne131ca6287f749ef86283d677a1d3523
70 rdf:rest rdf:nil
71 Nb7e3f6bd8b5247e1a857f95794c6f6e6 schema:affiliation https://www.grid.ac/institutes/grid.412888.f
72 schema:familyName Rahbarghazi
73 schema:givenName Reza
74 rdf:type schema:Person
75 Nb9b7e9d8711f441db36e206699baeece schema:name Springer Nature - SN SciGraph project
76 rdf:type schema:Organization
77 Nc55f703403fc49e2991bf404d06b8250 rdf:first N9b61282480df418393ead35ee0c03c97
78 rdf:rest N2220d7ad9136495ea71f5aac0a0443b2
79 Nc842992f4d8d4f78b960c7124147fb27 schema:affiliation https://www.grid.ac/institutes/grid.412888.f
80 schema:familyName Zade
81 schema:givenName Jalal Abdolali
82 rdf:type schema:Person
83 Ne131ca6287f749ef86283d677a1d3523 schema:affiliation https://www.grid.ac/institutes/grid.412888.f
84 schema:familyName Talebi
85 schema:givenName Mehdi
86 rdf:type schema:Person
87 Nee641da5869e46689259042c7f9a725d rdf:first Nb7e3f6bd8b5247e1a857f95794c6f6e6
88 rdf:rest Naac1c75e87254b89a0736e746e099101
89 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
90 schema:name Biological Sciences
91 rdf:type schema:DefinedTerm
92 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
93 schema:name Genetics
94 rdf:type schema:DefinedTerm
95 sg:journal.1045176 schema:issn 2045-3701
96 schema:name Cell & Bioscience
97 rdf:type schema:Periodical
98 sg:pub.10.1038/s41598-017-02256-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085530529
99 https://doi.org/10.1038/s41598-017-02256-4
100 rdf:type schema:CreativeWork
101 sg:pub.10.1186/s12943-016-0555-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024943407
102 https://doi.org/10.1186/s12943-016-0555-x
103 rdf:type schema:CreativeWork
104 sg:pub.10.1186/s13046-018-0784-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104600058
105 https://doi.org/10.1186/s13046-018-0784-5
106 rdf:type schema:CreativeWork
107 https://doi.org/10.1002/jcb.24671 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045028048
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1016/j.ccr.2013.02.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045945946
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/j.pharmthera.2013.01.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004970932
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1111/jcmm.13126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084214345
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1172/jci39104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026879393
116 rdf:type schema:CreativeWork
117 https://doi.org/10.18632/oncotarget.2059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018284162
118 rdf:type schema:CreativeWork
119 https://www.grid.ac/institutes/grid.412888.f schema:alternateName Tabriz University of Medical Sciences
120 schema:name Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
121 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
122 Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Daneshgah St., 5166614756, Tabriz, Iran
123 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...