Plant genome editing with TALEN and CRISPR View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Aimee Malzahn, Levi Lowder, Yiping Qi

ABSTRACT

Genome editing promises giant leaps forward in advancing biotechnology, agriculture, and basic research. The process relies on the use of sequence specific nucleases (SSNs) to make DNA double stranded breaks at user defined genomic loci, which are subsequently repaired by two main DNA repair pathways: non-homologous end joining (NHEJ) and homology directed repair (HDR). NHEJ can result in frameshift mutations that often create genetic knockouts. These knockout lines are useful for functional and reverse genetic studies but also have applications in agriculture. HDR has a variety of applications as it can be used for gene replacement, gene stacking, and for creating various fusion proteins. In recent years, transcription activator-like effector nucleases and clustered regularly interspaced palindromic repeats (CRISPR) and CRISPR associated protein 9 or CRISPR from Prevotella and Francisella 1 have emerged as the preferred SSNs for research purposes. Here, we review their applications in plant research, discuss current limitations, and predict future research directions in plant genome editing. More... »

PAGES

21

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13578-017-0148-4

    DOI

    http://dx.doi.org/10.1186/s13578-017-0148-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1084955116

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28451378


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