XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Zhihua Xiao, Yijun Wang, Hao Ding

ABSTRACT

Objective: The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. Methods: The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p. Results: In this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B. Conclusions: Our data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC. More... »

PAGES

6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13578-018-0269-4

DOI

http://dx.doi.org/10.1186/s13578-018-0269-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111159824

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30627419


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