Ontology type: schema:ScholarlyArticle Open Access: True
2019-12
AUTHORSHuaming Cao, Dongsheng Yu, Xueyun Yan, Bing Wang, Zhiming Yu, Yu Song, Liang Sheng
ABSTRACTBackground: Endothelial cells (EC) are sensitive to changes in the microenvironment, including hypoxia and ischemia. Disruption of the microtubular network has been reported in cases of ischemia. However, the signaling pathways involved in hypoxia-induced microtubular disruption are unknown. The purpose of this study was to investigate the molecular mechanisms involved in hypoxia-induced microtubular disassembly in human umbilical vein endothelial cells (HUVECs). Results: HUVECs were cultured under normoxic or hypoxic conditions and pretreated with or without colchicine or paclitaxel. The MTT assay, Transwell assay, trans-endothelial permeability assay, and 5-bromo-2'-deoxy-uridine staining were used to test the survival rate, migration, permeability, and proliferation of cells, respectively. Transmission electron microscopy and phalloidin staining were used to observe the microstructure and polymerization of microtubules. The results show that the functions of HUVECs and the microtubular structure were destroyed by hypoxia, but were protected by paclitaxel and a reactive oxygen species (ROS) inhibitor. We further used western blot, a luciferase assay, and co-immunoprecipitation to describe a non-transcription-independent mechanism for PI3K activation-inhibited microtubular stability mediated by Stathmin1, a PI3K interactor that functions in microtubule depolymerization. Finally, we determined that hypoxia and ROS blocked the interaction between PI3K and Stathmin1 to activate disassembly of microtubules. Conclusion: Thus, our data demonstrate that hypoxia induced the production of ROS and damaged EC function by destroying the microtubular structure through the PI3K/stathmin1 pathway. More... »
PAGES20
http://scigraph.springernature.com/pub.10.1186/s13578-019-0283-1
DOIhttp://dx.doi.org/10.1186/s13578-019-0283-1
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1112220519
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30820314
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"name": [
"Department of Cardiology, Shibei Hospital, 4500 Gong He Xin Road, 200435, Shanghai, China"
],
"type": "Organization"
},
"familyName": "Cao",
"givenName": "Huaming",
"type": "Person"
},
{
"affiliation": {
"alternateName": "First Affiliated Hospital of Zhengzhou University",
"id": "https://www.grid.ac/institutes/grid.412633.1",
"name": [
"Department of Traditional Chinese Medicine, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, Henan, China",
"Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China",
"Henan Key Laboratory of Digestive Organ Transplantation, 450052, Zhengzhou, China"
],
"type": "Organization"
},
"familyName": "Yu",
"givenName": "Dongsheng",
"type": "Person"
},
{
"affiliation": {
"name": [
"Department of Cardiology, Shibei Hospital, 4500 Gong He Xin Road, 200435, Shanghai, China"
],
"type": "Organization"
},
"familyName": "Yan",
"givenName": "Xueyun",
"type": "Person"
},
{
"affiliation": {
"name": [
"Department of Cardiology, Shibei Hospital, 4500 Gong He Xin Road, 200435, Shanghai, China"
],
"type": "Organization"
},
"familyName": "Wang",
"givenName": "Bing",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Wuxi People's Hospital",
"id": "https://www.grid.ac/institutes/grid.460176.2",
"name": [
"Department of Cardiology, Wuxi People\u2019s Hospital Affiliated to Nanjing Medical University, 214023, Wuxi, Jiangsu, China"
],
"type": "Organization"
},
"familyName": "Yu",
"givenName": "Zhiming",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Xinxiang Medical University",
"id": "https://www.grid.ac/institutes/grid.412990.7",
"name": [
"Pharmacy College, Xinxiang Medical University, 601 Jinsui Avenue, Hongqi District, 453000, Xinxiang, Henan, China"
],
"type": "Organization"
},
"familyName": "Song",
"givenName": "Yu",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Xinxiang Medical University",
"id": "https://www.grid.ac/institutes/grid.412990.7",
"name": [
"Pharmacy College, Xinxiang Medical University, 601 Jinsui Avenue, Hongqi District, 453000, Xinxiang, Henan, China"
],
"type": "Organization"
},
"familyName": "Sheng",
"givenName": "Liang",
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/j.cellsig.2005.05.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004461990"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cellsig.2005.05.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004461990"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/0008-5472.can-05-2612",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005849810"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1096/fj.04-2328com",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006139532"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/1535-7163.mct-13-0401",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008335482"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1124/jpet.111.180992",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014561396"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/cas.12417",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015113845"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0008866",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015766097"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrm3227",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017418967",
"https://doi.org/10.1038/nrm3227"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1165/rcmb.2012-0161oc",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020956967"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1038/emboj.2012.273",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021453058"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3109/10715762.2013.867484",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022447543"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.18632/oncoscience.169",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023845611"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jcp.20359",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027451982"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m900343200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029543700"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature06892",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029723170",
"https://doi.org/10.1038/nature06892"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12013-016-0723-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033167314",
