Dynamics expression of DmFKBP12/Calstabin during embryonic early development of Drosophila melanogaster View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Rui Feng, Xin Zhou, Wei Zhang, Tao Pu, Yuting Sun, Rong Yang, Dan Wang, Xiaofei Zhang, Yingfeng Gao, Zhenlu Cai, Yu Liang, Qiuxia Yu, Yajun Wu, Xinjuan Lei, Zhijia Liang, Odell Jones, Liyang Wang, Mengmeng Xu, Yanping Sun, William B. Isaacs, Jianjie Ma, Xuehong Xu

ABSTRACT

Background: Calcium signaling are conserved from invertebrates to vertebrates and plays critical roles in many molecular mechanisms of embryogenesis and postnatal development. As a critical component of the signaling pathway, the RyR medicated calcium-induced calcium release signaling system, has been well studied along with their regulator FK506-binding protein 12 (FKBP12/Calstabin). Lack of FKBP12 is known to result in lethal cardiac dysfunction in mouse. However, precisely how FKBP12 is regulated and effects calcium signaling in Drosophila melanogaster remains largely unknown. Results: In this study, we identified both temporal and localization changes in expression of DmFKBP12, a translational and transcriptional regulator of Drosophila RyR (DmRyR) and FKBP12, through embryonic development. DmFKBP12 is first expressed at the syncytial blastoderm stage and undergoes increased expression during the cellular blastoderm and early gastrulation stages. At late gastrulation, DmFKBP12 expression begins to decline until it reaches homeostasis, which it then maintains throughout the rest of development. Throughout these described changes in expression, DmFKBP12 mRNA remain stable, which indicates that protein dynamics are attributed to regulation at the mRNA to protein translation level. In addition to temporal changes in expression, dynamic expression profiles during Drosophila development also revealed DmFKBP12 localization. Although DmFKBP12 is distributed evenly between the anterior to posterior poles of the blastoderm egg, the protein is expressed more strongly in the cortex of the early Drosophila gastrula with the highest concentration found in the basement membrane of the cellular blastoderm. Fertilized egg, through the profile as under-membrane cortex distribution concentering onto basement at cellular blastoderm, to the profile as three-gem layer localization in primitive neuronal and digestion architecture of early Drosophila gastrula. By late gastrulation, DmFKBP12 is no longer identified in the yolk or lumen of duct structures and has relocated to the future brain (suboesophageal and supraesophageal ganglions), ventral nervous system, and muscular system. Throughout these changes in distribution, in situ DmFKBP12 mRNA monitoring detected equal distribution of DmFKBP12 mRNA, once again indicating that regulation of DmFKBP12 occurs at the translational level in Drosophila development. Conclusion: As a critical regulator of the DmRyR-FKBP complex, DmFKBP12 expression in Drosophila fluctuates temporally and geographically with the formation of organ systems. These finding indicate that DmFKBP12 and RyR associated calcium signaling plays an essential role in the successful development of Drosophila melanogaster. Further study on the differences between mammalian RyR-FKBP12 and Drosophila DmRyR-FKBP12 can be exploited to develop safe pesticides. More... »

PAGES

8

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13578-019-0270-6

DOI

http://dx.doi.org/10.1186/s13578-019-0270-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111259479

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30637096


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416 https://www.grid.ac/institutes/grid.261331.4 schema:alternateName The Ohio State University
417 schema:name National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 710062, Xi’an, China
418 Ohio State University School of Medicine, 43210, Columbus, OH, USA
419 rdf:type schema:Organization
420 https://www.grid.ac/institutes/grid.43169.39 schema:alternateName Xi'an Jiaotong University
421 schema:name College of Pharmacy, Xi’an Medical University, 710062, Xi’an, China
422 rdf:type schema:Organization
 




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