"https://doi.org/10.1007/s12013-016-0723-z"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11010-011-1013-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033451226",
"https://doi.org/10.1007/s11010-011-1013-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s13277-014-2629-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035606253",
"https://doi.org/10.1007/s13277-014-2629-y"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ygyno.2011.05.021",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036208224"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrd4623",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039979649",
"https://doi.org/10.1038/nrd4623"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1177/1947601911423654",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042073119"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1177/1947601911423654",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042073119"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2014.05.021",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042620142"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/01.cir.0000131515.03336.f8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043584862"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.lfs.2014.11.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043795867"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11010-011-1056-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048762584",
"https://doi.org/10.1007/s11010-011-1056-2"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.vph.2015.05.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050462559"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12192-011-0309-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050794928",
"https://doi.org/10.1007/s12192-011-0309-z"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/eurheartj/ehv428",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052997135"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m116.726083",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1058214905"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/1389201015666141111114442",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069176920"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms15289",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1085176440",
"https://doi.org/10.1038/ncomms15289"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1085374537",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.15252/embj.201696150",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1086109514"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.00749-17",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090908326"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.00749-17",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090908326"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3892/ijo.2017.4085",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090950265"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/jdi.12767",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092416724"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-12",
"datePublishedReg": "2019-12-01",
"description": "Background: Endothelial cells (EC) are sensitive to changes in the microenvironment, including hypoxia and ischemia. Disruption of the microtubular network has been reported in cases of ischemia. However, the signaling pathways involved in hypoxia-induced microtubular disruption are unknown. The purpose of this study was to investigate the molecular mechanisms involved in hypoxia-induced microtubular disassembly in human umbilical vein endothelial cells (HUVECs).\nResults: HUVECs were cultured under normoxic or hypoxic conditions and pretreated with or without colchicine or paclitaxel. The MTT assay, Transwell assay, trans-endothelial permeability assay, and 5-bromo-2'-deoxy-uridine staining were used to test the survival rate, migration, permeability, and proliferation of cells, respectively. Transmission electron microscopy and phalloidin staining were used to observe the microstructure and polymerization of microtubules. The results show that the functions of HUVECs and the microtubular structure were destroyed by hypoxia, but were protected by paclitaxel and a reactive oxygen species (ROS) inhibitor. We further used western blot, a luciferase assay, and co-immunoprecipitation to describe a non-transcription-independent mechanism for PI3K activation-inhibited microtubular stability mediated by Stathmin1, a PI3K interactor that functions in microtubule depolymerization. Finally, we determined that hypoxia and ROS blocked the interaction between PI3K and Stathmin1 to activate disassembly of microtubules.\nConclusion: Thus, our data demonstrate that hypoxia induced the production of ROS and damaged EC function by destroying the microtubular structure through the PI3K/stathmin1 pathway.",
"genre": "research_article",
"id": "sg:pub.10.1186/s13578-019-0283-1",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1045176",
"issn": [
"2045-3701"
],
"name": "Cell & Bioscience",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"name": "Hypoxia destroys the microstructure of microtubules and causes dysfunction of endothelial cells via the PI3K/Stathmin1 pathway",
"pagination": "20",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"e606fb39172bc54b40831769617e9eb6b2c2b1de33213725289ad40108175328"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30820314"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101561195"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/s13578-019-0283-1"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1112220519"
]
}
],
"sameAs": [
"https://doi.org/10.1186/s13578-019-0283-1",
"https://app.dimensions.ai/details/publication/pub.1112220519"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T11:18",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000354_0000000354/records_11704_00000002.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1186%2Fs13578-019-0283-1"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0283-1'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0283-1'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0283-1'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13578-019-0283-1'
This table displays all metadata directly associated to this object as RDF triples.
233 TRIPLES
21 PREDICATES
65 URIs
21 LITERALS
9 BLANK NODES